Identification of pollen donors for the sweet cherry cultivars ‘Stella’ and ‘Summit’ by isozyme analysis

1999 ◽  
Vol 39 (4) ◽  
pp. 473 ◽  
Author(s):  
B. Brant ◽  
A. R. Granger ◽  
J. Witherspoon ◽  
G. G. Collins
Keyword(s):  
Sweet Cherry ◽  
Mixed Block ◽  
Glucose Phosphate Isomerase ◽  
Shikimate Dehydrogenase ◽  
Glutamate Oxaloacetate Transaminase ◽  
Full Bloom ◽  
Phosphogluconate Dehydrogenase ◽  
Glucose Phosphate ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Sweet Cherry Cultivars

Pollinisers of the sweet cherry cultivars ‘Stella’ and ‘Summit’ were determined by analysing isozymes of glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6-PGD), glucose phosphate isomerase (GPI), isocitrate dehydrogenase (IDH), shikimate dehydrogenase (SKDH), fructose-1,6-diphosphatase (FDP), and glutamate oxaloacetate transaminase (GOT) in their embryos. Possible polliniser cultivars were selected on the basis of similar full bloom dates and orchard position in regard to Stella or Summit. Examination of the ratios for the segregation of isozymes showed that Summit was predominantly pollinated by Stella, and Stella by Van. In the same orchard, but in a different season, the main polliniser for Stella was found to be Venus, and 29% of Stella embryos resulted from selfing. Thus the effectiveness of cherry pollinisers depends on overlapping flowering dates, which can vary from year to year, and, for a self-fertile cultivar in a mixed block, cross-pollination predominates over selfing.

Comparison of human and murine isolates of Schistosoma mansoni from Richard-Toll, Senegal, by isoelectric focusing

1997 ◽  
Vol 71 (2) ◽  
pp. 175-181 ◽  
Author(s):  
M. Sène ◽  
P. Brémond ◽  
J.P. Hervé ◽  
V.R. Southgate ◽  
B. Sellin ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Schistosoma Mansoni ◽  
Isoelectric Focusing ◽  
Glucose Phosphate Isomerase ◽  
Glucose Phosphate ◽  
Enzyme Systems ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Arvicanthis Niloticus

AbstractStudies on human and murine isolates of Schistosoma mansoni, from Richard-Toll, Senegal, were carried out by isoelectric focusing in polyacrylamide gels. Seven enzyme systems; lactate dehydrogenase (LDH), malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G6PD), acid phosphatase (AcP), hexokinase (HK), glucose phosphate isomerase (GPI), and phosphoglucomutase (PGM), were used to compare the two isolates. All systems tested, apart from LDH, were found to be polymorphic for both isolates. Interestingly, one phenotype is more frequent than the remainder. The results show that there is no significant genetic variation between the S. mansoni isolates from man and the rodents, Arvicanthis niloticus and Mastomys huberti.

Isozyme variation and inheritance in blueberry

Genome ◽  
1988 ◽  
Vol 30 (5) ◽  
pp. 776-781 ◽  
Author(s):  
Nicholi Vorsa ◽  
Paul S. Manos ◽  
Maria I. van Heemstra
Keyword(s):  
Single Gene ◽  
Leaf Tissue ◽  
Mendelian Inheritance ◽  
Shikimate Dehydrogenase ◽  
Banding Patterns ◽  
Phosphogluconate Dehydrogenase ◽  
Tissue Extracts ◽  
Isozyme Polymorphism ◽  
Taxonomic Studies ◽  
Glucose 6 Phosphate Dehydrogenase

Leaf tissue extracts from diploid, tetraploid, and hexaploid species of blueberry, Vaccinium section Cyanococcus, were electrophoretically analyzed for isozyme polymorphism in 12 enzyme systems. Aldolase, shikimate dehydrogenase, triose phosphate isomerase, glucose-6-phosphate dehydrogenase, phosphoglucomutase, aconitase, alcohol dehydrogenase, and aspartate aminotransferase showed activity, but banding was not clear. Four enzymes, malate dehydrogenase (MDH), phosphoglucose isomerase (PGI), 6-phosphogluconate dehydrogenase (6-PGD), and isocitrate dehydrogenase (IDH), exhibited interpretable banding patterns. Two loci were apparent for MDH, PGI, and 6-PGD and one for IDH. Polymorphism was detected at Mdh-2, Pgi-2, 6-Pgd-2, and Idh. Three allozymes were found at Mdh-2 and Idh and at least four at Pgi-2 and 6-Pgd-2. Allozyme segregation ratios observed in progeny of controlled diploid crosses supported single-gene Mendelian inheritance. Banding patterns of all allozymes indicated a dimeric structure for these four enzymes. It appears that all alleles at multiallelic loci are expressed in polyploids. Preliminary data suggest that allozyme analyses may be useful in taxonomic studies in blueberry.Key words: Vaccinium, allozymes, inheritance, electrophoresis.

