68 Anti-Mullerian hormone levels in different bovine species and the relationship with invitro embryo production

2020 ◽  
Vol 32 (2) ◽  
pp. 159
Author(s):  
J. Berdugo ◽  
A. Tarazona-Morales ◽  
J. Echeverry-Zuluaga ◽  
W. Cardona Maya ◽  
A. López-Herrera ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Bos Indicus ◽  
Reproductive Function ◽  
Oocyte Quality ◽  
Bubalus Bubalis ◽  
P Value ◽  
Embryo Production ◽  
Individual Values ◽  
Frozen Semen ◽  
Spearman Test

The development of markers for reproductive function has been a goal for the embryo production industry and reproductive biology as a method to improve success and increase the ability to explain reproductive phenomena. Cattle and buffalo are bovines that show different reproductive parameters raised in the same conditions. The aim of this work was to compare the results of the invitro production of cows (Bos indicus) and buffalo (Bubalus bubalis) from animals kept under the same conditions of feeding and handling. This study was conducted in an Argentinian commercial herd of buffalo and cattle located in the province of Corrientes (−27.742859 latitude, −57.773611 longitude) during the breeding season of 2018 (March-May). Twenty animals of each species were used. Anti-Mullerian hormone (AMH) levels of each animal were determined using ELISA. Standardized protocols were used for oocyte aspiration, maturation, fertilisation, and culture of the embryos, and frozen semen of a single proven bull was used in each species. The numbers of follicles, oocytes, and embryos were recorded and analysed individually and grouped by species (Table 1). The normality of the data was evaluated with the D’Agostino and Shapiro-Wilk tests and the comparisons between species using the Mann-Whitney and ANOVA tests, and correlation analysis was performed using the Spearman test. Values are shown as median and range. A P-value <0.05 was considered statistically significant. The AMH levels of the cows were 688.5 pgmL−1 (45.3-2394) and the buffalo were 73.8 pgmL−1 (14.8-262.5); P<0.001. Significant differences were found in the number of recovered oocytes of 9 (0-23) for cows vs. 4.5 (1-11) for buffalo (P>0.05). There were no significant differences in the number of follicles and the quality of the oocytes. Significant differences were found in the number of oocytes cleaved of 4 (0-17) vs. 0.5 (0-4) and blastocysts per animal of 1.5 (0-15) vs. 0.1 (0-2) for cows and buffalo, respectively. The number of blastocysts in relation to the number of oocytes cleaved did not show statistical significance. In spite of the difference in the AMH levels and the embryo production parameters, which were lower in buffalo, no correlation was found between AMH and the parameters studied in both species. The results obtained here are confirmed by others in the literature. Levels of AMH as a marker for embryo production are not useful in cattle and buffalo and do not explain the differences observed, certainly AMH is a marker for quantity as the preserver of the ovarian reserve. Table 1.Comparison of individual values of AMH levels, oocyte quality, and embryo production between cattle and buffalo Parameter Median (range) P-value Cattle Buffalo AMH (pgmL−1) 688.5 (45.3-2394) 73.8 (14.8-262.5) <0.001 Follicles Follicles RO 3 (0-9) 4 (0-6) Follicles LO 5 (0-10) 4 (1-8) Total follicles 7 (2-18) 7 (2-12) Oocyte quality GI 2 (1-3) 1.5 (1-3) 0.22 GII 2 (1-9) 2 (1-6) 0.67 GIII 2.5 (0-18) 2 (1-4) 0.91 GIV 1 (1-5) 2 (1-4) 0.10 Total oocytes 9 (0-23) 4.5 (1-11) 0.02 Total viable 5.5 (0-21) 5 (0-11) 0.36 Cleavage 4 (0-17) 0.5 (0-4) 0.01 Blastocyst 1.5 (0-15) 0.1 (0-2) 0.02

scholarly journals Effects of a high-energy diet on oocyte quality and in vitro embryo production in Bos indicus and Bos taurus cows

