Commercialization of veterinary viral vaccines

2004 ◽  
Vol 5 (2) ◽  
pp. 239-242 ◽  
Author(s):  
P. H. Flore
Keyword(s):  
Large Scale ◽  
Raw Materials ◽  
Scale Up ◽  
Cost Effective ◽  
Production Environment ◽  
Considerable Effort ◽  
Veterinary Vaccine ◽  
Viral Vaccine ◽  
Development Department

AbstractIf vaccines are to reliably prevent disease, they must be developed, produced and quality-controlled according to very strict regulations and procedures. Veterinary viral vaccine registrations are governed by different rules in different countries, but these rules all emphasize that the quality of the raw materials—the cells, eggs, animals or plants that are used in production—need to be carefully controlled. The veterinary vaccine business is also very cost-conscious. Emphasis over the last 5–10 years has therefore been to develop culture systems that minimize labor and sterility problems and thus provide for reliable and cost-effective production. Implementing these often more complex systems in a production environment takes considerable effort, first in scale-up trials and further down the line in convincing production personnel to change their familiar system for something new and possibly untried. To complete scale-up trials successfully, it is absolutely necessary to understand the biochemistry of the cells and the influence of the virus on the cells under scale-up and later production conditions. Once a viral product can be produced on a large scale, it is imperative that the quality of the end-product is controlled in an intelligent way. One needs to know whether the end-product performs in the animal as was intended during its conception in the research and development department. The development of the appropriate tests to demonstrate this plays an important role in the successful development of a vaccine.

scholarly journals Modeling Fused Filament Fabrication using Artificial Neural Networks

2021 ◽  
Author(s):  
Paul Oehlmann ◽  
Paul Osswald ◽  
Juan Camilo Blanco ◽  
Martin Friedrich ◽  
Dominik Rietzel ◽  
...  
Keyword(s):  
Real Time ◽  
Large Scale ◽  
Cost Effective ◽  
Print Quality ◽  
Ann Model ◽  
Production Environment ◽  
Fused Filament Fabrication ◽  
Artificial Neural ◽  
Using Data ◽  
Artificial Neural Network Ann

AbstractWith industries pushing towards digitalized production, adaption to expectations and increasing requirements for modern applications, has brought additive manufacturing (AM) to the forefront of Industry 4.0. In fact, AM is a main accelerator for digital production with its possibilities in structural design, such as topology optimization, production flexibility, customization, product development, to name a few. Fused Filament Fabrication (FFF) is a widespread and practical tool for rapid prototyping that also demonstrates the importance of AM technologies through its accessibility to the general public by creating cost effective desktop solutions. An increasing integration of systems in an intelligent production environment also enables the generation of large-scale data to be used for process monitoring and process control. Deep learning as a form of artificial intelligence (AI) and more specifically, a method of machine learning (ML) is ideal for handling big data. This study uses a trained artificial neural network (ANN) model as a digital shadow to predict the force within the nozzle of an FFF printer using filament speed and nozzle temperatures as input data. After the ANN model was tested using data from a theoretical model it was implemented to predict the behavior using real-time printer data. For this purpose, an FFF printer was equipped with sensors that collect real time printer data during the printing process. The ANN model reflected the kinematics of melting and flow predicted by models currently available for various speeds of printing. The model allows for a deeper understanding of the influencing process parameters which ultimately results in the determination of the optimum combination of process speed and print quality.

scholarly journals Direct detection of SARS-CoV-2 using non-commercial RT-LAMP reagents on heat-inactivated samples

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alisa Alekseenko ◽  
Donal Barrett ◽  
Yerma Pareja-Sanchez ◽  
Rebecca J. Howard ◽  
Emilia Strandback ◽  
...  
Keyword(s):  
Large Scale ◽  
Direct Detection ◽  
Dna Polymerases ◽  
Scale Up ◽  
Lamp Assay ◽  
Cost Effective ◽  
Reaction Conditions ◽  
Clinical Patient ◽  
Reverse Transcriptases ◽  
Large Scale Testing

AbstractRT-LAMP detection of SARS-CoV-2 has been shown to be a valuable approach to scale up COVID-19 diagnostics and thus contribute to limiting the spread of the disease. Here we present the optimization of highly cost-effective in-house produced enzymes, and we benchmark their performance against commercial alternatives. We explore the compatibility between multiple DNA polymerases with high strand-displacement activity and thermostable reverse transcriptases required for RT-LAMP. We optimize reaction conditions and demonstrate their applicability using both synthetic RNA and clinical patient samples. Finally, we validate the optimized RT-LAMP assay for the detection of SARS-CoV-2 in unextracted heat-inactivated nasopharyngeal samples from 184 patients. We anticipate that optimized and affordable reagents for RT-LAMP will facilitate the expansion of SARS-CoV-2 testing globally, especially in sites and settings where the need for large scale testing cannot be met by commercial alternatives.

