ELECTROPHORETIC STUDY OF ENZYMES FROM A GLOSSINA FUSCIPES FUSCIPES NEWSTEAD POPULATION FROM WESTERN KENYA

1991 ◽  
Vol 123 (2) ◽  
pp. 295-298 ◽  
Author(s):  
G.F. Rajendram

AbstractEnzymes were investigated, by electrophoresis, in a population of Glossina fuscipes fuscipes Newstead collected from Rusinga Island in Lake Victoria, Western Kenya.The following enzymes were tested: glucose phosphate isomerase, glucose-6-phosphate dehydrogenase (G6PDH), hexokinase. isocitrate dehydrogenase (IDH), malate-dehydrogenase (MDH), phosphoglucomutase, and xanthine dehydrogenase (XDH).Single monomorphic bands were stained by the following enzymes apparently under the control of single loci: G6PDH, MDH, and XDH. The enzyme IDH showed two bands with very close mobilities and no variation among individuals in the population. Hence IDH was considered as representing a single locus. Glucose phosphate isomerase manifested three alleles and apparently six genotypes. Phosphoglucomutase manifested a double-banded pattern representing an autosomal locus.

1997 ◽  
Vol 71 (2) ◽  
pp. 175-181 ◽  
Author(s):  
M. Sène ◽  
P. Brémond ◽  
J.P. Hervé ◽  
V.R. Southgate ◽  
B. Sellin ◽  
...  

AbstractStudies on human and murine isolates of Schistosoma mansoni, from Richard-Toll, Senegal, were carried out by isoelectric focusing in polyacrylamide gels. Seven enzyme systems; lactate dehydrogenase (LDH), malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G6PD), acid phosphatase (AcP), hexokinase (HK), glucose phosphate isomerase (GPI), and phosphoglucomutase (PGM), were used to compare the two isolates. All systems tested, apart from LDH, were found to be polymorphic for both isolates. Interestingly, one phenotype is more frequent than the remainder. The results show that there is no significant genetic variation between the S. mansoni isolates from man and the rodents, Arvicanthis niloticus and Mastomys huberti.


Blood ◽  
1972 ◽  
Vol 39 (5) ◽  
pp. 685-687 ◽  
Author(s):  
Karl-Georg Blume ◽  
Ernest Beutler

Abstract A method for rapid detection of red cell glucose-phosphate isomerase deficiency is described. The procedure is based on the appearance of fluorescence, caused by TPNH, that is generated in the linked glucose-phosphate isomerase/glucose-6-phosphate dehydrogenase reaction.


1999 ◽  
Vol 39 (4) ◽  
pp. 473 ◽  
Author(s):  
B. Brant ◽  
A. R. Granger ◽  
J. Witherspoon ◽  
G. G. Collins

Pollinisers of the sweet cherry cultivars ‘Stella’ and ‘Summit’ were determined by analysing isozymes of glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6-PGD), glucose phosphate isomerase (GPI), isocitrate dehydrogenase (IDH), shikimate dehydrogenase (SKDH), fructose-1,6-diphosphatase (FDP), and glutamate oxaloacetate transaminase (GOT) in their embryos. Possible polliniser cultivars were selected on the basis of similar full bloom dates and orchard position in regard to Stella or Summit. Examination of the ratios for the segregation of isozymes showed that Summit was predominantly pollinated by Stella, and Stella by Van. In the same orchard, but in a different season, the main polliniser for Stella was found to be Venus, and 29% of Stella embryos resulted from selfing. Thus the effectiveness of cherry pollinisers depends on overlapping flowering dates, which can vary from year to year, and, for a self-fertile cultivar in a mixed block, cross-pollination predominates over selfing.


2018 ◽  
Author(s):  
Cindy L. Hendry ◽  
◽  
Kirsten E. Jenkins ◽  
Peter Li ◽  
Lauren A. Michel ◽  
...  

Genomics ◽  
1990 ◽  
Vol 7 (4) ◽  
pp. 638-643 ◽  
Author(s):  
James I.H. Walker ◽  
Pelin Faik ◽  
Michael J. Morgan

1987 ◽  
Vol 316 (5) ◽  
pp. 258-261 ◽  
Author(s):  
Yaddanapudi Ravindranath ◽  
Donald E. Paglia ◽  
Indira Warrier ◽  
William Valentine ◽  
Misae Nakatani ◽  
...  

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