scholarly journals Insulin-stimulated Hydrogen Peroxide Reversibly Inhibits Protein-tyrosine Phosphatase 1Bin Vivoand Enhances the Early Insulin Action Cascade

2001 ◽  
Vol 276 (24) ◽  
pp. 21938-21942 ◽  
Author(s):  
Kalyankar Mahadev ◽  
Assaf Zilbering ◽  
Li Zhu ◽  
Barry J. Goldstein
Keyword(s):  
Hydrogen Peroxide ◽  
Protein Tyrosine Phosphatase ◽  
Insulin Action ◽  
Tyrosine Phosphatase ◽  
Protein Tyrosine

scholarly journals Inhibition of the Protein Tyrosine Phosphatase SHP-1 Increases Glucose Uptake in Skeletal Muscle Cells by Augmenting Insulin Receptor Signaling and GLUT4 Expression

Endocrinology ◽  
2011 ◽  
Vol 152 (12) ◽  
pp. 4581-4588 ◽  
Author(s):  
Sébastien Bergeron ◽  
Marie-Julie Dubois ◽  
Kerstin Bellmann ◽  
Michael Schwab ◽  
Nancy Larochelle ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protein Expression ◽  
Glucose Uptake ◽  
Protein Tyrosine Phosphatase ◽  
Insulin Action ◽  
Tyrosine Phosphatase ◽  
Glycogen Synthesis ◽  
Glut4 Translocation ◽  
Protein Tyrosine ◽  
Glut4 Expression

The protein tyrosine phosphatase (PTPase) Src-homology 2-domain-containing phosphatase (SHP)-1 was recently reported to be a novel regulator of insulin's metabolic action. In order to examine the role of this PTPase in skeletal muscle, we used adenovirus (AdV)-mediated gene transfer to express an interfering mutant of SHP-1 [dominant negative (DN)SHP-1; mutation C453S] in L6 myocytes. Expression of DNSHP-1 increased insulin-induced Akt serine-threonine kinase phosphorylation and augmented glucose uptake and glycogen synthesis. Pharmacological inhibition of glucose transporter type 4 (GLUT4) activity using indinavir and GLUT4 translocation assays revealed an important role for this transporter in the increased insulin-induced glucose uptake in DNSHP-1-expressing myocytes. Both GLUT4 mRNA and protein expression were also found to be increased by DNSHP-1 expression. Furthermore, AdV-mediated delivery of DNSHP-1 in skeletal muscle of transgenic mice overexpressing Coxsackie and AdV receptor also enhanced GLUT4 protein expression. Together, these findings confirm that SHP-1 regulates muscle insulin action in a cell-autonomous manner and further suggest that the PTPase negatively modulates insulin action through down-regulation of both insulin signaling to Akt and GLUT4 translocation, as well as GLUT4 expression.

scholarly journals Developmental Switch from Prolonged Insulin Action to Increased Insulin Sensitivity in Protein Tyrosine Phosphatase 1B-Deficient Hepatocytes

Endocrinology ◽  
2007 ◽  
Vol 148 (2) ◽  
pp. 594-608 ◽  
Author(s):  
Agueda Gonzalez-Rodriguez ◽  
Jill E. Clampit ◽  
Oscar Escribano ◽  
Manuel Benito ◽  
Cristina M. Rondinone ◽  
...  
Keyword(s):  
Insulin Sensitivity ◽  
Protein Tyrosine Phosphatase ◽  
Insulin Action ◽  
Tyrosine Phosphatase ◽  
Protein Tyrosine Phosphatase 1B ◽  
Protein Tyrosine ◽  
Developmental Switch

Small Molecule Peptidomimetics Containing a Novel Phosphotyrosine Bioisostere Inhibit Protein Tyrosine Phosphatase 1B and Augment Insulin Action‡

Biochemistry ◽  
2001 ◽  
Vol 40 (19) ◽  
pp. 5642-5654 ◽  
Author(s):  
John E. Bleasdale ◽  
Derek Ogg ◽  
Barbara J. Palazuk ◽  
Cynthia S. Jacob ◽  
Michael L. Swanson ◽  
...  
Keyword(s):  
Small Molecule ◽  
Protein Tyrosine Phosphatase ◽  
Insulin Action ◽  
Tyrosine Phosphatase ◽  
Protein Tyrosine Phosphatase 1B ◽  
Protein Tyrosine

scholarly journals Activation of Hydrogen Peroxide to Peroxytetradecanoic Acid Is Responsible for Potent Inhibition of Protein Tyrosine Phosphatase CD45

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e52495 ◽  
Author(s):  
Alicja Kuban-Jankowska ◽  
Jack A. Tuszynski ◽  
Philip Winter ◽  
Magdalena Gorska ◽  
Narcyz Knap ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Protein Tyrosine Phosphatase ◽  
Tyrosine Phosphatase ◽  
Protein Tyrosine ◽  
Potent Inhibition ◽  
Activation Of Hydrogen Peroxide
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