Biological Synthesis of Silver Nanoparticles and their Biomedical Activity: A Review

2021 ◽  
Vol 09 ◽  
Author(s):  
Sarvat Zafar ◽  
Aiman Zafar ◽  
Fakhra Jabeen ◽  
Miad Ali Siddiq
Keyword(s):  
Silver Nanoparticles ◽  
Large Scale ◽  
Scale Up ◽  
Cost Effective ◽  
Biological Properties ◽  
Single Step ◽  
Biological Synthesis ◽  
Nanosized Particles ◽  
Environment Friendly ◽  
Physical And Chemical

: Nanotechnology studies the various phenomena of physio-chemical procedures and biological properties for the generation of nanosized particles, and their rising challenges in the various sectors, like medicine, engineering, agriculture, electronic, and environmental studies. The nanosized particles exhibit good anti-microbial, anti-inflammatory, cytotoxic, drug delivery, anti-parasitic, anti-coagulant and catalytic properties because of their unique dimensions with large surface area, chemical stability and higher binding density for the accumulation of various bio-constituents on their surfaces. Biological approaches for the synthesis of silver nanoparticles (AgNPs) have been reviewed because it is an easy and single-step protocol and a viable substitute for the synthetic chemical-based procedures. Physical and chemical approaches for the production of AgNPs are also mentioned herein. Biological synthesis has drawn attention because it is cost-effective, faster, non-pathogenic, environment-friendly, easy to scale-up for large-scale synthesis, and having no demand for usage of high pressure, energy, temperature, or noxious chemical ingredients, and safe for human therapeutic use. Therefore, the collaboration of nanomaterials with bio-green approaches could extend the utilization of biological and cytological properties compatible with AgNPs. In this perspective, there is an immediate need to develop ecofriendly and biocompatible techniques, which strengthen efficacy against microbes and minimize toxicity for human cells. The present study introduces the biological synthesis of silver nanoparticles, and their potential biomedical applications have also been reviewed.

Identification and Detection of Undeclared Herbal Slimming Adulterants

2016 ◽  
Vol 2 (1 and 2) ◽  
pp. 73-78
Author(s):  
Mohammad Kamil
Keyword(s):  
Public Health ◽  
Raw Materials ◽  
Health And Safety ◽  
Herbal Medicines ◽  
Cost Effective ◽  
Herbal Drugs ◽  
Visual Evaluation ◽  
Synthetic Drugs ◽  
Artery Disease

The Food and Drug Administration (FDA) is advising consumers to stop using multiple weight-loss products that contain the undeclared drug ingredients e.g. sibutramine, which was removed from the market in 2010 for safety reasons and may present significant risks for those with coronary artery disease and other heart issues. Sibutramine and similar undeclared ingredients in herbal medicines are a real challenge for the public health and safety. In recent years, the need for quality assurance tools to ensure the identity, purity, and quality of botanical material has risen dramatically. HPTLC has emerged as a versatile, high throughput, and cost-effective technology, that is uniquely suited to meet these requirements. Most separation techniques do not allow parallel analysis of numerous samples at the same time and they often face problems in separating complex mixtures of substances. However, visual evaluation of HPTLC plates allows for convenient comparison of many samples side by side, where similarities and differences can clearly be seen. The quality of raw materials is rapidly and easily determined by HPTLC. For the identification of herbal drugs and other naturally derived materials standardized HPTLC is the method of choice and recommended by pharmacopoeias worldwide, furthermore adulterated samples are reliably identified. The main objective of the present study is to check pharmaceutical analogue adulteration of nonprescription and prescription slimming products in the laboratory using chromatographic techniques and to discuss its side effects in the interest of consumers and public health safety. Since intentional adulteration of “natural herbalmedicines” with unknown synthetic drugs or chemicals is a common and dangerous phenomenon of alternative medicine, it is important to modify and validate analytical tools to monitor and evaluate these herbal drugs.

