scholarly journals Effect of Time of Insemination on the Distribution of Spermatozoa in the Genital Tract in Ewes

1969 ◽  
Vol 22 (5) ◽  
pp. 1283 ◽  
Author(s):  
PE Mattner ◽  
AWH Braden

Merino ewes were artificially inseminated during either early or late oestrus and were killed 4 or 24 hr after insemination. Insemination during late oestrus resulted in fewer spermatozoa in the cervix, the uterus, and the fallopian tubes and in a lower fertilization rate. Increased resistance of the cervical mucus to penetration by spermatozoa during late oestrus appeared to be responsible for these effects.

1971 ◽  
Vol 11 (52) ◽  
pp. 478
Author(s):  
JR Giles

A series of studies was conducted in 1968 and 1969 to compare fertilization rates, return rate, and sperm distribution in the genital tract in Bungaree and Peppin Merino ewes. Differences in lambing rates of the two ewe strains were demonstrated, but there was no major difference in fertilization rate in either year. A significantly higher proportion of Bungaree ewes had less than 1000 sperm in the fallopian tubes 24 hours after service, but there were no significant differences in any other character examined. Contrary to an earlier report, fertilization failure is unlikely to have been a major cause of a difference in the fertility of the two strains in the two years, and embryonic loss is likely to have made a significant contribution to the difference in their fertility.


1971 ◽  
Vol 68 (2) ◽  
pp. 413-416 ◽  
Author(s):  
C. Hirschhäuser ◽  
M. Kionke ◽  
E. Daume ◽  
R. Buchholz

ABSTRACT The human genital tract contains inhibitors of trypsin in cervical mucus, uterine endometrium, uterine mucus, Fallopian tubes and their secretion products.


1991 ◽  
Vol 10 (2) ◽  
pp. 137-139 ◽  
Author(s):  
I.G. Salas Herrera ◽  
R.M. Pearson ◽  
P. Turner

1 Concentrations of albumin and alpha-1-acid glycoprotein (AGP) in human cervical mucus have been measured by a radial immunodiffusion technique. 2 The cervical mucus samples were obtained from women on combined oral contraceptives (Group A) and from women not taking this medication (Group B). In group A the mean level of albumin was 75.6 (range 22-198) mg 1-1 and for AGP 6.5 (range 3-12) mg 1-1. In group B the mean level of albumin was 72.9 (range 22-148) mg 1-1 and for AGP 6.6 (range 3-14) mg 1 -1. 3 The levels of albumin and AGP in cervical mucus were less than 1% of the concentration in serum and were not affected by combined oral contraceptives. 4 The clinical and toxicological consequences of these observations, in terms of the disposition of drugs and other chemicals in the female genital tract, await elucidation.


1963 ◽  
Vol 16 (3) ◽  
pp. 687 ◽  
Author(s):  
PE Mattner

Observations were made on the distribution of spermatozoa in the genital tract of the ewe at 15 min, and at 4, 24, and 48 hr after coitus. A total of 60 animals was used. The number of spermatozoa in t,he cervix wa.s highest at 15 min and in the uterus the maximum number was recorded at 24 hr. Stress decreased the number of spermatozoa present in the fallopian tubes at 15 min after coitus but did not influence the transport of spermatozoa into the cervix, the distribution of spermatozoa within the genital tract at 4, 24, and 48 hI', the number of spermatozoa on the zona pellucida of recovered eggs, or the proportion of eggs fertilized.


1995 ◽  
Vol 1 (5) ◽  
pp. 462-478 ◽  
Author(s):  
Waltraud Eggert-Kruse ◽  
Gerhard Rohr ◽  
Wolfram Ströck ◽  
Susanne Pohl ◽  
Beate Schwalbach ◽  
...  