2015 ◽  
Vol 98 (5) ◽  
pp. 3086-3099 ◽  
Author(s):  
J.N.S. Sales ◽  
L.T. Iguma ◽  
R.I.T.P. Batista ◽  
C.C.R. Quintão ◽  
M.A.S. Gama ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Bos Indicus ◽  
Oocyte Quality ◽  
High Energy ◽  
Embryo Production ◽  
In Vitro Embryo Production

293 FACTORS ASSOCIATED WITH PREGNANCY OUTCOMES OF IN VITRO-PRODUCED EMBRYOS IN ZEBU CATTLE

2010 ◽  
Vol 22 (1) ◽  
pp. 303
Author(s):  
L. A. Oyuela ◽  
J. Olaya ◽  
J. Zambrano-Varón ◽  
C. Jiménez
Keyword(s):  
Pregnancy Rate ◽  
Logistic Model ◽  
Pregnancy Loss ◽  
Statistical Significance ◽  
Bos Indicus ◽  
Estradiol Valerate ◽  
P Value ◽  
Zebu Cattle ◽  
Factors Associated

Few studies have addressed the factors that can affect pregnancy rates of in vitro-produced embryos (IVPE) and fewer still when referring to zebu IVPE. This study aimed to estimate some of these factors and the proportion of pregnancy loss. A total of 1227 pure-breed IVPE (Brahman, Red Brahman, and Gyr) were transferred (ET) to an equal number of crossbred Bos indicus × Bos taurus heifers. Recipients averaged 420 kg, were grass fed, and kept in three different ET Centrals located in northern, central, and eastern regions of Colombia. IVPE (Embriogen, Bogotá, Colombia) were nonsurgically transferred into synchronized recipients by using the following protocol: Day 0 s.c. implant of norgestomet 3 mg, plus an i.m. injection of estradiol valerate 5 mg and norgestomet 3 mg; Day 7 D-cloprostenol 0.15 mg i.m. Day 9 implant removal plus 200IU of eCGi.m. Pregnancy was recorded on Days 30 and 60 by ultrasound with an 8-m Hz linear rectal probe. Data were collected for 1 year beginning in September 2007. Binary logistic regression with forward conditional elimination was performed to evaluate factors associated with pregnancy. The strength of the association between the studied variables and pregnancy rate was estimated by the odds ratio (OR) (95% CI); a P-value < 0.05 indicated statistical significance. Interaction terms were considered (SPSS Inc., Chicago, IL, USA). Variables included in the logistic model were CL diameter at the time of ET, number of previous transfers to a recipient before achieving pregnancy, difficulty during the ET, embryo quality and stage, technician and level of training, number of transferred embryos by technician per day, uterine horn where the embryo was delivered, time between laboratory release and ET, ET Central, period of the year when the ET was performed, sire and breed of the embryo. Total pregnancy rate was 38% on Day 60 (462/1227), and the proportion of pregnancy loss was 8% between Days 30 and 90. Factors associated with pregnancy were as follows: 1) excellent embryos were 2.3 times more likely to achieve pregnancy (39%) than good embryos (21%), OR = 2.3, 95%CI = 1.4-3.8, P = 0.001; 2) easy transferred embryos were 5.1 times more likely to result in pregnancy (49%) compared with difficult transferred embryos (11%), OR = 5.1, 95% CI = 2.5-10.3, P < 0.05; and 3) transferred embryos <5 h after being released from the laboratory resulted in higher pregnancy rate (42%) than those transferred >5 h (33%). No interactions were found in the final logistic model. In conclusion, pregnancy outcome of IVPE Bos indicus was strongly associated with quality of the embryo, level of difficulty at the moment of the ET, and the time between the production of the embryo and the ET. In addition, the observed low pregnancy loss in this study suggests careful selection and management of the recipients, adequate handling of the IVPE, and proper training of the ET technicians. Embriogen S.A. Bogotá, Colombia. Cenatte, Pedroleopoldo, MG, Brazil.

