scholarly journals Degradation of Nucleic Acid by Allomyces macrogynus during the Production of Zoosporangia and Resistant Sporangia

1980 ◽  
Vol 33 (3) ◽  
pp. 393 ◽  
Author(s):  
Jean Youatt
Keyword(s):  
Nucleic Acid ◽  
Acid Solution ◽  
Glutamic Acid ◽  
Inorganic Phosphate ◽  
Distilled Water ◽  
Allomyces Macrogynus ◽  
Purines And Pyrimidines

Allomyces macrogynus plants were induced to make either zoosporangia in distilled water or resistant sporangia in a solution of glucose and glutamic acid. Analyses during the stages of developmentJ showed that plants of both series degraded nucleic acid, releasing uracil, hypoxanthine and guanin~ to the suspending medium. Plants in distilled water released inorganic phosphate to the medium while those in glucose-glutamic acid solution conserved the phosphate as bound phosphate. A. arbuscula also released the purines and pyrimidines.

scholarly journals Changes in the Carbohydrates of Allomyces Macrogynus During the Selective Development of Either Zoosporangia or Resistant Sporangia

1980 ◽  
Vol 33 (4) ◽  
pp. 505 ◽  
Author(s):  
Jean Youatt
Keyword(s):  
Nucleic Acid ◽  
Glutamic Acid ◽  
Glucose Solution ◽  
Allomyces Macrogynus

Allomyces macrogynus synthesized trehalose but did not appear to metabolize it. Trehalose was released to the suspending medium before the development of zoosporangia by plants suspended in water and before the development of resistant sporangia by plants in glutamic acid and glucose solution. Plants in the latter solution accumulated high levels of glycogen and trehalose before mRNA was transcribed for the development of resistant sporangia. Before the transcription of mRNA for either zoosporangia or resistant sporangia there was a period during which the plant content of glycogen, trehalose, protein and nucleic acid was constant. The increase in wall glycans and chitin during the development of zoosporangia was measured and possible origins of the walls are discussed.

scholarly journals THE EFFECT OF LACTIC ACID AND ARTIFICIAL SALIVA SOLUTION IMMERSION TO THE RELEASE OF CALCIUM IONS ON BIOACTIVE RESIN

2021 ◽  
Vol 6 (2) ◽  
pp. 190
Author(s):  
Dewi Puspitasari ◽  
Nurah Tajjalia ◽  
Diana Wibowo ◽  
Agung Satria Wardhana
Keyword(s):  
Lactic Acid ◽  
Acid Solution ◽  
Calcium Ions ◽  
Calcium Ion ◽  
Artificial Saliva ◽  
Distilled Water ◽  
Acid Condition ◽  
Ion Release ◽  
Lactic Acid Solution ◽  
Solution Increase

Background: Bioactive resin can release calcium ions when contact with solution media, even in acid condition. In the oral cavity, pH may change into acid condition due to the metabolic results of Streptococcus mutans. The bacteria metabolize carbohydrates into organic acids, one of which is lactic acid. Purpose: Analyze the effect of lactic acid solution and artificial saliva on the number of the release of calcium ions of bioactive resin. Methods: Forty-two specimens (diameter 15 mm x thickness 1 mm; n= 7/group fabricated with Activa™ Bioactive Restorative (Pulpdent). The specimens that meet the criteria were divided into 6 groups. The specimen was immersed for 1 and 7 days in the incubator at 37oC. The number of calcium ion release is measured using titration method. Results: Two Way Anova test and Post Hoc Bonferonni test showed there were significant differences among all group for lactic acid 1 day (4.040 ± 0.360) µg, artificial saliva 1 day (0.640 ± 0.338) µg, distilled water 1 day (1.040 ± 0.504) µg, lactic acid 7 days (5.400 ± 0.312), artificial saliva 7 days (1.640 ± 0.215) µg, distilled water 7 days (3.520± 0.356 µg). Conclusion: There was an influence of lactic acid and artificial saliva on the number of calcium ion releases of bioactive resin. Immersion of bioactive resin in the lactic acid solution increase the calcium ion releases and artificial saliva decrease the calcium ion release compared to distilled water. Keywords: artificial saliva, bioactive resin, calcium ion release, lactic acid

THE SEPARATION OF CALF THYMUS NUCLEIC ACID AND HISTONE BY ALCOHOL–SALT PRECIPITATION

1955 ◽  
Vol 33 (1) ◽  
pp. 263-271 ◽  
Author(s):  
L. B. Smillie ◽  
A. M. Marko ◽  
G. C. Butler
Keyword(s):  
Sodium Chloride ◽  
Nucleic Acid ◽  
Aqueous Solutions ◽  
Distilled Water ◽  
Salt Precipitation ◽  
Salt Solutions ◽  
Desoxyribonucleic Acid ◽  
Concentrated Aqueous Solutions ◽  
Calf Thymus ◽  
Quantitative Separation

Extraction of the histone of thymonucleoprotein with alcohol and salt has been studied with varying concentrations of alcohol, sodium chloride, and nucleoprotein and with varying pH and temperature. The addition of 0.5–1.0 volumes of ethanol to a solution of nucleoprotein (approximately 1 mgm. N/ml.) in 3 M sodium chloride has been found to effect an almost quantitative separation of desoxyribonucleic acid and protein. It has proved feasible to prepare concentrated aqueous solutions of the extracted protein by successive dialysis against strong salt solutions at −10 °C. and against distilled water at 5 °C. followed by pervaporation at 5 °C. The properties of the isolated nucleate have indicated a highly polymerized product.

