scholarly journals Identification and characterization of a new class of Tomato spotted wilt virus isolates that break Tsw ‐based resistance in a temperature‐dependent manner

2018 ◽  
Vol 68 (1) ◽  
pp. 60-71 ◽  
Author(s):  
D. Ronde ◽  
D. Lohuis ◽  
R. Kormelink
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Dependent Manner ◽  
Temperature Dependent ◽  
New Class ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Identification And Characterization ◽  
Virus Isolates

scholarly journals Identification and characterization of host proteins interacting with NSm, the Tomato spotted wilt virus movement protein

2002 ◽  
Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽  
pp. S108-S111
Author(s):  
M. Paape ◽  
S. Nell ◽  
S. von Bargen ◽  
J.-W. Kellmann
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Movement Protein ◽  
Host Proteins ◽  
Tomato Spotted Wilt ◽  
J Domain ◽  
Wilt Virus ◽  
Identification And Characterization ◽  
Two Hybrid ◽  
Interaction Trap

To search for host proteins involved in systemic spreading of Tomato spotted wilt virus (TSWV), the virus-encoded NSm movement protein has been utilized as a bait in yeast two-hybrid interaction trap assays. J-domain chaperones from different host species and a protein denominated At-4/1 from Arabidopsis thaliana showing homologies to myosins and kinesins were identified as NSm-interacting partners. In this communication we illustrate that following TSWV infection, J-domain proteins accumulated in systemically infected leaves of A. thaliana, whereas At-4/1 was constitutively detected in leaves of A. thaliana and Nicotiana rustica.

scholarly journals Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates

2012 ◽  
Vol 55 (04) ◽  
pp. 337-347 ◽  
Author(s):  
I. STANKOVIĆ ◽  
A. BULAJIĆ ◽  
A. VUČUROVIĆ ◽  
D. RISTIĆ ◽  
K. MILOJEVIĆ ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Tomato Spotted Wilt Virus ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Virus Isolates

scholarly journals Characterization of a New Tomato Spotted Wilt Virus Isolates Found in Hippeastrum hybridum (Hort.) Plants in Poland

2016 ◽  
Vol 24 (1) ◽  
pp. 5-12 ◽  
Author(s):  
Hanna Berniak
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Sweet Pepper ◽  
Mild Mosaic ◽  
Nucleotide Sequence Level ◽  
Tomato Spotted Wilt ◽  
Genetic Patterns ◽  
Necrotic Spots ◽  
Wilt Virus ◽  
Virus Isolates

Abstract Two Tomato spotted wilt virus (TSWV) isolates H1 and H2 found in Hippeastrum hybridum plants were characterized based on biological, serological, and molecular properties. Virus isolates showed differences in symptom expression – H1 isolate displayed severe necrotic spots and patterns, whereas mild mosaic symptoms were observed on H2-infected H. hybridum plants. Both TSWV isolates showed comparable reactivity with TSWV-specific antibodies and they induced similar symptoms on herbaceous indicator plants, but some differences between these isolates were detected at the nucleotide sequence level of genomic S and M ssRNAs segment fragments. The nucleotide sequences encoding nucleocapsid (N) and nonstructural (NSs and NSm) proteins showed 98.2%, 97.5%, and 96.5% identity, respectively. Phylogenetic analysis of N and NSs sequences conducted for tested isolates and 31 TSWV isolates included for comparison revealed that H1 and H2 isolates fell into the same cluster and they were grouped together with isolates found previously in different vegetables, ornamentals, and weeds. When NSm ORF was analyzed, the tested isolates formed a separate cluster: H1 isolate showed the highest affinity with TSWV isolates infecting chrysanthemum and pepper plants, whereas H2 isolate was most closely related to other virus isolates found in sweet pepper and tomatoes. These results indicate that both isolates were reassortants between different virus isolates, and represented two novel genetic patterns of TSWV.

scholarly journals Characterization of a vector-non-transmissible isolate of Tomato spotted wilt virus

2007 ◽  
Vol 0 (0) ◽  
pp. 070928213247001-??? ◽  
Author(s):  
R. A. Naidu ◽  
J. L. Sherwood ◽  
C. M. Deom
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

scholarly journals Tomato Spotted Wilt Virus Isolates in Japan are Grouped into Two Strains.

1993 ◽  
Vol 59 (6) ◽  
pp. 626-634 ◽  
Author(s):  
Shinya TSUDA ◽  
Kaoru HANADA ◽  
Yuzo MINOBE ◽  
Mitsuro KAMEYA-IWAKI ◽  
Keiichi TOMARU
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Virus Isolates

Biological and molecular characterization of tomato spotted wilt virus (TSWV) infecting Chrysanthemum in India

2020 ◽  
Author(s):  
Chinnaiah Senthilraja ◽  
Varagur Ganesan Malathi ◽  
Sevugapperumal Nakkeeran ◽  
Mariappan Suganthy ◽  
Uthandi Sivakumar ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Tomato Spotted Wilt Virus ◽  
Tomato Spotted Wilt ◽  
Wilt Virus
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