193 Effect of clinical metritis on oocyte recovery, oocyte quality, and early invitro developmental competence of embryos in Bos indicus dairy cattle

2020 ◽  
Vol 32 (2) ◽  
pp. 225
Author(s):  
M. Saleem ◽  
Z. Sarwar ◽  
M. Saad ◽  
I. Zahoor ◽  
N. Ahmad ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Recovery Rate ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Oocyte Quality ◽  
Developmental Competence ◽  
Cleavage Rate ◽  
Healthy Group ◽  
Oocyte Recovery ◽  
Frozen Semen

Unhygienic practices at the time of parturition or AI lead to uterine infections. The uterine infections ultimately result in genetic drain by culling the elite animals. The invivo developmental competence of embryos is compromised in clinically metritic animals. The genetic potential of problematic females could be harvested by invitro embryo production (IVEP). Therefore, the objective of the present study was to evaluate the effect of clinical metritis on oocyte recovery, oocyte quality, and early invitro developmental competence of embryos in Bos indicus dairy cattle. This experiment was carried out from December 2017 to April 2018. Ovaries were collected from a local abattoir (Bos indicus; 5- to 8-year-old dairy cattle, body condition score 2.75±0.25, mixed parity). These ovaries (n=982) were divided into two groups: (1) clinically metritic (n=184), and (2) healthy (n=798), based upon the presence or absence of pus in the uterine lumen. Oocytes were aspirated from follicles using an 18G needle attached to a 10-mL syringe. Cumulus-oocyte complexes (COCs) were categorized into A, B, C, and D grades based on the number of layers of cumulus cells and integrity of ooplasm. The oocytes of grades A and B were subsequently transferred in groups (10/group) in four-well plates containing 100-μL droplets. The droplets with oocytes were covered with prewarmed mineral oil and incubated for 24h at 38.5°C, 5% CO2, and 95% relative humidity. The oocytes were evaluated for IVM on the basis of cumulus expansion. Frozen semen was thawed and prepared using the sperm swim-up procedure for each group. Spermatozoa and oocytes were incubated together for a period of 18h. The presumptive zygotes were invitro cultured for 4 days in a CO2 incubator under similar culture conditions. The cleavage rate, 4-cell, and 8-cell stages were recorded on Days 2, 3, and 4 after the day of insemination, respectively. Data on oocyte recovery, oocyte quality, IVM, cleavage rate, and 4-cell and 8-cell stages were analysed by Chi-squared test using SPSS software (version 20; IBM Corp.) for Windows. Results demonstrated that recovery rate was lower (63.8% vs. 71.7%; P&lt;0.05) in clinically metritic compared with healthy cattle. Similarly, oocytes of grade A and B quality were lower (41.0% vs. 51.1%; P&lt;0.05), whereas those of C and D quality were higher (59.0% vs. 48.9%; P&lt;0.05) in clinically metritic compared with the healthy group. Moreover, 4-cell (38.2% vs. 54.8%) and 8-cell stage embryos (11.3% vs. 29.1%), were lower (P&lt;0.05) in the clinically metritic compared with the healthy group, respectively. However, maturation rate and cleavage rate did not differ (P&gt;0.05) between groups. In conclusion, metritis in slaughterhouse ovaries negatively affects oocyte recovery rate, oocyte quality, and early invitro developmental competence of embryos in Bos indicus dairy cattle.

