scholarly journals Passage, Survival, and Fertility of Deep-Frozen Ram Semen in the Genital Tract of the Ewe

1969 ◽  
Vol 22 (1) ◽  
pp. 181 ◽  
Author(s):  
PE Mattner ◽  
KW Entwistle ◽  
ICA Martin
Keyword(s):  
Genital Tract ◽  
Fallopian Tubes ◽  
Frozen Semen ◽  
Fresh Semen

The distribution of spermatozoa in the genital tract was determined in ewes killed 4 hr or 24 hr after cervical insemination with 100 million live spermatozoa from either freshly ejaculated or deep.frozen ram semen. At 4 hr, greater numbers of spermatozoa were present in the cervices, uteri, and fallopian tubes in ewes inseminated with fresh semen than in ewes inseminated with frozen semen. At 24 hr, the numbers of spermatozoa in the uteri and fallopian tubes of ewes inseminated with fresh semen had increased relative to the numbers at 4 hr but no spermatozoa were present in the uterus and fallopian tubes in ewes inseminated with frozen semen.

scholarly journals Effect of Oral Administration of Selenium and Vitamin E on the Quality of Fresh, Refrigerated and Frozen Semen in French Bulldog Breed Dogs

2017 ◽  
Vol 45 (1) ◽  
pp. 7
Author(s):  
Marcelo George Mungai Chacur ◽  
Mariana Grandis Ripari de Souza ◽  
Camila Dutra de Souza ◽  
Camila Pires Cremasco
Keyword(s):  
Vitamin E ◽  
Citric Acid ◽  
Oral Administration ◽  
Liquid Nitrogen ◽  
Sperm Concentration ◽  
Oral Supplementation ◽  
Frozen Semen ◽  
French Bulldog ◽  
Fresh Semen

Background: New methodologies have been developed seeking to maximize pregnancy rate in female dogs created in commercial kennels, and also in order to maintain the quality of canine semen after dilution, refrigeration or freezing. One of the main factors that generate damage to sperm is oxidative stress, to minimize sperm damage, selenium and antioxidants like vitamin E are administered, by oral administration, seeking to improve the quality of semen. The objective was to study the effect of vitamin E and selenium, by oral administration, in the quality of fresh, refrigerated and frozen semen in adult dogs French Bulldog breed.Materials, Methods & Results: Semen samples were collected from 5 adult dogs, French Bulldog breed, being 2 semen drawing before the daily oral supplementation with vitamin E and selenium (ESE®) and semen drawing at 20, 40 and 60 days after the beginning of oral supplement. The ejaculated samples were diluted in TRIS - fructose citric acid (3.28 g TRIS-hydroxy-methyl-amino-methane, 1.78 g of citric acid monohydrate and 1.25 g of D - fructose, dissolved in 100 mL of distilled water and added of 20% egg yolk and 6% of glycerol. The characteristics evaluated in fresh semen were: volume (mL), color, appearance, concentration (x106 / mL), sperm motility (%), sperm strength (1 to 5) and morphology (%). For refrigerated and frozen semen were analyzed: sperm motility (%), sperm strength (1-5) and morphology (%). Diluted semen samples were centrifuged at: 1500 g/10 min and “pellets” formed by sperm of each ejaculated, detached from the tube wall were diluted homogeneously in the diluent TRIS type up to the final volume of 1.5 mL. After that, packaged in 0.5 mL French straws, kept under refrigeration at 5ºC/4 h, placed in nitrogen vapor at -120ºC/15 min, and dipped in liquid nitrogen at -196ºC and then stored on identified rachis and stored in liquid nitrogen container until the time of thawing in  water bath at 37°C/30 s for semen microscopic analysis. Data from fresh, refrigerated and frozen semen were statistically analyzed by analysis of variance and the average compared by 5% of Tukey test. Fresh semen sperm concentration differed (P < 0.05) between the samples, rising after 40 days after the beginning of oral supplementation with selenium and vitamin E. For the spermatic strength, better score (P < 0.05) was observed at collection 4, in 40 days after the beginning of oral supplementation to dogs. For fresh and refrigerated semen, the total defects, defects of head, acrosome and tail did not differ (P > 0.05) between the samples. Total sperm defects and minor head and tail defects did not differ (P > 0.05) between the samples in post-thawing. Regarding the acrosome defects after thawing, there was a significant reduction (P < 0.05) in samples performed 40 and 60 days after the beginning of oral supplementation with selenium and vitamin E.Discussion: Attention should be paid for what purpose the extenders within the refrigeration or freezing biotech will be used. The managed supplement, by oral administration, containing selenium and vitamin E, influenced beneficially raising the sperm concentration in fresh semen and decreasing the acrosome defects in frozen semen. Oral administration of supplementation with selenium and vitamin E is recommended for improving the quality of fresh and frozen semen in dogs.

