Isolation and characterisation of 16 novel polymorphic microsatellite loci for the Eurasian coot (Fulica atra)

2016 ◽  
Vol 64 (6) ◽  
pp. 430
Author(s):  
Tianshu Lv ◽  
Wanchao Zhu ◽  
Shuyu Xue ◽  
Huanxin Zhang ◽  
Xiaodong Gao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Genomic Library ◽  
Enriched Genomic Library ◽  
Principal Coordinates Analysis ◽  
Principal Coordinates ◽  
Fulica Atra ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite ◽  
Two Populations

To study the genetic diversity of the Eurasian coot (Fulica atra), 16 novel microsatellites were isolated and characterised using a (AC)12-enriched genomic library. Polymorphism of each locus was detected in 19 individuals from Weishan Lake and Zhaoyang Lake, both located in Shandong Province, China. These microsatellite loci revealed 3–12 alleles in two populations. One locus (Fa6) showed significant deviations from Hardy–Weinberg equilibrium in the Zhaoyang Lake population. By means of Principal Coordinates Analysis, 19 individuals were divided into two groups, which verified they were from two populations. These novel microsatellites will be useful for further studies and genetic analyses of the Eurasian coot.

scholarly journals Isolation and Characterization of 10 Polymorphic Microsatellite Loci in Paphiopedilum concolor (Batem.) Pfitzer (Orchidaceae) and Cross-species Amplification

HortScience ◽  
2010 ◽  
Vol 45 (8) ◽  
pp. 1286-1287 ◽  
Author(s):  
Long-na Li ◽  
Songjun Zeng ◽  
Feng Zheng ◽  
Zhi-lin Chen ◽  
Kun-lin Wu ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Microsatellite Locus ◽  
Microsatellite Loci ◽  
Genomic Library ◽  
Enriched Genomic Library ◽  
Relationship Patterns ◽  
Isolation And Characterization ◽  
Expected Heterozygosity ◽  
Polymorphic Microsatellite

Ten polymorphic microsatellite loci were isolated and characterized from an enriched genomic library of Paphiopedilum concolor (Batem.) Pfitzer. The number of alleles per microsatellite locus ranged from three to 11 with an average of 6.4 in a sample of 30 individuals from three populations. The observed and expected heterozygosity ranged from 0.200 to 0.800 and from 0.544 to 0.827, respectively. These microsatellites can be used as tools to investigate the genetic structure of P. concolor populations and relationship patterns with closely related taxa.

scholarly journals Isolation and characterization of polymorphic microsatellite loci for Pistacia chinensis Bunge (Anacardiaceae)

2019 ◽  
Vol 68 (1) ◽  
pp. 26-28 ◽  
Author(s):  
Zhaohong Ding ◽  
Jianbo Lu
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Microsatellite Loci ◽  
Population Genetic ◽  
Magnetic Beads ◽  
Genomic Library ◽  
Landscape Fragmentation ◽  
Isolation And Characterization ◽  
Bioenergy Plant ◽  
Polymorphic Microsatellite

Abstract Pistacia chinensis Bunge (Anacardiaceae) is a native, dioecious, perennial arbor, and an important bioenergy plant. Twelve microsatellite markers were developed for P. chinensis to evaluate genetic diversity and population genetic structure when this species was subject to landscape fragmentation. Twelve polymorphic microsatellite loci were developed in P. chinensis using a microsatellite-enriched genomic library based on magnetic beads. These loci were characterized in 24 individuals from three populations located on Thousand Island Lake, Zhejiang Province, China. The number of alleles per locus varied from three to sixteen. The observed and expected heterozygosity ranged from 0.1250 to 0.8750 and 0.2333 to 0.8917, respectively. These microsatellite loci will be applied in further studies on the population genetic diversity and genetic structure of P. chinensis. This study will improve understanding of the effects of landscape fragmentation, and help conserve and manage the species.

scholarly journals Development of 11 Polymorphic Microsatellite Loci from Primula amthystina Franchet. (Primulaceae)

HortScience ◽  
2010 ◽  
Vol 45 (1) ◽  
pp. 148-149
Author(s):  
Yuan Huang ◽  
Xue-qin Wang ◽  
Chun-yan Yang ◽  
Chun-lin Long
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Loci ◽  
Southwest China ◽  
Herbaceous Plant ◽  
Genetic Population ◽  
Weinberg Equilibrium ◽  
Perennial Herbaceous Plant ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite

Primula amethystina Franchet. is a beautiful perennial herbaceous plant locally endemic to the alpine area in southwest China. We isolated and characterized 11 polymorphic microsatellite primer pairs from this species. The number of alleles ranged from two to five. The observed and expected heterozygosities (HO and HE) were 0.25 to 0.875 and 0.223 to 0.691, respectively. Six loci were significantly deviated from Hardy-Weinberg equilibrium as a result of the heterozygote deficiency. These markers will have great potential to reveal the genetic population structure and genetic diversity of P. amethystina.

scholarly journals Stand out from the Crowd: Small-Scale Genetic Structuring in the Endemic Sicilian Pond Turtle

Diversity ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 343
Author(s):  
Luca Vecchioni ◽  
Federico Marrone ◽  
Marco Arculeo ◽  
Uwe Fritz ◽  
Melita Vamberger
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Small Scale ◽  
Moderate Degree ◽  
Geographical Pattern ◽  
Genetic Structuring ◽  
Pond Turtle ◽  
Emys Trinacris ◽  
Polymorphic Microsatellite ◽  
Entire Distribution

The geographical pattern of genetic diversity was investigated in the endemic Sicilian pond turtle Emys trinacris across its entire distribution range, using 16 microsatellite loci. Overall, 245 specimens of E. trinacris were studied, showing high polymorphic microsatellite loci, with allele numbers ranging from 7 to 30. STRUCTURE and GENELAND analyses showed a noteworthy, geographically based structuring of the studied populations in five well-characterized clusters, supported by a moderate degree of genetic diversity (FST values between 0.075 and 0.160). Possible explanations for the genetic fragmentation observed are provided, where both natural and human-mediated habitat fragmentation of the Sicilian wetlands played a major role in this process. Finally, some conservation and management suggestions aimed at preventing the loss of genetic variability of the species are briefly reported, stressing the importance of considering the five detected clusters as independent Management Units.

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