Cationic Micelles Based on Polyhedral Oligomeric Silsesquioxanes for Enhanced Gene Transfection

2016 ◽  
Vol 69 (4) ◽  
pp. 363 ◽  
Author(s):  
Connie K. Liu ◽  
Qingqing Dou ◽  
Sing Shy Liow ◽  
Jatin N. Kumar ◽  
Xian Jun Loh
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
Polyhedral Oligomeric Silsesquioxane ◽  
Amphiphilic Copolymers ◽  
Polyhedral Oligomeric Silsesquioxanes ◽  
Ethyl Methacrylate ◽  
Cationic Micelles ◽  
A Cell ◽  
Oligomeric Silsesquioxane

The synthesis and gene transfection efficiency of a series of amphiphilic copolymers, poly(2-(dimethylamino)ethyl methacrylate)-poly (methacrylate isobutyl polyhedral oligomeric silsesquioxane) (PDMAEMA-POSS) copolymers are reported. The hydrophobic POSS interior allows a cell-sensitizing drug such as paclitaxel to be incorporated, whereas the cationic and hydrophilic PDMAEMA corona allows the complexation of anionic DNA to form a nano-sized polyplex. These drug-encapsulated copolymers display excellent gene transfection efficiency compared with polyethylenimine or PDMAEMA homopolymers.

Tuning “thiol-ene” reactions toward controlled symmetry breaking in polyhedral oligomeric silsesquioxanes

2014 ◽  
Vol 5 (3) ◽  
pp. 1046-1053 ◽  
Author(s):  
Yiwen Li ◽  
Kai Guo ◽  
Hao Su ◽  
Xiaopeng Li ◽  
Xueyan Feng ◽  
...  
Keyword(s):  
Symmetry Breaking ◽  
Polyhedral Oligomeric Silsesquioxane ◽  
Building Blocks ◽  
Polyhedral Oligomeric Silsesquioxanes ◽  
Ene Reactions ◽  
Oligomeric Silsesquioxane

A facile method toward polyhedral oligomeric silsesquioxane-based nano-building blocks with controlled symmetry breaking was reported by using thiol-ene chemistry.

Poly(styrene-b-butadiene-b-styrene)-Dye-Coupled Polyhedral Oligomeric Silsesquioxanes

2010 ◽  
Vol 123-125 ◽  
pp. 169-172
Author(s):  
Steven Spoljaric ◽  
Robert A. Shanks
Keyword(s):  
Mechanical Properties ◽  
Thermal Stability ◽  
Block Copolymer ◽  
Exponential Function ◽  
Polyhedral Oligomeric Silsesquioxane ◽  
Element Model ◽  
Polyhedral Oligomeric Silsesquioxanes ◽  
Creep And Recovery ◽  
Oligomeric Silsesquioxane ◽  
Colour Coordinates

Dye-coupled polyhedral oligomeric silsesquioxane (POSS) were prepared and the coloured POSS particles were ultrasonically solution dispersed in poly(styrene-b-butadiene-b-styrene) (SBS). POSS molecules contained either isobutyl or phenyl groups to provide selective compatibility with either the soft (butadiene) or hard (styrene) phase within the block copolymer. The composition and thermal stability were characterised using thermogravimetry. Colour coordinates were measured. Tensile mechanical properties, creep and recovery were determined. Creep was modeled using the 4-element model of Maxwell and Kelvin-Voigt, while recovery correlated with the stretched-exponential function of Kohlrausch, Williams and Watts.

Fluorinated polyhedral oligomeric silsesquioxanes

RSC Advances ◽  
2015 ◽  
Vol 5 (6) ◽  
pp. 4547-4553 ◽  
Author(s):  
Xiaobai Wang ◽  
Qun Ye ◽  
Jing Song ◽  
Ching Mui Cho ◽  
Chaobin He ◽  
...  
Keyword(s):  
Polyhedral Oligomeric Silsesquioxane ◽  
Polyhedral Oligomeric Silsesquioxanes ◽  
Hydrosilylation Reaction ◽  
Oligomeric Silsesquioxane

A series of fluorinated polyhedral oligomeric silsesquioxane (POSS) oils were readily prepared via hydrosilylation reaction.

Unexpected fluorescence from maleimide-containing polyhedral oligomeric silsesquioxanes: nanoparticle and sequence distribution analyses of polystyrene-based alternating copolymers

2016 ◽  
Vol 7 (1) ◽  
pp. 135-145 ◽  
Author(s):  
Mohamed Gamal Mohamed ◽  
Kuo-Chih Hsu ◽  
Jin-Long Hong ◽  
Shiao-Wei Kuo
Keyword(s):  
Polyhedral Oligomeric Silsesquioxane ◽  
Polyhedral Oligomeric Silsesquioxanes ◽  
Sequence Distribution ◽  
Alternating Copolymers ◽  
Oligomeric Silsesquioxane

Unusual fluorescent polyhedral oligomeric silsesquioxane (POSS)-containing polymers lacking any common fluorescent units because of the crystallinity and clustering of locked CO groups of POSS units.

