Screening wild progenitors of wheat for salinity stress at early stages of plant growth: insight into potential sources of variability for salinity adaptation in wheat

2018 ◽  
Vol 69 (7) ◽  
pp. 649 ◽  
Author(s):  
Jafar Ahmadi ◽  
Alireza Pour-Aboughadareh ◽  
Sedigheh Fabriki-Ourang ◽  
Ali-Ashraf Mehrabi ◽  
Kadambot H. M. Siddique
Keyword(s):  
Salinity Stress ◽  
Principal Component ◽  
Wild Relatives ◽  
Aegilops Speltoides ◽  
Tolerance Mechanisms ◽  
B Genome ◽  
Oxidative Balance ◽  
Triticum Boeoticum ◽  
Extensive Collection ◽  
Wild Progenitors

Wild relatives of wheat have served as a pool of genetic variation for understanding salinity tolerance mechanisms. Two separate experiments were performed to evaluate the natural diversity in root and shoot Na+ exclusion and K+ accumulation, and the activity of four antioxidant enzymes within an extensive collection of ancestral wheat accessions. In the initial screening experiment, salinity stress (300 mm NaCl) significantly increased Na+ concentration in roots and leaves and led to a significant decline in root and shoot fresh weights, dry weights, and K+ contents. Principal component analysis of the 181 accessions and 12 species identified three first components accounted for 63.47% and 78.55% of the variation under salinity stress. We identified 12 accessions of each species with superior tolerance to salinity for further assessment of their antioxidant defence systems in response to salinity. Both mild (250 mm NaCl) and severe (350 mm NaCl) levels of salinity significantly increased activities of four enzymes, indicating an enhanced antioxidant-scavenging system for minimising the damaging effects of H2O2. Some of the wild relatives—Aegilops speltoides (putative B genome), Ae. caudata (C genome), Ae. cylindrica (DC genome) and Triticum boeoticum (Ab genome)—responded to salinity stress by increasing antioxidants as the dominant mechanism to retain oxidative balance in cells. Further evaluation of salt-tolerance mechanisms in these superior wild relatives will help us to understand the potential of wheat progenitors in the development of more salt-tolerant varieties.

Chromosome structural changes and their role in the evolution of tetraploid wheats

Genome ◽  
1989 ◽  
Vol 32 (2) ◽  
pp. 257-261 ◽  
Author(s):  
Kaz. Noda ◽  
Ge Koulin
Keyword(s):  
Key Words ◽  
Structural Changes ◽  
Triticum Dicoccoides ◽  
Aegilops Speltoides ◽  
Banding Patterns ◽  
Tetraploid Wheats ◽  
B Genome ◽  
Triticum Boeoticum

The possibility that chromosomal interchanges present in the Timopheevi group (AAGG) are responsible for the differentiation of two different groups of tetraploid wheats, the Emmer group (AABB) and the Timopheevi (AAGG) group, has been examined. Chromosomes of Triticum dicoccoides (2n = 28, AABB) and six interchange types of the Timopheevi group (2n = 28, AAGG) were studied by N-banding. The banding patterns of the G genome were distinct from those of the B genome. The banding patterns of the G genome chromosomes of the interchange types were similar to the basic banding patterns of the G genome except those of interchanged chromosomes. No intermediate banding patterns between the B and G genomes were observed. These interchanges might have occurred after establishment of the basic karyotype of the G genome and would not be related to the differentiation of AABB and AAGG from a progenitor tetraploid, such as amphidiploid (AASS) between Aegilops speltoides (SS) and Triticum boeoticum (AA).Key words: tetraploid wheats, N-banding, interchange.

scholarly journals Effects of NaCl on protein profiles of tetraploid and hexaploid wheat species and their diploid wild progenitors

