A rapid fluorometric assay for the determination of serum phospholipase A2 activity

2009 ◽  
Vol 91 (1) ◽  
pp. 82-84 ◽  
Author(s):  
M. Merchant ◽  
J. Small ◽  
R. Falconi ◽  
B.J. Crookshank
Keyword(s):  
Phospholipase A2 ◽  
Fluorometric Assay ◽  
Phospholipase A2 Activity

P3-173: Determination of a specific lipoprotein-associated phospholipase A2 activity in human cerebrospinal fluid

2010 ◽  
Vol 6 ◽  
pp. S500-S500
Author(s):  
Liang Ye ◽  
Tina Li ◽  
Daniel Lee ◽  
Curtis Huang ◽  
Chenbing Guan ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Phospholipase A2 ◽  
Human Cerebrospinal Fluid ◽  
Phospholipase A2 Activity

Impedimetric Biosensor for the Determination of Phospholipase A2 Activity in Snake Venom

2017 ◽  
Vol 51 (3) ◽  
pp. 401-410 ◽  
Author(s):  
Nedjla Zehani ◽  
Wilairat Cheewasedtham ◽  
Rochdi Kherrat ◽  
Nicole Jaffrezic-Renault
Keyword(s):  
Phospholipase A2 ◽  
Snake Venom ◽  
Impedimetric Biosensor ◽  
Phospholipase A2 Activity

A continuous fluorometric assay for phospholipase A2 activity in brain cytosol

2000 ◽  
Vol 100 (1-2) ◽  
pp. 127-133 ◽  
Author(s):  
Danny E. Yarger ◽  
Casey B. Patrick ◽  
Stanley I. Rapoport ◽  
Eric J. Murphy
Keyword(s):  
Phospholipase A2 ◽  
Fluorometric Assay ◽  
Phospholipase A2 Activity ◽  
Brain Cytosol

A continuous fluorescence assay for the determination of calcium-dependent secretory phospholipase A2 activity in serum

2007 ◽  
Vol 379 (1-2) ◽  
pp. 119-126 ◽  
Author(s):  
Francis H.C. Tsao ◽  
Dhanansayan Shanmuganayagam ◽  
Derek K. Zachman ◽  
Mehdi Khosravi ◽  
John D. Folts ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Fluorescence Assay ◽  
Secretory Phospholipase A2 ◽  
Secretory Phospholipase ◽  
Calcium Dependent ◽  
Phospholipase A2 Activity

Determination of porcine endometrial phospholipase A2 activity and detection of immunoreactive cyclooxygenase during the oestrous cycle and early pregnancy

1993 ◽  
Vol 5 (5) ◽  
pp. 531 ◽  
Author(s):  
DH Dubois ◽  
LC Smith ◽  
FW Bazer
Keyword(s):  
Phospholipase A2 ◽  
Early Pregnancy ◽  
Oestrous Cycle ◽  
Surface Epithelium ◽  
Intense Staining ◽  
Porcine Conceptus ◽  
Uterine Glands ◽  
Increased Activity ◽  
Phospholipase A2 Activity

Experiments examined the characteristics and activity of phospholipase A2 (PLA2) and examined the presence of immunoreactive cyclooxygenase in endometrium of pigs during the oestrous cycle and early pregnancy. Endometrial PLA2 was calcium-independent and activity of the enzyme was greatest at a pH of 8.0. Activity of PLA2 on Days 10, 12, 14 and 16 of the oestrous cycle did not differ (P > 0.1) from activity on those days during pregnancy. During oestrus and early metoestrus (Days 0-3), cyclooxygenase was present in both glandular and surface epithelium. After Day 10 of the oestrous cycle or pregnancy, staining for cyclooxygenase was less intense in the lower and middle uterine glands. However, the upper glandular epithelium near the surface epithelium stained intensely. By Day 15 of the oestrous cycle or pregnancy, intense staining for cyclooxygenase appeared restricted to the upper uterine glands. These results indicate changes in localization of immunoreactive cyclooxygenase throughout the oestrous cycle and suggest that these are not related to altered secretion of prostaglandins (PGs) during early pregnancy. The stimulatory effects of porcine conceptus products on secretion of PGs during early pregnancy are apparently not associated with increased activity of endometrial PLA2.

Determination of phospholipase A2 activity by a colorimetric assay using a pH indicator

Toxicon ◽  
1987 ◽  
Vol 25 (11) ◽  
pp. 1181-1188 ◽  
Author(s):  
Albetiza Lōbo de Araújo ◽  
François Radvanyi
Keyword(s):  
Phospholipase A2 ◽  
Colorimetric Assay ◽  
Ph Indicator ◽  
Phospholipase A2 Activity
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