Effect of forage type and proportion of concentrate in the diet on milk fatty acid composition in cows given sunflower oil and fish oil

2005 ◽  
Vol 80 (2) ◽  
pp. 225-238 ◽  
Author(s):  
K. J. Shingfield ◽  
C. K. Reynolds ◽  
B. Lupoli ◽  
V. Toivonen ◽  
M. P. Yurawecz ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Fish Oil ◽  
Acid Composition ◽  
Sunflower Oil ◽  
Milk Fat ◽  
Milk Fatty Acid ◽  
Grass Silage ◽  
Forage Type

AbstractBased on the potential benefits ofcis-9,trans-11 conjugated linoleic acid (CLA) for human health there is a need to develop effective strategies for enhancing milk fat CLA concentrations. In this experiment, the effect of forage type and level of concentrate in the diet on milk fatty acid composition was examined in cows given a mixture of fish oil and sunflower oil. Four late lactation Holstein-British Friesian cows were used in a 4 × 4 Latin-square experiment with a 2 × 2 factorial arrangement of treatments and 21-day experimental periods. Treatments consisted of grass (G) or maize (M) silage supplemented with low (L) or high (H) levels of concentrates (65 : 35 and 35 : 65; forage : concentrate ratio, on a dry matter (DM) basis, respectively) offered as a total mixed ration at a restricted level of intake (20 kg DM per day). Lipid supplements (30 g/kg DM) containing fish oil and sunflower oil (2 : 3 w/w) were offered during the last 14 days of each experimental period. Treatments had no effect on total DM intake, milk yield, milk constituent output or milk fat content, but milk protein concentrations were lower (P< 0.05) for G than M diets (mean 43.0 and 47.3 g/kg, respectively). Compared with grass silage, milk fat contained higher (P< 0.05) amounts of C12:0, C14:0, trans C18:1and long chain ≥ C20 (n-3) polyunsaturated fatty acids (PUFA) and lower (P< 0.05) levels of C18:0and trans C18:2when maize silage was offered. Increases in the proportion of concentrate in the diet elevated (P< 0.05) C18:2(n-6) and long chain ≥ C20 (n-3) PUFA content, but reduced (P< 0.05) the amount of C18:3(n-3). Concentrations oftrans-11 C18:1in milk were independent of forage type, but tended (P< 0.10) to be lower for high concentrate diets (mean 7.2 and 4.0 g/100 g fatty acids, for L and H respectively). Concentrations oftrans-10 C18:1were higher (P< 0.05) in milk from maize compared with grass silage (mean 10.3 and 4.1 g/100 g fatty acids, respectively) and increased in response to high levels of concentrates in the diet (mean 4.1 and 10.3 g/100 g fatty acids, for L and H, respectively). Forage type had no effect (P> 0.05) on total milk conjugated linoleic acid (CLA) (2.7 and 2.8 g/100 g fatty acids, for M and G, respectively) orcis-9,trans-11 CLA content (2.2 and 2.4 g/100 g fatty acids). Feeding high concentrate diets tended (P< 0.10) to decrease total CLA (3.3 and 2.2 g/100 g fatty acids, for L and H, respectively) andcis-9,trans-11 CLA (2.9 and 1.7 g/100 g fatty acids) concentrations and increase milktrans-9,cis-11 CLA andtrans-10,cis-12 CLA content. In conclusion, the basal diet is an important determinant of milk fatty acid composition when a supplement of fish oil and sunflower oil is given.

scholarly journals Influence of fish oil, palm oil and glycerol on milk fatty acid composition and metabolism in cows during early lactation

2012 ◽  
Vol 55 (6) ◽  
pp. 540-551 ◽  
Author(s):  
R. Kupczyński ◽  
M. Kuczaj ◽  
M. Szołtysik ◽  
T. Stefaniak
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Fish Oil ◽  
Acid Composition ◽  
Palm Oil ◽  
Milk Fat ◽  
Vaccenic Acid ◽  
Milk Fatty Acid ◽  
Glycerol Concentration

