scholarly journals Histological investigation on Ancyrocephalus paradoxus (Dactylogyridea: Ancyrocephalidae) infection causing mortalities in an intensively cultured pikeperch [Sander lucioperca (L.)] stock

2016 ◽  
Vol 64 (2) ◽  
pp. 201-212
Author(s):  
Kálmán Molnár ◽  
Gábor Szilágyi ◽  
Gábor Mosonyi ◽  
Ádám Varga ◽  
Csaba Székely
Keyword(s):  
Epithelial Cells ◽  
Connective Tissue ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Histological Investigation ◽  
Pathological Changes ◽  
Sander Lucioperca ◽  
Gill Parasite ◽  
Attachment Sites ◽  
Gill Filaments

In a cultured pikeperch (Sander lucioperca) stock the monopisthocotylean monogenean gill parasite Ancyrocephalus paradoxus caused heavy infection and mortalities. The gills of the affected fish specimens were infected by 50 to 800 monogenean parasites. Severe pathological changes were found in areas where the worms attached to the gills. At the attachment sites the haptoral discs of the worms formed a deep depression in the epithelium of the filaments, and the anchors pierced into and fixed themselves to the connective tissue of the cartilaginous gill rays. At these attachment sites red blood cells released from injured capillaries were found among the damaged epithelial cells. Around the hooks, anchors and body sections coming into contact with the gill filaments a proliferative tissue developed in which only a remnant of the damaged lamellae was found. Due to the damage caused by the worms the tips of the heavily infected gill filaments fused, formed clubs and were composed of epitheloid-type regeneration tissue lacking respiratory lamellae. In the basal parts of the filaments, where most of the worms attached to the gill, only denuded filaments deprived of lamellae were observed among the cross-sectioned worms in histological sections.

Chloride and taurine effluxes occur by different pathways in skate erythrocytes

1996 ◽  
Vol 271 (6) ◽  
pp. R1544-R1549 ◽  
Author(s):  
E. M. Davis-Amaral ◽  
M. W. Musch ◽  
L. Goldstein
Keyword(s):  
Epithelial Cells ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Disulfonic Acid ◽  
Raja Erinacea ◽  
Western Blots ◽  
Cl Channel ◽  
Cl Channels

The aim of this study was to determine whether volume-activated taurine and Cl- effluxes occur via the same system in skate (Raja erinacea) red blood cells (RBC). The effluxes were measured in isotonic and hypotonic elasmobranch Ringer solutions, in which NaCl was replaced by mannitol and the remaining exchangeable anions with gluconate. Methazolamide (0.1 mM) was added to minimize HCO3- formation. RBC Cl- content fell approximately 50%/h in both isotonic and hypotonic media, with no detectable K- loss in either medium. The observed Cl- loss was accompanied by an increase in pH. Both the Cl- loss and pH rise were inhibited by 4,4'- diisothiocyanostilbene-2,2'-disulfonic acid (0.1 mM), suggesting that Cl- efflux was due to H(+)-Cl- cotransport. 36Cl- effluxes in isotonic and hypotonic media were (means +/- SE, n = 11) 2.8 +/- 0.6 and 3.5 +/- 0.9 mumol.g dry wt RBC-1.min-1, respectively, whereas [3H]taurine effluxes in the same media were 0.045 +/- 0.02 and 2.1 +/- 0.05 mumol.g dry wt RBC-1.min-1, respectively (n = 6). These results indicate that taurine and Cl- effluxes occur via different pathways in skate RBC. In addition, the swelling-activated Cl- channel reported in epithelial cells does not appear to be present in skate RBC. This conclusion was confirmed by Western blots with an antibody to swelling-activated Cl- channels. Taurine and Cl- fluxes are apparently under different pathway influences in these RBC: taurine diffuses via a channel, whereas Cl- is transported by cotransporters.

scholarly journals Pneumatic tube transportation of urine samples

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Eline Sandvig Andersen ◽  
Ivan Brandslund
Keyword(s):  
Epithelial Cells ◽  
Red Blood Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
Urine Samples ◽  
Tube System ◽  
Pneumatic Tube ◽  
Local Quality ◽  
Renal Tubular ◽  
Pneumatic Tube System

AbstractObjectivesPneumatic tube transportation of samples is an effective way of reducing turn-around-time, but evidence of the effect of pneumatic tube transportation on urine samples is lacking. We thus wished to investigate the effect of pneumatic tube transportation on various components in urine, in order to determine if pneumatic tube transportation of these samples is feasible.MethodsOne-hundred fresh urine samples were collected in outpatient clinics and partitioned with one partition being carried by courier to the laboratory, while the other was sent by pneumatic tube system (Tempus600). Both partitions were then analysed for soluble components and particles, and the resulting mean difference and limits of agreement were calculated.ResultsAlbumin, urea nitrogen, creatinine, protein and squamous epithelial cells were unaffected by transportation in the Tempus600 system, while bacteria, renal tubular epithelial cells, white blood cells and red blood cells were affected and potassium and sodium may have been affected.ConclusionsThough pneumatic tube transportation did affect some of the investigated components, in most cases the changes induced were clinically acceptable, and hence samples could be safely transported by the Tempus600 pneumatic tube system. For bacteria, white blood cells and red blood cells local quality demands will determine if pneumatic tube transportation is appropriate.

