Environmental DNA biomonitoring revealed species diversity of Cnidarian and Poriferan across Jakarta Bay and Seribu Islands National Park

Indonesia, as a country having a unique sea lane known as the Indonesian Archipelagic Sea Lanes (ASLs), has become one of the busiest countries in the world with varied shipping activities. These actions have the ability to facilitate the transmission of species (bio-invasion). Until recently, the number of global introduced species has increased, with negative consequences for environment and the economy. Environmental DNA (eDNA) approaches for detecting the presence of invasive species are currently receiving a lot of interest as a broad approach method in ecological research. As a result, the study used the eDNA technique to compare the quantity and variety of introduced species from the Cnidaria and Porifera Phyla, as well as to characterize their invasiveness status and possible presence in the waters of Jakarta Bay. Based on data from the inside Zone of National Park (ZI) and Outside Zone of National Park (ZO), the biological community composition, richness, and diversity were assessed (ZO). The mBrave workflow generated a total of 14,275 reads from high-throughput sequencing of amplicons from two zones, with 8,917 reads in ZI and 5,358 reads in ZO. Blackfordia virginica , Cordylophora caspia , and Ectopleura crocea were among the imported species included in the invasive category, with E. crocea having the highest abundance and being detected in both zones, with a total of 1300 reads, consisting of 1253 reads in ZI and 47 reads in ZO. Based on the findings, the eDNA methodology can be used as a biomonitoring and conservation method for invasive species.

Multivariate modelling of milk fatty acid profile to discriminate the forages in dairy cows’ ration

Although there are many studies on the importance of fatty acids (FA) in our diet and on the influence of dairy diets on FA metabolism, only a few investigate their predictive capacity to discriminate the type, amount and conservation method of farm forages. This research quantifies differences in milk FA concentrations and, using a supervised factorial discriminant analysis, assesses potential biomarkers when replacing maize with other silages, grass/lucerne hays or fresh grass. The statistical modelling identified three main clusters of milk FA profiles associated with silages, hays and fresh grass as dominant roughages. The main implication of a dairy cow feeding system based on poliphytic forages from permanent meadows is enhancing milk’s nutritional quality due to an increase in beneficial omega-3 polyunsaturated FA, conjugated linoleic acids and odd chain FA, compared to feeding maize silage. The study also identified a small but powerful and reliable pool of milk FA that can act as biomarkers to authenticate feeding systems: C16:1 c-9, C17:0, C18:0, C18:3 c-9, c-12, c-15, C18:1 c-9, C18:1 t-11 and C20:0.

A CASTLE IN A STATE OF LONG-TERM RUIN AND MEASURES AIMED AT ITS PRESERVATION

In the article, the definitions “castle in a state of long-term ruin” and “architectural revitalization” are given. A list of measures aimed at preserving architectural monuments from the standpoint of their application on castles in a state of long-term ruin is considered. The definitions characterizing permissible activities on architectural monuments specified in the Law of Ukraine “On the Protection of Cultural Heritage” and restoration State Building Standards, however, in theory and practice, much more concepts are used, since the regulated terms do not cover the entire range of possibilities for the preservation and development of architectural monuments. In their works, M. Bevz, A. Rybchynskyi, O. Plamenytska, P. Molski highlight measures for the preservation of architectural monuments. Analyzing the history of the founding of defensive castles, their further restructuring and decline, we form a definition: a castle in a state of long-term ruin is a castle, as a result of destruction during a siege or loss of its defensive functions in the 16th-17th centuries was abandoned without acquiring new owners fell and came to the state of ruin that we can observe today. Looking at conservation measures using the example of castles in a state of long-term ruin, we state the following positions: The conservation method is the main point for keeping castles in a state of long-term ruin. The restoration can only be applied fragmentarily, since a holistic restoration is akin to restoration, which is an unacceptable step, given the lack of sufficient information about the original appearance of the structure. Museumification is excellent, as a method of bringing a castle in ruins to a state suitable for an excursion visit, as well as designation, as a subspecies of museumification, accompanied by both information stands and tracing of foundations reveals the planning structure of the monument. Revitalization measures aimed at revitalizing the degraded space, and renovations, which give an authentic building a touch of novelty and emphasize the period of intervention in the monument are also important. In general, the idea of preserving the castle in a state of long-term ruin should be based on three main positions such as conservation, fragmentary restoration and revitalization, that is, preserving the authentic component and returning life to the object.