Polyhedral Oligomeric Silsesquioxanes (Poss): Silicon Based Monomers and their Use in the Preparation of Hybrid Polyurethanes

1998 ◽  
Vol 519 ◽  
Author(s):  
Joseph J. Schwab ◽  
Joseph D Lichtenhan ◽  
Kevin P. Chaffee ◽  
Patrick T Mather ◽  
Angel Romo-Uribe
Keyword(s):  
Polyhedral Oligomeric Silsesquioxane ◽  
Polyhedral Oligomeric Silsesquioxanes ◽  
Rheological Data ◽  
Chain Extenders ◽  
Step Growth ◽  
Silicon Based ◽  
Oligomeric Silsesquioxane

AbstractA series of Polyhedral Oligomeric Silsesquioxane (POSS) monomers bearing reactive hydroxyl functionalities, suitable for incorporation into step-growth polymers, is described. These monomers are difunctional in nature and are particularly well suited for use as chain extenders in the synthesis of polyurethanes. This work describes the synthesis of these POSS monomers and their incorporation into a series of polyurethanes. Preliminary thermal, mechanical and rheological data for the POSS containing polyurethanes will also be discussed.

scholarly journals Effect of Alkyl Chain Length in POSS Nanocage on Non-Isothermal Crystallization Behavior of PCL/Amino-POSS Nanocomposites

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1719 ◽  
Author(s):  
Fernández ◽  
Guzmán ◽  
Ramos ◽  
Fernández
Keyword(s):  
Size Distribution ◽  
Crystallization Temperature ◽  
Isothermal Crystallization ◽  
Crystallization Behavior ◽  
Crystallization Process ◽  
Polyhedral Oligomeric Silsesquioxane ◽  
Degree Of Crystallinity ◽  
Polyhedral Oligomeric Silsesquioxanes ◽  
Scanning Calorimetry ◽  
Oligomeric Silsesquioxane

The study of the non-isothermal crystallization behavior of polymers is of great importance due to the effect of degree of crystallinity and crystallization process on the polymer properties. The effect of aminopropylisobutyl polyhedral oligomeric silsesquioxane (APIBPOSS) and aminopropylisooctyl polyhedral oligomeric silsesquioxane (APIOPOSS) on poly(ε-caprolactone) (PCL) crystallization is studied by differential scanning calorimetry (DSC) under non-isothermal conditions and polarized optical microscopy (POM). The crystallization kinetics is analyzed using the Avrami and Mo models, and effective activation energies are evaluated by the Friedman isoconversional method. The results show that the compatibility between polyhedral oligomeric silsesquioxanes (POSS) and PCL and POSS loading affect the crystallization process. A higher crystallization temperature, a narrower size distribution of crystallite, and a faster crystallization rate are obtained in the presence of all the studied contents of APIBPOSS and at lower contents of APIOPOSS. At APIOPOSS contents higher than 2 wt %, the crystallization temperature is lowered, the size distribution of crystallite is broadened, and the crystallization process is retarded. The presence of POSS leads to an increase in the number of nucleation sites, and a reduction in the size of the crystallite and the overall degree of crystallinity, as a result of the confinement of PCL chains caused by POSS nanoparticles.

scholarly journals Lignin-Based Nonviral Gene Carriers Functionalized by Poly[2-(Dimethylamino)ethyl Methacrylate]: Effect of Grafting Degree and Cationic Chain Length on Transfection Efficiency

Biomolecules ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 102
Author(s):  
Xiaohong Liu ◽  
Hui Yin ◽  
Xia Song ◽  
Zhongxing Zhang ◽  
Jun Li
Keyword(s):  
Gene Delivery ◽  
Chain Length ◽  
Cell Lines ◽  
Transfection Efficiency ◽  
Graft Copolymers ◽  
Gene Transfection ◽  
Structural Factors ◽  
Ethyl Methacrylate ◽  
Branched Structure ◽  
Grafting Degree