scholarly journals Inhibition of Growth of Hair Follicles by a Lectin-like Substance from Rat Skin

1983 ◽  
Vol 36 (4) ◽  
pp. 411 ◽  
Author(s):  
R Frater
Keyword(s):  
Molecular Weight ◽  
Epithelial Cells ◽  
Dna Synthesis ◽  
Affinity Chromatography ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Hair Follicles ◽  
Rat Skin ◽  
Small Molecular Weight ◽  
Inhibition Of Growth

A small molecular weight (5000--10000) substance has been isolated from rat skin by affinity chromatography on a column of acid-hydrolysed Sepharose. The substance agglutinates rabbit red blood cells, inhibits DNA synthesis in rat hair follicles, and causes the appearance of autophagic vacuoles in the epithelial cells of the lower follicle bulb.

scholarly journals CHANGES IN THE MORPHOLOGIC STATE OF RED BLOOD CELLS IN PATIENTS WITH POLYTRAUMA

2020 ◽  
Vol 4 ◽  
pp. 64-70
Author(s):  
Mariia Matvieienko ◽  
Alena Gryshchenko ◽  
Nadiia Baranova ◽  
Arora Sukesh ◽  
Ievgen Bausov
Keyword(s):  
Red Blood Cells ◽  
Multiple Trauma ◽  
Blood Cells ◽  
Early Period ◽  
Recovery Process ◽  
Pathological Changes ◽  
Rbc Indices ◽  
Red Blood Cell Indices ◽  
Positive Effect

The measurement of RBC indices is of particular importance as an indirect intermediate component of the recovery process after exposure to hypoxia, ischemia, hypothermia in patients with traumatic disease due to polytrauma. The aim of this study was to evaluate the parameters of the morphologic state of red blood cells as a reaction of patients with traumatic disease. Materials and methods.A prospective study was carried out evaluation of 120 patients suffering from polytrauma. The patients were divided into 3 groups according to the principle of using additional substances as a part of IC. The parameters of morphologic state of red blood cells were studied. Results and discussion. The course of acute and early periods of traumatic disease has accompanied variability morphological forms of red blood cells, as evidenced by a decrease in MCV and RDW increased levels of the 3rd to 5th day. The optimized therapy used in the treatment of patients, has a positive effect on the parameters of red blood cells, contributes to a better recovery of red blood cells after hypoxia resulting multiple trauma. Conclusion. The study has demonstrated variations in the morphologic properties of red blood cells in the acute and early period of traumatic disease, as confirmed by pathological changes of the erythrocyte indices. The administration of the proposed therapy with the use of D-fructose-1.6-diphosphate sodium salt of hydrate has a positive effect on the parameters of red blood cells, contributes to a better recovery after hypoxia resulting multiple trauma. Given this, the calculations of the red blood cell indices may carry additional diagnostic information, which allows identify trends for not obvious pathological changes and quality of treatment.

scholarly journals The Presence of Circulating Nucleated Red Blood Cells Is Associated With Disease Severity in Patients of Hemorrhagic Fever With Renal Syndrome

2021 ◽  
Vol 8 ◽  
Author(s):  
Jingang Zhang ◽  
Kang Tang ◽  
Yun Zhang ◽  
Ying Ma ◽  
Chunmei Zhang ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Disease Severity ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Expression Profiles ◽  
Hemorrhagic Fever ◽  
Hantaan Virus ◽  
Pathological Changes ◽  
Hematological Indices ◽  
Nucleated Red Blood Cells

Hemorrhagic fever with renal syndrome (HFRS) is a regional infectious disease of epidemic potential caused by the Hantaan virus (HTNV). Red blood cells (RBCs) are the major components of peripheral blood. However, pathological changes in RBCs and the underlying mechanisms during HTNV infection remain largely unclear. Therefore, this study sought to explore changes in RBCs in the peripheral blood of HFRS patients. We isolated PBMCs from HFRS patients and performed single-cell RNA sequencing. The results showed that clusters of RBCs in the peripheral blood of HFRS could be classified as nucleated red blood cells (NRBC) based on their cellular components, gene expression profiles and cell surface markers. In addition, it was shown that the higher the count of NRBC in peripheral blood, the more severe the disease status was. Moreover, hematological indices related to RBCs were analyzed and the results showed that impairment in the folate pathway might be the possible reason behind the presence of NRBCs. This study, for the first time showed that the presence of NRBCs in the peripheral blood of HFRS patients was associated with disease severity. This was also the first study to show that infection with the HTNV virus hindered the maturation of RBCs. Therefore, this work provides further insights on the role of and pathological changes in RBCs during HTNV infection.