Direct Synthesis of MoSe2 Thin Films on SiO2/Si Using Selenization Process of Sputtered Molybdenum

2020 ◽  
Vol 15 (5) ◽  
pp. 580-585
Author(s):  
Dae-Young Um ◽  
R. Nandi ◽  
Jeong-Hun Yang ◽  
Jin-Soo Kim ◽  
Jong-Woong Kim ◽  
...  
Keyword(s):  
Large Scale ◽  
Direct Synthesis ◽  
Cost Effective ◽  
Metal Films ◽  
Crystalline Quality ◽  
Research Attention ◽  
Molybdenum Diselenide ◽  
Potential Applications ◽  
Direct Current Sputtering

Recently, molybdenum diselenide (MoSe2) has attracted nascent research attention for potential applications in electronic and optoelectronic devices due to its unique properties including tunable bandgap, strong photoluminescence and large exciton binding energy. However, the synthesis of reproducible, controlled and large scale MoSe2 films is still a great challenge. Here, we have investigated the morphology, structure and crystalline quality of MoSe2 films synthesized by the selenization of Mo metal films. The Mo metal films of different thicknesses were deposited at room temperature by direct current sputtering. Subsequently, MoSe2 films were prepared by selenization of sputtered Mo films at 550 °C for 20 minutes. The obtained MoSe2 films are polycrystalline with hexagonal crystal structure. The crystalline quality of the MoSe2 films is improved with increase in the thickness of Mo metal films. The MoSe2 films are found to be n-type in nature and reasonably stoichiometric (Mo/Se ratio ∼1:1.9). This study provides an experimental demonstration of an alternative cost-effective direct synthesis of MoSe2 films on SiO2/Si for the applications of semiconductor devices.

scholarly journals An Improved In Vitro Pyrogen Test To Detect The Presence of Endotoxin Containing Bacteria Using Limulus Amoebocyte Lysate Assay From Pharmaceutical Raw Product

1970 ◽  
Vol 1 (1) ◽  
pp. 76-79
Author(s):  
Tanvir Bashar ◽  
Khondokar Mahzebin Shurovi ◽  
Sanjida Dilshad
Keyword(s):  
Pathogenic Bacteria ◽  
Raw Materials ◽  
Cost Effective ◽  
Drug Preparation ◽  
Effective Manner ◽  
Key Word ◽  
Pseudomonas Species ◽  
Lal Assay

Most of the environmental organism possess endotoxin which presence indicates the organisms are much lethal and for the purposes bacteriological quality of pharmaceutical raw products is much important. In these current study, 2 raw materials out of 10 were found to containing pathogenic bacteria Escherichia coli and Pseudomonas species in the culture medium, that indicates the raw materials were contaminated with the deadly organism. These two raw materials checked for the presence of Endotoxin and both provided positive gel clot by Limulas amoebocyte lysate (LAL) assay. Quality maintenance and assurance is the essential need of Drug preparation in pharmaceutical sector. The result ensure that Pharmaceutical industry should need to follow GMP and HACCP to minimize the contamination for improving the biological safety of the product in a cost-effective manner. Key Word: Limulas amoebocyte lysate (LAL) assay, Gram negative bacteria, endotoxin. doi:10.3329/sjps.v1i1.1812    S. J. Pharm. Sci. 1(1&2): 76-79

GROUP COGNITIVE THERAPY: TREATMENT OF CHOICE OR SUB-OPTIMAL OPTION?

2001 ◽  
Vol 29 (3) ◽  
pp. 311-332 ◽  
Author(s):  
Norma Morrison
Keyword(s):  
Group Therapy ◽  
Cognitive Therapy ◽  
Large Scale ◽  
Cost Effective ◽  
Advantages And Disadvantages ◽  
Quality Of Therapy ◽  
Group Format ◽  
Cognitive Behavioural ◽  
Therapy Treatment

In the present climate of limited resources and long waiting lists, it is not surprising that there is more emphasis on making sure that psychological treatments are not only clinically sound but also cost-effective. One solution to this is to provide time-limited, focused interventions such as cognitive therapy. Another obvious solution is to deliver treatment in groups rather than individually. However, what evidence is there that therapy can be delivered as effectively in groups as individually? This review will look at which different formats have been tried, what the advantages and disadvantages of those formats might be, which client groups have been targeted for cognitive- behavioural group therapy (CBGT), and whether a group format in general offers any advantages over individual CBT. Outcome studies and their implications for the use of CBGT are considered. Results suggest that, in most client groups, there is little difference in efficacy between group and individual CBT, although there is some evidence that results for some types of patient can be disappointing in CBGT. It may be that the best compromise in terms of cost- effectiveness between quality of therapy and quantity of patients treated is offered by large-scale psychoeducational didactic group therapy.

