Use of dry matter content as a rapid and low-cost estimate for ranking genotypic differences in water-soluble carbohydrate concentrations in the stem and leaf sheath of Triticum aestivum

2009 ◽  
Vol 60 (1) ◽  
pp. 51 ◽  
Author(s):  
Gang-Ping Xue ◽  
C. Lynne McIntyre ◽  
Allan R. Rattey ◽  
Anthony F. van Herwaarden ◽  
Ray Shorter

Stem water-soluble carbohydrates (WSC) are an important source of temporary carbohydrate reserve in cool-season cereals. Genotypic variation in stem WSC concentration in wheat at anthesis is often positively associated with grain weight and yield in water-limited environments. In this study we have examined the relationship between dry matter content (DMC, dry weight per unit of fresh weight) and WSC concentration in field-grown bread wheat. Strong correlations (r = 0.92–0.95) were observed between DMC and WSC concentration in the stem and leaf sheath from the top two or three internodes of recombinant inbred lines from a cross between Seri M82 and Babax, at anthesis or 1 week after anthesis, in several field experiments. This strong correlation was also observed in diverse genotypes grown under rainfed or irrigated conditions. DMC and WSC concentration were also positively correlated in the whole above-ground biomass of wheat at anthesis (r = 0.74–0.91). Measurement of stem and leaf sheath DMC and WSC concentration in a small number of samples would allow the rapid prediction of WSC concentrations in a large number of field samples with reasonable accuracy, as demonstrated in a small dataset in this study. These data indicate that DMC can serve cereal breeding as a rapid and low-cost selection tool for genotypic ranking of WSC concentrations in breeding populations.

1989 ◽  
Vol 113 (1) ◽  
pp. 109-114 ◽  
Author(s):  
P. I. Orphanos ◽  
C. Metochis

SUMMARYTopping of tobacco (Nicotiana tabacum L.) plants (removal of the inflorescence) is not practised in Cyprus. Two field experiments were carried out at Akhelia Station, in 1985 and 1986, to test whether topping increased the yield or improved leaf quality of flue-cured tobacco grown under different types of irrigation. In contrast to the results of other studies, topping when the corolla had appeared on half the flowers did not increase yield. Topping when the inflorescence was just clear of the leaves (21 leaves harvested from topped plants v. 32 leaves from untopped plants) increased fresh yield by 7·0% but dry matter yield by 18·0%. The effects of topping were more marked on the leaves reaped at the third and fourth harvests, which constituted 52% of the fresh yield or 60% of the dry matter yield. At these harvests, leaves from topped plants had a lower percentage of midrib material (18·5 v. 21·0%) than leaves from untopped plants but a higher dry matter content (19·2 v. 17·2%) and specific leaf weight (80·0 v. 56·0 g/m2). Leaves of the fourth harvest from topped plants (27% of the total fresh yield) contained appreciably more nicotine (1·34%) than leaves from untopped plants (0·40%) but had a similar content of reducing sugars (20%). Topping did not influence the N, P and K contents of the leaves.Irrigation increased fresh yield from 12·0 t/ha (225 mm water) to 24·0 t/ha (450 mm water) and dry matter yield from 2·0 to 3·8 t/ha, respectively, in Expt 1.As Cypriot tobacco growers are paid for fresh yield delivered to the curing plant, the marginal increase of fresh yield with topping will hardly cover the cost of the extra operations involved.


2021 ◽  
Vol 901 (1) ◽  
pp. 012036
Author(s):  
A Bogdanova ◽  
A Payuta ◽  
A Alekseev ◽  
A Konovalov

Abstract The study is aimed at studying the effect of a microbiological preparation consisting of lactic acid bacteria, thermophilic streptococci and cellulolytic bacteria on the quality of alfalfa silage with different dry matter content. Determined pH, content of organic acids, dry matter and nutrients, including carbohydrates. Inoculant application increased crude protein, crude fat and crude fiber and reduced water-soluble carbohydrates in silage. The preservation of nutrients in the test samples was higher than that of the control. pH corresponded to optimal values; however, lactic acid fermentation proceeded more intensively in the sample with an increased dry matter content.


