scholarly journals Maintenanance of Viability of Carrot Tissue Slices in Washing Solutions After Cutting

1971 ◽  
Vol 24 (2) ◽  
pp. 397 ◽  
Author(s):  
RA Wildes ◽  
TF Neales
Keyword(s):  
Biological Activity ◽  
Boric Acid ◽  
Calcium Chloride ◽  
Respiration Rate ◽  
Bacterial Contamination ◽  
Plant Tissues ◽  
Tissue Slices ◽  
Storage Tissue ◽  
Physiological Studies

In studies of the characteristics of the uptake of boric acid by plant tissues (Wildes and Neales, unpublished data) we used disks cut from carrot roots. Bacon, MacDonald, and Knight (1965) have emphasized the necessity of using storage tissue free of bacteria for such physiological studies. In the course of our experiments we therefore investigated the extent of bacterial contamination in carrot disks immediately after cutting, and also examined the effects of chloramphenicol and calcium chloride in the washing solution on the extent of the development of bacterial contamination in the disks. We also made measurements of various properties that gave evidence of their normal biological activity. The loss of this activity, in our experience, was characterized by a final loss of turgor, browning of both tissue and washing solutions, and a failure to respond in respiration rate to both the addition of salts and 2,4-dinitrophenol.

Extraction, Gas-Liquid Chromatographic Detection, and Quantitation of Hexachlorophene Residues from Plant Tissues High in Lipid Content

1977 ◽  
Vol 60 (5) ◽  
pp. 1087-1092
Author(s):  
Oscar W Van Auken ◽  
Michael Hulse ◽  
Constance L Durocher
Keyword(s):  
Silica Gel ◽  
Lipid Content ◽  
Neutral Lipids ◽  
Parent Compound ◽  
Plant Tissues ◽  
Storage Tissue ◽  
Glass Column ◽  
Chromatographic Detection ◽  
Liquid Chromatographic ◽  
Plant Storage

Abstract A method has been developed for the extraction, cleanup, derivatization, detection, and quantitation of hexachlorophene (HCP) residues from 2 types of plant storage tissue high in lipid content. Wet soybean or peanut tissue was homogenized and extracted with ethyl ether and chromatographed on silica gel to remove the neutral lipids. The cleaned up sample was methylated with diazomethane and the dimethoxyhexachlorophene was eluted from a second silica gel column and chromatographed on a 6′ glass column packed with 3% OV-1 or 3% SE-30 on Gas-Chrom Q. The instrument detection limit for the 63Ni electron capture detector was <0.1 ng for dimethoxyhexachlorophene and about 1 ppb for HCP residue in plant issue. Recovery of 10–420 ppb HCP added to tissue averaged 90.9±5.7%. Interfering substances were removed, column life was increased, peak sharpness was increased, and tailing of the parent compound was decreased by using appropriate column chromatography.

Bacterial contamination of in vitro plant cultures: confounding effects on somaclonal variation and detection of contamination in plant tissues

2014 ◽  
Vol 119 (3) ◽  
pp. 533-541 ◽  
Author(s):  
Santiago Moreno-Vázquez ◽  
Nerea Larrañaga ◽  
Elizabeth C. Uberhuaga ◽  
Eugenia Jacira Bolacel Braga ◽  
César Pérez-Ruíz
Keyword(s):  
Somaclonal Variation ◽  
Bacterial Contamination ◽  
Plant Tissues ◽  
In Vitro Plant

scholarly journals Ethylene Production by Slices of Green Banana Fruit and Potato Tuber Tissue During The Development of Induced Respiration

1969 ◽  
Vol 22 (2) ◽  
pp. 489 ◽  
Author(s):  
WB Mcglasson
Keyword(s):  
Ethylene Production ◽  
Plant Tissues ◽  
Underground Storage ◽  
Banana Fruit ◽  
Physiological Changes ◽  
Tissue Slices ◽  
Tuber Tissue ◽  
Plant Parts ◽  
Storage Organs ◽  
Green Banana

It is well known that injury and infection by disease organisms may stimulate ethylene production by plant tissues (Williamson 1950; Burg 1962; McGlasson and Pratt 1964). The increased ethylene production which results from injury in fruit tissues may hasten the onset of a respiratory climacteric. This response, which has been observed in slices cut from three-quarter-grown cantaloupe fruit, may herald the commencement of physiological changes leading to natural ripening (McGlasson and Pratt 1964). However, in underground storage tissues, stimulated ethylene production may be concerned with the mechanisms of wound healing (Stahmann, Clare, and Woodbury 1966; Imaseki, Uchiyama, and Uritani 1968). The phenomenon of induced respiration in tissue slices of bulky underground storage organs has been known for many years (Laties 1967) and more recently it has been found to occur in sections or slices of other plant parts (ap Rees 1966). Palmer and McGlasson (1969) observed a similar rise in slices of green banana fruit which they considered to be a form of "induced" respiration.