scholarly journals Upstream to downstream: a multiple-assessment-point approach for targeting non-point-source priority management areas at large watershed scale

2013 ◽  
Vol 10 (11) ◽  
pp. 14535-14555
Author(s):  
L. Chen ◽  
Y. Zhong ◽  
G. Wei ◽  
Z. Shen
Keyword(s):  
Point Source ◽  
Large Scale ◽  
Cost Effective ◽  
Small Scale ◽  
Watershed Scale ◽  
Nps Pollution ◽  
Specific Assessment ◽  
High Level ◽  
Multiple Assessment

Abstract. The identification of priority management areas (PMAs) is essential for the control of non-point source (NPS) pollution, especially for a large-scale watershed. However, previous studies have typically focused on small-scale catchments adjacent to specific assessment points; thus, the interactions between multiple river points remain poorly understood. In this study, a multiple-assessment-point PMA (MAP-PMA) framework was proposed by integrating the upstream sources and the downstream transport aspects of NPS pollution. Based on the results, the integration of the upstream input changes was vital for the final PMAs map, especially for downstream areas. Contrary to conventional wisdom, this research recommended that the NPS pollutants could be best controlled among the upstream high-level PMAs when protecting the water quality of the entire watershed. The MAP-PMA framework provided a more cost-effective tool for the establishment of conservation practices, especially for a large-scale watershed.

scholarly journals Features of changes in the ecological situation in the Russian Federation and mechanisms for supporting the development of small towns

2017 ◽  
Vol 9 (1) ◽  
pp. 49-64
Author(s):  
Olga Malikova
Keyword(s):  
Russian Federation ◽  
Large Scale ◽  
Raw Materials ◽  
Small Towns ◽  
Ecological Situation ◽  
Urban Agglomerations ◽  
Large Cities ◽  
The Russian Federation ◽  
The Impact

The formation of large urban agglomerations makes possible to ensure more rational use of productive resources and increase labor productivity. Large cities, as a rule, have developed infrastructure and educational sector. However, there is a difficult ecological situation in many large cities. With a large scale of the city, transport accessibility is seriously complicated. From the viewpoint of favorable habitat, good ecological conditions, small and medium-sized cities often benefit from urban agglomerations. In the Russian Federation, due to the dominance of raw materials industries and the underdevelopment of local industry, small and medium-sized cities are in decline. This predetermines the need to develop special measures to support such territorial entities. The study is based on the generalization of data on the change in the ecological situation in large cities and the impact of atmospheric air pollution on the quality of life of the population. In the framework of the study, the conclusion was substantiated about the expediency of carrying out special measures aimed at improving the ecological situation in large cities and developing measures to support small and medium-sized cities. The recommendations for stimulating the development of production in small and medium-sized cities are grounded. The results of the study can find applications in the practice of regional management.

scholarly journals RAPID AND COST-EFFECTIVE PROCESS BASED ON INSECT LARVAE FOR SCALE-UP PRODUCTION OF SARS-COV-2 SPIKE PROTEIN FOR SEROLOGICAL COVID-19 TESTING

2021 ◽  
Author(s):  
Ignacio Smith ◽  
Gregorio Mc Callum ◽  
Adriana Sabljic ◽  
Juan Marfia ◽  
Silvina Bombicino ◽  
...  
Keyword(s):  
Large Scale ◽  
Scale Up ◽  
High Sensitivity ◽  
Cost Effective ◽  
Elisa Method ◽  
Insect Larvae ◽  
S Protein ◽  
Single Antigen ◽  
Antibody Kinetics ◽  
Virus Strains

Serology testing for COVID-19 is important in evaluating active immune response against SARS-CoV-2, studying the antibody kinetics, and monitoring reinfections with genetic variants and new virus strains, in particular, the duration of antibodies in virus-exposed individuals and vaccine-mediated immunity. In this work, recombinant S protein of SARS-CoV-2 was expressed in Rachiplusia nu, an important agronomic plague. One larva produces an amount of S protein sufficient for 150 determinations in the ELISA method herein developed. We established a rapid production process for SARS-CoV-2 S protein that showed immunoreactivity for anti-SARS-CoV-2 antibodies and was used as a single antigen for developing the ELISA method with high sensitivity (96.2%) and specificity (98.8%). Our findings provide an efficient and cost-effective platform for large-scale S protein production, and the scale-up is linear, thus avoiding the use of complex equipment like bioreactors.

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