scholarly journals Diminished in vitro growth of epidermal epithelial cells in PSS

1976 ◽  
Vol 19 (5) ◽  
pp. 969-970
Author(s):  
Michael D. Parker ◽  
Grace P. Kerby
Keyword(s):  
Epithelial Cells ◽  
In Vitro Growth

scholarly journals Interleukin 1α and interleukin 6 promote the in vitro growth of both normal and neoplastic human cervical epithelial cells

1997 ◽  
Vol 75 (6) ◽  
pp. 855-859 ◽  
Author(s):  
G Castrilli ◽  
D Tatone ◽  
MG Diodoro ◽  
S Rosini ◽  
M Piantelli ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Interleukin 6 ◽  
In Vitro Growth ◽  
Interleukin 1Α ◽  
Cervical Epithelial Cells

In vitro growth and maintenance of two morphologically distinct populations of thymic epithelial cells

1985 ◽  
Vol 90 (2) ◽  
pp. 439-450 ◽  
Author(s):  
A.C. Nieburgs ◽  
P.T. Picciano ◽  
J.H. Korn ◽  
T. McCalister ◽  
C. Allred ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
In Vitro Growth

scholarly journals In vitro Growth and Differentiation of Epithelial Cells derived from Post-embryonic Hair Follicles

1982 ◽  
Vol 35 (1) ◽  
pp. 103 ◽  
Author(s):  
Dean R Hewish ◽  
Robert C Marshall
Keyword(s):  
Epithelial Cells ◽  
Electrophoretic Analysis ◽  
Hair Follicles ◽  
Culture Period ◽  
Fibroblast Cells ◽  
Cell Aggregates ◽  
In Vitro Growth ◽  
Cultured Cell ◽  
Cell Densities

Cell aggregates formed during the first 2 days of culture of cells derived from hair folIicle tissue of young rats. Aggregates occurred at an accelerated rate at higher cell densities, and contained a high proportion of epithelial cells although a variable proportion of esenchymal (fibroblast) cells were present. Citrulline was detected in the cultured cell proteins. Electrophoretic analysis of the proteins showed the presence of hair cortical keratin in the cultures, but these proteins were not synthesized during the culture period, in conflict with previous findings.

Factors Affecting in Vitro Growth of Harvested Enterocytes

1992 ◽  
Vol 1 (4) ◽  
pp. 299-306 ◽  
Author(s):  
M. Santos ◽  
B. T. Nguyen ◽  
J.S. Thompson
Keyword(s):  
Epithelial Cells ◽  
Cell Viability ◽  
Cell Number ◽  
Cell Yield ◽  
Growth Media ◽  
In Vitro Growth ◽  
Factors Affecting ◽  
Initial Cell ◽  
Number Of Cells

Selective enterocyte transplantation may be an alternative to whole organ transplantation for increasing absorptive capacity. Our aim was to determine the effect of initial cell number and viability, proportion of intact crypts, and basement membrane components (BMC) on the in vitro growth of rabbit enterocytes. Enterocytes were harvested using warm trypsinization from ileal segments in 40 rabbits. Initial cell viability was 92 ± 4% (mean ± SD), cell yield was 7.7 ± 3.6 × 106 cells/cm, and there were 0.51 ± 0.33 crypts/100 cells. Initial cell viability correlated with cell yield (r = −0.508, p < 0.001) and % crypts (r = 0.313, p < 0.05). Cell yield also correlated with % crypts (r = −0.645, p < 0.001). Enterocytes (5 × 106) were incubated in growth media in plain or BMC coated growth culture vessels for 14 days. There was a correlation between both number of cells seeded (r = 0.824, p < 0.001) and cell viability (r = −0.696, p < 0.01) and % growth colonies containing epithelial cells at 14 days. Both total growth colonies (r = −0.565, p < 0.05) and colonies with epithelial cells (r = −0.589, p < 0.05) had a negative correlation with % crypts. Incubating cells in BMC coated vessels (n = 6) resulted in significantly more dispase liberated cells after 14 days than in plain vessels (n = 6) (6.6 ± 1.1 vs. 3.8 ± 1.0 × 106, p < 0.05) but viability was similar (97 ± 2% vs. 96 ± 2%). Diamine oxidase (DAO) activity was significantly increased in the growth media of cells cultured on BMC compared to controls (0.76 ± 0.24 vs. 0.43 ± 0.28 units, p < 0.05) although DAO act/cell was similar. Conclusions: 1) cell yield using warm trypsinization is increased at the expense of viability and intact crypts; 2) in vitro growth is increased by increasing the number of cells but not intact crypts seeded; 3) BMC increase the yield of cells with viability and maturity similar to those cultured in plain vessels.

Pentagastrin stimulates in vitro growth of normal and malignant human colon epithelial cells

1985 ◽  
Vol 149 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Kenneth R. Sirinek ◽  
Barry A. Levine ◽  
Mary Pat Moyer
Keyword(s):  
Epithelial Cells ◽  
Human Colon ◽  
In Vitro Growth

scholarly journals Evaluation of silk sericin as a biomaterial: in vitro growth of human corneal limbal epithelial cells on Bombyx mori sericin membranes

2013 ◽  
Vol 2 (1) ◽  
pp. 14 ◽  
Author(s):  
Traian V Chirila ◽  
Shuko Suzuki ◽  
Laura J Bray ◽  
Nigel L Barnett ◽  
Damien G Harkin
Keyword(s):  
Epithelial Cells ◽  
Bombyx Mori ◽  
In Vitro Growth ◽  
Silk Sericin
Sign in / Sign up

Export Citation Format

Share Document