scholarly journals In vitro Growth and Differentiation of Epithelial Cells derived from Post-embryonic Hair Follicles

1982 ◽  
Vol 35 (1) ◽  
pp. 103 ◽  
Author(s):  
Dean R Hewish ◽  
Robert C Marshall
Keyword(s):  
Epithelial Cells ◽  
Electrophoretic Analysis ◽  
Hair Follicles ◽  
Culture Period ◽  
Fibroblast Cells ◽  
Cell Aggregates ◽  
In Vitro Growth ◽  
Cultured Cell ◽  
Cell Densities

Cell aggregates formed during the first 2 days of culture of cells derived from hair folIicle tissue of young rats. Aggregates occurred at an accelerated rate at higher cell densities, and contained a high proportion of epithelial cells although a variable proportion of esenchymal (fibroblast) cells were present. Citrulline was detected in the cultured cell proteins. Electrophoretic analysis of the proteins showed the presence of hair cortical keratin in the cultures, but these proteins were not synthesized during the culture period, in conflict with previous findings.

scholarly journals Interleukin 1α and interleukin 6 promote the in vitro growth of both normal and neoplastic human cervical epithelial cells

1997 ◽  
Vol 75 (6) ◽  
pp. 855-859 ◽  
Author(s):  
G Castrilli ◽  
D Tatone ◽  
MG Diodoro ◽  
S Rosini ◽  
M Piantelli ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Interleukin 6 ◽  
In Vitro Growth ◽  
Interleukin 1Α ◽  
Cervical Epithelial Cells

In vitro growth and maintenance of two morphologically distinct populations of thymic epithelial cells

1985 ◽  
Vol 90 (2) ◽  
pp. 439-450 ◽  
Author(s):  
A.C. Nieburgs ◽  
P.T. Picciano ◽  
J.H. Korn ◽  
T. McCalister ◽  
C. Allred ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
In Vitro Growth

scholarly journals Diminished in vitro growth of epidermal epithelial cells in PSS

1976 ◽  
Vol 19 (5) ◽  
pp. 969-970
Author(s):  
Michael D. Parker ◽  
Grace P. Kerby
Keyword(s):  
Epithelial Cells ◽  
In Vitro Growth

scholarly journals ANTIGEN-ANTIBODY REACTIONS IN ALLERGIC HUMAN TISSUES

1960 ◽  
Vol 112 (1) ◽  
pp. 55-64 ◽  
Author(s):  
Ben Z. Rappaport
Keyword(s):  
Epithelial Cells ◽  
Fluorescent Dyes ◽  
Fibrous Tissue ◽  
Specific Antigen ◽  
Hair Follicles ◽  
Sweat Glands ◽  
Specific Staining ◽  
Intense Staining ◽  
Egg Albumin

Skin sensitizing human antibodies were conjugated with various fluorescent dyes without significant loss in their ability to combine with specific antigen in vitro. A biopsy of the skin site challenged with egg albumin in a patient sensitive to this antigen could be stained specifically by the fluorescent reagins. The epithelial cells of the epidermis, sweat glands, hair follicles, and sebaceous glands in such a challenged site showed specific staining. In addition to the epithelial cells, the most intense staining was in macrophages and in pericapillary cells. The endothelium of the small blood vessels stained less intensely. Fibrous tissue bundles were specifically stained. The immunologic staining with the conjugated reagins was similar to but more intense than that obtained with conjugated rabbit anti-egg albumin globulins.

scholarly journals Influence of co-culture during maturation on the developmental potential of equine oocytes fertilized by intracytoplasmic sperm injection (ICSI)

Reproduction ◽  
2001 ◽  
pp. 925-932 ◽  
Author(s):  
X Li ◽  
LH Morris ◽  
WR Allen
Keyword(s):  
Epithelial Cells ◽  
Intracytoplasmic Sperm Injection ◽  
Fibroblast Cells ◽  
Cell Stage ◽  
Developmental Potential ◽  
Fetal Fibroblast ◽  
Nuclear Maturation ◽  
Fetal Fibroblasts ◽  
Oviduct Epithelial Cells

The influence of co-culture with either oviduct epithelial cells or fetal fibroblast cells on in vitro maturation of equine oocytes and their potential for development to blastocysts and fetuses after intracytoplasmic sperm injection (ICSI) was investigated. The oocytes were obtained from ovaries from abattoirs and were matured in vitro for 28-30 h in TCM-199 only, or in TCM-199 co-culture with oviduct epithelial cells or fetal fibroblast cells. Metaphase II oocytes were subjected to ICSI with an ionomycin-treated spermatozoon. The injected oocytes were cultured for 7-9 days in Dulbecco's modified Eagle's medium. Morphologically normal early blastocysts were transferred to the uteri of recipient mares. Nuclear maturation rates and the rates of cleavage to the two-cell stage for injected oocytes were similar in the groups of oocytes that were matured in TCM-199 (49 and 63%), in co-culture with oviduct epithelial cells (53 and 65%) or in co-culture with fetal fibroblasts (51 and 57%). There were no significant differences in the proportions of blastocysts that developed from the two-cell embryos derived from oocytes matured by co-culture with either oviduct epithelial cells (30%) or fetal fibroblasts (17%). However, significantly higher proportions of blastocysts were produced from both these co-culture groups than from the groups of oocytes matured in TCM-199 only (P < 0.05). Six of the blastocysts that had developed from oocytes co-cultured with oviduct epithelial cells were transferred into recipient mares and four pregnancies resulted. These results demonstrate a beneficial influence of co-culture with either oviduct epithelial cells or fetal fibroblasts for maturation of oocytes in vitro.