Вплив обробітку ґрунту та мінеральних добрив на біологічну активність ґрунту під посівами кукурудзи цукрової

2017 ◽  
Vol 7 (4) ◽  
pp. 18-24 ◽  
Author(s):  
P. V. Lykhovyd ◽  
S. O. Lavrenko
Keyword(s):  
Biological Activity ◽  
Respiration Rate ◽  
Field Experiments ◽  
Irrigation System ◽  
Application Rate ◽  
Soil Microflora ◽  
Mineral Fertilizers ◽  
Soil Biological Activity ◽  
Experimental Variant ◽  
Tillage Depth

<p>The article is devoted to study of the biological activity of soil under sweet corn crops depending on tillage depth, mineral fertilizers application rate with drip irrigation in the soil-ecological conditions of the Dry Steppe Zone of Ukraine. The plan of the experiment foresaw study of such factors: A — tillage depth (moldboard plowing on 20-22 and 28-30 cm); B — mineral fertilizers application rate (no fertilizers, N<sub>60</sub>P<sub>60</sub>, N<sub>120</sub>P<sub>120</sub>); C — plants density (35, 50, 65, 80 plants ha<sup>-1</sup>). We used water of the Ingulets irrigation system for irrigation. The water belongs to II quality class by agronomical criteria of the DSTU 2730-94 and its suitability for irrigation is limited. We studied the soil biological activity on variants with 65 plants ha<sup>-1 </sup>by the indicies of its respiration rate (method of V.I. Shtatnov) and linen decomposition degree (“application” method). At the same time we have made monitoring of the soil meliorative state  depending on the moldboard plowing depth by the toxic salts content, anionic-cationic composition of the aqueous soil extract. The results of the laboratory-field experiments prove that the moldboard plowing depth , mineral fertilizers application rates and soil meliorative state have an influence on its biological activity. The maximum respiration rate, which is used to characterize an activity of the aerobic microflora of the upper soil layers, was at moldboard plowing on depth of 20-22 cm at non-fertilized experimental variant — 250.0 mg СО<sub>2</sub>/m<sup>2</sup>×hour. Increase of the tillage depth and mineral fertilizers application rate significantly decreased carbonic acid emission to the athmosphere, which was the minimum of 178.3 mg СО<sub>2</sub>/m<sup>2</sup>×h. The maximum linen decomposition degree, which is used to characterize an activity of the cellulose-decomposing soil microflora, was at moldboard plowing depth of 28-30 cm at non-fertilized experimental variant — 58.3%. The tillage depth changed cellulose-decomposing soil microflora activity insignificantly. Mineral fertilizers application at the maximum rate of N<sub>120</sub>P<sub>120</sub>decreased cellulose-decomposing soil biota activity 1.7 times comparatively to non-fertilized variant. Higher toxic salts concentration (at 0.006-0.019 %), sodium ions content (at 0.15-0.38 mg-eq/100 g of soil) in the 0-50 cm layer led to decrease of the soil biological activity. We consider, that the best from the agroecological point of view is the variant with moldboard plowing depth of 28-30 cm, mineral fertilizers application rate N<sub>120</sub>P<sub>120</sub>.</p>

Effect of foliar spray of calcium chloride and boric acid on shelf-life of guava

2017 ◽  
Vol 74 (4) ◽  
pp. 586
Author(s):  
Anil Kumar ◽  
Navin Singh ◽  
K.K. Misra ◽  
Vishal Nirgude
Keyword(s):  
Boric Acid ◽  
Shelf Life ◽  
Calcium Chloride ◽  
Foliar Spray

scholarly journals Effect of benzyl adenine on the in vitro culture of young female flowers of Breadfruit (Artocarpus altilis (Park.) Fosberg)

2016 ◽  
Vol 19 (3) ◽  
pp. 32-42
Author(s):  
Suong Thi Tuyet Ha ◽  
Mai Thi Bach Vo
Keyword(s):  
Biological Activity ◽  
In Vitro Culture ◽  
Respiration Rate ◽  
Young Female ◽  
High Yield ◽  
Physiological Changes ◽  
Benzyl Adenine ◽  
Artocarpus Altilis ◽  
Female Flowers

Breadfruit (Artocarpus altilis (Park.) Fosberg) is a woody tropical tree. In addition to the nutritious value, breadfruit is also a precious medicinal source, because every part of it contains many natural compounds with biological activity. With the aim of providing a pharmaceutical source cond food with high yield, as well as the short harvert time, we examined the effects of the concentration of benzyl adenine (BA) on the in vitro culture of young female breadfruit flowers. The results showed that in the first phase of the culture, the concentration of 5 mg/L BA stimulated an increase in size of each female flowers, especially in the calyx. The increasing concentration of 10 mg/L BA stimulated the formation of callus in the calyx of each female flowers. Roles of respiration rate and endogenus hormones were discussed to understand the physiological changes in the in vitro culture of young female breadfruit flowers.

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