130 In vitro embryo production using frozen semen from cloned and non-cloned Bos indicus bulls

2019 ◽  
Vol 31 (1) ◽  
pp. 190
Author(s):  
S. Romo ◽  
O. Sebastián ◽  
F. Guerrero ◽  
R. Romero ◽  
F. Muñoz ◽  
...  
Keyword(s):  
Bos Indicus ◽  
The State ◽  
Assisted Reproductive Techniques ◽  
Embryo Production ◽  
Humid Atmosphere ◽  
Frozen Semen ◽  
Significant Difference ◽  
Reproductive Techniques ◽  
In Vitro Embryo Production

Reproductive biotechnology has continued to evolve rapidly, allowing the development of techniques to increase reproductive efficiency and contribute to the genetic improvement of cattle. Some of these techniques include the in vitro maturation and IVF of oocytes, sperm sexing and cloning. These modern assisted reproductive techniques can help produce offspring of desired genetic characteristics and of a pre-determined sex. However, studies of the bull’s contribution to in vitro reproductive performance are scarce in the Brahman breed. The aim of this study was to compare oocyte maturation and embryo production in vitro using frozen semen from 5 Brahman bulls (Bos indicus), cloned (n=1) and non-cloned (n=4), with characteristics and genetics of high commercial value. The age of the bulls at the time of semen collection and cryopreservation ranged from 2 to 7 years. The oocytes were obtained on 2 different dates (45 days between collections) using pooled oocytes collected by ovum pickup at random stages of the oestrous cycle, from a total of 15 Brahman donor cows. Oocytes were transported to a laboratory in the State of Chiapas, Mexico (Genemex Internacional). The oocytes were cultured in maturation medium for 24h. For IVF, conventional semen was used from one bull (B1) and his clone (B12), the grandson of B1 (B2), and 2 non-related bulls (B3 and B4). The gametes were co-incubated for 22h and afterward placed in medium for embryo development and cultured for 7 days in a humid atmosphere with 5% CO2 in air. Of the matured oocytes, 36/43 (84%), 16/32 (50%), 101/143 (70%), 46/67 (68%) and 53/65 (81%) were fertilized using semen from B1, B12, B2, B3 and B4, respectively. Of the fertilized oocytes, 15/30 (50%), 8/16 (50%), 45/101 (44%), 21/46 (45%) and 18/53 (34%) resulted in transferrable embryos, corresponding to semen from the same bulls, respectively. This would appear to be the first scientific report in Mexico about the use of semen from a cloned bull for in vitro embryo production. In IVF, similar results were observed between B1 and a non-related bull (B4). Similar results in transferrable embryos were observed between B1 and B12 but also similar to a related bull (B3) and a non-related bull (B4). A Fisher’s exact test of the IVF results comparing B1 and B12 found a significantly (P&lt;0.05) higher number of fertilized oocytes for B1. However, a significant difference was not found (P&gt;0.05) concerning the number of transferrable embryos produced by these two bulls. In conclusion, the Brahman bulls in this study differ in their contribution to IVF and embryo production. Further studies are required to determine the factors responsible for such effects, e.g. age differences or clone versus non-clone mosaicism. Results from this research contribute to the study and development of assisted reproductive techniques for increasing in vitro production efficiency in Zebu cattle. We thank the Rosales family, from El Herradero Ranch, in the State of Campeche, Mexico, for allowing the use of their cattle for this project.

196 Fertilization Rate in Superovulated Criolla Goats Following Artificial Insemination or Natural Mating

2018 ◽  
Vol 30 (1) ◽  
pp. 238
Author(s):  
M. I. Cueto ◽  
J. Fernandez ◽  
M. M. Bruno-Galarraga ◽  
F. Pereyra-Bonnet ◽  
A. Gibbons
Keyword(s):  
Statistical Significance ◽  
Genetic Material ◽  
Fertilization Rate ◽  
Corpora Lutea ◽  
Embryo Production ◽  
Transfer Programs ◽  
Embryo Recovery ◽  
Frozen Semen ◽  
Natural Mating ◽  
Material Studies