Abstract. The aim of the study was to determine an influence of diet additives in a form of protected palm oil, protected fish oil or unprotected fish oil with glycerol in Polish Holstein-Friesian cows on milk fatty acid composition, metabolism, milk yield and milk composition. Milk production, milk fat, and milk protein did not differ statistically between the groups. A significant increase (P<0.01) in glucose level in blood was noted after application of unprotected fish oil with glycerol. The lowest concentration of β-hydroxybutyrate and non-esterified fatty acids, with the highest cholesterol and triacylglycerol concentration was observed in protected fish oil. An increase in the content of long-chain acids was observed in milk fat of cows receiving protected fish oil when compared to the group receiving palm oil and unprotected fish oil with glycerol. Concentration of trans-vaccenic acid (TVA) was higher (P<0.01) in protected fish oil and unprotected fish oil with glycerol when compared to palm oil group. These changes were corresponded by concentration of cis-9, trans-11 conjugated linoleic acid (CLA) (1.71, 1.68 and 0.61 g/100 g of total fatty acids, respectively). Irrespectively of the form of fish oil administration, an increase in milk eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) content was noted. The present experiment provides evidence that milk fatty acids can be manipulated via dietary fish oil or unprotected fish oil and glycerol application.

scholarly journals Dietary fish oil supplements depress milk fat yield and alter milk fatty acid composition in lactating cows fed grass silage-based diets

2015 ◽  
Vol 98 (8) ◽  
pp. 5653-5671 ◽  
Author(s):  
P. Kairenius ◽  
A. Ärölä ◽  
H. Leskinen ◽  
V. Toivonen ◽  
S. Ahvenjärvi ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Fish Oil ◽  
Acid Composition ◽  
Milk Fat ◽  
Milk Fatty Acid ◽  
Grass Silage ◽  
Lactating Cows ◽  
Dietary Fish ◽  
Fat Yield

Effect of forage conservation method, concentrate level and propylene glycol on the fatty acid composition and vitamin content of cows' milk

2005 ◽  
Vol 72 (3) ◽  
pp. 349-361 ◽  
Author(s):  
Kevin J Shingfield ◽  
Pirjo Salo-Väänänen ◽  
Eero Pahkala ◽  
Vesa Toivonen ◽  
Seija Jaakkola ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Ascorbic Acid ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Milk Fat ◽  
Milk Fatty Acid ◽  
Vitamin Content ◽  
Concentrate Level ◽  
Conservation Method

Based on potential health benefits, there is a need to develop effective strategies for enhancing milk fat concentrations of cis-9 18[ratio ]1, 18[ratio ]3 n-3 and conjugated linoleic (CLA) content in milk without compromising the sensory or storage characteristics of processed milk or dairy products. Sixteen Finnish Ayrshire dairy cows were used in a cyclic change-over experiment with four 21-d experimental periods and a 4×2×2 factorial arrangement of treatments to evaluate the effects of forage conservation method, concentrate level and supplements of propylene glycol (PG), and their interactions on milk fatty acid composition and vitamin content. Experimental treatments consisted of four conserved forages offered ad libitum, supplemented with two levels of a standard concentrate (7 or 10 kg/d) and PG (0 and 210 g/d) fed as three equal meals. Primary growths of timothy and meadow fescue sward were conserved by ensiling with none (NA), an inoculant enzyme preparation (IE) or a formic acid based (FORM) additive or as hay 1 week later. Conservation of grass by drying rather than ensiling resulted in lower forage 18[ratio ]2n-6, 18[ratio ]3n-3, total fatty acid and fat-soluble vitamin concentrations. In spite of lower intakes, milk fat 18[ratio ]2n-6 and 18[ratio ]3n-3 content was higher (P<0·05) for hay than for silage diets (12·1, 9·6, 9·6 and 9·3 and 5·00, 3·51, 4·27 and 2·93 g/kg total fatty acids, for hay, NA, IE and FORM silages, respectively). Forage conservation method had no clear effects on milk trans 18[ratio ]1 or CLA content. Compared with silage, hay diets resulted in milk containing lower (P<0·001) riboflavin, α-tocopherol and β-carotene concentrations, but had no effect on ascorbic acid, thiamine, pyridoxine or retinol content. Feeding more concentrates had no effect on milk fatty acid composition or milk vitamin content, other than lowering (P<0·001) 16[ratio ]0 concentrations from 348 to 338 g/kg fatty acids. Supplements of PG led to small (P<0·05) increases in milk 13[ratio ]0 anteiso and 15[ratio ]0 content from 1·06 and 11·3 to 1·22 and 12·6 g/kg fatty acids and reduced (P<0·05) the concentrations of ascorbic acid (16·1 v. 15·1 g/kg milk).