scholarly journals Linkage Analysis of Ten Isozyme Genes in F1 Segregating Almond Progenies

1994 ◽  
Vol 119 (2) ◽  
pp. 339-344 ◽  
Author(s):  
Pere Arús ◽  
Carmen Olarte ◽  
Miguel Romero ◽  
Francisco Vargas
Keyword(s):  
Leucine Aminopeptidase ◽  
Recombination Fraction ◽  
Joint Estimation ◽  
Linkage Data ◽  
Linkage Groups ◽  
Shikimate Dehydrogenase ◽  
Prunus Amygdalus ◽  
Phosphogluconate Dehydrogenase ◽  
Glucose Phosphate ◽  
Enzyme Systems

Ten isozyme genes were studied after analyzing the variability of eight enzyme systems—glucose phosphate isomerase (GPI), phosphoglucomutase (PGM), aspartate aminotransferase (AAT), leucine aminopeptidase (LAP), 6-phosphogluconate dehydrogenase (6PGD), isocitrate dehydrogenase (IDH), shikimate dehydrogenase (SDH), and aconitase (ACO)—in the progeny of five crosses among almond [Prunus amygdalus Batsch, syn. P. dulcis (Miller) D. A. Webb] cultivars. Six of these loci were found to be located in two linkage groups, one containing four loci (Pgm-2, Gpi-2, Aat-2, and Lap-1) and two more in the other (Idh-2 and Aat-1). Genetic configurations of pairs of loci specific to segregating F1 progeny of crosses between heterozygous parents were found in our data, for which we derived the estimate of the recombination fraction and its variance. Linkage data for the gene pairs that could be estimated in various crosses were used to obtain a joint estimation of the recombination fraction.

scholarly journals Detection of Glucose-Phosphate Isomerase Deficiency by a Screening Procedure

Blood ◽  
1972 ◽  
Vol 39 (5) ◽  
pp. 685-687 ◽  
Author(s):  
Karl-Georg Blume ◽  
Ernest Beutler
Keyword(s):  
Rapid Detection ◽  
Screening Procedure ◽  
Red Cell ◽  
Glucose Phosphate Isomerase ◽  
Glucose Phosphate ◽  
Dehydrogenase Reaction ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Cell Glucose

Abstract A method for rapid detection of red cell glucose-phosphate isomerase deficiency is described. The procedure is based on the appearance of fluorescence, caused by TPNH, that is generated in the linked glucose-phosphate isomerase/glucose-6-phosphate dehydrogenase reaction.

Enzyme variation in Eimeria species of the chicken

Parasitology ◽  
1975 ◽  
Vol 71 (3) ◽  
pp. 369-376 ◽  
Author(s):  
M. W. Shirley
Keyword(s):  
Species Differences ◽  
Eimeria Species ◽  
Starch Gel Electrophoresis ◽  
Phosphogluconate Dehydrogenase ◽  
Glucose Phosphate ◽  
Parasite Stage ◽  
Starch Gel ◽  
Biochemical Identification ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
First Time

A method for the biochemical identification of protozoa belonging to the genus Eimeria is described for the first time. Starch gel electrophoresis of the enzymes lactate dehydrogenase, glucose phosphate isomerase, 6-phosphogluconate dehydrogenase and glucose-6-phosphate dehydrogenase from parasite extracts revealed both intra- and inter-species differences when 11 strains representative of 6 species of Eimeria were examined. Oocysts were the most accessible parasite stage for investigation but sporozoites and merozoites of an embryo-adapted strain of E. tenella were also examined for enzyme activity.

ELECTROPHORETIC STUDY OF ENZYMES FROM A GLOSSINA FUSCIPES FUSCIPES NEWSTEAD POPULATION FROM WESTERN KENYA

1991 ◽  
Vol 123 (2) ◽  
pp. 295-298 ◽  
Author(s):  
G.F. Rajendram
Keyword(s):  
Lake Victoria ◽  
Xanthine Dehydrogenase ◽  
Autosomal Locus ◽  
Glucose Phosphate Isomerase ◽  
Electrophoretic Study ◽  
Western Kenya ◽  
Glossina Fuscipes Fuscipes ◽  
Glucose Phosphate ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Rusinga Island