The Criolla breed is a local genetic resource, distributed in Patagonia, Argentina, whose primary production is meat. In the Criolla goat, efforts were made to locate productively superior males and to conserve their genetic material. Studies were carried on the feasibility of obtaining superior offspring through the implementation of embryo transfer programs. We assessed the fertilization rate and embryo production following AI with frozen semen or natural mating in Criolla goats subjected to a superovulation program. During the breeding season (May, 41° S), 26 Criolla goats were treated for oestrus with sponges (60 mg of medroxyprogesterone acetate, Progespon®, Syntex, Buenos Aires, Argentina) placed for 13 days. Goat donors were superovulated with a total of 80 mg of porcine (p)FSH (Folltropin V®, Bioniche, Ontario, Canada) every 12 h in 6 decreasing doses (18, 18, 14, 14, 8, and 8 mg) during the last 3 days of progestagen treatment. A dose of 125 μg of cloprostenol (Estrumate®, Schering-Plough, Quebec, Canada) was given in conjunction with the first dose of pFSH. Oestrus detection was performed every 12 h, starting at 24 h after sponge removal. Females were considered to be in oestrus if they passively accepted buck mounting. Goats were randomly assigned to the following treatments: (1) natural mating (NM, n = 12): donors detected in oestrus were individually mated with one proven fertile buck at oestrus and 12 h post-oestrus; does were remove from the male in between (Conventionally accepted treatment); (2) AI (n = 14): donors detected in oestrus were inseminated 12 ± 2 h after the onset of oestrus by laparoscopy with frozen-thawed semen (200 × 106 spermatozoa) from the same fertile buck. Embryo recovery was done by surgical prepubic laparotomy at Day 8 after sponge removal. Superovulation response was estimated by counting the number of corpora lutea (CL). Analysis of variance was used to compare fertilization rate (total number of embryos recovered for each animal, expressed as a proportion of the total number of embryos/oocytes recovered) and embryo production between treatments. Results were expressed as mean ± SEM. Statistical significance was accepted at P < 0.05. A total of 92.3% goats were recorded in oestrus (24/26) between 24 and 48 h after sponge removal (10 and 14 goats for NM and AI, respectively). An average of 16.6 ± 2.0 CL (range: 2–32) was observed in response to superovulation treatment. The recovery rate of embryos/oocytes was 60.0 ± 6.9%. No statistical difference was observed in the fertilization rate (52.1 ± 12.1 and 68.6 ± 12.1% for AI and NM, respectively) or the number of total (6.8 ± 2.0 and 4.8 ± 2.4 for AI and NM, respectively) and transferable embryos (5.6 ± 1.4 and 4.0 ± 1.7 for AI and NM, respectively) between treatments (P > 0.05). In conclusion, fertilization rates did not differ following laparoscopic insemination with frozen semen compared to natural mating in superovulated Criolla goats.

scholarly journals 261DIFFERENT TRANSVAGINAL OVUM PICK-UP STRATEGIES TO OPTIMIZE THE OOCYTE RETRIEVAL AND EMBRYO PRODUCTION OVER A FIXED PERIOD OF TIME

2004 ◽  
Vol 16 (2) ◽  
pp. 250
Author(s):  
S. Chaubal ◽  
J. Molina ◽  
C. Olrichs ◽  
L. Ferré ◽  
D. Faber ◽  
...  
Keyword(s):  
Oocyte Retrieval ◽  
Oocyte Quality ◽  
Single Shot ◽  
Embryo Production ◽  
Chi Square ◽  
Frozen Semen ◽  
Chi Square Test ◽  
Positive Effect ◽  
Fixed Period ◽  
Ultrasound Scanner