scholarly journals Ruminal parameters and fatty acid composition of omasal digesta and milk in cows fed sugarcane-based diets supplemented with sunflower oil

2020 ◽  
pp. 2317-2334
Author(s):  
Fernando César Ferraz Lopes ◽  
Shirley Motta de Souza ◽  
Sebastião de Campos Valadares Filho ◽  
Marco Antônio Sundfeld da Gama ◽  
Luciana Navajas Rennó
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Sunflower Oil ◽  
Milk Fat ◽  
Volatile Fatty Acids ◽  
Linear Increase ◽  
Neutral Detergent Fiber ◽  
Apparent Digestibility

This study evaluates the intake and digestion of nutrients, parameters of rumen fermentation and degradation, omasal digesta and milk fatty acid composition, productive performance, and the concentration of serum metabolites in cows fed 600 g kg-1 sugarcane-based diets containing 0 (control), 15, 30, and 45 g kg-1 sunflower oil (SO) on a dry matter (DM) basis. Four rumen-cannulated Holstein x Gyr cows yielding 15±5 kg day-1 with 110±10 days in milk were allocated in a 4 x 4 Latin square design. Data were analyzed using mixed models, and significant differences were declared at P < 0.05. There was no effect of SO on the intake and apparent digestibility of DM, crude protein, neutral detergent fiber (NDF) and nonfibrous carbohydrates, but there was a linear increase in the intake and digestibility of ether extract. Dietary SO levels did not alter the ruminal degradability parameters for DM and NDF, rumen pH and contents of ammonia N, acetate, propionate and volatile fatty acids. Milk fat content and yield were linearly decreased, whereas a linear increase in milk protein content was observed in response to increasing levels of SO, but with no effect on milk yield. Linear reductions in palmitic and ?-linolenic acid contents, a linear increase in trans-10 C18:1 and elaidic acids, and a quadratic effect on vaccenic and rumenic acids were observed in omasal digesta of cows fed increasing levels of SO. Overall, up to 45 g kg-1 SO can be included on DM of chopped sugarcane-based diets without reducing consumption, apparent digestibility and rumen degradability of DM and fiber. Supplementing chopped sugarcane-based diets with 30 to 45 g kg-1 SO (DM basis) promotes milk fat depression due to the inhibition of mammary lipogenesis by specific rumen-derived fatty acid intermediates of the biohydrogenation of unsaturated C18 fatty acids. The inclusion of 15 to 45 g kg-1 SO in chopped sugarcane-based diets improves the nutritional quality of milk fat, with increases in the levels of oleic, vaccenic and rumenic acids, beneficial to human health, and a reduction in the levels of the hypercholesterolemic lauric, myristic and palmitic acids.