AbstractEnzymes were investigated, by electrophoresis, in a population of Glossina fuscipes fuscipes Newstead collected from Rusinga Island in Lake Victoria, Western Kenya.The following enzymes were tested: glucose phosphate isomerase, glucose-6-phosphate dehydrogenase (G6PDH), hexokinase. isocitrate dehydrogenase (IDH), malate-dehydrogenase (MDH), phosphoglucomutase, and xanthine dehydrogenase (XDH).Single monomorphic bands were stained by the following enzymes apparently under the control of single loci: G6PDH, MDH, and XDH. The enzyme IDH showed two bands with very close mobilities and no variation among individuals in the population. Hence IDH was considered as representing a single locus. Glucose phosphate isomerase manifested three alleles and apparently six genotypes. Phosphoglucomutase manifested a double-banded pattern representing an autosomal locus.

scholarly journals Genetic characterization of a brangus-ibage cattle population: biochemical polymorphisms and reproductive efficiency

2000 ◽  
Vol 30 (5) ◽  
pp. 803-807
Author(s):  
Luiz Ernani Henkes ◽  
Lídia Gonzalez Papadopolis ◽  
Clara Sabina Steigleder ◽  
José Carlos Ferrugem Moraes ◽  
Tania de Azevedo Weimer
Keyword(s):  
Genetic Characterization ◽  
Blood Protein ◽  
Reproductive Efficiency ◽  
Phosphogluconate Dehydrogenase ◽  
Glucose Phosphate ◽  
Biochemical Polymorphisms ◽  
The Mean ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Good Agreement

Biochemical techniques were used to investigate the genetic variability in a Brangus-Ibage population by determining allele frequencies of 18 blood protein systems: Hemogloin beta-Chain (Hb), Albumin (Alb), Amylase (Am), Transferrin (Tf), Carbonic Anhydrase (CA), Ceruloplasmin (Cp), Malic Enzyme (ME), Diaphorase I and II (Dia I and Dia II), Slow Alpha 2 Macroglobulin (Ap), Acid Phosphatase (ACP), Esterase B and D (EstB and EstD), Phosphogluconate Dehydrogenase (PGD), Glucose-6-Phosphate Dehydrogenase (G-6-PD), Glucose-Phosphate-Isomerase (GPI), Superoxide Dismutase (SOD) and Glyoxalase I (GLO). The percentage of polymorphic loci were estimated at 0.27, the mean number of alleles was 1.33 and the mean heterozygosity was 0.07. There was a good agreement between expected and observed heterozygosity values. The population was in agreement with Hardy-Weinberg expectations in all systems. Reproductive records allowed to estimate three parameters of reproductive efficiency: mean age at first calving (1152.15 ± 166.60 days), mean calving interval (539.23 ± 124.10 days) and mean weight at first calving (391.02 ± 37.59kg). No relationship was found between reproductive efficiency and genetic systems.

Inheritance of needle tissue isozymes in Douglas-fir

1984 ◽  
Vol 26 (4) ◽  
pp. 459-468 ◽  
Author(s):  
D. B. Neale ◽  
J. C. Weber ◽  
W. T. Adams
Keyword(s):  
Glutamate Dehydrogenase ◽  
Pseudotsuga Menziesii ◽  
Isocitrate Dehydrogenase ◽  
Seed Orchard ◽  
Douglas Fir ◽  
Glutamate Oxaloacetate Transaminase ◽  
Electrophoretic Variants ◽  
Phosphogluconate Dehydrogenase ◽  
Enzyme Systems ◽  
Glucose 6 Phosphate Dehydrogenase

Methods for resolving electrophoretic variants from extracts of needle tissue of coastal Douglas-fir (Pseudotsuga menziesii var. menziesii (Mirb.) Franco) are described, and the inheritance of 12 of at least 15 loci that control allozymes from 11 enzyme systems are established. Evidence for the inheritance of allozyme variants was obtained in three ways: (i) comparison in seed orchard clones of allozyme genotypes determined from both megagametophyte and needle tissue; (ii) analysis of segregating full-sib progenies of seed orchard clones; and (iii) comparison of needle allozyme pattern phenotypes to previously reported embryo phenotypes. Ten of the 12 loci (coding phosphoglucomutase, PGM(1) and PGM(2); glycerate dehydrogenase, GLYDH; phosphoglucose isomerase, PG1(2); glutamate dehydrogenase, GDH; glucose-6-phosphate dehydrogenase, G-6PD; 6-phosphogluconate dehydrogenase, 6-PGD(1); isocitrate dehydrogenase, IDH; diaphorase, DIA(2); malate dehydrogenase, MDH(1)) produce clear bands in seed tissue; however, glutamate oxaloacetate transaminase GOT(3) (N) was not found in seeds and shikimic dehydrogenase (SDH) could only be clearly resolved in needles (N). Several enzymes active in seed tissue could not be detected in needle tissues.Key words: Douglas-fir, needle tissue isozymes, inheritance.

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