1984 ◽  
Vol 32 (2) ◽  
pp. 145-147
Author(s):  
P.C. Struik

Morphological and physiological characteristics of an ideal genotype are described. High dry-matter yield is required and therefore the ideotype needs to have high crop-density tolerance and efficient photosynthesis. Low susceptibility to pests and diseases is important. A stocky stem would benefit intake, yield and lodging resistance but reduce digestibility and dry-matter content. Selection for root-lodging resistance might reduce whole-plant yield. Improvement of cellular contents is of little importance, but cell-wall digestibility could be improved without strongly affecting yield. To be of high nutritional value, the stover must have sufficient dry-matter content (30-35%) and a moderate level of water-soluble carbohydrates. The ideotype should have an early silking date, a large ear and a slow rate of grain filling. (Abstract retrieved from CAB Abstracts by CABI’s permission)


2000 ◽  
Vol 51 (6) ◽  
pp. 749 ◽  
Author(s):  
T. A. Ciavarella ◽  
R. J. Simpson ◽  
H. Dove ◽  
B. J. Leury ◽  
I. M. Sims

The concentrations of water-soluble carbohydrate (WSC) and its components, starch, total nitrogen, and dry matter of phalaris (Phalaris aquatica L. cv. Australian) pasture were varied by shading for periods ranging from 38.5 to 46.5 h. In unshaded pasture, WSC concentrations were lowest at sunrise [103 mg/g dry matter (DM)] and increased until early afternoon (to 160 mg/g DM). Sucrose and starch increased in concentration during daylight, whilst the concentrations of glucose, fructose, fructan, and a component of WSC considered to be mainly the carbohydrate moiety of glycoside(s) were relatively constant. The concentrations of starch, and all components of WSC except sucrose, were reduced by shading, but increased to the concentrations observed in the unshaded pasture within 2–4 h after removal of the cover. The fructans present in phalaris were determined to be oligosaccharides of degree of polymerisation (DP) 3 and DP 4 and high molecular mass fructans with DP >10. Nitrogen concentration of shaded pasture was initially higher (4.7% DM) than in unshaded pasture (3.9% DM), but decreased after removal of the shade cover. Dry matter content was reduced in shaded pasture, partly due to increased retention of water on the exterior of plants. The experiment was a precursor for a grazing trial in which the WSC content of pasture was to be altered by shading. It indicated that shading would potentially alter WSC and N concentrations, and DM content, but would have only a relatively small impact on the digestibility of the pasture.


1993 ◽  
Vol 73 (2) ◽  
pp. 343-354 ◽  
Author(s):  
R. Beaulieu ◽  
J. R. Seoane ◽  
P. Savoie ◽  
D. Tremblay ◽  
R. Thériault ◽  
...  

Thirty-two sheep (30.0 kg avg. BW) were used to study the effects of dry-matter (DM) content at harvest on the nutritive value of timothy grass silages conserved as round bales of high (52.1%, DM50), medium (39.9%, DM40) and low (23.1%, DM25) DM. Chopped grass of 24.4% DM conserved in a horizontal silo (HS) was used as a control. Gross energy, crude protein (CP) and acid detergent fibre (ADF) contents were similar for all silages (P > 0.05). Neutral detergent fiber (NDF) contents decreased as DM of the silages decreased (P < 0.05). Acid detergent lignin and ash contents were highest in HS and lowest in DM40 silages (P < 0.05). Silage pH and water-soluble carbohydrates decreased with decreasing DM of the silages (P < 0.05), while ammonia-N and lactate levels increased (P < 0.05). Acetate concentrations in round bales were lower than in HS silage (P < 0.05). Significant butyrate concentrations were detected only in DM25 silage. Round-bale silages were chopped before feeding and fed ad libitum. DM intake was 14.5% higher for DM50 and DM40 silages than for DM25 and HS silages (P < 0.001). Average daily gain and feed efficiency were higher for high-DM silages than for DM25 and HS silages (P < 0.01). Apparent digestibilities of NDF, cellulose and crude fiber of the silages were similar, but DM, organic matter, energy and hemicellulose digestibilities were higher for HS than for DM25 silage. Apparent digestibilities of ADF and CP were higher for high-DM silages than for DM25 and HS silages (P < 0.04). The results indicate that to obtain good-quality silage, the DM content of round bales should be 40–50%. The better performance obtained with high-DM silages was indicative of a more efficient utilization of metabolizable energy for gain. Key words: Round-bale silage, timothy, forage


