scholarly journals A Simple Isocratic HPLC Method for the Quantitation of 17 Cannabinoids

2021 ◽  
Author(s):  
Peter Galettis ◽  
Michelle Williams ◽  
Rebecca Gordon ◽  
Jennifer H. Martin
Keyword(s):  
Hplc Method ◽  
Isocratic Hplc

Fast and isocratic HPLC-method for steviol glycosides analysis from Stevia rebaudiana leaves

2012 ◽  
Vol 7 (2) ◽  
pp. 147-154 ◽  
Author(s):  
Dominik Bergs ◽  
Bernhard Burghoff ◽  
Matthias Joehnck ◽  
Georg Martin ◽  
Gerhard Schembecker
Keyword(s):  
Stevia Rebaudiana ◽  
Hplc Method ◽  
Steviol Glycosides ◽  
Isocratic Hplc

Simultaneous determination of four vasoactive phytochemicals in different pharmaceutical preparations by a simple HPLC-DAD method

2016 ◽  
Vol 8 (8) ◽  
pp. 1858-1866 ◽  
Author(s):  
Fawzia Ibrahim ◽  
Mohie Khaled Sharaf El-Din ◽  
Rania Nabih El-Shaheny ◽  
Asmaa Kamal El-Deen ◽  
Kuniyoshi Shimizu
Keyword(s):  
Ascorbic Acid ◽  
Simultaneous Determination ◽  
Pharmaceutical Preparations ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Isocratic Hplc

A new, simple isocratic HPLC method was developed and validated for the simultaneous estimation of four vasoactive phytochemicals: ascorbic acid (ASC), rutin (RUT), hesperidin (HSP), and diosmin (DSM) in different pharmaceutical preparations.

scholarly journals Application and Validation of Simple Isocratic HPLC-UV-DAD Method with Dual Wavelength Detection for Ivabradine Determination and Its Application in the Study of Stress Degradation

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Joanna Nowakowska ◽  
Piotr Pikul ◽  
Marcin Marszałł ◽  
Krzesimir Ciura
Keyword(s):  
Degradation Products ◽  
Hplc Method ◽  
Simple Method ◽  
Degradation Study ◽  
Modern Drug ◽  
Stress Degradation ◽  
Isocratic Hplc ◽  
Cardiac Energy ◽  
Wavelength Detection

Ivabradine is a modern drug that selectively lowers the heart rate, improves cardiac energy balance, and reduces heart’s demands for oxygen and energy. Due to the chemical nature of ivabradine, which absorbs light at 207 nm and 286 nm, its detection was performed at two wavelengths. A Knauer C8 column was used to develop the RP-HPLC method for determination of ivabradine. The proposed method was linear from 5 to 100 µg/ml (r>0.999) for both wavelengths and limits of detection (LOD) and limits of quantification (LOQ) were 0.33 and 1.09 µg/ml for 207 nm and 1.19 and 3.97 µg/ml for 286 nm, respectively. After validation, the investigated method was applied to a stress degradation study. Numerous degradation products were formed from ivabradine solutions through alkaline and acid hydrolysis, oxidation, and photolysis. The largest numbers of degradation products were found in the sample exposed to 24 h radiation and alkaline hydrolysis (eight and six products, resp.). Finally, the simple method using HPLC-UV-DAD was developed and validated. Its usefulness for the monitoring of possible degradation products was demonstrated.

scholarly journals Validation of an isocratic HPLC method to detect 2-fluoro-β-alanine for the analysis of dihydropyrimidine dehydrogenase activity

2010 ◽  
Vol 878 (21) ◽  
pp. 1889-1892
Author(s):  
Kinta M. Serve ◽  
Jennifer L. Darnell ◽  
Jody K. Takemoto ◽  
Neal M. Davies ◽  
Margaret E. Black
Keyword(s):  
Dehydrogenase Activity ◽  
Dihydropyrimidine Dehydrogenase ◽  
Hplc Method ◽  
Isocratic Hplc

scholarly journals A Simple and Effective Isocratic HPLC Method for Fast Identification and Quantification of Surfactin

2015 ◽  
Vol 44 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Muhammad Qadri Effendy Mubarak ◽  
Abdul Rahman Hassan ◽  
Aidil Abdul Hamid ◽  
Sahaid Khalil ◽  
Mohd. Hafez Mohd. Isa
Keyword(s):  
Hplc Method ◽  
Fast Identification ◽  
Isocratic Hplc ◽  
Identification And Quantification

Validation of Single Isocratic HPLC Method for the Assay of Valdecoxib and Determination of Metaisomer Impurity

2009 ◽  
Vol 47 (4) ◽  
pp. 309-314
Author(s):  
K. Karthikeyan ◽  
R. Saravanan ◽  
R. Rajeswari ◽  
K. C. Pillai
Keyword(s):  
Hplc Method ◽  
Isocratic Hplc

A Simple Improved Method for the Measurement of Cyclosporin by Liquid-Liquid Extraction of Whole Blood and Isocratic HPLC

1988 ◽  
Vol 25 (5) ◽  
pp. 510-515 ◽  
Author(s):  
Stephen J Fletcher ◽  
Robby A Bacchus
Keyword(s):  
Whole Blood ◽  
Hplc Method ◽  
Improved Method ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Liquid Liquid Extraction ◽  
Routine Measurement ◽  
Ether Extraction ◽  
Acid Wash ◽  
Isocratic Hplc

The current HPLC methods of cyclosporin measurement have been reviewed and all aspects assessed. A simple isocratic C-18 reverse phase HPLC method with improved efficiency is described for the routine measurement of cyclosporin in whole blood. An alkaline ether extraction is followed by an acid wash, solvent evaporation and two hexane washes of the reconstituted extract. The turn-round time for a single sample is 1 h. Daily batches of up to 40 patient samples can be easily measured with this method. The results are compared with those from the Sandoz radioimmunoassay (RIA) method.

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