The objective of this study was to compare the efficacy of different oocyte retrieval schemes over a period of 10 weeks. Fifteen multiparous Angus cows were randomly assigned (n=3/group) to the following groups: 1) OPU once/week (7-day interval), 2) OPU twice/week (3- and 4-day interval, alternately), 3) Dominant follicle removal (DFR)+OPU once/week. DFR followed by OPU 72h later, 4) DFR+FSH+OPU once/week. DFR followed 36h later by FSH (Folltropin, Bioniche, Belleville, Ontario, Canada) (120mg s.c. and 80mg i.m. administered simultaneously) followed by OPU 48h later, 5) FSH+OPU twice/week. FSH followed by OPU1 30h later and OPU2 96h after OPU1. The interval between OPU2 and next FSH was approximately 42h. The follicles were aspirated using an Aloka ultrasound scanner (Wallingford, CT, USA) and a 5-MHz probe. The COCs were selected based on morphology and matured in TCM-199, supplemented with 10% FCS, 0.01 unitsmL−1 bFSH, 0.01 unitsmL−1 bLH and antibiotics. Fertilization (Day 0) was carried out with TALP-FERT medium containing capacitation factors. Frozen semen from the same bull was used (1×106mL−1) throughout. After 18h the presumptive zygotes were cultured in SOF with 5% FCS (Holm P et al., 1999 Theriogenology 52, 683–700). The embryos were evaluated based on IETS guidelines (Grades 1 and 2 selected). The data were analyzed by chi-square test and ANOVA. In all parameters, the DFR followed by FSH and subsequent OPU once/week protocol gave the best results on a per-cow-per-week as well as total (3 cows over 10 weeks) basis. Though OPU was done only once/week, this group produced more total oocytes (303) than groups where OPU was done twice/week, either with FSH (286) or without FSH (229) and also produced more total embryos on Day 8 (71 blastocysts, 23.4% of oocytes cultured) than the latter two groups (64, 22.4% and 49, 21.4%, respectively). Among the nonstimulated groups, the OPU twice/week group had more total oocytes (236) than groups with OPU once/week, either without DFR (137) or with preceding DFR (160). However, a preceding DFR seemed to have a positive effect on oocyte quality as this group had a better embryo development rate (26.9%), producing more total embryos (42). In comparison, OPU twice/week produced total 49 embryos (21.4%) and OPU once/week produced 26 (19.4%). In conclusion, DFR coupled with single-shot FSH administration can be used effectively over a period of at least 10 consecutive weeks and can increase (P&lt;0.05) the oocyte yield by two-fold and embryo production following IVF by two and half-fold, as compared to routine OPU-IVF done once a week. Table 1 Per cow per week performance

245 EFFECTS OF Gyr (BOS TAURUS INDICUS) DONOR MITOCHONDRIAL DNA AND OVUM PICKUP ORDER ON IN VITRO EMBRYO PRODUCTION

2015 ◽  
Vol 27 (1) ◽  
pp. 212
Author(s):  
T. L. C. Pinto ◽  
B. C. Lopes ◽  
M. B. D. Ferreira ◽  
T. M. Goncalves ◽  
J. C. Souza ◽  
...  
Keyword(s):  
Mixed Model ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Covariance Structure ◽  
Oocyte Quality ◽  
Embryo Production ◽  
Bos Taurus Indicus ◽  
Hindiii Restriction Site ◽  
In Vitro Embryo Production

