scholarly journals Effect of Sodium Nutrition on Chlorophyll a/b Ratios in C4 Plants

1984 ◽  
Vol 11 (4) ◽  
pp. 325 ◽  
Author(s):  
M Johnston ◽  
CPL Grof ◽  
PF Brownell
Keyword(s):  
Chlorophyll A ◽  
Early Stage ◽  
Chlorophyll Concentration ◽  
C4 Plants ◽  
Growth Responses ◽  
Amaranthus Tricolor ◽  
Sodium Deficiency ◽  
Group I ◽  
Chloris Gayana

Methods for the determination of chlorophyll were compared in Amaranthus tricolor, Kochia childsii and Chloris gayana. From sequential extraction data, 96% ethanol appeared to be more efficient than 80% acetone in extracting chlorophyll from these plants. The chlorophyll a/b ratio was significantly lower in sodium-deficient compared to normal C4 plants. Of the group I elements, only sodium, irrespective of the salt supplied to deficient cultures, restored the chlorophyll a/b ratios to the value observed in normal plants. The concentration of sodium required to increase the chlorophyll a/b ratio in leaves of sodium-deficient plants was similar to that required to bring about the growth responses. The increase of the chlorophyll a/b ratio occurred at an early stage during recovery from sodium deficiency preceding the increase in chlorophyll concentration and the growth response. It is therefore likely that the low chlorophyll a/b ratio may be intrinsically associated with the condition of sodium deficiency.

Chlorophyll a/b Ratios and Photosystem Activity of Mesophyll and Bundle Sheath Fractions From Sodium-Deficient C4 Plants

1989 ◽  
Vol 16 (6) ◽  
pp. 449 ◽  
Author(s):  
M Johnston ◽  
CPL Grof ◽  
PF Brownell
Keyword(s):  
Photosystem Ii ◽  
Chlorophyll A ◽  
Photosystem I ◽  
Bundle Sheath ◽  
C4 Plants ◽  
Amaranthus Tricolor ◽  
Chloris Gayana ◽  
Photosystem Ii Activity

Significantly lower chlorophyll a/b ratios were observed in mesophyll thylakoids extracted from sodium-deficient than normal C4 plants of Amaranthus tricolor, Chloris gayana and Kochia childsii. The chlorophyll a/b ratio of bundle sheath thylakoids was not generally as significantly affected by sodium nutrition as the mesophyll thylakoids. The lower chlorophyll a/b ratio in mesophyll thylakoids of sodium-deficient plants was associated with a lower photosystem II activity. The effect of sodium nutrition on photosystem I activity in the mesophyll thylakoids was dependent upon the species. Although no effect of sodium nutrition on photosystem I was observed in mesophyll thylakoids of K. childsii, photosystem I activity was greater in sodium-deficient than normal plants in A. tricolor. The photosystem II and I activities of the bundle sheath thylakoids were not affected by sodium nutrition in either K. childsii or A. tricolor.

The Effect of Sodium Nutrition on the Pool Sizes of Intermediates of the C Photosynthetic Pathway

1988 ◽  
Vol 15 (6) ◽  
pp. 749 ◽  
Author(s):  
M Johnston ◽  
CPL Grof ◽  
PF Brownell
Keyword(s):  
Direct Application ◽  
Photosynthetic Pathway ◽  
Growth Responses ◽  
Amaranthus Tricolor ◽  
Mesophyll Cells ◽  
Sodium Deficiency ◽  
C4 Species ◽  
C3 Species ◽  
Chloris Gayana ◽  
Group 1

Changes in C4 pathway intermediates in response to sodium nutrition, consistent with the hypothesis that there is a limitation in the conversion of pyruvate to phosphoenolpyruvate (PEP) in the mesophyll cells in sodium-deficiency, were observed in the C4 species, Kochia childsii, Chloris gayana, Amaranthus edulis, Amaranthus tricolor and Atriplex spongiosa. In the C3 species Lycopersicon esculentum (tomato), no differences were observed when grown with or without added sodium. Of the group 1 elements, only sodium, irrespective of the salt supplied to deficient cultures, effected these changes in the C4 species. In the light, concentrations of aspartate, PEP and 3-phosphoglycerate (3-PGA) were lower and those of pyruvate and alanine were greater in sodium-deficient than normal plants. In the dark, concentrations of aspartate, pyruvate, alanine, PEP and 3-PGA were similar in sodium-deficient and normal plants. In the C4 species Atriplex spongiosa, the concentration of sodium required to bring about these changes corresponded to that required for growth responses. Rapid increases in the concentrations of malate, PEP and 3-PGA and decreases in pyruvate and alanine were observed following the direct application of sodium to leaves.