The effect of duration of feeding oilseeds to dairy cows on the persistency of response in milk fatty acid composition

2003 ◽  
Vol 2003 ◽  
pp. 22-22
Author(s):  
D. I. Givens ◽  
C. Rymer ◽  
A.R. Moss ◽  
R. Allison
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Dairy Cows ◽  
Acid Composition ◽  
Milk Fat ◽  
Extended Period ◽  
Monounsaturated Fatty Acids ◽  
Milk Fatty Acid ◽  
Human Diet

There is much interest in the manipulation of the fatty acid composition of milk fat so as to improve its health characteristics in the human diet. In an earlier study there was an indication that the concentration of fatty acids in milk may change with time when feeding whole oilseeds rich in polyunsaturated fatty acids (PUFA). In particular a tendency for an increase in CLA and C18:1 and a reduction in C18:2 and C18:3 concentrations with time were seen. The present study was therefore undertaken to examine the changes in the fatty acid composition of milk from high yielding dairy cows fed diets rich in monounsaturated fatty acids (MUFA) or PUFA over an extended period.

scholarly journals Associations of the SCD1 gene SNP with fatty acids composition of Holstein cows

2020 ◽  
Vol 27 ◽  
pp. 00060
Author(s):  
Natalia Yu. Safina ◽  
Shamil K. Shakirov ◽  
Rustam Kh. Ravilov ◽  
Gazimzyan S. Sharafutdinov
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Milk Fat ◽  
Mass Fraction ◽  
Holstein Cows ◽  
Allelic Polymorphism ◽  
Milk Fatty Acid ◽  
Significant Difference

The research presents the findings of DNA testing of allelic polymorphism by the PCR-RFLP method of the SCD1 gene and the relationship of genotypes with the milk fatty acid composition of Holstein cows of Tatarstan population. The research was conducted among 172 Holstein cows at the Integrated Agricultural Production Centre “Stud farm named after Lenin” in Atninsky district of the Republic of Tatarstan in 2017–2018. All genotypes of the SCD1 gene were identified through the research. The milk yield test showed that the maximum content of the mass fraction of fat was in animals with the TT genotype. The significant difference between the content of the mass fraction of fat in milk in individuals by the locus of the SCD1-Fsp4H I gene was: TT to CC – 0.32 % (p < 0.05) and TT to TC – 0.40 % (p < 0.01). The results of chromatography indicate the balance of the milk fatty acid composition of cows with the TT genotype and, as a consequence, its positive effect on the human body. Cows with a homozygous TT genotype are characterized by the optimum ratio of fatty acids in milk fat. The maximum value of the oxygen desaturation index is also observed in animals with the TT genotype by the SCD1 gene.

Effect of increased milking frequency and residual milk removal on milk production and milk fatty acid composition in lactating cows

2017 ◽  
Vol 84 (4) ◽  
pp. 453-463 ◽  
Author(s):  
Sabine Ferneborg ◽  
Lucia Kovac ◽  
Kevin J Shingfield ◽  
Sigrid Agenäs
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Milk Yield ◽  
Milk Fat ◽  
Major Influence ◽  
Composition Analysis ◽  
Milk Fatty Acid ◽  
Milking Frequency

It has been well established that milk yield is affected both by milking frequency and due to the removal of residual milk, but the influence of a combination of these factors is unclear. In this study, four mid-lactation cows were used in a 4 × 4 Latin square design to test the hypothesis that the effects of more frequent milking and residual milk removal on milk yield and composition are additive and alter milk fatty acid composition. Treatments comprised two or four times daily milking in combination with (or without) residual milk removal over a 96 h interval preceded by a 2 d pretreatment period and followed by a 8 d washout in each 14 d experimental period. Milk was sampled at each milking for the analysis of gross composition and SCC. Samples of available and residual milk collected on the last milking during each treatment period were collected and submitted for fatty acid composition analysis. Increases in milking frequency and residual milk removal alone or in combination had no effect on milk yield or on the secretion of lactose and protein in milk. However, residual milk removal during more frequent milking increased milk fat yield. Milking treatments had no major influence on the fatty acid composition of available milk, but resulted in rather small changes in the relative abundance of specific fatty acids, with no evidence that the additive effects of treatments were due to higher utilisation of preformed fatty acids relative to fatty acid synthesis de novo. For all treatments, fat composition of available and residual milk was rather similar indicating a highly uniform fatty acid composition of milk fat within the mammary gland.

