Determination of porcine endometrial phospholipase A2 activity and detection of immunoreactive cyclooxygenase during the oestrous cycle and early pregnancy

1993 ◽  
Vol 5 (5) ◽  
pp. 531 ◽  
Author(s):  
DH Dubois ◽  
LC Smith ◽  
FW Bazer
Keyword(s):  
Phospholipase A2 ◽  
Early Pregnancy ◽  
Oestrous Cycle ◽  
Surface Epithelium ◽  
Intense Staining ◽  
Porcine Conceptus ◽  
Uterine Glands ◽  
Increased Activity ◽  
Phospholipase A2 Activity

Experiments examined the characteristics and activity of phospholipase A2 (PLA2) and examined the presence of immunoreactive cyclooxygenase in endometrium of pigs during the oestrous cycle and early pregnancy. Endometrial PLA2 was calcium-independent and activity of the enzyme was greatest at a pH of 8.0. Activity of PLA2 on Days 10, 12, 14 and 16 of the oestrous cycle did not differ (P > 0.1) from activity on those days during pregnancy. During oestrus and early metoestrus (Days 0-3), cyclooxygenase was present in both glandular and surface epithelium. After Day 10 of the oestrous cycle or pregnancy, staining for cyclooxygenase was less intense in the lower and middle uterine glands. However, the upper glandular epithelium near the surface epithelium stained intensely. By Day 15 of the oestrous cycle or pregnancy, intense staining for cyclooxygenase appeared restricted to the upper uterine glands. These results indicate changes in localization of immunoreactive cyclooxygenase throughout the oestrous cycle and suggest that these are not related to altered secretion of prostaglandins (PGs) during early pregnancy. The stimulatory effects of porcine conceptus products on secretion of PGs during early pregnancy are apparently not associated with increased activity of endometrial PLA2.

Immunocytochemical localization and changes in endometrial progestin receptor protein during the porcine oestrous cycle and early pregnancy

1994 ◽  
Vol 6 (6) ◽  
pp. 749 ◽  
Author(s):  
RD Geisert ◽  
TN Pratt ◽  
FW Bazer ◽  
JS Mayes ◽  
GH Watson
Keyword(s):  
Progesterone Receptor ◽  
Early Pregnancy ◽  
Cellular Localization ◽  
Oestrous Cycle ◽  
Receptor Protein ◽  
Surface Epithelium ◽  
Immunocytochemical Localization ◽  
Intense Staining ◽  
Pr Protein ◽  
Progestin Receptor

Changes of progesterone receptor (PR) protein and cellular localization in the endometrium were evaluated during the oestrous cycle and early pregnancy of the gilt. During the oestrous cycle, the concentration of total PR protein within the endometrium was highest on Days 0-5 and decreased on Day 10. The endometrial concentration of PR reached a nadir on Day 12 and this level was maintained throughout the remainder of the oestrous cycle (Day 18). In pregnant gilts, the concentration of endometrial PR protein from Day 10 to Day 18 was similar to that in cyclic gilts. Western blot analysis with antiserum specific for the A and B isoforms of PR indicated that porcine endometrium expresses both isoforms of PR. Immunostaining was detectable for both the A and B isoforms of PR from Day 0 to Day 12 of the oestrous cycle. However, no staining was observed on Day 15 and Day 18 of the oestrous cycle or pregnancy Immunocytochemical localization of PR in the endometrium of cyclic gilts and pregnant gilts indicated that there was intense staining for PR in surface epithelium and glandular epithelium during oestrus (Day 0) and on Day 5. However, the staining was less intense on Day 7 and Day 10 of the oestrous cycle and no epithelial staining was observed after Day 12. PRs were present in the stroma and myometrium throughout the oestrous cycle and early pregnancy. The presence of conceptuses during pregnancy did not affect the loss of PR from the uterine epithelium after Day 10 of gestation. Down-regulation of epithelial PR might be one factor involved in the timing of luteolysis during the oestrous cycle as well as conceptus growth and placentation during early pregnancy.