Ovum pick (OPU) up is a technique upon which in vitro embryo production (IVP) depends. It permits donor cow maternal ancestry to be assessed by mtDNA analysis. Repetitive ablation of follicles is thought to interfere with the donor cow endocrine profile and influence embryo yield. The objective was to evaluate the effects of mtDNA and OPU order on IVP fertility traits. Gyr donors (85) were submitted to 363 OPU sessions (5 OPU sessions/donor). Donor mtDNA was extracted from leukocytes and sorted by the presence of the HindIII restriction site within the amplified region, indicative of Bos taurus taurus mtDNA (Paneto et al. 2008 Genet. Mol. Res. 7, 592). All animals in the donor pedigree were classified, and the population was divided into two groups according to their maternal genetic grouping: Bos taurus indicus and Bos taurus taurus. For statistical analyses, data from 5 OPU sessions per each donor were submitted to the mixed model procedure of SAS® (SAS Institute Inc., Cary, NC, USA), using the lowest Akaike value to determine the best covariance structure between repeated OPU session results. The model included the effect of OPU session order (1–5) and mtDNA-based maternal grouping (Bos taurus v. Bos indicus) as independent variables. Total and viable oocytes, as well as blastocyst yield per OPU session, were the dependent variables studied. The means of total and viable oocytes and blastocysts produced per donor per OPU were compared by the Tukey test, at 5% significance. The combined OPU sessions resulted in 6084 oocytes, 2537 embryos, which produced 1105 pregnancies. Mean numbers (n = 42 donors/OPU session) of total and viable oocytes between OPU sessions 1 (31.9 ± 4.6 and 19.2 ± 2.9), 2 (35.3 ± 3.5 and 21.2 ± 2.2), 3 (28.9 ± 3.7 and 19.3 ± 2.4), 4 (25.0 ± 5.2 and 18.9 ± 33), and 5 (20.5 ± 6.3 and 13.8 ± 4.0) did not differ. Mean blastocyst production after IVP between OPU sessions 1 (6.5 ± 1.4), 2 (5.15 ± 1.04), 3 (5.5 ± 1.1), 4 (5.8 ± 1.6), and 5 (5.2 ± 1.9) was similar. Mean viable oocyte number was greater (P < 0.0001) for B. t. taurus (21.7 ± 1.4, n = 192 OPU sessions) compared with B. t. indicus (16.1 ± 0.9, n = 138 OPU sessions) maternal genetic groupings. However, in vitro blastocyst yields were similar (P = 0.23) between maternal genetic groupings (7.3 ± 1.6 and 6.2 ± 1.4 for B. taurus and B. indicus, respectively). In conclusion, repeated OPU sessions did not reduce oocyte and embryo yields as expected. Maternal B. taurus genetic origin was associated with higher oocyte quality, although it was not translated into higher embryo yields after in vitro culture. Results warrant further research, which may result in additional selection criteria for OPU Gyr donors considering their maternal genetic background.

224 Superstimulation effect on invitro embryo production and relationship with anti-Müllerian hormone in buffaloes (Bubalus bubalis) aspirated 7 or 14 days after follicular ablation

2020 ◽  
Vol 32 (2) ◽  
pp. 240
Author(s):  
J. Konrad ◽  
J. A. Berdugo Gutierrez ◽  
R. Yuponi ◽  
N. Vallejos ◽  
W. Cardona-Maya ◽  
...  
Keyword(s):  
Strong Correlation ◽  
Body Condition Score ◽  
Selection Criterion ◽  
Residual Effect ◽  
Oocyte Quality ◽  
Bubalus Bubalis ◽  
Residual Effects ◽  
Embryo Production ◽  
Standard Curve ◽  
Beneficial Effects

