scholarly journals Haematological and blood chemistry changes in ewes and lambs following supplementation with vitamin E and selenium

1978 ◽  
Vol 40 (2) ◽  
pp. 193-203 ◽  
Author(s):  
G. M. J. Horton ◽  
W. L. Jenkins ◽  
Rosemarie Rettenmaier

1. Four methods of vitamin E and selenium supplementation were evaluated using thirty-nine pregnant ewe-lambs fed on a ration containing 0.043 mg Se/kg and 25 mg vitamin E/kg. Treatments were control, fortified mineral mix (ESe salt) (300 mg vitamin E, 3 mg Se), ruminal Se pellets (505 mg Se), drench (300 mg vitamin E, 3 mg Se) and intramuscular injection (600 mg vitamin E, 3 mg Se). Only ewes were supplemented, commencing approximately 50 d before parturition.2. Birth weights were similar for all treatments and live-weight gains of lambs to 56 d of age were improved in all supplemented groups (P < 0.05). There were no clinical cases of nutritional muscular dystrophy.3. Se concentrations in whole blood were more than doubled in both lambs and ewes drenched or injected; responses to ESe salt and pellets were much smaller.4. Plasma tocopherol levels were increased in injected dams and their lambs (P < 0.001).5. Haemoglobin concentration and erythrocyte counts were significantly higher (P < 0.01) in control ewes and lambs than in treated lambs.6. Lactate dehydrogenase (EC 1.1.1.27), creatine kinase (EC 2.7.3.2) and aspartate aminotransferase (EC 2.6.1.1) activities were increased in lambs from control, ESe salt and pellet groups (P < 0.001). Glutathione peroxidase (EC 1.1.1.1.9) activity responded to Se supplementation in both ewes and their lambs (P < 0.001) and the response was highest in the injected group, followed, in order, by the drench, pellet, ESe salt and control groups.7. These studies indicated that in terms of the haematological and blood chemistry changes investigated, the intramuscular injection was most effective, followed by the oral drench. Ruminal pellets and fortified salt were less satisfactory.

1959 ◽  
Vol 39 (2) ◽  
pp. 202-208 ◽  
Author(s):  
M. A. MacDonald ◽  
S. B. Slen

In this study effects of estradiol and testosterone injections and thyroidectomy on muscle fibre diameters of ewe and wether yearlings were determined. Forty-eight sheep, half of which were ewes and half wethers, were subdivided into estradiol, testosterone, and control groups containing equal numbers of each sex. Half of the animals within each sex group and treatment group were thyroidectomized. Biweekly rates of intramuscular injection of estradiol and testosterone were 5 mg. and 175 mg., respectively. All animals received the same amount of feed per day throughout a 195-day experimental period.Live weight gain, carcass weight, and percentage dry matter of m. longissimus dorsi tissue were not influenced significantly by hormone injection, thyroidectomy, or sex. Estradiol injections significantly increased (p <.01) muscle fibre diameters of thyroidectomized ewes and wethers but the animals with thyroids were unaffected. Testosterone injections failed to affect muscle fibre diameters. Differences in muscle fibre diameters could not be explained by differences in percentage moisture content of muscle tissue.


2020 ◽  
Vol 14 (4) ◽  
pp. 99-103
Author(s):  
V. I. Kolesnikov

The purpose of the research is studying the efficacy of Eprimek (Eprinomectin) against gastrointestinal nematodes in sheep.Materials and methods. A commercial experiment to study the antiparasitic efficacy of Eprimek was carried out in June 2020 on 300 lambs of the North Caucasian breed in a private flock of Filimonovskaya Village, Izobilnensky District, the Stavropol Territory, which were divided into two groups. The experimental group of lambs (290 animals) was injected Eprimek subcutaneously at the earset at a dose of 1 ml/50 kg of live weight (10 mg of Eprinomectin in 1 ml), and 10 lambs were not treated; they were used as control. We collected feces from the lambs of the experimental and control groups before administration of the drugs and after 15 and 30 days. Fecal samples were examined by the flotation technique with a saturated solution of ammonium nitrate with counting nematode eggs in 1 g of feces. The results were processed statistically.Results and discussion. Eprimek showed a decrease in the number of excreted helminth eggs from 225.1±28.2 to 4.1±2.3 in production environment at a dose of 1 ml/50 kg of live weight, according to coprological studies on the 15th day after treatment in the experimental group of lambs. The efficacy was 98.2%, and 70% of the animals were free from the infection. The intensity of infection of the control lambs by gastrointestinal nematodes was 131–151 eggs per 1 g of feces at 100% prevalence.


