scholarly journals Stand out from the Crowd: Small-Scale Genetic Structuring in the Endemic Sicilian Pond Turtle

Diversity ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 343
Author(s):  
Luca Vecchioni ◽  
Federico Marrone ◽  
Marco Arculeo ◽  
Uwe Fritz ◽  
Melita Vamberger
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Small Scale ◽  
Moderate Degree ◽  
Geographical Pattern ◽  
Genetic Structuring ◽  
Pond Turtle ◽  
Emys Trinacris ◽  
Polymorphic Microsatellite ◽  
Entire Distribution

The geographical pattern of genetic diversity was investigated in the endemic Sicilian pond turtle Emys trinacris across its entire distribution range, using 16 microsatellite loci. Overall, 245 specimens of E. trinacris were studied, showing high polymorphic microsatellite loci, with allele numbers ranging from 7 to 30. STRUCTURE and GENELAND analyses showed a noteworthy, geographically based structuring of the studied populations in five well-characterized clusters, supported by a moderate degree of genetic diversity (FST values between 0.075 and 0.160). Possible explanations for the genetic fragmentation observed are provided, where both natural and human-mediated habitat fragmentation of the Sicilian wetlands played a major role in this process. Finally, some conservation and management suggestions aimed at preventing the loss of genetic variability of the species are briefly reported, stressing the importance of considering the five detected clusters as independent Management Units.

scholarly journals First Insights into the Genetic Diversity of the Pinewood Nematode in Its Native Area Using New Polymorphic Microsatellite Loci

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e59165 ◽  
Author(s):  
Sophie Mallez ◽  
Chantal Castagnone ◽  
Margarida Espada ◽  
Paulo Vieira ◽  
Jonathan D. Eisenback ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Pinewood Nematode ◽  
Polymorphic Microsatellite

scholarly journals Development of microsatellite markers for the Japanese endemic conifer Thuja standishii and transfer to other East Asian species

2019 ◽  
Author(s):  
James Raymond Peter Worth ◽  
K. S. Chang ◽  
Y.-H. Ha ◽  
Aili Qin
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
East Asian ◽  
Clonal Reproduction ◽  
Sister Species ◽  
Asian Species ◽  
Expected Heterozygosity ◽  
Polymorphic Microsatellite ◽  
Diversity Structure

Abstract Objective: Design polymorphic microsatellite loci that will be useful for studies of the genetic diversity, structure and reproduction in the Japanese endemic conifer Thuja standishii and test the transferability of these loci to the two other East Asian species, T. sutchuenensis and T. koraiensis . Results: Fifteen loci were developed which displayed 3 to 21 alleles per locus (average = 9.2) among 97 samples from three populations of T. standishii . Observed heterozygosity for all samples varied between 0.33-0.75 (average 0.54) while expected heterozygosity values were higher with an average over the 15 loci of 0.62 (0.37-0.91). Low multi-locus probability of identity values (< 0.00001) indicate that these markers will be effective for identifying individuals derived from clonal reproduction. All 15 loci amplified in 13 samples of T. sutchuenensis , the sister species of T. standishii , with 1 to 11 alleles per locus (average = 4.33) while 13 loci amplified in four samples of the more distantly related T. koraiensis with 1 to 5 alleles per locus (average = 2.15).

scholarly journals Genetic Diversity of the Endemic Xantus’ Hummingbird Using 16 Novel Polymorphic Microsatellite Loci, and Their Cross Amplification between Six Related Species

2016 ◽  
Vol 06 (01) ◽  
pp. 19-27 ◽  
Author(s):  
Cristina González-Rubio Sanvicente ◽  
Ricardo Rodríguez-Estrella ◽  
Oscar Adrián Lozano-Garza ◽  
Francisco J. García-De-León
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Related Species ◽  
Cross Amplification ◽  
Polymorphic Microsatellite

Polymorphic microsatellite loci in the Chinese pond turtle (Chinemys reevesii)