scholarly journals Evaluating the ability of semen cryopreservation of Blance Blue Belge bull cattle in Vietnam

2021 ◽  
Vol 19 (2) ◽  
pp. 237-244
Author(s):  
Nguyen Huu Duc ◽  
Pham Thu Giang ◽  
Tran Thi Binh Nguyen ◽  
Nguyen Thi Mai ◽  
Bui Dai Phong
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Joint Stock Company ◽  
Stock Company ◽  
Cattle Breeding ◽  
Semen Cryopreservation ◽  
Semen Collection ◽  
Frozen Semen ◽  
Semen Volume ◽  
Fresh Semen

The objective of this study was to determine the semen cryopreservation capacity of BBB bulls in Hanoi-Vietnam. Research conducted on the fresh semen collected from 05 BBB bulls. Results showed that semen color was normal (milky white, ivory white, ivory yellow), semen volume ranged from 6.35 mL to 7.48 mL (P <0.05), initial motility of semen ranged from 80.53% to 82.92% (P <0.05), sperm concentration in semen  ranged from 1.02 x 109 sperms/ml to 1.12 x 109 sperms/mL (P <0.05), abnormal sperm ratio ranged from 6.45% to 8.12% (P <0.05), alive sperm ratio ranged from 76.34% to 82.97% (P <0.05), sperm motility after thawing from straw semen ranged from 71.33% to 75.92% (P<0.05). In conclusion, successfully semen collection from 05 breeding BBB bulls at Hanoi Cattle Breeding Joint Stock Company, semen samples had normal color and good quantity and quality, suitable for production of frozen semen; and semen cryopreservation of straws of the 05 bull BBB semen mentioned at -196oC, sperm motility after freezing-thawing reached the economic and technical norms of 675/2014 of the Ministry of Agriculture and Rural Development.

scholarly journals Viability of Peranakan Etawah Liquid Semen Preserved in Tris Substituted with Various Energy Sources

2020 ◽  
Vol 25 (2) ◽  
pp. 68
Author(s):  
Nurul Afzan Hilda Zakiya ◽  
A H Yanti ◽  
T R Setyawati
Keyword(s):  
Energy Source ◽  
Artificial Insemination ◽  
Block Design ◽  
Egg Yolk ◽  
Energy Sources ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Semen Preservation ◽  
Semen Extender ◽  
Fresh Semen

The use of liquid semen for artificial insemination program of Etawah crossbreed goat (PE) is an alternative to replace frozen semen which is constrained by limited and expensive facilities. Production of liquid semen is faster than frozen semen, but the viability of liquid semen which preserved with a standard extender such as tris egg yolk is very short. The purpose of this study was to determine the viability of PE goat semen in egg yolk tris substituted with energy sources such as glucose, galactose, and mannose and to determine the most efficient energy source for semen preservation. This research was conducted from August to September 2018 at the Artificial Insemination Center in Lembang, West Java. This study was designed in a randomized block design (RBD) consist of three experimental groups divided into five groups. Fresh semen of PE goats were preserved using extender which energy source has been modified. Results showed that using glucose in PE goat semen extender produced the best motility among other groups (64.29 ± 9.2%). The highest viability was found in extender with fructose substitution (86.76 ± 2.3%). The longest viability of liquid semen was found in the extender with glucose substitution. It lasted for six days.

scholarly journals Repeatability and Phenotypic Correlation Among Semen Quality Traits in Holstein Bulls

2018 ◽  
Vol 42 (4) ◽  
Author(s):  
Argi Argiris ◽  
Siswanto Imam Santoso ◽  
Yon Supri Ondho ◽  
Edy Kurnianto
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Intraclass Correlation ◽  
Correlation Method ◽  
Phenotypic Correlation ◽  
Quality Traits ◽  
Frozen Semen ◽  
Holstein Bulls ◽  
Fresh Semen ◽  
Selection Of