2020 ◽  
pp. 61-65
Author(s):  
И.Ю. Постраш ◽  
И.В. Сучкова ◽  
Е.Г. Скворцова ◽  
О.В. Филинская ◽  
А.В. Мостофина

Изучение влияния пробиотического препарата ЭМ-курунга на приросты, анализы крови и помёта птиц проведено на примере цыплят техасских белых перепелов. В качестве объекта исследований использовались цыплята перепелов, начиная с суточного возраста. Средняя масса цыплят на начало эксперимента составила 9,2±0,12 г. Цыплят забивали в возрасте 90 суток. Установлено, что введение в рацион цыплят пробиотического препарата ЭМ-курунга в количестве 0,2 мл на 1 кг живого веса увеличивает сохранность молодняка на 14,8% и приросты птенцов – на 10%. Сравнительный анализ биохимических данных опытной и контрольной групп птицы показал, что введение в рацион опытной группы пробиотика практически не повлияло на белковый обмен. Общий белок у опытной птицы был недостоверно меньше, по сравнению с контролем, на 1,25%, та же картина наблюдалась по уровню альбуминов – меньше на 1,9%, глобулинов – на 1%. Сравнительный анализ показателя кислотности кала птиц опытной и контрольной групп показал достоверное различие. Так, у контрольной группы рН кала имел среднее значение 6,5, у перепелов опытной группы – 7,5. Таким образом, у опытной птицы, в сравнении с контрольной, активная кислотность кишечного содержимого достоверно сдвинулась в слабощелочную сторону, что свидетельствует о благоприятном воздействии пробиотика на микрофлору желудочно-кишечного тракта перепелов, снижая уровень патогенной микрофлоры, активно развивающейся в кислой среде. The study of the effect of the probiotic preparation EM-Kurunga on gains, blood tests and poultry litter was carried out using the example of White Texas quail chickens. Quail chickens starting at daily age were used as the subject of research. The average weight of chickens at the beginning of the experiment was 9.2±0.12 g. Chickens were slaughtered at the age of 90 days. It was established that the introduction of the probiotic preparation EM-Kurunga into the diet of chickens in the amount of 0.2 ml per 1 kg of live weight increases the safety of young animals by 14.8% and the gains of poults – by 10%. A comparative analysis of the biochemical data of the experimental and control groups of poultry showed that the introduction of the probiotic into the diet of the experimental group had practically no effect on protein metabolism. The total protein in the experimental poultry was insignificantly less than in the control by 1.25%, the same pattern was observed in the level of albumin – less by 1.9%, globulins – by 1%. Comparative analysis of the acidity index of poultry feces from the experimental and control groups showed a significant difference. So, in the control group the pH of feces had an average value of 6.5, in the quails of the experimental group – 7.5. Thus, in the experimental poultry in comparison with the control one the active acidity of the intestinal contents significantly shifted to the slightly alkaline side, which indicates a favorable effect of the probiotic on the microbiota of the quail gastrointestinal tract, reducing the level of pathogenic microflora actively developing in an acidic condition.


