scholarly journals The Effect of Auxins on the Binding of Pectin Methylesterase to Cell Wall Preparations

1957 ◽  
Vol 10 (4) ◽  
pp. 426 ◽  
Author(s):  
KT Glasziou
Keyword(s):  
Cell Wall ◽  
Acetic Acid ◽  
Jerusalem Artichoke ◽  
Pectin Methylesterase ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Indolylacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

It is shown that the plant auxins 3�indolylacetic acid, 2,4-dichlorophenoxyacetic acid, and a�naphthalene acetic acid are effective in binding pectin methylesterase (PME) to cell wall preparations from tobacco pith and tubers of the Jerusalem artichoke.

An improved culture medium for growing the orchid Cypripedium reginae axenically

1982 ◽  
Vol 60 (12) ◽  
pp. 2547-2555 ◽  
Author(s):  
Gaëtan Harvais
Keyword(s):  
Acetic Acid ◽  
Membrane Filtration ◽  
Ammonium Citrate ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Growth And Survival ◽  
Murashige Skoog ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Germination And Growth

A new medium for growing Cypripedium reginae Walt. axenically from seed was designed. Liquid culture proved unsuitable, hence a 1% agar medium supplemented with 5% potato extract was used to investigate optimal mineral element, vitamin, amino acid, sugar, and growth regulator supplements for germination, and subsequent growth. A modified Pfeffer solution with 1400 mg/L NH4NO3 + 19 mg/L ammonium citrate + 2% dextrose + 10 mg/L niacin + 5 mg/L calcium pantothenate + 5 mg/L thiamine HCl + 1 mg/L kinetin + 0.1 mg/L α-naphthaleneacetic acid gave best germination and growth to 2 years with little or no phenolic production. Gamborg's B5 medium and Murashige–Skoog (MS) medium were less than optimal when tested against the above medium. Growth regulators were more active when sterilized by membrane filtration instead of autoclaving. Of the three aminopurines tested, kinetin, benzylaminopurine (BAP), and 6(γ,γ-dimethylallylamino) purine (γγ), the order of activity was initially γγ → BAP → kinetin, but kinetin produced better greening of protocorms and plantlets, and eventually greater survival. Hence, it was chosen for further study. The auxins indole-3-acetic acid (IAA), naphthalene acetic acid (NAA), indole-3-butyric acid (IBA), and 2,4-dichlorophenoxyacetic acid (2,4-D) were also tested alone and in combination with the aminopurines. They did not stimulate germination, but improved growth and survival when combined with aminopurines. The most active of the auxins were NAA → IAA → IBA → 2,4-D. A kinetin:NAA ratio of 10:1 was very satisfactory.

scholarly journals CALLUS INDUCTION ON BANANA FLOWER’S EXPLANT IN VITRO USING 2,4-DICHLOROPHENOXYACETIC (2,4-D)

2018 ◽  
Vol 22 (2) ◽  
pp. 66
Author(s):  
RINDANG - DWIYANI ◽  
HESTIN - YUSWATI ◽  
UTAMI -
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Regeneration Medium ◽  
Indirect Organogenesis ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Banana Cultivar

ABSTRACT  The objective of the study was to obtain the best 2,4-D concentration on callus induction of the banana flowers in banana propagation using indirect organogenesis method. Kesuna, local banana cultivar obtained from Sembung Gede, Tabanan was used as explant material. Callus induction was performed using 2,4-Dichlorophenoxyacetic acid with concentration of 0; 0.5; 1.0; 1.5 and 2.0 ppm. Each treatment was represented by 3 bottles and each bottle was planted with 3 explants, so each treatment was represented by 9 explants of banana flowers. The results showed that the concentration of 2.0 ppm 2.4-D induced callus with the fastest time and gave the highest percentage of the explants producing callus. The calluses were subsequently subcultured into regeneration medium using 0.5 mg/L Benzylaminopurine (BAP) and 0.005 mg/L Napthaleneaceticacid (NAA). The calluses were subsequently sub-cultured into a regeneration medium using 0.5 ppm (BAP) and 0.005 ppm Naphthalene acetic acid (NAA) to induce shoots and roots and performed plantlets.   Keywords: 2,4-Dichlorophenoxyacetic acid, banana’s flowers, callus