Characterisation of Leaf Fluorescence of Sodium-Deficient C4 Plants: Kinetics of Emissions From Whole Leaves and Fluorescence Properties of Isolated Thylakoids

1989 ◽  
Vol 16 (6) ◽  
pp. 459 ◽  
Author(s):  
CPL Grof ◽  
DBC Richards ◽  
M Johnston ◽  
PF Brownell
Keyword(s):  
Light Harvesting ◽  
Polynomial Function ◽  
Bundle Sheath ◽  
C4 Plants ◽  
Degree Polynomial ◽  
Amaranthus Tricolor ◽  
Fluorescence Kinetics ◽  
C4 Species ◽  
Chloris Gayana ◽  
Kinetics Of

Examination of whole-leaf fluorescence kinetics by means of a second-degree polynomial function showed a decrease in the rate of the rise from Fd to Fp in sodium-deficient compared with normal leaves of the C4 species Kochia childsii and Amaranthus tricolor. This suggests a decreased efficiency in light harvesting and/or utilisation in sodium-deficient plants. Fluorescence ratios (Fv/Fo) of separated mesophyll and bundle sheath thylakoids were both lower from leaves of sodium-deficient compared with normal plants of K. childsii, Chloris gayana, A. edulis and A. tricolor.

In vivo Chlorophyll a Fluorescence in Sodium-Deficient C4 Plants

1986 ◽  
Vol 13 (5) ◽  
pp. 589
Author(s):  
CPL Grof ◽  
M Johnston ◽  
PF Bronwell
Keyword(s):  
Chlorophyll A ◽  
Chlorophyll A Fluorescence ◽  
Growth Response ◽  
Leaf Tissue ◽  
C4 Plants ◽  
Culture Solution ◽  
Fluorescence Ratio ◽  
Group I ◽  
Parallel Increase

The fluorescence ratio (Fp-FI)/FI gave a consistent value when leaf tissue weights greater than 0.1 g were used and was lower in sodium-deficient than normal C4 plants. Of the group I elements only sodium, irrespective of the salt supplied to deficient cultures, increased the fluorescence ratio to that observed in normal plants. The concentration of sodium required to increase the fluorescence ratio corresponded to the concentration of sodium required to bring about a growth response in Kochia childsii. The application of sodium, either directly onto the leaves or via the culture solution, brought about a rapid and parallel increase in the chlorophyll a/b ratio and the fluorescence ratio in K. childsii.

Responses to Ambient CO2 Concentrations by Sodium-Deficient C4 Plants

1984 ◽  
Vol 11 (3) ◽  
pp. 137 ◽  
Author(s):  
M Johnston ◽  
CPL Grof ◽  
PF Brownell
Keyword(s):  
Co2 Concentration ◽  
C4 Plants ◽  
C3 Plants ◽  
Amaranthus Tricolor ◽  
Sodium Deficiency ◽  
Low Co2 ◽  
C3 Species ◽  
High Co2 Concentration ◽  
Increased Co2 Concentration ◽  
Ambient Co2

The signs of sodium deficiency in the C4 species Amaranthus tricolor and Atriplex spongiosa were alleviated when the species were grown in conditions of high CO2 concentration (1 500 �l CO2 1-1). In experiments in which A. tricolor was grown in conditions of low CO2 concentration (15 �l CO2 1-1), the signs of sodium deficiency were accentuated. The sodium-deficient plants resembled the C3 species Atriplex hastata and tomato in their response to the CO2 treatments. Growth and chlorophyll concentrations increased in both the sodium-deficient C4 plants and the C3 plants when they were grown in conditions of increased CO2 concentration. Sodium-sufficient C4 plants were relatively unaffected by the CO2 treatments.