Author(s):  
A. Korovushkin ◽  
S. Nefedova ◽  
Y. Yakunin ◽  
R. Baryshev

Статья посвящена исследованию использования в кормлении карпов (Cyprinus carрio Linnaeus) леонардита. У рыб его эффективность незаслуженно мало изучена. Комбикорм, в состав которого входит леонардит, является эффективным в получении крупного посадочного материала карпов, обеспечивает необходимый для товарного рыбоводства рост и развитие рыб, выращиваемых в установке замкнутого водообеспечения (УЗВ). Гуминовые кислоты из леонардита, проявляют свойства универсальной составляющей для трофики рыб, способной улучшить рыбопродуктивность, определяющую успешное ведение аквакультуры. Исследование рыбоводнобиологических показателей сеголетков, подращиваемых в УЗВ до возраста 90 суток, с целью получения крупного посадочного материала, при использовании в их рационе не модифицированных микропористых гуминовых кислот, составляющих основу леонардита, показало, что эти органические вещества эффективны, они стимулируют ростовые показатели молоди карпов. Так, при сравнении опытной и контрольной групп по основным показателям, все они оказались выше у тех сеголетков, кто в рационе получал модифицированный комбикорм с леонардитом. За период выращивания сеголетков до возраста 90 суток при плотности посадки в одном бассейне основного УЗВ в среднем 500 штук, отличие между рыбами из опытной и контрольной групп в пользу первой составило: по выживаемости 3, живой массе к концу эксперимента 13,9, абсолютному приросту 13,9, среднесуточному приросту 13,5, относительному приросту 9 562,0. Исходя из результатов эксперимента в УЗВ, обогащение продуктивных качеств комбикорма производства Лимкорм , используемого в кормлении посадочного материала карпов, гуминовыми кислотами из леонардита, наблюдается повышение рыбоводнобиологических показателей в среднем на 13,4 , что экономически выгодно хозяйствам, занимающимся прудовой аквакультурой.The article is devoted to use in feeding of carp (Cyprinus carрio Linnaeus) of leonardite. Fish its effectiveness undeservedly little studied. Compound feed, which includes leonardite, is effective in obtaining a large planting material of carp, provides the necessary for commercial fish farming growth and development of fish grown in a closed water supply unit (WSU). Humic acids from leonardite exhibit the properties of a universal component for fish trophies, which can improve fish productivity, which determines the successful management of aquaculture. A study of the fishbiological parameters of fingerlings, were grown produce low WSU until the age of 90 days, with the aim of obtaining large planting material, when used in their diet is not modified microporous humic acids that form the basis of leonardite, showed that these organic substances effectively, they stimulate the growth indices of juvenile carp. Thus, when comparing the experimental and control groups on the main indicators, all of them were higher in those juveniles who received modified feed with leonardite in the diet. During the period of growing juveniles up to the age of 90 days at the density of planting in one basin of the main WSU on average 500 pieces, the difference between the fish from the experimental and control groups in favor of the first was: survival rate of 3, live weight by the end of the experiment 13.9, absolute growth of 13.9, average daily growth of 13.5, relative growth of 9 562.0. Based on the results of the experiment in WSU, enrichment of productive qualities of feed production Limcom used in the feeding of planting material carp, humic acids extracted from lignite, an increase of the fishbiological indicators in average by 13.4 , which is economically beneficial to farmers engaged in pond aquaculture.


2012 ◽  
Vol 48 (No. 1 - 2) ◽  
pp. 9-17
Author(s):  
I. Langrová ◽  
I. Jankovská ◽  
M. Borovský

Moxidectin administered in January or February at a single dose was tested for efficacy in horses on two farms for 12 and 11 months, respectively. Horses were infected with cyathostomes naturally in the previous grazing period. Forty horses of farm 1 and 20 horses of farm 2 were used in controlled tests to evaluate the efficacy of moxidectin 2% gel formulation at the dosage 0.4 mg moxidectin per kg of live weight, ivermectin commercial paste formulation at the dosage 0.2 mg ivermectin per kg of live weight, mebendazole and fenbendazole commercial paste formulation at the dosage both 7.5 mg mebendazole and fenbendazole per kg of live weight, all applied orally. Three control groups of 10 horses each (farm 1) were treated twice a year with ivermectin and benzimidazoles, respectively. Individual faecal egg counts, faecal cultures and larval differentiation were performed. Moxidectin had more prolonged and greater suppressive effects on the post-treatment reappearance and magnitude of strongyle egg counts than did ivermectin or benzimidazoles. In the moxidectin treated group (M1) strongyle eggs were seen for the first time in April and a slight increase in the mean count of eggs per gram of faeces (EPG) was observed during the rest of the season. Litter larval counts significantly reflected levels of exposure during the tested season. Twenty animals of farm 2 were allocated into two groups of ten horses each based on pre-treatment eggs per gram (EPG) counts (moxidectin treated group and control group). In the moxidectin treated group mean egg counts remained very low throughout the study. A plateau was reached by autumn, with egg counts ranging from 74 to 145 EPG. The faecal egg counts of moxidectin treated group (M2) were significantly higher in March, April, May and June.