scholarly journals Production of GJycosaminoglycans by Rat Hair Follicle Cells in Vitro

1981 ◽  
Vol 34 (2) ◽  
pp. 171 ◽  
Author(s):  
R Frater ◽  
D Hewish
Keyword(s):  
Hyaluronic Acid ◽  
Heparan Sulfate ◽  
Hair Follicle ◽  
Culture Medium ◽  
Dermatan Sulfate ◽  
Hair Follicles ◽  
Follicle Cells ◽  
Cell Aggregates ◽  
Dermal Tissue

Cultures of cells from rat dermal tissue, containing a large proportion of cells from the hair follicles, were found to produce glycosaminoglycans. The glycans were associated with the cell aggregates which typically form in such cultures, and also appeared to be present in material which was released from the cells. Examination of the glycosaminoglycan species present in the cultures showed the presence of hyaluronic acid, dermatan sulfate, chondroitin-4-sulfate, heparin and heparan sulfate-C, the first two compounds being secreted.into the culture medium. The patterns of synthesis and sulfation of glycosaminoglycans were found to change with continued time in culture.

90 CHARACTERIZATION OF A PRIMARY CULTURE OF OVIDUCTAL CELLS IN THE BITCH

2014 ◽  
Vol 26 (1) ◽  
pp. 159
Author(s):  
C. Gibson ◽  
K. Reynaud ◽  
S. Thoumire ◽  
B. Grimard ◽  
M. Saint-Dizier
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Primary Culture ◽  
Culture Period ◽  
Stable Gene ◽  
Mucosal Cell ◽  
Fibroblast Cells ◽  
Mrna Levels ◽  
Ciliated Cells ◽  
Cell Functions

The oviduct is of particular importance in canine reproduction as it supports oocyte meiosis resumption, sperm capacitation and storage, fertilization and embryo development to the morula/blastocyst stage for 8 to 10 days post-ovulation. A long-time co-culture with oviducal cells could be employed to improve the yield of reproductive biotechnologies in this species, but no characterisation of canine oviduct cells in vitro has been reported to date. The objectives of this study were to (1) evaluate the viability and proportion of epithelial/fibroblast cells in a primary culture of canine oviducal cells collected around ovulation; (2) study the responsiveness of the cultured cells to steroids. Beagle bitches (n = 9) were ovariectomized between Day –1 and Day +1 around ovulation, and their oviducts were sectioned at the ampulla-isthmus junction. Mucosal cells (including stromal and epithelial cells) were collected by squeezing from the ampulla and isthmus sections and cultured separately at a concentration of 5 × 105 cells/well in 500 μL of M199 + 10% FCS at 39°C for 9 days. At Days 3 and 6, 1 × 106 cells were stimulated with 17β-oestradiol (E2, 20 pg mL–1) or progesterone (P4, 20 ng mL–1) for 6 h. At Days 3, 6, and 9 of culture, the viability of the cells was evaluated using the Live/Dead kit (Invitrogen, Carlsbad, CA, USA), and proportions of fibroblast and epithelial ciliated cells were evaluated by immuno-cytochemistry using anti-vimentin and anti-tubulin antibodies, respectively. At Days 0, 3 and 6, the total RNA was extracted from cells and mRNA levels of the oviduct-specific glycoprotein (OVGP, synthesised by nonciliated epithelial cells), E2 (ERα, ERβ) and P4 (PR) receptors were evaluated by RT-qPCR. The effects of the day of culture and of steroid exposure on mRNA levels were analysed by ANOVA followed by a Tukey test. Cell confluence was observed around Day 6 of culture and more than 90% of cells survived during the 9-day culture period. From Day 3 to Day 9, the proportion of vimentin-positive (fibroblast) cells was greater than 68% in both ampulla and isthmus cells. In contrast, the proportion of epithelial ciliated cells was low at Day 3 (9% in ampulla, 12% in isthmus) and null at Days 6 and 9 in both regions. The mRNA levels of OVGP, ER, and PR decreased significantly after 3 days of culture, and then remained stable in both ampulla and isthmus cells (P < 0.001). The steroid exposure had no effect on gene expression, except for ERα mRNA levels at Day 3, which was increased by E2 and reduced by P4 (P < 0.05). In conclusion, the method of collection did not allow us to collect a high proportion of epithelial oviducal cells. However, the relatively stable gene expression of PR and ER during the culture period provides us with a useful tool to study the steroid regulation of canine oviduct mucosal cell functions.

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