Lignin is a natural renewable biomass resource with great potential for applications, while its development into high value-added molecules or materials is rare. The development of biomass lignin as potential nonviral gene delivery carriers was initiated by our group through the “grafting-from” approach. Firstly, the lignin was modified into macroinitiator using 2-bromoisobutyryl bromide. Then cationic polymer chains of poly[2-(dimethylamino)ethyl methacrylate] (PDMAEMA) were grown from the lignin backbone using atom transfer radical polymerization (ATRP) to yield lignin-PDMAEMA graft copolymers (LPs) with branched structure. To gain a deep understanding of the relationship between the nonviral gene transfection efficiency of such copolymers and their structural and compositional factors, herein eight lignin-based macroinitiators with different modification degrees (MDs, from 3.0 to 100%) were synthesized. Initiated by them, a series of 20 LPs were synthesized with varied structural factors such as grafting degree (GD, which is equal to MD, determining the cationic chain number per lignin macromolecule), cationic chain length (represented by number of repeating DMAEMA units per grafted arm or degree of polymerization, DP) as well as the content of N element (N%) which is due to the grafted PDMAEMA chains and proportional to molecular weight of the LPs. The in vitro gene transfection capability of these graft copolymers was evaluated by luciferase assay in HeLa, COS7 and MDA-MB-231cell lines. Generally, the copolymers LP-12 (N% = 7.28, MD = 36.7%, DP = 13.6) and LP-14 (N% = 6.05, MD = 44.4%, DP = 5.5) showed good gene transfection capabilities in the cell lines tested. Overall, the performance of LP-12 was the best among all the LPs in the three cell lines at the N/P ratios from 10 to 30, which was usually several times higher than PEI standard. However, in MDA-MB-231 at N/P ratio of 30, LP-14 showed the best gene transfection performance among all the LPs. Its gene transfection efficiency was ca. 11 times higher than PEI standard at this N/P ratio. This work demonstrated that, although the content of N element (N%) which is due to the grafted PDMAEMA chains primarily determines the gene transfection efficiency of the LPs, it is not the only factor in explaining the performance of such copolymers with the branched structure. Structural factors of these copolymers such as grafting degree and cationic chain length could have a profound effect on the copolymer performance on gene transfection efficiency. Through carefully adjusting these factors, the gene transfection efficiency of the LPs could be modulated and optimized for different cell lines, which could make this new type of biomass-based biomaterial an attractive choice for various gene delivery applications.

Synthesis, characterization and chondrocyte culture of polyhedral oligomeric silsesquioxane (POSS)-containing hybrid hydrogels

RSC Advances ◽  
2016 ◽  
Vol 6 (28) ◽  
pp. 23471-23478 ◽  
Author(s):  
Xiuwei Pan ◽  
Hao Gao ◽  
Guodong Fu ◽  
Yun Gao ◽  
Weian Zhang
Keyword(s):  
Ethylene Glycol ◽  
Click Reaction ◽  
Polyhedral Oligomeric Silsesquioxane ◽  
Polyhedral Oligomeric Silsesquioxanes ◽  
Poly Ethylene Glycol ◽  
Chondrocyte Culture ◽  
Hybrid Hydrogels ◽  
Poly Ethylene ◽  
Oligomeric Silsesquioxane

Polyhedral oligomeric silsesquioxanes (POSS)-based hybrid hydrogels were successfully prepared via a fast azide-alkyne click reaction between octa-azido-functionalized POSS (OAPOSS) and alkyne-functionalized poly(ethylene glycol) (PEG).

scholarly journals Reactive bay functionalized perylene monoimide-polyhedral oligomeric silsesquioxane organic electronic dye

2015 ◽  
Vol 33 (1) ◽  
pp. 113-121 ◽  
Author(s):  
Lodrick Makokha Wangatia ◽  
Bin Sun ◽  
Ting Zeng ◽  
Meifang Zhu
Keyword(s):  
Solid State ◽  
Photophysical Properties ◽  
Polyhedral Oligomeric Silsesquioxane ◽  
Perylene Diimide ◽  
Spherical Particles ◽  
High Tech ◽  
Polyhedral Oligomeric Silsesquioxanes ◽  
Bay Area ◽  
Solid Fluorescence ◽  
Oligomeric Silsesquioxane

AbstractAggregation-induced quenching is particularly detrimental in perylene diimides, which are characterized by a near-unity fluorescence quantum yield in solution but are far less emissive in the solid state. Previously, perylene diimide has been improved by linking it to the inorganic cage of polyhedral oligomeric silsesquioxanes. As a further study on perylene diimidepolyhedral oligomeric silsesquioxanes, we report on a double functionalized molecular structure, which can be used for substitution at the bay area and as a side group in other materials. Typical solution absorption and emission features of the perylene diimide fragment have been observed in this new reactive perylene diimide-polyhedral oligomeric silsesquioxane. Moreover, reduced stacking during aggregation and spherical particles exhibiting solid fluorescence have been obtained. Organic semiconducting material with enhanced solid state photophysical properties, like solid fluorescence is a subject of great interest owing to its possible high-tech applications in optoelectronic devices.

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