The success of buffalo invitro embryo production (IVEP) depends, in part, on the number and competence of oocytes. Superstimulation with FSH has been linked to oocyte quality and embryo yield, and anti-Müllerian hormone (AMH) has been proposed as an indicator of ovarian reserve. The objective was to evaluate FSH stimulation in one ovum pick-up (OPU) cycle conducted 7 or 14 days after follicular ablation; we additionally analysed residual FSH treatment effect carried over to a subsequent aspiration cycle. Finally, we evaluated the relationship between plasma AMH and FSH response. Nine multiparous, cycling Murrah donors (603±7.0kg, body condition score=3.5) were subjected to complete follicular ablation followed by OPU 1 week later (T0 Control), and plasma AMH was determined at this point. They were then randomly allocated to FSH stimulation (3 days b.i.d. in descending doses, total 300mg of pFSH, Vetpharm, with the last injection 48h before OPU) with aspiration conducted 7 (T7 FSH, n=5) or 14 days (T14 FSH, n=4) post-follicular ablation. The residual effect (T Residual, n=5) was evaluated in one subsequent, nonstimulated OPU cycle conducted 1 week later (9 days after last FSH administration). For plasma AMH, concentration was measured using ELISA kit (cat. no. AL114, AnshLabs; sensitivity 1.0 pgmL−1, interassay variation &lt;5%, additional validation conducted with serial dilutions of buffalo plasma, r=0.98 against standard curve). Continuous data were analysed with ANOVA and Tukey's post hoc comparisons; categorical data were analysed using Chi-squared test. Pearson coefficient was used to evaluate correlation between AMH, follicular reserve, and invitro performance. Superovulatory treatment resulted in a higher number of follicles and good quality oocytes (grades 1 and 2) per donor, irrespective of OPU interval (T7 FSH: 3.0±2.55 and T14 FSH: 1.5±0.5 vs. T0 Control: 0.45±0.38 and T Residual: 0.4±0.4; P&lt;0.05). There were more cleaved embryos (T7 FSH: 26%, T14 FSH; 30% vs. T0 Control: 7% and T Residual: 20%) and blastocysts (T7 FSH: 21%, T14 FSH; 30% vs. T0 Control: 5% and T Residual: 0; P&lt;0.05) from FSH-treated donors. There was a strong correlation (r=0.9439) between follicle count and plasma AMH, and weaker relationships for oocytes recovered (r=0.3975), cleaved embryos (r=0.2317), or blastocysts (r=0.0106). Results indicate that FSH treatment with a 48-h coasting period results in significant improvements in follicular population, oocyte quality, and embryonic development; these effects are observed independently of the interval between initial follicular ablation and OPU. No residual effects of FSH treatment were observed, indicating that the beneficial effects of superstimulation are not carried over to the following OPU cycle. In conclusion, a strong correlation coefficient was established between initial plasma AMH level and follicular response to FSH treatment, indicating that it may be used as a selection criterion for animals likely to exhibit a good response to superstimulatory treatment.

SERUM PROLACTINE LEVEL IN PATIENTS OF PSORIASIS VULGARIS AND ITS CORRELATION WITH DISEASE SEVERITY: A CASE-CONTROL STUDY

2019 ◽  
Vol 3 (6) ◽  
Author(s):  
Pawan Kumar Saini ◽  
Devendra Yadav ◽  
Rozy Badyal ◽  
Suresh Jain ◽  
Arti Singh ◽  
...  
Keyword(s):  
Disease Severity ◽  
Prolactin Level ◽  
Case Control Study ◽  
Statistical Significance ◽  
Case Control ◽  
Serum Prolactin ◽  
P Value ◽  
Serum Prolactin Level ◽  
R Value ◽  
Control Study

Background: Psoriasis is an autoimmune chronic inflammatory disorder affecting the skin mediated by T-lymphocytes resulting in production of cytokines which cause hyperproliferation of keratinocytes.  Several factors and hormones like Prolactin have an action similar to these cytokines in promoting the multiplication of keratinocytes and other cells like lymphocytes and epithelial cells may have a role on the etiopathogenesis of psoriasis. Aim:-The aim of study is to compare the serum Prolactin levels in patients of psoriasis with a control group. Setting and study design: This is a case-control study conducted in the department of Dermatology, Venereology and Leprosy GMC, Kota over a period of 1year from July 2017 to June 2018 Material and method: The study included 100 cases of psoriasis (60 males and 40 females) and 100 controls similar for age and sex. Serum Prolactin levels were measured by ECLIA and results were obtained. Statistical analysis: Mean and standard deviation were calculated for each variable. Statistical significance of the results was analyzed using correlation analysis (Pearson correlation coefficient) and independent samples t-test. Statistical significance was assumed at p value<0.05. Result: Serum Prolactin level was significantly higher in cases of psoriasis compared to controls (p-value <0.001). PASI score and serum Prolactin levels were found to have a positive correlation (r value = 0.337; p-value: 0.001). No significant  correlation was found between serum levels of Prolactin and duration of disease r value= -0.034, P value =0.733). Serum Prolactin level was higher in male patients compared to females patients. Conclusion:- High serum Prolactin may be a biological marker of disease severity in psoriasis and may have a role in the pathogenesis of psoriasis. Further studies with large sample size are required to confirm this hypothesis.

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