Author(s):  
A. Magaya ◽  
S. Mukaratirwa ◽  
A.L. Willingham ◽  
N. Kyvsgaard ◽  
S. Thamsborg

A study was carried out to determine the epidemiology of gastrointestinal nematodes in indigenous Tuli cattle and the effect of dietary protein supplementation and anthelmintic treatment on productivity in young growing cattle. Forty steers with an average age of 18 months were divided into 4 groups; 1) fenbendazole (slow release bolus) and cottonseed meal (FCSM group), 2) fenbendazole (FBZ group), 3) cottonseed meal (CSM group) and 4) control (no cottonseed meal and no fenbendazole) (control group). Performance parameters measured included wormeggs per gram of faeces (EPG), packed cell volume (PCV), albumin and live-weight gain. Results showed that faecal worm egg counts were lower and PCV was higher in the FCSM and FBZ groups than in the CSM and control groups (P < 0.01). Weight gains were higher in the CSMand FCSM groups than in the FBZ and control groups (P < 0.05). The cost benefits of anthelmintic treatment and dietary supplementation were apparent in this study. The improved growth performance of the FCSM, FBZ and CSM groups reflected a financial gain over the controls on termination of the study. The dominant genera of gastrointestinal nematodes on faecal culture, pasture larval counts and necropsy were Cooperia and Haemonchus. The incidences of Trichostrongylus, Oesophagostomum and Bunostomum were low.


2019 ◽  
Vol 70 (1) ◽  
pp. 1347
Author(s):  
A. KHODADADI ◽  
A. HAGHIGHI ◽  
H. MALEKINEJADH ◽  
A. TUKMECHI ◽  
M. AFSHARNASAB

The aim of this study was to evaluate the effect of a prebiotic (Celmanax®) containing yeast cell wall with mannan oligosaccharides on the haematological and serum biochemical parameters in rainbow trout. Three levels of prebiotic (0, 0.1, 0.5 and 1 %) were mixed into pellets. Fish (19.08±1.45 g) were fed a supplemented commercial diet for 60 days. Blood samples were colected from the onset and on days 30 and 60 of the trial to measure the haematological and serum biochemical parameters in rainbow trout. The results showed significant differences in haemoglobin, mean corpuscular volume,mean corpuscular haemoglobin concentration, white blood cells and neutrophil count between control and all test groups (p<0.05). The highest and the lowest white blood cells and neutrophil count (on day 60) were observed in the 0.1 %, prebiotic-received and control groups, respectively. Also, the result showed significant differences in Alkaline phosphatase enzymes, serum glutamic oxaloacetic transaminase, Serum glutamic-pyruvic transaminase, between the test and control groups(p<0.05) while non-significant elevation of blood urea nitrogen, creatinine and total protein levels was found in the Celmanax®-received groups (p>0.05). These results suggest that the Celmanax® supplementation enhances white blood cells and neutrophil count, and changes some biochemical parameters in rainbow trout.