2008 ◽  
Vol 54 (No. 6) ◽  
pp. 227-233 ◽  
Author(s):  
M. Yıldız ◽  
H. Terzi
Keyword(s):  
Salt Stress ◽  
Nacl Stress ◽  
Control Treatment ◽  
Aegilops Speltoides ◽  
Protein Profiles ◽  
Wheat Species ◽  
Tolerance Mechanisms ◽  
Leaf Tissues ◽  
2D Gel ◽  
Wild Progenitors

The soluble proteins extracted from the first leaf tissues of cultivated tetraploid (<I>Triticum durum</I> Desf., genome <I>AB</I>) and hexaploid (<I>T. aestivum</I> L., genome <I>ABD</I>) wheat species and their diploid wild progenitors [<I>T. monococcum</I> L. (<I>A), Aegilops speltoides</I> Tausch (<I>B</I>), and <I>Aegilops tauschi</I>i Cosson (<I>D</I>)] exposed to 100 mmol/l NaCl stress were separated by two-dimensional (2D) gel electrophoresis. There was no newly synthesized protein in the NaCl treatment compared to the control treatment in all species. However, protein profiles showed some differences among species. Most of these proteins had acidic character; their isoelectric point (pI = pH − acidity of proteins) ranged between 5.1–6.9 and low-mol weight (LMW) between 20.3–30.6 kDa. Salt stress caused some proteins to increase or decrease. In the same MW and pI, 11 LMW and 3 intermediate-mol weight (IMW, 34.8–35.4 kDa) proteins increased and/or decreased in amounts were common between at least two species. The remarkable changes in <I>Ae. speltoides</I> were detected as decreases or losses in protein profiles. As a result of salt stress, all the remarkable changes in <I>T. durum</I> were detected as the increases in proteins. However, some proteins increased in <I>T. aestivum, T. monococcum</I> and <I>Ae. tauschii</I>. It is suggested that an increase in the amount of the proteins may lead to an increase in the tolerance mechanisms towards NaCl salinity of wheat species which has A and/or genome(s).

scholarly journals Exogenous Putrescine Enhances Salt Tolerance and Ginsenosides Content in Korean Ginseng (Panax ginseng Meyer) Sprouts

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1313
Author(s):  
Md. Jahirul Islam ◽  
Byeong Ryeol Ryu ◽  
Md. Obyedul Kalam Azad ◽  
Md. Hafizur Rahman ◽  
Md. Soyel Rana ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Salinity Stress ◽  
Soluble Sugar ◽  
Principal Component ◽  
Total Soluble Protein ◽  
Soluble Carbohydrate ◽  
Guaiacol Peroxidase ◽  
Salinity Treatment ◽  
Total Soluble Sugar ◽  
Biochemical Processes

The effect of exogenously applied putrescine (Put) on salt stress tolerance was investigated in Panax ginseng. Thirty-day-old ginseng sprouts were grown in salinized nutrient solution (150 mM NaCl) for five days, while the control sprouts were grown in nutrients solution. Putrescine (0.3, 0.6, and 0.9 mM) was sprayed on the plants once at the onset of salinity treatment, whereas control plants were sprayed with water only. Ginseng seedlings tested under salinity exhibited reduced plant growth and biomass production, which was directly interlinked with reduced chlorophyll and chlorophyll fluorescence due to higher reactive oxygen species (hydrogen peroxide; H2O2) and lipid peroxidation (malondialdehyde; MDA) production. Application of Put enhanced accumulation of proline, total soluble carbohydrate, total soluble sugar and total soluble protein. At the same time, activities of antioxidant enzymes like superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase in leaves, stems, and roots of ginseng seedlings were increased. Such modulation of physio-biochemical processes reduced the level of H2O2 and MDA, which indicates a successful adaptation of ginseng seedlings to salinity stress. Moreover, protopanaxadiol (PPD) ginsenosides enhanced by both salinity stress and exogenous Put treatment. On the other hand, protopanaxatriol (PPT) ginsenosides enhanced in roots and reduced in leaves and stems under salinity stress condition. In contrast, they enhanced by exogenous Put application in all parts of the plants for most cases, also evidenced by principal component analysis. Collectively, our findings provide an important prospect for the use of Put in modulating salinity tolerance and ginsenosides content in ginseng sprouts.