The purpose of this research was to analysis the value of repeatability and correlation among the traits affecting the production of frozen semen from Holstein’s bull in Indonesia. Repeatability and correlation were calculated based on the data of frozen semen production of 15.699 records from 44 Holstein bulls at Singosari Artificial Insemination Center (SAIC) and 8.935 records from 39 Holstein bulls at Lembang Artificial Insemination Center (LAIC). Repeatability for volume, motility, fresh semen concentration and frozen semen production was evaluated by intraclass correlation method. The repeatability values of LAIC for volume, motility, fresh semen concentration and frozen semen production were 0.60; 0.54; 0.37 and 0.47. The repeatability values of SAIC for volume, motility, fresh semen concentration and frozen semen production were 0.54; 0.30; 0.43 and 0.29. The linear correlation value between volume, motility and fresh semen concentration with the amount of semen produced per collections were 0.41, 0.36, and 0.58. Concentration was the most factors influencing the number of frozen semen produced. The effectiveness of the selection of Holstein's frozen semen producing could be determined by the value of repeatability and the phenotypic correlation among semen quality traits such as volume, motility, concentration and frozen semen production.

scholarly journals Spermatozoa in the Genital Tract of the Ewe II. Distribution After Coitus

1963 ◽  
Vol 16 (3) ◽  
pp. 687 ◽  
Author(s):  
PE Mattner
Keyword(s):  
Zona Pellucida ◽  
Genital Tract ◽  
Fallopian Tubes

Observations were made on the distribution of spermatozoa in the genital tract of the ewe at 15 min, and at 4, 24, and 48 hr after coitus. A total of 60 animals was used. The number of spermatozoa in t,he cervix wa.s highest at 15 min and in the uterus the maximum number was recorded at 24 hr. Stress decreased the number of spermatozoa present in the fallopian tubes at 15 min after coitus but did not influence the transport of spermatozoa into the cervix, the distribution of spermatozoa within the genital tract at 4, 24, and 48 hI', the number of spermatozoa on the zona pellucida of recovered eggs, or the proportion of eggs fertilized.

scholarly journals Spectrum of histopathological lesions in the fallopian tubes

2013 ◽  
Vol 3 (5) ◽  
pp. 356-360 ◽  
Author(s):  
S Gon ◽  
A Basu ◽  
B Majumdar ◽  
TK Das ◽  
M Sengupta ◽  
...  
Keyword(s):  
Genital Tract ◽  
Histopathological Examination ◽  
Female Genital Tract ◽  
Secondary Malignancies ◽  
Fallopian Tubes ◽  
Serial Sections ◽  
Pathology Laboratory ◽  
Surgical Specimen ◽  
Neoplastic Lesions ◽  
Female Genital

Background: Fallopian tubes are common surgical specimen in the pathology laboratory; still there is a lack of data to describe the frequency of various histological fi ndings. The aim and objectives of this study was to describe the various histopathological fi ndings of fallopian tubes. Materials and Methods: Two thousand fi ve hundred and seventy fi ve cases where fallopian tubes were removed either separately or along with other female genital tract organs were studied retrospectively and their histopathological fi ndings documented. Results: Ectopic pregnancy comprised maximum number of cases closely followed by salpingitis. Primary neoplastic lesions were rare as compared to secondary malignancies. Serial sections of fallopian tube and sections from representative areas are essential for a pathologist so that the diagnosis of these pathological entities is not missed. Conclusion: Though the fallopian tubes remain unremarkable in majority of the surgical pathological specimens, it must be subjected for histopathological examination to demonstrate the pathological lesions. Journal of Pathology of Nepal (2013) Vol. 3, No.1, Issue 5, 356-360 DOI: http://dx.doi.org/10.3126/jpn.v3i5.7858

scholarly journals Vulvar tuberculosis

2020 ◽  
Vol 13 (2) ◽  
pp. e232880
Author(s):  
Vishwajeet Singh ◽  
Gyanendra Singh ◽  
Rahul Janak Sinha ◽  
Suresh Babu
Keyword(s):  
Chronic Disease ◽  
Genital Tract ◽  
Rare Case ◽  
Low Grade ◽  
Fallopian Tubes ◽  
Genital Tuberculosis ◽  
Large Ulcer

Genital tuberculosis (TB) in women is a chronic disease with low-grade symptoms. Genital tract tuberculosis is usually secondary to extragenital TB. The fallopian tubes are most commonly affected, and along with endometrial involvement, it causes infertility in such patients. Involvement of the cervix and the vulva is very rare. We present one such rare case of vulvar tuberculosis presented with a large ulcer diagnosed on histopathology and treated with antitubercular chemotherapy.