Abstract The clinical significance of micro-organisms in semen samples of asymptomatic subfertile patients is a matter of constant debate. Usually little attention is paid to anaerobic bacteria as they are sensitive to transportation and culturing, and differentiation is difficult, costly and time-consuming. In the present study, special screening was carried out for anaerobes in ejaculates in addition to the routine microbial cultures of genital secretions of both partners. In addition to standard semen analysis and evaluation of sperm ability to penetrate cervical mucus (CM) in vivo (postcoital testing) and in vitro using a standardized test system, semen samples from 126 randomly chosen males of couples with a median duration of infertility of 4 years were examined for colonization with anaerobic bacteria. All couples were without clinical signs or symptoms of genital tract infection. The special care taken for anaerobic growth in semen samples gave a high rate of positive cultures and showed that nearly all ejaculates (99%) were colonized with anaerobic micro-organisms, and potentially pathogenic species were found in 71% of men. This rate was more than four times higher than that obtained with routine cultures and standard transportation (16%). Anaerobic bacterial growth of ≥106 colony forming units (CFU)/ml was seen in 42% (total range 103-108 CFU/ml). In addition, aerobic growth was found in 96%(≥106 CFU/ml in 21%), potentially pathogenic species in 61% of semen specimens. There were no marked differences in the prevalence of anaerobic micro-organisms in patients with reduced or normal sperm count, motility or morphology. Nor was there any significant difference in anaerobic colonization between samples with impaired or good ability to penetrate CM of female partners (in vivo or in vitro), or the CM of fertile donors in the in-vitro sperm-cervical mucus penetration test (SCMPT) in this asymptomatic group of patients. There was no clear association between microbial colonization and subsequent fertility in vivo within an observation period of 6 months. The results of this study suggest that anaerobic bacteria are often not detected when routine methods for microbial evaluation are used. This should be considered during assisted reproduction and in patients with symptoms of genital tract infection and should lead to further studies in infertile patients where subclinical infection or inflammation is indicated by specific markers in semen samples.


2013 ◽  
Vol 3 (5) ◽  
pp. 356-360 ◽  
Author(s):  
S Gon ◽  
A Basu ◽  
B Majumdar ◽  
TK Das ◽  
M Sengupta ◽  
...  

Background: Fallopian tubes are common surgical specimen in the pathology laboratory; still there is a lack of data to describe the frequency of various histological fi ndings. The aim and objectives of this study was to describe the various histopathological fi ndings of fallopian tubes. Materials and Methods: Two thousand fi ve hundred and seventy fi ve cases where fallopian tubes were removed either separately or along with other female genital tract organs were studied retrospectively and their histopathological fi ndings documented. Results: Ectopic pregnancy comprised maximum number of cases closely followed by salpingitis. Primary neoplastic lesions were rare as compared to secondary malignancies. Serial sections of fallopian tube and sections from representative areas are essential for a pathologist so that the diagnosis of these pathological entities is not missed. Conclusion: Though the fallopian tubes remain unremarkable in majority of the surgical pathological specimens, it must be subjected for histopathological examination to demonstrate the pathological lesions. Journal of Pathology of Nepal (2013) Vol. 3, No.1, Issue 5, 356-360 DOI: http://dx.doi.org/10.3126/jpn.v3i5.7858


2020 ◽  
Vol 13 (2) ◽  
pp. e232880
Author(s):  
Vishwajeet Singh ◽  
Gyanendra Singh ◽  
Rahul Janak Sinha ◽  
Suresh Babu

Genital tuberculosis (TB) in women is a chronic disease with low-grade symptoms. Genital tract tuberculosis is usually secondary to extragenital TB. The fallopian tubes are most commonly affected, and along with endometrial involvement, it causes infertility in such patients. Involvement of the cervix and the vulva is very rare. We present one such rare case of vulvar tuberculosis presented with a large ulcer diagnosed on histopathology and treated with antitubercular chemotherapy.


1971 ◽  
Vol 11 (51) ◽  
pp. 375 ◽  
Author(s):  
AWH Braden

One hundred five-year-old Merino ewes in medium condition (44 kg) were fed at one-third of the maintenance level from the start of joining until 3-4 weeks post-coitum (P.C.). The mean decrease in liveweight in the 3-4 weeks was 1.5 kg/week. One hundred similar ewes were fed so as to maintain liveweight. A ram was joined with each group during daylight hours for 15 days, and the course of mating observed constantly. Ewes were killed either 1-2 days p.c. or 24-38 days p.c. There was no evidence of an effect of the under-nutrition on mating behaviour, number of spermatoza in the ewe genital tract, fertilization rate (89 per cent for both) or early embryo losses (7 per cent and 3 per cent). The relatively low embryonic loss appeared to be related to the low ovulation rate (1.16).


1969 ◽  
Vol 22 (1) ◽  
pp. 181 ◽  
Author(s):  
PE Mattner ◽  
KW Entwistle ◽  
ICA Martin

The distribution of spermatozoa in the genital tract was determined in ewes killed 4 hr or 24 hr after cervical insemination with 100 million live spermatozoa from either freshly ejaculated or deep.frozen ram semen. At 4 hr, greater numbers of spermatozoa were present in the cervices, uteri, and fallopian tubes in ewes inseminated with fresh semen than in ewes inseminated with frozen semen. At 24 hr, the numbers of spermatozoa in the uteri and fallopian tubes of ewes inseminated with fresh semen had increased relative to the numbers at 4 hr but no spermatozoa were present in the uterus and fallopian tubes in ewes inseminated with frozen semen.


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