A Centromeric Tandem Repeat Family Originating From a Part of Ty3/gypsy-Retroelement in Wheat and Its Relatives

Genetics ◽  
2003 ◽  
Vol 164 (2) ◽  
pp. 665-672 ◽  
Author(s):  
Zhi-Jun Cheng ◽  
Minoru Murata
Keyword(s):  
In Situ Hybridization ◽  
Tandem Repeats ◽  
Diploid Species ◽  
Upstream Region ◽  
Aegilops Speltoides ◽  
Repeat Family ◽  
B Genome ◽  
Intervening Sequences ◽  
Wild Diploid Species

AbstractFrom a wild diploid species that is a relative of wheat, Aegilops speltoides, a 301-bp repeat containing 16 copies of a CAA microsatellite was isolated. Southern blot and fluorescence in situ hybridization revealed that ∼250 bp of the sequence is tandemly arrayed at the centromere regions of A- and B-genome chromosomes of common wheat and rye chromosomes. Although the DNA sequence of this 250-bp repeat showed no notable homology in the databases, the flanking or intervening sequences between the repeats showed high homologies (&gt;82%) to two separate sequences of the gag gene and its upstream region in cereba, a Ty3/gypsy-like retroelement of Hordeum vulgare. Since the amino acid sequence deduced from the 250 bp with seven CAAs showed some similarity (∼53%) to that of the gag gene, we concluded that the 250-bp repeats had also originated from the cereba-like retroelements in diploid wheat such as Ae. speltoides and had formed tandem arrays, whereas the 300-bp repeats were dispersed as a part of cereba-like retroelements. This suggests that some tandem repeats localized at the centromeric regions of cereals and other plant species originated from parts of retrotransposons.

scholarly journals Contribution of Wild Relatives to Durum Wheat (Triticum turgidum subsp. durum) Yield Stability across Contrasted Environments

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1992
Author(s):  
Hafid Aberkane ◽  
Ahmed Amri ◽  
Bouchra Belkadi ◽  
Abdelkarim Filali-Maltouf ◽  
Jan Valkoun ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Triticum Turgidum ◽  
Wild Relatives ◽  
Yield Stability ◽  
Yield Potential ◽  
High Yield ◽  
Interspecific Crosses ◽  
Aegilops Speltoides ◽  
Recurrent Parent ◽  
Environment Interaction

Durum wheat (Triticum turgidum subsp. durum) is mostly grown in Mediterranean type environments, characterized by unpredictable rainfall amounts and distribution, heat stress, and prevalence of major diseases and pests, all to be exacerbated with climate change. Pre-breeding efforts transgressing adaptive genes from wild relatives need to be strengthened to overcome these abiotic and biotic challenges. In this study, we evaluated the yield stability of 67 lines issued from interspecific crosses of Cham5 and Haurani with Triticum dicoccoides, T. agilopoides, T. urartu, and Aegilops speltoides, grown under 15 contrasting rainfed and irrigated environments in Morocco, and heat-prone conditions in Sudan. Yield stability was assessed using parametric (univariate (e.g., Bi, S2di, Pi etc) and multivariate (ASV, SIPC)) and non-parametric (Si1, Si2, Si3 and Si6) approaches. The combined analysis of variance showed the highly significant effects of genotypes, environments, and genotype-by-environment interaction (GEI). The environments varied in yield (1370–6468 kg/ha), heritability (0.08–0.9), and in their contribution to the GEI. Several lines derived from the four wild parents combined productivity and stability, making them suitable for unpredictable climatic conditions. A significant advantage in yield and stability was observed in Haurani derivatives compared to their recurrent parent. Furthermore, no yield penalty was observed in many of Cham5 derivatives; they had improved yield under unfavorable environments while maintaining the high yield potential from the recurrent parent (e.g., 142,026 and 142,074). It was found that a limited number of backcrosses can produce high yielding/stable germplasm while increasing diversity in a breeding pipeline. Comparing different stability approaches showed that some of them can be used interchangeably; others can be complementary to combine broad adaption with higher yield.