scholarly journals УЛЬТРАМІКРОСКОПІЧНІ ПРОЯВИ У СТРУКТУРІ ПІДНИЖНЬОЩЕЛЕПНОЇ СЛИННОЇ ЗАЛОЗИ НАПРИКІНЦІ 2-ГО ТИЖНЯ ЕКСПЕРИМЕНТАЛЬНОГО ОПІОЇДНОГО ВПЛИВУ

World Science ◽  
2020 ◽  
Vol 1 (4(56)) ◽  
pp. 4-7
Author(s):  
Mykhalevych Marta
Keyword(s):  
Endothelial Cells ◽  
Endoplasmic Reticulum ◽  
Salivary Gland ◽  
Connective Tissue ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Acinar Cells ◽  
Ultrastructural Changes ◽  
Submandibular Salivary Gland ◽  
Perivascular Edema

This publication demonstrates the ultrastructural changes of the submandibular salivary gland under the opioid effect at the 2nd week of the experimental research.After 14 days of experimental opioid effect, we found the destructive changes in the organelles of exocrinocytes of acinar cells, as well as dyscirculatory processes. Capillary lumen was enlarged, overflowing with red blood cells. In the endothelium of the capillaries also noted the expansion of the granular endoplasmic reticulum. Small vacuoles appeared in the cytoplasm of endothelial cells. Single mitochondria located in the area of the endothelial cytoplasmic organelles are swollen. Due to the development of perivascular edema, the underlying connective tissue substance around the capillaries was impregnated with electron-illuminated transudate masses.

DIAGNOSTIC CAPABILITIES OF THE ELECTROPHORETIC MOBILITY OF RED BLOOD CELLS AND BUCCAL CELLS IN STRESS

2019 ◽  
pp. 106-111
Author(s):  
А.В. Дерюгина ◽  
М.Н. Иващенко ◽  
П.С. Игнатьев ◽  
А.Г. Самоделкин ◽  
М.В. Золотова ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Red Blood Cells ◽  
Electrophoretic Mobility ◽  
Blood Cells ◽  
Information Technologies ◽  
Stress Reaction ◽  
Medical Laboratory ◽  
Sharp Change ◽  
Epinephrine Injection ◽  
Acting Force

Диагностика стресс-реактивности по анализу гормонального состояния организма, является сложной, экономически затратной и трудоемкой процедурой исследования. В связи с этим актуален поиск информационных технологий, которые достаточно легко реализуются в любой медицинской лаборатории и позволяют оценить стресс-реакцию организма. Цель работы - оценка диагностических возможностей электрофоретической подвижности эритроцитов и клеток буккального эпителия в процессе развития стрессовой реакции. Методика. Для моделирования стресса использовали внутрибрюшинное введение крысам раствора адреналина-гидрохлорида (0,1 мг/кг) однократно или ежедневно в течение 3 сут эксперимента соответственно. Контролем служили крысы, получавшие физиологический раствор (в/б) в том же объеме. Буккальный эпителий исследовали на 7-е сут после введения адреналина. Мазки клеток эпителия окрашивали по Романовскому-Гимза, на препаратах подсчитывали количество клеток с микроядрами на 1000 клеток. Электрофоретическую подвижность эритроцитов оценивали методом микроэлектрофореза. Результаты. Наблюдалась качественно однотипная динамика показателя электрофоретической подвижности эритроцитов, количественно зависящая от силы воздействия. При слабых стимулах скорость изменений электрофоретической подвижности эритроцитов незначительна и сочетается с развитием адаптивных процессов, связанных с репарацией генетических повреждений. Увеличение степени стрессового воздействия путем трехкратного введения адреналина приводит к резкому изменению электрофоретической подвижности эритроцитов и росту числа эпителиоцитов с различными патологическими изменениями. Заключение. Показана возможность использования данного метода в качестве маркерной характеристики интенсивности стрессовой реакции с выявлением ее генотоксичности. Diagnostics of stress reaction using an analysis of hormonal state is a difficult, costly, and labor-consuming research. Therefore, searching for information technologies, which could be easily used in any medical laboratory and would allow evaluating the stress reaction is relevant. We studied the electrophoretic mobility of red blood cells (RBC) and buccal epithelial cells of rats exposed to stress induced by a single or repeated intraperitoneal epinephrine injection. This study showed that these methods could be used as the marker characteristics of stress reaction intensity and genotoxicity. Qualitative changes in the electrophoretic mobility of RBC were similar but quantitative characteristics depended on the acting force. Under a weak stimulus, changes in the electrophoretic mobility of RBC were slight and associated with development of adaptive processes related with repair of genetic damage. A further increase in the acting force led to a sharp change in the electrophoretic mobility of RBC and an increase in the number of epithelial cells with pathological alterations.

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