Agriculture ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 11 ◽  
Author(s):  
Thomas Hartinger ◽  
Nina Gresner ◽  
Karl-Heinz Südekum

Pre-ensiling treatments can significantly influence the composition of lucerne (Medicago sativa L.) silages (LS). Besides dry matter (DM) content and availability of water-soluble carbohydrates (WSC), wilting intensity may exert a strong impact on the crude protein (CP; nitrogen [N] × 6.25) fractions. The present study aimed to evaluate the effects of DM level, wilting intensity, and sucrose addition on N compounds and fermentation products in LS. Pure lucerne stand (cultivar Plato) was wilted with either high or low intensity to DM contents of 250 and 350 g kg−1, respectively, and ensiled with or without the addition of sucrose. Non-protein-N (NPN) concentration in LS was affected by all pre-ensiling treatments and with 699 g kg−1 CP, NPN was lowest in high-intensity wilted high-DM LS with sucrose addition. No effects were observed on in vitro-estimated concentrations of utilizable CP at the duodenum, a precursor to metabolizable protein. Sucrose addition and higher DM level decreased acetic acid and ammonia-N concentration in the silages. Therefore, the present study demonstrated the beneficial manipulation of CP fractions in LS by high-intensity wilting to higher DM contents and that the provision of WSC may be necessary for sufficient silage fermentation and protein preservation.


1988 ◽  
Vol 60 (2) ◽  
pp. 135-152
Author(s):  
Aino-Maija Evers

The effects of different fertilization practices on the carrot root and shoot dry weights, yield, dry matter contents and root/shoot ratio were studied in field experiments. Unirrigated and irrigated placement and broadcast fertilization, fertirrigations without basic fertilization, fertirrigations with basic fertilization, and PK placement with N fertirrigations were compared. In addition, split application was compared to single application of nutrients. The soil nutrient levels were studied by soil analysis. The carrot root and shoot samples were collected three times during the growing period. Cv. Nantes Duke Notabene 370 was grown. The carrot shoots reached their maximum weight already by three months, whereas roots grew slowly during the first two months but grew considerably during both the third and fourth month. The root and shoot dry weights were positively correlated at all sampling dates in both years ( r ≥0.77***), The big photosynthetizing apparatus caused the production of high root yield. The placement fertilization of granular NPK fertilizer increased the yield as compared to broadcast of granular NPK fertilizer (p = 0.009**). The yield was also increased by the placement of P and K as compared to treatments without placement fertilization of P and K (p = 0.03*). There was no difference in yields between single application and split application. Irrigation decreased the yield in 1985 (p = 0.03*). In 1986the yield was significantly higher than in 1985 (p = 0.000***). At harvest the fertilization did not affect the dry matter content of carrot roots when compared with unfertilized treatments. The difference in dry matter content was greater between years (p = 0.000***) than between fertilization practices (p = 0.05*). The treatment PK placement with N fertirrigations yielded carrots with a higher dry matter content than did the NPK fertirrigations treatments. Besides the fertilization experiment samples from two organically cultivated fields were collected to obtain data concerning organically cultivated carrots and soils.


2021 ◽  
Author(s):  
Jenna Hershberger ◽  
Edwige Gaby Nkouaya Mbanjo ◽  
Prasad Peteti ◽  
Andrew Smith Ikpan ◽  
Kayode Ogunpaimo ◽  
...  