2008 ◽  
Vol 10 (4) ◽  
pp. 1045-1048 ◽  
Author(s):  
Ronghui Ye ◽  
Rongquan Zheng ◽  
Lei Wang ◽  
Weiguo Du
Keyword(s):  
Microsatellite Loci ◽  
Pond Turtle ◽  
Polymorphic Microsatellite

scholarly journals Development of microsatellite markers for the Japanese endemic conifer Thuja standishii and transfer to other East Asian species

2019 ◽  
Author(s):  
James Raymond Peter Worth ◽  
K. S. Chang ◽  
Y.-H. Ha ◽  
Aili Qin
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
East Asian ◽  
Clonal Reproduction ◽  
Sister Species ◽  
Asian Species ◽  
Expected Heterozygosity ◽  
Polymorphic Microsatellite ◽  
Diversity Structure

Abstract Objective: Design polymorphic microsatellite loci that will be useful for studies of the genetic diversity, structure and reproduction in the Japanese endemic conifer Thuja standishii and test the transferability of these loci to the two other East Asian species, T. sutchuenensis and T. koraiensis . Results: Fifteen loci were developed which displayed 3 to 21 alleles per locus (average = 9.2) among 97 samples from three populations of T. standishii . Observed heterozygosity for all samples varied between 0.33-0.75 (average 0.54) while expected heterozygosity values were higher with an average over the 15 loci of 0.62 (0.37-0.91). Low multi-locus probability of identity values (< 0.00001) indicate that these markers will be effective for identifying individuals derived from clonal reproduction. All 15 loci amplified in 13 samples of T. sutchuenensis , the sister species of T. standishii , with 1 to 11 alleles per locus (average = 4.33) while 13 loci amplified in four samples of the more distantly related T. koraiensis with 1 to 5 alleles per locus (average = 2.15).

scholarly journals Development of microsatellite markers for the Japanese endemic conifer Thuja standishii and transfer to other East Asian species

2019 ◽  
Author(s):  
James Raymond Peter Worth ◽  
K. S. Chang ◽  
Y.-H. Ha ◽  
Aili Qin
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
East Asian ◽  
Clonal Reproduction ◽  
Sister Species ◽  
Asian Species ◽  
Expected Heterozygosity ◽  
Polymorphic Microsatellite ◽  
Diversity Structure

Abstract Objective Design polymorphic microsatellite loci that will be useful for studies of the genetic diversity, structure and reproduction in the Japanese endemic conifer Thuja standishii and test the transferability of these loci to the two other East Asian species, T. sutchuenensis and T. koraiensis. Results Fifteen loci were developed which displayed 3 to 21 alleles per locus (average = 9.2) among 97 samples from three populations of T. standishii . Observed heterozygosity for all samples varied between 0.33-0.75 (average 0.54) while expected heterozygosity values were higher with an average over the 15 loci of 0.62 (0.37-0.91). Low multi-locus probability of identity values (< 0.00001) indicate that these markers will be effective for identifying individuals derived from clonal reproduction. All 15 loci amplified in T. sutchuenensis, the sister species of T. standishii, with 1 to 11 alleles per locus (average = 4.33) while 13 loci amplified in the more distantly related T. koraiensis with 1 to 5 alleles per locus (average = 2.15).

scholarly journals Isolation and characterization of polymorphic microsatellite loci for Pistacia chinensis Bunge (Anacardiaceae)

2019 ◽  
Vol 68 (1) ◽  
pp. 26-28 ◽  
Author(s):  
Zhaohong Ding ◽  
Jianbo Lu
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Microsatellite Loci ◽  
Population Genetic ◽  
Magnetic Beads ◽  
Genomic Library ◽  
Landscape Fragmentation ◽  
Isolation And Characterization ◽  
Bioenergy Plant ◽  
Polymorphic Microsatellite

