Simultaneous determination of four vasoactive phytochemicals in different pharmaceutical preparations by a simple HPLC-DAD method

2016 ◽  
Vol 8 (8) ◽  
pp. 1858-1866 ◽  
Author(s):  
Fawzia Ibrahim ◽  
Mohie Khaled Sharaf El-Din ◽  
Rania Nabih El-Shaheny ◽  
Asmaa Kamal El-Deen ◽  
Kuniyoshi Shimizu
Keyword(s):  
Ascorbic Acid ◽  
Simultaneous Determination ◽  
Pharmaceutical Preparations ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Isocratic Hplc

A new, simple isocratic HPLC method was developed and validated for the simultaneous estimation of four vasoactive phytochemicals: ascorbic acid (ASC), rutin (RUT), hesperidin (HSP), and diosmin (DSM) in different pharmaceutical preparations.

Simultaneous Determination of L-Ascorbic Acid and L-Ascorbylpalmitate Using RP-HPLC Method

2013 ◽  
Vol 716 ◽  
pp. 465-469
Author(s):  
Ye Lin Tian ◽  
Jun Kai Wang ◽  
Ping Sheng Leng ◽  
Yun Liu
Keyword(s):  
Ascorbic Acid ◽  
Simultaneous Determination ◽  
Methyl Palmitate ◽  
High Performance ◽  
Correlation Coefficients ◽  
Reversed Phase ◽  
Hplc Method ◽  
Enzymatic Transesterification ◽  
Rp Hplc

A rapid reversed phase highperformance liquid chromatography (RP-HPLC) method was developed for the simultaneous analysis of L-ascorbic acid and L-ascorbylpalmitate (AP). The chromatography was performed on a SSI model 2300-525 high performance liquid chromatographequipped with anAlltechApollo C18 column at 30 oC.The mobile phase was acetonitrile-water (90:10,v/v) with the flow rate of 1.0 mL/min. UV detection wavelength was 250 nm. This method permits the simultaneous determination of ascorbic acid and AP in the synthesis of AP transesterified with methyl palmitate and ascorbic acid. The detection limit of ascorbic acid and AP was 0.07μg/mL and 0.12μg/mL, respectively. The recovery was 90.59 ± 3.04% for ascorbic acid and 101.3 ± 4.81% for AP. The linearity range for ascorbic acid and AP was in the range of 0.1 - 0.7 mg/mL and 0.4 - 4.0 mg/mL, respectively. Correlation coefficients (R2) were 0.9910 for ascorbic acid and 0.9986 for AP. The proposed method could be used for routine quality control of AP synthesis with methyl palmitate and ascorbic acidby enzymatic transesterification.

scholarly journals Stability-indicating RP-HPLC method for simultaneous estimation of levosalbutamol sulfate and theophylline in combined dosage form

2013 ◽  
Vol 49 (3) ◽  
pp. 475-490 ◽  
Author(s):  
Sagar Suman Panda ◽  
Bera Venkata Varaha Ravi Kumar ◽  
Ganeswar Mohanta
Keyword(s):  
Simultaneous Determination ◽  
Dosage Form ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Rp Hplc ◽  
Photolytic Degradation ◽  
Tetrabutyl Ammonium ◽  
System Suitability ◽  
Ammonium Hydrogen

A novel, simple, accurate and precise RP-HPLC method for simultaneous determination of levosalbutamol sulfate and theophylline has been developed and validated. Separation was achieved on a Phenomenex; C18 column (250 mm × 4.6 mm i.d., 5 µm) using methanol: 10 mM TBAHS(tetrabutyl ammonium hydrogen sulfate) (50:50, v/v) as mobile phase at flow rate of 1.0 mL.min-1. The UV detection wavelength was 274 nm. The linearity is obeyed over a concentration range of 0.5-150 µg.mL-1 with correlation coefficient of 0.999 for both the drugs. The proposed method was validated by determining accuracy, precision, stability and system suitability parameters. The method was found to be robust. Specificity of the method was determined by subjecting the drugs to various stress conditions like acid, alkali, oxidation, thermal and photolytic degradation. The method was used successfully for the simultaneous determination of levosalbutamol sulfate and theophylline in syrup dosage form.

A new HPLC method for the simultaneous determination of ascorbic acid and aminothiols in human plasma and erythrocytes using electrochemical detection

Talanta ◽  
2011 ◽  
Vol 84 (3) ◽  
pp. 789-801 ◽  
Author(s):  
Abad Khan ◽  
Muhammad I. Khan ◽  
Zafar Iqbal ◽  
Yasar Shah ◽  
Lateef Ahmad ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Simultaneous Determination ◽  
Human Plasma ◽  
Electrochemical Detection ◽  
Hplc Method
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