scholarly journals In vitro plant regeneration of Zenia insignis Chun

2018 ◽  
Vol 13 (1) ◽  
pp. 34-41
Author(s):  
Zhou Yu-qing ◽  
Zhang Meng-jie ◽  
Zhang Deng ◽  
Zhang Jun-jie ◽  
Li Jing-jian ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Basal Medium ◽  
Deciduous Tree ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Root Induction ◽  
In Vitro Plant Regeneration ◽  
Morphogenic Callus ◽  
2,4 Dichlorophenoxyacetic Acid

AbstractZenia insignis Chun is a large, fast-growing deciduous tree. In this study, we successfully developed a reliable and efficient protocol for the regeneration of fertile plants via callus induction from leaf segments of young Z. insignis seedlings. The best results were obtained with a medium containing 11.00 μM 6-benzyladenine (6-BA), 1.20 μM indole-3-butytric acid (IBA), and 0.45 μM 2,4-dichlorophenoxyacetic acid (2,4-D), which yielded morphogenic callus within 2 weeks at a frequency of 62.23%. We tested the effect of IBA alone and in combination with 6-BA on the bud differentiation response of Z. insignis callus. Shoots differentiated normally when cultured on differentiation medium containing 6.00 μM 6-BA and 1.20 μM IBA. Regenerated buds elongated successfully in medium containing 1.20 μM gibberellic acid (GA3). The elongated shoots were then transferred to Murashige and Skoog basal medium supplemented with various combinations of naphthalene acetic acid (NAA) for root induction; well-developed roots were achieved on MS basal medium supplemented with 0.01 μM NAA at a rooting rate of 89.23%. Rooted plantlets were successfully acclimatised to a greenhouse at a survival rate exceeding 90.00%.

scholarly journals The Effect of Auxins on the Binding of Pectin Methylesterase to Cell Walls

1958 ◽  
Vol 11 (2) ◽  
pp. 127 ◽  
Author(s):  
KT Glasziou ◽  
Sue D Inglis
Keyword(s):  
Cell Wall ◽  
Calcium Ions ◽  
Cell Walls ◽  
Pectin Methylesterase ◽  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

Further studies on the binding of pectin methylesterase (PME) to cell wall preparations are described. The PME of extracts of wall preparations from artichoke tubers was separated into three fractions, A, B, and O. Two similar fractions (A and 0) were obtained from tobacco pith wall preparations. The amount of fraction A type PME which could be adsorbed to wall preparations was increased by the addition of 2,4.dichlorophenoxyacetic acid (2,4.D), but not by calcium ions. Neither 2,4.D nor calcium increased the adsorption of fraction B, but calcium and not 2,4�D increased the amount of fraction 0 adsorbed to the wall preparations.

Polarity of regeneration in excised leaves of Crassula argentea. I. A role of auxin

1983 ◽  
Vol 61 (4) ◽  
pp. 1058-1063 ◽  
Author(s):  
Karol E. Paterson
Keyword(s):  
Acetic Acid ◽  
Wound Response ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Developmental Sequence ◽  
Agar Culture ◽  
High Concentrations ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Indole 3 Acetic Acid

High concentrations of four auxins, naphthalene acetic acid, indole 3-acetic acid, 2,4-dichlorophenoxyacetic acid, and indole 3-butyric acid, added to leaf segments of Crassula argentea in agar culture eliminate the strong polarity of the regeneration by inducing the formation of distal plantlets. The auxins also changed the normal wound response by inducing the formation of callus on the cut surfaces. All of the auxins increased the numbers of roots formed and inhibited the number of shoots. Unlike cytokinin, which had no effect on the polarity but altered the developmental sequence of regeneration, none of the auxins had any effect on the normal developmental sequence of regeneration, but did affect the morphology of the newly formed roots. There were differential concentration responses for each of the four auxins.

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