scholarly journals Identification and Characterization of a New Class of Trafficking Motifs for Controlling Clathrin-independent Internalization and Recycling

2007 ◽  
Vol 282 (17) ◽  
pp. 13087-13097 ◽  
Author(s):  
Qiang Gong ◽  
Michael Weide ◽  
Christopher Huntsman ◽  
Zhuojin Xu ◽  
Lily Y. Jan ◽  
...  
Keyword(s):  
New Class ◽  
Identification And Characterization

scholarly journals Identification and Characterization of Circular RNAs as a New Class of Putative Biomarkers in Diabetes Retinopathy

2017 ◽  
Vol 58 (14) ◽  
pp. 6500 ◽  
Author(s):  
Shu-Jie Zhang ◽  
Xue Chen ◽  
Chao-Peng Li ◽  
Xiu-Miao Li ◽  
Chang Liu ◽  
...  
Keyword(s):  
Circular Rnas ◽  
New Class ◽  
Diabetes Retinopathy ◽  
Identification And Characterization

scholarly journals A mycobacterial glycine-rich protein governs actin-based motility

2021 ◽  
Author(s):  
Norbert S. Hill ◽  
Matthew D. Welch
Keyword(s):  
Lipid Droplet ◽  
Actin Polymerization ◽  
Close Relative ◽  
Bacterial Surface ◽  
Motility Factor ◽  
New Class ◽  
Intracellular Movement ◽  
Mycobacterial Pathogenesis ◽  
Identification And Characterization

Mycobacterium marinum, a close relative of the significant human pathogen Mycobacterium tuberculosis, polymerizes host actin at the bacterial surface to drive intracellular movement and cell-to-cell spread during infection. Here, we report the identification and characterization of MirA, the M. marinum actin-based motility factor. MirA is a member of the glycine-rich PE_PGRS family of ESX-5-secreted proteins. MirA uses an amphipathic helix to anchor into the mycobacterial outer membrane and, surprisingly, also the surface of host lipid droplet organelles. The glycine-rich PGRS domain in MirA directly binds and activates host N-WASP to stimulate actin polymerization through the Arp2/3 complex, directing both bacterial and lipid droplet actin-based motility. MirA is dissimilar to known N-WASP activating ligands and may represent a new class of microbial and host actin regulator. Additionally, the MirA-N-WASP interaction represents a model to understand how the enigmatic PE_PGRS proteins contribute to mycobacterial pathogenesis.

Identification and Characterization of a New Class of (6–4) Photolyase from Vibrio cholerae

Biochemistry ◽  
2019 ◽  
Vol 58 (43) ◽  
pp. 4352-4360 ◽  
Author(s):  
Ugur Meric Dikbas ◽  
Mehmet Tardu ◽  
Asena Canturk ◽  
Seref Gul ◽  
Gozde Ozcelik ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
New Class ◽  
Identification And Characterization

scholarly journals Long read sequencing reveals a novel class of structural aberrations in cancers: identification and characterization of cancerous local amplifications

2019 ◽  
Author(s):  
Yoshitaka Sakamoto ◽  
Liu Xu ◽  
Masahide Seki ◽  
Toshiyuki T. Yokoyama ◽  
Masahiro Kasahara ◽  
...  
Keyword(s):  
Point Mutations ◽  
Lung Cancers ◽  
New Class ◽  
Large Deletions ◽  
Long Read ◽  
Structural Aberrations ◽  
Molecular Etiology ◽  
Identification And Characterization

AbstractHere we report identification of a new class of local structural aberrations in lung cancers. The whole-genome sequencing of cell lines using a long read sequencer, PromethION, demonstrated that typical cancerous mutations, such as point mutations, large deletions and gene fusions can be detected also on this platform. Unexpectedly, we revealed unique structural aberrations consisting of complex combinations of local duplications, inversions and micro deletions. We further analyzed and found that these mutations also occur in vivo, even in key cancer-related genes. These mutations may elucidate the molecular etiology of patients for whom causative cancerous events and therapeutic strategies remain elusive.

scholarly journals Identification and Characterization of Circular RNAs As a New Class of Putative Biomarkers in Human Blood

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0141214 ◽  
Author(s):  
Sebastian Memczak ◽  
Panagiotis Papavasileiou ◽  
Oliver Peters ◽  
Nikolaus Rajewsky
Keyword(s):  
Human Blood ◽  
Circular Rnas ◽  
New Class ◽  
Identification And Characterization

scholarly journals Identification and Characterization of a New Class of Bilin Lyase

2006 ◽  
Vol 281 (26) ◽  
pp. 17768-17778 ◽  
Author(s):  
Gaozhong Shen ◽  
Nicolle A. Saunée ◽  
Shervonda R. Williams ◽  
Eduardo F. Gallo ◽  
Wendy M. Schluchter ◽  
...  
Keyword(s):  
New Class ◽  
Identification And Characterization

scholarly journals Identification and characterization of influenza variants resistant to a viral endonuclease inhibitor

2016 ◽  
Vol 113 (13) ◽  
pp. 3669-3674 ◽  
Author(s):  
Min-Suk Song ◽  
Gyanendra Kumar ◽  
William R. Shadrick ◽  
Wei Zhou ◽  
Trushar Jeevan ◽  
...  
Keyword(s):  
Point Mutations ◽  
Random Mutagenesis ◽  
Resistant Mutant ◽  
Inhibitor Binding ◽  
New Class ◽  
Identification And Characterization ◽  
Modest Reduction

The influenza endonuclease is an essential subdomain of the viral RNA polymerase. It processes host pre-mRNAs to serve as primers for viral mRNA and is an attractive target for antiinfluenza drug discovery. Compound L-742,001 is a prototypical endonuclease inhibitor, and we found that repeated passaging of influenza virus in the presence of this drug did not lead to the development of resistant mutant strains. Reduced sensitivity to L-742,001 could only be induced by creating point mutations via a random mutagenesis strategy. These mutations mapped to the endonuclease active site where they can directly impact inhibitor binding. Engineered viruses containing the mutations showed resistance to L-742,001 both in vitro and in vivo, with only a modest reduction in fitness. Introduction of the mutations into a second virus also increased its resistance to the inhibitor. Using the isolated wild-type and mutant endonuclease domains, we used kinetics, inhibitor binding and crystallography to characterize how the two most significant mutations elicit resistance to L-742,001. These studies lay the foundation for the development of a new class of influenza therapeutics with reduced potential for the development of clinical endonuclease inhibitor-resistant influenza strains.

scholarly journals Identification and Characterization of FTY720 for the Treatment of Human African Trypanosomiasis

2015 ◽  
Vol 60 (3) ◽  
pp. 1859-1861 ◽  
Author(s):  
Amy J. Jones ◽  
Marcel Kaiser ◽  
Vicky M. Avery
Keyword(s):  
Human African Trypanosomiasis ◽  
Oral Drug ◽  
African Trypanosomiasis ◽  
Content Type ◽  
New Class ◽  
Trypanosoma Brucei Gambiense ◽  
Causative Agents ◽  
Identification And Characterization ◽  
Relapsing Multiple Sclerosis

The screening of a focused library identified FTY720 (Fingolimod; Gilenya) as a potent selective antitrypanosomal compound active againstTrypanosoma brucei gambienseandT. brucei rhodesiense, the causative agents of human African trypanosomiasis (HAT). This is the first report of trypanocidal activity for FTY720, an oral drug registered for the treatment of relapsing multiple sclerosis, and the characterization of sphingolipids as a potential new class of compounds for HAT.

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