Characterization of six wheat × Thinopyrum intermedium derivatives by GISH, RFLP, and multicolor GISH

Genome ◽  
2003 ◽  
Vol 46 (3) ◽  
pp. 490-495 ◽  
Author(s):  
F P Han ◽  
G Fedak ◽  
A Benabdelmouna ◽  
K Armstrong ◽  
T Ouellet
Keyword(s):  
Genomic Dna ◽  
Aegilops Tauschii ◽  
Rflp Analysis ◽  
Aegilops Speltoides ◽  
Thinopyrum Intermedium ◽  
B Genome ◽  
A Genome ◽  
Genetic Stocks ◽  
Disomic Addition Lines ◽  
Thinopyrum Elongatum

Restriction fragment length polymorphism (RFLP) analysis and multicolor genomic in situ hybridization (GISH) are useful tools to precisely characterize genetic stocks derived from crosses of wheat (Triticum aestivum) with Thinopyrum intermedium and Thinopyrum elongatum. The wheat × Th. intermedium derived stocks designated Z1, Z2, Z3, Z4, Z5, and Z6 were initially screened by multicolor GISH using Aegilops speltoides genomic DNA for blocking and various combinations of genomic DNA from Th. intermedium, Triticum urartu, and Aegilops tauschii for probes. The probing (GISH) results indicated that lines Z1 and Z3 were alien disomic addition lines with chromosome numbers of 2n = 44. Z2 was a substitution line in which chromosome 2D was substituted by a pair of Th. intermedium chromosomes; this was confirmed by RFLP and muticolour GISH. Z4 (2n = 44) contained two pairs of wheat – Th. intermedium translocated chromosomes; one pair involved A-genome chromosomes, the other involved D- and A-genome chromosomes. Z5 (2n = 44) contained one pair of wheat – Th. intermedium translocated chromosomes involving the D- and A-genome chromosomes of wheat. Z6 (2n = 44) contained one pair of chromosomes derived from Th. intermedium plus another pair of translocated chromosomes involving B-genome chromosomes of wheat. Line Z2 was of special interest because it has some resistance to infection by Fusarium graminearum.Key words: wheat, Thinopyrum intermedium, addition, substitution, and translocation lines, GISH, multicolor GISH, RFLP.

Biochemical data bearing on the origin of the B genome in the polyploid wheats

Genome ◽  
1990 ◽  
Vol 33 (3) ◽  
pp. 360-368 ◽  
Author(s):  
K. Kerby ◽  
J. Kuspira ◽  
B. L. Jones ◽  
G. L. Lookhart
Keyword(s):  
Amino Acid ◽  
B Chromosome ◽  
Amino Acid Sequences ◽  
Chromosome 1 ◽  
Aegilops Speltoides ◽  
B Genome ◽  
Aegilops Longissima ◽  
Aegilops Sharonensis ◽  
A Genome ◽  
Genome Donors