Over 800 million people across the tropics rely on cassava as a major source of calories. While the root dry matter content (RDMC) of this starchy root crop is important for both producers and consumers, characterization of RDMC by traditional methods is time-consuming and laborious for breeding programs. Alternate phenotyping methods have been proposed but lack the accuracy, cost, or speed ultimately needed for cassava breeding programs. For this reason, we investigated the use of a low-cost, handheld NIR spectrometer for field-based RDMC prediction in cassava. Oven-dried measurements of RDMC were paired with 21,044 scans of roots of 376 diverse clones from 10 field trials in Nigeria and grouped into training and test sets based on cross-validation schemes relevant to plant breeding programs. Mean partial least squares regression model performance ranged from R2p = 0.62 - 0.89 for within-trial predictions, which is within the range achieved with laboratory-grade spectrometers in previous studies. Relative to other factors, model performance was highly impacted by the inclusion of samples from the same environment in both the training and test sets. Random forest variable importance analysis of root spectra revealed increased importance in a region previously identified as predictive of water content in plants (~950 - 990 nm). With appropriate model calibration, the tested spectrometer will allow for field-based collection of spectral data with a smartphone for accurate RDMC prediction and potentially other quality traits, a step that could be easily integrated into existing harvesting workflows of cassava breeding programs.


1968 ◽  
Vol 71 (3) ◽  
pp. 393-403 ◽  
Author(s):  
A. J. Thomson ◽  
H. H. Rogers

SUMMARYResults from three trials are presented. The main variables were date of harvest, plant density and variety. Measurements were made of whole-crop yields and yields from plant fractions (leaf, stem, cob). Quality components—digestibility, acid-pepsin solubility, water-soluble carbohydrates, nitrogen and ash—were estimated for whole crop and fractions. There were successive samplings for quality components.Whole-crop yield from fractions and yield of quality components increased with density. Whole-crop yield differed significantly with harvesting date as did all fractions except cob, although the proportion of cob increased with time. The earlier harvesting date gave greater yields of quality components.There was no effect of density on the dry-matter content but later harvesting resulted in higher dry matter.The only quality component affected by density was nitrogen content but there were large effects of harvesting date on digestibility and the acid-pepsin components.Regressions were computed for quality components on dry-matter proportion of cob and the dry-matter content of the whole crop. Regressions were significant for the acid-pepsin soluble components and the water-soluble carbohydrate component on the proportion of cob. Regressions of digestibility and pepsin-soluble components (total and organic) on dry-matter content were significant.The implications of these findings to the breeding of maize varieties are discussed.


1978 ◽  
Vol 58 (4) ◽  
pp. 559-569 ◽  
Author(s):  
J. W. G. NICHOLSON ◽  
D. A. YOUNG ◽  
R. E. McQUEEN ◽  
H. DE JONG ◽  
F. A. WOOD

The potential use of green potato (Solanum tuberosum L.) vines as a livestock feed was investigated. Fresh vines contained less than 12% dry matter (DM) and did not produce a stable silage in plastic bag, laboratory silos. Satisfactory silages were produced by wilting or by the addition of barley, malt, barley + malt, molasses, chopped hay, chopped hay + barley + malt, formaldehyde and/or formic acid. Only the silages from wilted vines or those containing hay had a suitable consistency for storage and handling in conventional silage systems. Sheep readily ate a silage made from potato vines with the addition of 5% barley, 0.5% malted barley and 20% chopped hay or straw. The dry matter content of the vines from two cultivars changed only slightly over a 5-wk sampling period but the acid detergent fiber (ADF) content increased and the N content dropped. Except for the cultivar Wischip, there was little variation among nine cultivars sampled on the same day for pH (5.15–5.95), DM (10.3–12.9%) and ADF (26.5–32.4%). The N content ranged from 1.6 to 2.4% of the DM and the water soluble carbohydrate content from 7.5 to 3.3% of the DM. The levels of glycoalkaloids found should be safe for ruminant animals. It was concluded that potato vines harvested prior to senescence contain a useful level of nutrients for ruminants. Pesticide residues and mechanical systems for harvesting the vines are major concerns to be resolved before on-farm application can be recommended.


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