ILLUSORY RESTITUTION OF NOT PRESERVED ARCHITECTURAL OBJECTS – AN ANALYSIS OF METHODS AND A CASE STUDY

The article concerns the illusory presentation of non-existent architectural objects directly in the place of their original location. This specific conservation method is considered as a tool for disseminating knowledge about the original architectural landscape of places where it has undergone transformations. The authors believe that its implementation may contribute to the growth of the identity of cities and their inhabitants. The concept refers to augmented reality, but the authors use only optical means to create a virtual component, The article presents the results of theoretical analysis and practical experiments, pointing to the advantages and disadvantages of the method.

A unique archetype of conservation in Himachal Pradesh, western Himalaya, India

Owing to numerous emerging threats to biodiversity, its conservation has been of paramount importance in today’s world. Interestingly, many modern practices have been followed globally for the conservation of natural resources, yet traditional conservation practices that could set an excellent example need to be explored worldwide. Keeping this in view, the current communication aims to highlight a unique conservation method that has been practiced in the remote and cold-arid region of the state of Himachal Pradesh in the western Himalaya. Locally known as ‘Praja Mandal’, this indigenous system of conservation needs to be addressed and adopted nationwide with an eye towards a sustainable ecosystem.

EFFECT OF IRRADIATION IN HONEY INOCULATED WITH Bacillus sporothermodurans

Honey is a natural viscous product widely used for nutritional and medicinal purposes. Although it’s a food that presents a high degree of resistance against the growth of microorganisms is not a sterile medium. Spore-forming bacteria, such as Clostridium botulinum, are present in honey and it’s the only recorded source of food carrier of the agent that causes childhood botulism. The steps of honey processing do not include the heat treatment for its commercial sterility, due to the application of heat increase the presence of hydroxymethylfurfural. Food irradiation is a method of preservation that can be applied in the processing of honey by not physically altering the appearance, shape or temperature of the product. The objective of this work was to evaluate the effect of gamma irradiation on honey inoculated with Bacillus sporothermodurans spores. The inoculation of 106 CFU.mL-1 of lyophilized culture of this bacterium in the honey samples was carried out and the irradiation of the samples at 5, 10 and 15 kGy doses was carried out. After irradiation, the samples were incubated at 30o C for 72 h in Brain Heart Infusion (BHI) agar. The count present reduction of one logarithmic cycle in the number of colonies in the 5 kGy irradiated honeys. In the samples irradiated with doses of 10 and 15 kGy there was no microbial growth and spore germination. It’s concluded that irradiation constitutes an excellent conservation method, which can be used to guarantee the microbiological quality of the honey.

Cultivation and Propagation of Iris laevigata Fisch., an Endangered Ethno-medicinal Plant of Imphal Valley Manipur, India

Iris laevigata Fisch., is restricted geographically in Manipur, north eastern India. An experiment was planned with Factorial Randomized Block Design during 2018 to 2020 (3 seasons) in the study plots of ICAR Research Complex for NEH Region, Manipur Centre, Lamphelpat. The study encompasses on the growth and development of I. laevigata seedlings collected from Ipa Thoukok Complex: influenced by spacing, trimming and correlations amongst the growth parameters, so as to validate the most favourable conservation method for this endangered plant. Among the treatments, T6 = S2T3 (45 cm × trimming 40 DAT) was found the best treatment. T9 = S3T3 (60 cm × trimming 40 DAT) was second, T3 = S1T3 (30 cm × trimming 40 DAT) was the third and lowest was found in case of T1 = S1T1 (30 cm × 0 trimming, i.e., without trimming). Strong positive correlation between leaf surface area and plant height on the growth and development was found to be effective among other correlations.

Multivariate Modelling of Milk Fatty Acid Profile to Discriminate the Forages in Dairy Cows' Ration

Although there are many studies on the importance of fatty acids (FA) in our diet and on the influence of cows’ diet on FA metabolism, only a few investigate their predictive capacity to discriminate the type, amount and conservation method of the dietary forages. This research quantitatifies differences in FA concentrations and, using a supervised factorial discriminant analysis (FDA), assesses which could be biomarkers when replacing maize silage with other silages, grass/lucerne hays or fresh grass. The statistical modelling identified three main clusters in milk FA profiles associated with silages, hays and fresh grass as dominant roughages. The main implication of a dairy cow feeding system based on poliphytic forages from permanent meadows is to enhance milk’s nutritional value thanks to an increase in beneficial FA belonging to omenga-3 polyunsaturated FA, conjugated linoleic acids and odd chain FA, compared to the use of maize silage from arable land. The study also identified a small but powerful and reliable pool of FA that can act as biomarkers to authenticate feeding systems: C16:1, C17:0, C18:0, C18:3n-3, C18:1 c-9, C18:1 t-11, C20:0.