Authenticity of speciality sugars – Determination of carbohydrate source and geographical origin of invert sugar syrup and burnt sugar syrup

2017 ◽  
pp. 87-91
Author(s):  
Andreas G. Degenhardt ◽  
Elke Jansen ◽  
Timo, J. Koch
Keyword(s):  
Geographical Origin ◽  
Hydrological Cycle ◽  
Isotope Ratios ◽  
Saccharum Officinarum ◽  
Invert Sugar ◽  
C4 Plants ◽  
Yeast Fermentation ◽  
Carbohydrate Source ◽  
Sugar Syrup

Modern instrumental analytical methods for the determination of 13C/12C ratios are established to differentiate between metabolic products of C3 and C4 plants. Differentiation and identification of sucrose from pure beet (Beta vulgaris) and pure cane (Saccharum officinarum) are possible without doubt. Influenced by the worldwide hydrological cycle the determination of the isotope ratios of 2H/1H and 18O/16O as well as their variations provide information about geographical origin. Using samples of selected crystal cane sugar (CCS) with known origin, invert sugar syrups (ISS) as well as burnt sugar syrups (BSS) produced therefrom, the authenticity was determined. The speciality sugars ISS and BSS which were made from CCS could be identified as carbohydrates of C4 plants by using 13C/12C Isotope-Ratio Mass Spectrometry (IRMS). In combination with yeast fermentation of ISS and sugar separation from BSS and fermentation into ethanol as well as knowledge about production water, the C2-H/O isotope ratios of ethanol can theoretically determine the geographical origin of the sugars.

scholarly journals Evolution of Antennapedia-Class Homeobox Genes

Genetics ◽  
1996 ◽  
Vol 142 (1) ◽  
pp. 295-303 ◽  
Author(s):  
Jianzhi Zhang ◽  
Masatoshi Nei
Keyword(s):  
Gene Expression ◽  
Homeobox Genes ◽  
Amino Acid Sequences ◽  
Gene Arrangement ◽  
Gene Duplications ◽  
Group I ◽  
Group 3 ◽  
Group Ii ◽  
Anterior Posterior

Antennapedia (Antp)-class homeobox genes are involved in the determination of pattern formation along the anterior-posterior axis of the animal embryo. A phylogenetic analysis of Antp-class homeodomains of the nematode, Drosophila, amphioxus, mouse, and human indicates that the 13 cognate group genes of this gene family can be divided into two major groups, i.e., groups I and II. Group I genes can further be divided into subgroups A (cognate groups 1–2), B (cognate group 3), and C (cognate groups 4–8), and group II genes can be divided into subgroups D (cognate groups 9–10) and E (cognate groups 11–13), though this classification is somewhat ambiguous. Evolutionary distances among different amino acid sequences suggest that the divergence between group I and group II genes occurred ∼1000 million years (MY) ago, and the five different subgroups were formed by ∼600 MY ago, probably before the divergence of Pseudocoelomates (e.g., nematodes) and Coelomates (e.g., insects and chordates). Our results show that the genes that are phylogenetically close are also closely located in the chromosome, suggesting that the colinearity between the gene expression and gene arrangement was generated by successive tandem gene duplications and that the gene arrangement has been maintained by some sort of selection.

scholarly journals The expression of microRNA-331-3p and microRNA-23b3 in Egyptian patients with early-stage hepatocellular carcinoma in hepatitis C-related liver cirrhosis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Reham A. Aboelwafa ◽  
Walid Ismail Ellakany ◽  
Marwa A. Gamaleldin ◽  
Marwa A. Saad
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cirrhosis ◽  
Hepatitis C ◽  
Early Stage ◽  
Group Iii ◽  
Real Time Quantitative Pcr ◽  
Early Stages ◽  
Group I ◽  
Egyptian Patients ◽  
Cirrhotic Patients

Abstract Background Hepatocellular carcinoma and hepatitis C are strongly associated. The current work aimed to study the expression levels of microRNA-331-3p and microRNA-23b-3p as propable biomarkers for detecting liver cancer (HCC) at its early stages in patients with HCV-related liver cirrhosis. The current prospective study included two hundred participants, divided into three groups: group I, 100 patients with HCV-related liver cirrhosis; group II, 50 HCC patients at early stages; and group III, 50 apparentlyhealthy controls. All patients had routine laboratory workup and ultrasound hepatic assessment. Values of microRNA-331-3p and microRNA-23b-3p were measured by real-time quantitative PCR. Results Levels of miR-331-3p were significantly higher in HCC patients than in cirrhotic patients and controls (p < 0.001), while levels of miR-23b-3p were significantly lower in HCC patients compared to cirrhotics and controls (p < 0.001). ROC curve revealed that miR-23b-3p had 80% sensitivity and 74% specificity, miR-331-3p had 66% sensitivity and 61% specificity, and AFP had 64% sensitivity and 61% specificity of 61% in discrimination between HCC patients from controls. Conclusion Serum miR-23b-3p is a more effective predictor than miR-331-3p and AFP for the development of hepatocellular carcinoma in hepatitis C (HCV)-related cirrhotic patients.

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