P3-173: Determination of a specific lipoprotein-associated phospholipase A2 activity in human cerebrospinal fluid

2010 ◽  
Vol 6 ◽  
pp. S500-S500
Author(s):  
Liang Ye ◽  
Tina Li ◽  
Daniel Lee ◽  
Curtis Huang ◽  
Chenbing Guan ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Phospholipase A2 ◽  
Human Cerebrospinal Fluid ◽  
Phospholipase A2 Activity

Impedimetric Biosensor for the Determination of Phospholipase A2 Activity in Snake Venom

2017 ◽  
Vol 51 (3) ◽  
pp. 401-410 ◽  
Author(s):  
Nedjla Zehani ◽  
Wilairat Cheewasedtham ◽  
Rochdi Kherrat ◽  
Nicole Jaffrezic-Renault
Keyword(s):  
Phospholipase A2 ◽  
Snake Venom ◽  
Impedimetric Biosensor ◽  
Phospholipase A2 Activity

A continuous fluorescence assay for the determination of calcium-dependent secretory phospholipase A2 activity in serum

2007 ◽  
Vol 379 (1-2) ◽  
pp. 119-126 ◽  
Author(s):  
Francis H.C. Tsao ◽  
Dhanansayan Shanmuganayagam ◽  
Derek K. Zachman ◽  
Mehdi Khosravi ◽  
John D. Folts ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Fluorescence Assay ◽  
Secretory Phospholipase A2 ◽  
Secretory Phospholipase ◽  
Calcium Dependent ◽  
Phospholipase A2 Activity

scholarly journals Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy

Reproduction ◽  
2005 ◽  
Vol 129 (3) ◽  
pp. 349-359 ◽  
Author(s):  
S Sukjumlong ◽  
A-M Dalin ◽  
L Sahlin ◽  
E Persson
Keyword(s):  
Monoclonal Antibody ◽  
Progesterone Receptor ◽  
Early Pregnancy ◽  
Artificial Insemination ◽  
Oestrous Cycle ◽  
Surface Epithelium ◽  
Physiological Changes ◽  
Receptor Proteins ◽  
High Level ◽  
Immunohistochemical Studies

Physiological changes in the sow uterus involve the regulation by progesterone and its receptor proteins (PR). Therefore, the aim of the present study was to investigate the localization of PR during different stages of the oestrous cycle and in inseminated sows during early pregnancy by use of immunohistochemistry. Uterine samples were collected from cyclic and inseminated sows at different stages of the oestrous cycle and early pregnancy. The samples were fixed in 10% formaldehyde and embedded in paraffin. Immunohistochemistry was done by use of a mouse monoclonal antibody to PR. The highest PR immunostaining in the surface epithelium was observed at oestrus/5–6 h after artificial insemination (AI) and early dioestrus/70 h after AI. In the glandular epithelium, the highest level of PR was found at oestrus with the lowest at late dioestrus/d 19. Higher levels of PR were observed in inseminated groups compared with cyclic sows. In the myometrium, a high level of PR was found at oestrus, while stromal PR cells were constantly present throughout the oestrous cycle and at different stages of early pregnancy. In conclusion, this study shows that the immunopresence of PR in the sow uterus differed between uterine compartments at the same reproductive stage. Differences were also found for some uterine compartments between cyclic and inseminated/early pregnant sows. The relatively consistent immunostaining of PR in the stroma strengthens a stromal role in the regulation of physiological activities in the sow uterus during the oestrous cycle as well as early pregnancy.

A rapid fluorometric assay for the determination of serum phospholipase A2 activity

2009 ◽  
Vol 91 (1) ◽  
pp. 82-84 ◽  
Author(s):  
M. Merchant ◽  
J. Small ◽  
R. Falconi ◽  
B.J. Crookshank
Keyword(s):  
Phospholipase A2 ◽  
Fluorometric Assay ◽  
Phospholipase A2 Activity
Sign in / Sign up

Export Citation Format

Share Document