Abstract Pistacia chinensis Bunge (Anacardiaceae) is a native, dioecious, perennial arbor, and an important bioenergy plant. Twelve microsatellite markers were developed for P. chinensis to evaluate genetic diversity and population genetic structure when this species was subject to landscape fragmentation. Twelve polymorphic microsatellite loci were developed in P. chinensis using a microsatellite-enriched genomic library based on magnetic beads. These loci were characterized in 24 individuals from three populations located on Thousand Island Lake, Zhejiang Province, China. The number of alleles per locus varied from three to sixteen. The observed and expected heterozygosity ranged from 0.1250 to 0.8750 and 0.2333 to 0.8917, respectively. These microsatellite loci will be applied in further studies on the population genetic diversity and genetic structure of P. chinensis. This study will improve understanding of the effects of landscape fragmentation, and help conserve and manage the species.

scholarly journals Usefulness of a Locus LE21085 in the Genetic Differentiation of Tomato Varieties

2017 ◽  
Vol 9 (2) ◽  
pp. 209-213
Author(s):  
Elizabeta MISKSOKA-MILEVSKA ◽  
Zoran T. POPOVSKI ◽  
Tome NESTOROVSKI
Keyword(s):  
Genetic Diversity ◽  
Lycopersicon Esculentum ◽  
Genetic Differentiation ◽  
Allele Frequency ◽  
Base Pair ◽  
Microsatellite Loci ◽  
Individual Genome ◽  
Microsatellites Markers ◽  
Expected Heterozygosity ◽  
Polymorphic Microsatellite

The microsatellites are specific for each individual genome or species. In order to evaluate the genetic diversity and the relationships within the genus Lycopersicon, microsatellites markers were used. The main objective of this study was to determine the usefulness of the locus LE21085 in the genetic differentiation among six morphologically different tomato varieties of Lycopersicon esculentum Mill. (var. grandifolium subsp. cultum; var. cerasiforme - red and yellow, var. pruniforme, var. pyriforme subsp. subspontaneum and var. racemigerum subsp. spontaneum). For the microsatellites locus LE21085 were detected two alleles in all estimated tomato varieties, that differed by one base pair (122 and 123 bp). The biggest allele frequency was found for the allelic variant of 122 bp, and its values were: 0.8462 for L. esculentum subsp. subspontaneum var. cerasiforme (red), 0.6923 for L. esculentum subsp. subspontaneum var. cerasiforme (yellow), 0.5769 for L. esculentum subsp. cultum var. grandifolium, 0.6923 for L. esculentum subsp. subspontaneum var. pruniforme, 0.6154 for L. esculentum subsp. subspontaneum var. pyriforme and 0.8077 for L. esculentum subsp. spontaneum var. racemigerum. The average of observed heterozygosity for the locus LE21085 (Ho = 0.5641) was higher than average expected heterozygosity (He = 0.4158). The average PIC value for the locus LE21085 was 0.3294 and it was classified as a modest informative markers. From the obtained results it can be concluded that locus LE21085 could be used in genetic differentiation of tomato varieties, but in combination with other polymorphic microsatellite loci.

Isolation and characterisation of 16 novel polymorphic microsatellite loci for the Eurasian coot (Fulica atra)

2016 ◽  
Vol 64 (6) ◽  
pp. 430
Author(s):  
Tianshu Lv ◽  
Wanchao Zhu ◽  
Shuyu Xue ◽  
Huanxin Zhang ◽  
Xiaodong Gao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Genomic Library ◽  
Enriched Genomic Library ◽  
Principal Coordinates Analysis ◽  
Principal Coordinates ◽  
Fulica Atra ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite ◽  
Two Populations

To study the genetic diversity of the Eurasian coot (Fulica atra), 16 novel microsatellites were isolated and characterised using a (AC)12-enriched genomic library. Polymorphism of each locus was detected in 19 individuals from Weishan Lake and Zhaoyang Lake, both located in Shandong Province, China. These microsatellite loci revealed 3–12 alleles in two populations. One locus (Fa6) showed significant deviations from Hardy–Weinberg equilibrium in the Zhaoyang Lake population. By means of Principal Coordinates Analysis, 19 individuals were divided into two groups, which verified they were from two populations. These novel microsatellites will be useful for further studies and genetic analyses of the Eurasian coot.

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