For many years each of the species Aegilops bicornis, Aegilops longissima, Aegilops searsii, Aegilops sharonensis, Aegilops speltoides, and Triticum urartu has been implicated as the donor of the B genome in the polyploid wheats. Biochemical and cytological data have revealed that T. urartu possesses a genome similar to that of T. monococcum, and therefore it may be the source of the A genome in T. turgidum and T. aestivum. This revelation therefore excludes T. urartu from the list of putative B-genome donors. To determine which of the remaining species is the source of the B chromosome set, the amino acid sequences of their purothionins were compared with that of the α1 purothionin coded for by the Pur-1B gene on chromosome 1 in the B genome of T. turgidum and T. aestivum. The residue sequences of this protein from Ae. bicornis, Ae. longissima, Ae. searsii, Ae. sharonensis, and Ae. speltoides differed by 1, 6, 1, 1, and 2 amino acid substitutions, respectively, from the α1 protein. These results suggest that either Ae. bicornis, Ae. searsii, or Ae. sharonensis was the most likely donor of the B genome. If the B genome in the polyploid wheats is monophyletic in origin, the collective findings of this and other investigations indicate that Ae. searsii is the most likely donor. The possibility that the B genome in the polyploid wheats could have a polyphyletic origin is also discussed.Key words: polyploid wheats, putative B-genome donors, purothionins, monophyletic, polyphyletic.

Characterization of genomes of timothy (Phleum pratense L.). I. Karyotypes and C-banding patterns in cultivated timothy and two wild relatives

Genome ◽  
1991 ◽  
Vol 34 (1) ◽  
pp. 52-58 ◽  
Author(s):  
Q. Cai ◽  
M. R. Bullen
Keyword(s):  
Phleum Pratense ◽  
Wild Relatives ◽  
Banding Patterns ◽  
C Banding ◽  
B Genome ◽  
A Genome ◽  
Cultivated Species ◽  
Genome Donors ◽  
Phleum Pratense L

In an attempt to know the phylogeny of timothy (Phleum pratense), the cultivated species and two wild relatives, Phleum alpinum and Phleum bertolonii, were karyotyped with conventional and Giemsa C-banding methods. In the hexaploid P. pratense (2n = 6x = 42), two sets of seven chromosomes were indistinguishable from each other both in morphology and in banding patterns and the third set of seven was found to be differentiated from them. Two genomes, A and B, were tentatively established. The banded karyotype in diploid P. alpinum (2n = 2x = 14) was close to the A genome, which was tetraploid in P. pratense, and the karyotype in P. bertolonii (2n = 2x = 14) was analogous to the B genome in P. pratense, which suggests these species were the genome donors of P. pratense.Key words: chromosome, genome, allopolyploid, Giemsa C-banding.

scholarly journals Genetic divergence study in salinity stress tolerant maize (Zea mays L.)

2015 ◽  
Vol 39 (4) ◽  
pp. 621-630 ◽  
Author(s):  
A Biswas ◽  
U Sarker ◽  
BR Banik ◽  
MM Rohman ◽  
MA Khaleque Mian
Keyword(s):  
Salinity Stress ◽  
Stress Condition ◽  
Zea Mays L ◽  
Principal Component ◽  
Inbred Lines ◽  
Spad Value ◽  
Significant Difference ◽  
Cluster Distance ◽  
Maize Inbreds ◽  
100 Seed Weight

The study was conducted to investigate the genetic diversity of some maize inbreds under salinity stress condition using Mahalanobis’s statistic (D2) and principal component analysis. Analysis of variance showed significant difference for all the characters. Results of multivariate analysis revealed that seventeen inbred lines formed five clusters at 12 dS level of salinity. The highest intra-cluster distance was recorded in cluster IV containing three genotypes and the lowest was in cluster V having one genotype. The inter cluster D2 values revealed maximum distance among the clusters. The highest inter cluster distance was observed between clusters IV & III and lowest was between V & I. Cluster IV had the highest cluster means for cob height, tassel length, cob length, SPAD value, number of seeds/cob, 100 seed weight, cob diameter and grain yield per plant. Considering cluster distance, inter-genotypic distance, cluster mean and other agronomic performances the genotypes CZ29, CZ33 and P43 from cluster IV and E135, E158, E169, P29 and P45 from cluster III may be considered as better parents for future hybridization programs to obtain desirable segregates in respect of different yield and yield contributing characters under salinity stress. DOI: http://dx.doi.org/10.3329/bjar.v39i4.22540 Bangladesh J. Agril. Res. 39(4): 621-630, December 2014

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