The Effect of Linuron on 32P and 45Ca Uptake in Tomato and Parsnip

Weed Science ◽  
1968 ◽  
Vol 16 (2) ◽  
pp. 185-187 ◽  
Author(s):  
E. J. Hogue
Keyword(s):  
Nucleic Acid ◽  
Inorganic Phosphate ◽  
Culture Solution ◽  
Lycopersicum Esculentum ◽  
Pastinaca Sativa ◽  
Tomato Plants ◽  
45Ca Uptake ◽  
Nutrient Culture

The effects of sublethal and lethal levels of 3-(3,4-dichlorophenyl)-l-methoxy-l-methylurea (linuron) on the uptake of 32P and 45Ca were tested on tomato (Lycopersicum esculentum Mill., var. Kokomo) and parsnip (Pastinaca sativa L., var. Harris Model). Both levels of foliage-applied linuron stimulated the uptake of 32P from nutrient culture solution and its translocation to the leaves. The increased 32P in the leaves of the treated tomato plants was found in the inorganic phosphate fraction. Incorporation of the label in the phospholipid and nucleic acid fractions was decreased slightly. Linuron inhibited uptake and translocation of 45Ca in both plants.

scholarly journals The Effect of Periogen Solution on Dental Calculus in vitro: A Pilot Study

2017 ◽  
Vol 6 (1) ◽  
pp. 33-34
Author(s):  
Marta Radnai ◽  
Zsolt Rajnics
Keyword(s):  
Pilot Study ◽  
Hydrochloric Acid ◽  
Acid Solution ◽  
Oral Hygiene ◽  
Direct Contact ◽  
Photometric Method ◽  
Distilled Water ◽  
Dental Calculus ◽  
Carbamide Peroxide

ABSTRACT Introduction Calculus can accommodate teeth and prosthetic restorations when the patient's oral hygiene is poor. Hardened calculus cannot be removed by patients, it needs professional cleaning using ultrasonic scaler or hand instrument. Solutions dissolving and preventing accumulation of dental calculus may help to keep dentures clean. The aim of this investigation was to examine the effect of Periogen on dental calculus in vitro. Materials and methods Calculus was collected via scaling from patients during a routine dental checkup. The samples were stored in carbamide peroxide solution (5%) for 24 hours, then rinsed and stored in distilled water. First, the Ca2+ content of the calculus was determined by photometric method after treating with cc. hydrochloric acid solution for 1 hour. The calculus samples were put in Periogen solution, prepared according to manufacturer's instruction for 16 hours, then crushed and put in Periogen solution with the same concentrate for 4.5 hours. The Ca2+ dissolved from calculus was measured using same photometric method. Results Calculus samples contained 26 mg/100 mg Ca2+, which is similar to dentin (27–28 mg/100 mg) and to enamel (36 mg/100 mg). The Ca2+ dissolved from calculus after treating with cc. hydrochloric acid for 1 hour was considered 100%. Ca2+ dissolution was 1.5% after 16 hours (0.09%/h) and 5.45% (1.21%/h) for the next 4.5 hours after pulverizing the sample. This showed Periogen Ca2+ dissolution was 385 µg/100 mg after 16 hours direct contact with the material. Conclusion The experiment showed that pulverizing the previously hard calculus was done easily after soaking it in Periogen. The ability of Periogen to soften the calculus needs to be further investigated. How to cite this article Rajnics Z, Radnai M. The Effect of Periogen Solution on Dental Calculus in vitro: A Pilot Study. Int J Experiment Dent Sci 2017;6(1):33-34.

Synthesis of Gold Nanoparticles Using Glutamic Acid as a Reductor and Capping Agent

2020 ◽  
Vol 840 ◽  
pp. 472-477
Author(s):  
Naura Nadhifah ◽  
Wening Rara Pratita ◽  
Eko Sri Kunarti ◽  
Nuryono Nuryono ◽  
Sri Juari Santosa
Keyword(s):  
Gold Nanoparticles ◽  
Acid Solution ◽  
Glutamic Acid ◽  
Color Change ◽  
Reaction Times ◽  
Chloroauric Acid ◽  
Optimum Synthesis ◽  
Transmission Electron ◽  
Particle Size Analyzer ◽  
Uv Visible

The synthesis of gold nanoparticles (AuNPs) was carried out by reacting chloroauric acid [AuCl4]‒ and glutamic acid solution in a water bath at 80 °C. The reaction was confirmed by the color change from colorless solution into pink. Various concentrations of the glutamic acid solution, reaction times and pH were examined to determine the optimum condition of AuNPs synthesis. The AuNPs colloid was characterized by UV-Visible spectrophotometer at wavelength 350-800 nm, particle size analyzer (PSA) and transmission electron microscope (TEM). The optimum synthesis condition for obtained [AuCl4]‒ was 4 mM glutamic acid solution at pH 11 and 60 min reaction. TEM results showed that the particles were round in shape with the article size indicated by PSA was 40 nm.

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