scholarly journals Extension of the culture period for the in vitro growth of bovine oocytes in the presence of bone morphogenetic protein-4 increases oocyte diameter, but impairs subsequent developmental competence

2017 ◽  
Vol 88 (11) ◽  
pp. 1686-1691 ◽  
Author(s):  
Yinghua Yang ◽  
Chihiro Kanno ◽  
Kenichiro Sakaguchi ◽  
Yojiro Yanagawa ◽  
Seiji Katagiri ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Developmental Competence ◽  
Culture Period ◽  
Bone Morphogenetic Protein 4 ◽  
Oocyte Diameter ◽  
In Vitro Growth ◽  
Morphogenetic Protein ◽  
Bovine Oocytes

scholarly journals Evidence for involvement of activin A and bone morphogenetic protein 4 in mammalian mesoderm and hematopoietic development.

1995 ◽  
Vol 15 (1) ◽  
pp. 141-151 ◽  
Author(s):  
B M Johansson ◽  
M V Wiles
Keyword(s):  
Growth Factor ◽  
Bone Morphogenetic Protein ◽  
Activin A ◽  
Bone Morphogenetic Protein 4 ◽  
Mesoderm Induction ◽  
Dependent Manner ◽  
Tgf Beta ◽  
Mouse Development ◽  
Morphogenetic Protein

Xenopus in vitro studies have implicated both transforming growth factor beta (TGF-beta) and fibroblast growth factor (FGF) families in mesoderm induction. Although members of both families are present during mouse mesoderm formation, there is little evidence for their functional role in mesoderm induction. We show that mouse embryonic stem cells, which resemble primitive ectoderm, can differentiate to mesoderm in vitro in a chemically defined medium (CDM) in the absence of fetal bovine serum. In CDM, this differentiation is responsive to TGF-beta family members in a concentration-dependent manner, with activin A mediating the formation of dorsoanterior-like mesoderm and bone morphogenetic protein 4 mediating the formation of ventral mesoderm, including hematopoietic precursors. These effects are not observed in CDM alone or when TGF-beta 1, -beta 2, or -beta 3, acid FGF, or basic FGF is added individually to CDM. In vivo, at day 6.5 of mouse development, activin beta A RNA is detectable in the decidua and bone morphogenetic protein 4 RNA is detectable in the egg cylinder. Together, our data strongly implicate the TGF-beta family in mammalian mesoderm development and hematopoietic cell formation.

scholarly journals Stimulation of Epithelial Sodium Channels in Endothelial Cells by Bone Morphogenetic Protein-4 Contributes to Salt-Sensitive Hypertension in Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Xu Yang ◽  
Na Niu ◽  
Chen Liang ◽  
Ming-Ming Wu ◽  
Liang-Liang Tang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Bone Morphogenetic Protein ◽  
Salt Intake ◽  
High Salt ◽  
Bone Morphogenetic Protein 4 ◽  
Sodium Influx ◽  
Morphogenetic Protein ◽  
High Salt Intake ◽  
Stimulation Of

Previous studies have shown that high salt induces artery stiffness by causing endothelial dysfunction via increased sodium influx. We used our unique split-open artery technique combined with protein biochemistry and in vitro measurement of vascular tone to test a hypothesis that bone morphogenetic protein 4 (BMP4) mediates high salt-induced loss of vascular relaxation by stimulating the epithelial sodium channel (ENaC) in endothelial cells. The data show that high salt intake increased BMP4 both in endothelial cells and in the serum and that exogenous BMP4 stimulated ENaC in endothelial cells. The data also show that the stimulation is mediated by p38 mitogen-activated protein kinases (p38 MAPK) and serum and glucocorticoid-regulated kinase 1 (Sgk1)/neural precursor cell expressed developmentally downregulated gene 4-2 (Nedd4-2) (Sgk1/Nedd4-2). Furthermore, BMP4 decreased mesenteric artery relaxation in a benzamil-sensitive manner. These results suggest that high salt intake stimulates endothelial cells to express and release BMP4 and that the released BMP4 reduces artery relaxation by stimulating ENaC in endothelial cells. Therefore, stimulation of ENaC in endothelial cells by BMP4 may serve as another pathway to participate in the complex mechanism of salt-sensitive (SS) hypertension.

scholarly journals Regulation of Pancreatic α-Cell Function and Proliferation by Bone Morphogenetic Protein 4 (BMP4) In Vitro

Endocrinology ◽  
2016 ◽  
Vol 157 (10) ◽  
pp. 3809-3820 ◽  
Author(s):  
Sofie Sylvest Nielsen ◽  
Gitte Lund Christensen ◽  
Jens Juul Holst ◽  
Nils Billestrup
Keyword(s):  
Bone Morphogenetic Protein ◽  
Cell Function ◽  
Bone Morphogenetic Protein 4 ◽  
Morphogenetic Protein

Bone morphogenetic protein 4 induces efficient hematopoietic differentiation of rhesus monkey embryonic stem cells in vitro

Blood ◽  
2001 ◽  
Vol 98 (2) ◽  
pp. 335-342 ◽  
Author(s):  
Fei Li ◽  
Shijiang Lu ◽  
Loyda Vida ◽  
James A. Thomson ◽  
George R. Honig
Keyword(s):  
Rhesus Monkey ◽  
Bone Morphogenetic Protein ◽  
Culture System ◽  
Es Cells ◽  
Regulatory Protein ◽  
Embryonic Stem ◽  
Bone Morphogenetic Protein 4 ◽  
Hematopoietic Differentiation ◽  
Morphogenetic Protein

A cell culture system consisting of mouse S17 stromal cells supplemented with cytokines was developed for hematopoietic differentiation of rhesus monkey embryonic stem (ES) cells. The differentiated colonies that formed contained clusters of hematopoietic-like cells, as well as structures similar in appearance to embryonic blood islands. When this culture system was supplemented with bone morphogenetic protein 4 (BMP-4), the numbers of primary hematopoietic clusters increased by an average of 15 fold. The primary hematopoietic clusters containing clonogenic precursors (expandable hematopoietic clusters) increased by 18 fold. Immunofluorescence analysis showed that a substantial percentage of the hematopoietic-like cells were CD34+, with morphologic features of undifferentiated blast cells. Enrichment of the CD34+ cells was associated with enhanced stromal-dependent, cytokine-driven formation of cobblestone colonies on secondary plating. The hematopoietic identity of the precursors was further indicated by their expression of genes associated with hematopoietic differentiation, as well as morphologic assessments that showed erythroid and myeloid lineages among the progeny cells. In addition, reverse transcriptase–polymerase chain reaction analysis of BMP-4–treated rhesus monkey ES cells demonstrated an up-regulation of early-expressed genes responsible for embryonic hematopoiesis and angiogenesis during the first 7 days of culture. These observations suggest that embryonic mesoderm regulatory protein may mimic physiologic signals that are required for the onset of embryonic hematopoiesis and stem cell formation in rhesus monkey ES cells.

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