scholarly journals Examination of the Persistency of Milk Fatty Acid Composition Responses to Fish Oil and Sunflower Oil in the Diet of Dairy Cows

2006 ◽  
Vol 89 (2) ◽  
pp. 714-732 ◽  
Author(s):  
K.J. Shingfield ◽  
C.K. Reynolds ◽  
G. Hervás ◽  
J.M. Griinari ◽  
A.S. Grandison ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Fish Oil ◽  
Dairy Cows ◽  
Acid Composition ◽  
Sunflower Oil ◽  
Milk Fatty Acid

scholarly journals The effect of dietary forage to concentrate ratio and forage type on milk fatty acid composition and milk fat globule size of lactating cows

2019 ◽  
Vol 102 (10) ◽  
pp. 8825-8838 ◽  
Author(s):  
Mari J. Jaakamo ◽  
Tytti J. Luukkonen ◽  
Piia K. Kairenius ◽  
Ali R. Bayat ◽  
Seppo A. Ahvenjärvi ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Milk Fat ◽  
Milk Fatty Acid ◽  
Lactating Cows ◽  
Globule Size ◽  
Milk Fat Globule ◽  
Fat Globule ◽  
Forage Type

scholarly journals Milk fatty acid composition of Holstein x Gyr dairy cows fed sugarcane-based diets containing citrus pulp supplemented with sunflower oil

2019 ◽  
Vol 40 (4) ◽  
pp. 1663
Author(s):  
Shirley Motta de Souza ◽  
Fernando César Ferraz Lopes ◽  
Sebastião de Campos Valadares Filho ◽  
Marco Antônio Sundfeld da Gama ◽  
Luciana Navajas Rennó ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Dairy Cows ◽  
Milk Production ◽  
Elaidic Acid ◽  
Sunflower Oil ◽  
Milk Fat ◽  
Milk Fatty Acid ◽  
Citrus Pulp

Sugarcane is a strategic roughage source for milk production in the tropics, and its supplementation with plant oils offers great potential for obtaining milk enriched with beneficial fatty acids, such as oleic (cis-9 C18:1), vaccenic (trans-11 C18:1), and rumenic (cis-9, trans-11 CLA) acids. The aim of this study was to evaluate the milk fatty acid composition of cows fed 60% chopped sugarcane-based diets containing citrus pulp and 0.0% (control), 1.5%, 3.0% and 4.5% sunflower oil on a dry matter basis. Twelve multiparous Holstein x Gyr dairy cows with an average milk production of 17±5 kg day-1 and 96±25 days in milk were allocated in a triplicate 4 x 4 Latin square design. There was no effect of dietary treatment on milk production, but sunflower oil supplementation linearly reduced the milk fat content and yield. The milk fat contents of lauric (C12:0), myristic (C14:0), and palmitic (C16:0) fatty acids were linearly reduced with increasing sunflower oil levels. There was a quadratic effect on the milk fat oleic, vaccenic, and rumenic acid contents, while the concentrations of elaidic acid (trans-9 C18:1), trans-10 C18:1 and trans-10, cis-12 CLA linearly increased in response to sunflower oil inclusion. Diet supplementation with sunflower oil linearly reduced both the atherogenicity and thrombogenicity indices and linearly increased the ratio between hypo- and hypercholesterolemic fatty acids in milk fat. The inclusion of up to 4.5% sunflower oil in 60% chopped sugarcane-based diets improved the nutritional quality of milk fat from Holstein x Gyr dairy cows as a result of the increased content of oleic, rumenic and vaccenic acids, which are beneficial to human health, and the concomitant reduction of hypercholesterolemic lauric, myristic and palmitic acids. However, there was an increase in the milk content of trans-10 C18:1 and elaidic acid, which are associated with deleterious effects on cardiovascular health.

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