1959 ◽  
Vol 31 (1) ◽  
pp. 141-145
Author(s):  
S. Haaranen

6 heifers of 4—9 months suffering from indigestion were given oat meal containing 80 mg of Aureomycin for 43 days. 6 similar heifers, serving as controls, were kept on the same diet and in the same shed. The average daily additional growth of the group receiving aureomycin was 285 g; the corresponding additional growth of the control groups was 108 g. The average increases for the test and control group, respectively, were: live weight 14.45 kg/6.48 kg, length 9.33 cm/3.33 cm, chest circumference 6.50 cm/4.33 cm, height of withers 1.8 cm/1.2 cm, pelvic breadth at the tubera coxae 1.7 cm/0.7 cm. Serum calcium and copper fell and potassium rose in the material as a whole. There were no statistically significant differences between the test and control groups except in Na, which rose in the test and fell the control groups. In view of the small size of each group and the nevertheless nearly significant difference in average weights (0.05 < P < 0.1), it would seem that aureomycin additive in the feed was beneficial for the heifers in these conditions. Economic consiferations will decide whether it is generally advisable to resort to aureomycin additive. The blood changes were regarded as being due to the transfer of the animals from the cold winter air indoors to a warm shed.


2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Iraj Shahramian ◽  
Kaveh Tabrizian ◽  
Mahdi Afshari ◽  
Ali Bazi ◽  
Elaheh Bahmadisani

Background: Hepatitis A virus (HAV) infection is a widespread disease with no specific treatment. Objectives: In the present study, we investigated the effects of vitamin E in the treatment of acute HAV infection in children. Methods: This clinical trial study was conducted on 142 patients with acute HAV infection referred to Amir-Al-Momenin Hospital of Zabol during February 2016-August 2017. The patients were randomly divided into two groups of intervention (Vit E, n = 71) and control (no medication, n = 71). Liver enzymes were monitored during a six-month period. Results: The mean ages of the patients were 8.4 ± 2.5 and 9 ± 4.3 years in the control and intervention groups, respectively. Male participants constituted 36 (50.7%) and 35 (49.3%) in the control and intervention groups, respectively. In both the treatment and control groups, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) significantly decreased over six months during the study (P < 0.001). AST level (IU/L) was significantly lower in the children who received vitamin E than controls at one-month (P = 0.01), two-month (P = 0.002), three-month (P = 0.005), four-month (P < 0.001), and six-month (P = 0.002) post-treatment periods. There was no significant difference comparing ALT between the intervention and control groups except for two months post-treatment (P = 0.01). Conclusions: Our study showed that the administration of vitamin E in children with acute hepatitis A can accelerate liver enzyme normalization.


2009 ◽  
Vol 52 (3) ◽  
pp. 309-320
Author(s):  
M. Gabryszuk ◽  
K. Horbańczuk ◽  
J. Klewiec

Abstract. The experiment was conducted on 80 Polish Merino ewes, their 82 lambs and 40 fattening rams. Merino ewes were divided into two groups: experimental (E) and control (C), each composed of 40 animals. Four weeks before lambing, 1 day, 3 weeks and 6 weeks after lambing 40 ewes of the experimental (E) group received intramuscular injection of 5 ml 0.1 % Na2SeO4 (selenium [Se]: 2.09 mg), 10 ml 10 % ZnSO4 (Zinc [Zn]: 227 mg) and 250 mg vitamin E (α-tocopherol), and control group (C) – no received. From birth all the lambs were maintained with their dams and then weaned at the age of 8 weeks, placed in individual straw-bedded pens and fattened individually with a granulated concentrate mixture until reaching a body weight about 32 kg. During fattening 20 lambs from experimental group (E) was given per os 1 ml 0.1 % Na2SeO4, 3 ml 10 % ZnSO4 and 60 mg vitamin E (α-tocopherol) daily, and control group (C) – no administered. The levels of Se, Zn and vitamin E in the blood plasma of ewes and ram- lambs were within the reference values. The lambs born by the treated ewes (E) experienced much lower body live weight at birth (P≤0.05), body weight on day 28 and 56 (P≤0.01), compared to lambs born from group (C). Also the daily live weight gain from birth to 4 week and from birth to 8 week were significantly lower (P≤0.05) in lambs born by the treated ewes. Se, Zn and vitamin E treated rams contained less fat in leg (P≤0.05), less fat thickness over ribs (P≤0.01) and less fat thickness over loin-eye than non treated rams.


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