9 ENDOSCOPY-MEDIATED INTRATUBAL INSEMINATION IN THE COW – A PRELIMINARY REPORT ABOUT THE APPLICATION OF A NOVEL MINIMALLY INVASIVE INSEMINATION TECHNIQUE

2016 ◽  
Vol 28 (2) ◽  
pp. 134
Author(s):  
K. Stein ◽  
V. Havlicek ◽  
S. Papp ◽  
F. Palm ◽  
G. Brem ◽  
...  
Keyword(s):  
Reproductive Tract ◽  
Capillary Tube ◽  
Vaginal Wall ◽  
Uterine Horn ◽  
Female Reproductive Tract ◽  
Glass Capillary ◽  
Cell Stage ◽  
Frozen Semen ◽  
Hoechst Dye ◽  
Fresh Semen

On their long path through the female reproductive tract to the fertilization site, spermatozoa are exposed to diverse influences and hazards of the cervical, uterine, and oviducal environment that naturally select viable sperms for the following fertilization. Consequently, this results in a reduction from several billions of sperms in the ejaculate to a functional sperm reservoir within the range of 102 in the isthmus of the Fallopian tube. A technique to deposit spermatozoa directly into the ampulla, thus bypassing most of the reproductive tract, enables a rigorous reduction in number of sperms deposited. Furthermore, it provides a direct assessment of sperm fertility. The aim of our study was to establish an endoscopy-assisted intratubal insemination technique using different sperm dosages, fresh or cryopreserved, to determine adequate conditions for optimal fertilization. Eighteen Simmental heifers were inseminated with fresh semen, and 9 heifers were inseminated with frozen semen using this novel technique. The heifers were synchronized using a modified Ovsynch protocol, and insemination was conducted 18 to 20 h after the second gonadotropin-releasing hormone application. Insemination of heifers was performed under epidural anaesthesia. A tubing system bearing the endoscope and an insemination device was introduced through the vaginal wall into the peritoneal cavity. The insemination device consisted of a tube connected to a curved glass capillary tube loaded with semen. After a visual examination of the ovaries for the presence of an ovulatory Graafian follicle, the capillary tube was inserted directly via the infundibulum into the ipsilateral ampulla and the semen dose was deposited. The entire procedure took ~10 min. Two days later the oviduct was flushed by the same technique. A tubing system connected to a metal catheter served for flushing the embryos and unfertilized oocytes from the oviduct into the uterine horn. Afterward, embryos and oocytes were collected by flushing the uterine horn using an embryo flushing catheter and an embryo filter (EmCon). Embryos were stained using a Hoechst dye to visualise the numbers of attached spermatozoa to the zonae pellucidae. From 18 inseminations with fresh semen doses of 7 to 28 million sperms, 7 embryos at the 2- to 8-cell stage were found. Two of these embryos had more than 10 accessory sperms (AS), 3 had 3 to 6 AS, and 2 were without AS. From 9 inseminations with frozen semen doses containing 1.5 million sperms, we obtained 2 embryos, one at the 4-cell stage without AS and one at the 8-cell stage with 5 AS. Additionally, 3 unfertilized oocytes were collected. In conclusion, these preliminary results demonstrate a promising technique for intratubal AI, which has to be further optimized by studying numbers and treatment of spermatozoa and time of insemination.

A COMPARISON OF FRESH AND FROZEN SEMEN IN THE INSEMINATION OF CONFINED SHEEP

1979 ◽  
Vol 59 (4) ◽  
pp. 685-691 ◽  
Author(s):  
G. A. LANGFORD ◽  
G. J. MARCUS ◽  
A. J. HACKETT ◽  
L. AINSWORTH ◽  
H. F. PETERS ◽  
...  
Keyword(s):  
Litter Size ◽  
Reproductive Performance ◽  
Artificial Insemination ◽  
Embryonic Mortality ◽  
Frozen Semen ◽  
Serum Gonadotropin ◽  
Early Embryonic Mortality ◽  
The Difference ◽  
Natural Service ◽  
Fresh Semen

The reproductive performance of crossbred sheep maintained in total confinement was compared after artificial insemination with fresh or frozen semen. Estrus was synchronized with progestagen-impregnated vaginal sponges and pregnant mares’ serum gonadotropin. Inseminations were performed 54 and 60 h after sponge removal. The fertility of ewes inseminated with fresh semen was significantly higher than of ewes inseminated with frozen semen. Conception rates, lambing rates and litter size were 83%, 78% and 2.2 using fresh semen and 65%, 43% and 1.8 using frozen semen. In a group of similar ewes bred by natural service, the lambing performance was comparable to that obtained with fresh semen. The difference between conception and lambing rates suggests an increase in early embryonic mortality when breeding with frozen semen and confirms the need for improved frozen semen technology.

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