Effects of cereal grains on fibre digestion in vitro

1995 ◽  
Vol 46 (2) ◽  
pp. 403 ◽  
Author(s):  
Y Opatpatanakit ◽  
RC Kellaway ◽  
IJ Lean ◽  
G Annison ◽  
A Kirby
Keyword(s):  
Rumen Fluid ◽  
Synergistic Effects ◽  
Cereal Grains ◽  
Inhibitory Effects ◽  
Cereal Grain

Cereal grains including wheat, barley, oats, maize and sorghum were incubated with strained rumen fluid in vitro with either ryegrass or lucerne in the ratios of 0:100, 25:75, 50:50, 75:25 and 100:0 at 3g�C for 24 h. Expected NDF digestibilities in mixtures of cereal grain and forage were calculated by interpolation between NDF digestibilities of each grain and of each forage. These were compared with measured NDF digestibilities to detect deviations due to synergism or inhibition. Wheat, barley and maize had inhibitory effects, whereas oats and sorghum had synergistic effects, on NDF digestibility with both forages.

scholarly journals In vitro ruminal degradability of cereal grain starch

2011 ◽  
Vol 49 (No. 4) ◽  
pp. 151-155 ◽  
Author(s):  
O. Tománková ◽  
P. Homolka
Keyword(s):  
Sodium Hydroxide ◽  
Cereal Grains ◽  
In Vitro Method ◽  
Cereal Grain ◽  
Significant Difference ◽  
Grain Starch ◽  
Ruminal Degradability ◽  
Ground Wheat ◽  
Feed Samples

An in vitro method was used to determine ruminal degradability of starch (IVRDS) in a set of cereal grains. The set included 9 feed samples, including 2 samples of ground wheat, 2 samples of wheat treated with sodium hydroxide, ground barley, barley treated with sodium hydroxide, 2 samples of ground oats and ground maize. Ruminal degradability of starch was assayed by the feed fermentation for 2, 4, 6, 16 and 24 hours. A significant difference in starch degradability was found between treated and untreated ground samples after 2-hour fermentation (13.73 ± 3.12 vs. 32.77 ± 8.17; P < 0.001), 4-hour fermentation (33.44 ± 7.31 vs. 60.30 ± 16.71; P < 0.001) and 6-hour fermentation (42.63 ± 7.13 vs. 74.20 ± 6.38; P < 0.001). On the basis of the rate of ruminal degradability of starch the order of cereal grains was as follows (from the highest to the lowest value): ground oats, ground wheat, ground barley, ground maize, wheat and barley treated with sodium hydroxide.

Effects of heavy metals and other trace elements on the fermentative activity of the rumen microflora and growth of functionally important rumen bacteria

1978 ◽  
Vol 24 (3) ◽  
pp. 298-306 ◽  
Author(s):  
C. W. Forsberg
Keyword(s):  
Trace Elements ◽  
Rumen Fluid ◽  
Inhibitory Effect ◽  
Rumen Bacteria ◽  
Inhibitory Effects ◽  
Butyrivibrio Fibrisolvens ◽  
Fermentative Activity ◽  
Rumen Microflora ◽  
High Concentrations

The inhibitory effects of high concentrations of essential and non-essential trace elements were tested on the rumen microflora using the rate of fermentation in vitro as the assay. The elements (and the concentration causing 50% inhibition) in decreasing order of toxicity were Hg2+ (20 μg/ml), Cu2+ (21 μg/ml), Cr6+ (70 μg/ml), Se4+ (73 μg/ml), Ni2+ (160 μg/ml), Cd2+ (175 μg/ml), As3+ (304 μg/ml), and As5+ (1610 μg/ml). The elements tested that were either weak or non-inhibitory at concentrations greater than 400 μg/ml included Zn2+, Cr2+, Fe2+, Mn2+, Pb2+, and Co2+. Methylmercury was as inhibitory as mercuric chloride to the fermentation. When the inhibitory effect of Cd2+ was tested on separated bacterial and protozoal fractions, it was more inhibitory to the bacteria. The inhibitory effects of trace elements were also determined for a number of axenic cultures of rumen bacteria. The bacteria which most frequently exhibited the greatest sensitivity were Bacteroides succinogenes, Ruminococcus albus, Bacteroides amytophilus, and Eubacterium ruminantium. Those often exhibiting intermediate sensitivities included Butyrivibrio fibrisolvens, Selenomonas niminantium, and Megasphera elsdenii, while Streptococcus bovis was very refractory to all elements tested. Rumen fluid provided a modest protective effect for the bacteria.

scholarly journals Dual Blockade of Lactate/GPR81 and PD-1/PD-L1 Pathways Enhances the Anti-Tumor Effects of Metformin

Biomolecules ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1373
Author(s):  
Shaomeng Chen ◽  
Xiuman Zhou ◽  
Xin Yang ◽  
Wanqiong Li ◽  
Shuzhen Li ◽  
...  
Keyword(s):  
Tumor Regression ◽  
Synergistic Effects ◽  
Serum Lactate ◽  
Jurkat Cells ◽  
Future Application ◽  
Inhibitory Effects ◽  
Dual Blockade ◽  
Lactate Levels

Metformin is a widely used antidiabetic drug for cancer prevention and treatment. However, the overproduction of lactic acid and its inefficiency in cancer therapy limit its application. Here, we demonstrate the synergistic effects of the lactate/GPR81 blockade (3-hydroxy-butyrate, 3-OBA) and metformin on inhibiting cancer cells growth in vitro. Simultaneously, this combination could inhibit glycolysis and OXPHOS metabolism, as well as inhibiting tumor growth and reducing serum lactate levels in tumor-bearing mice. Interestingly, we observed that this combination could enhance the functions of Jurkat cells in vitro and CD8+ T cells in vivo. In addition, considering that 3-OBA could recover the inhibitory effects of metformin on PD-1 expression, we further determined the dual blockade effects of PD-1/PD-L1 and lactate/GPR81 on the antitumor activity of metformin. Our results suggested that this dual blockade strategy could remarkably enhance the anti-tumor effects of metformin, or even lead to tumor regression. In conclusion, our study has proposed a novel and robust strategy for a future application of metformin in cancer treatment.

scholarly journals Hairless Canaryseed: A Novel Cereal with Health Promoting Potential

Nutrients ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 1327 ◽  
Author(s):  
Emily Mason ◽  
Lamia L’Hocine ◽  
Allaoua Achouri ◽  
Salwa Karboune
Keyword(s):  
Unsaturated Fatty Acids ◽  
The United States ◽  
Cereal Grains ◽  
Antihypertensive Activity ◽  
Human Consumption ◽  
Silica Fibers ◽  
Cereal Grain ◽  
Health Promoting ◽  
Immense Potential

Glabrous canaryseeds were recently approved for human consumption as a novel cereal grain in Canada and the United States. Previously, canaryseeds were exclusively used as birdseed due to the presence of carcinogenic silica fibers; therefore the nutritional value of the seeds has been seriously overlooked. Two cultivars of glabrous canaryseeds (yellow and brown) were created from the hairy varieties. They are high in protein compared to other cereal grains, and contain high amounts of tryptophan, an amino acid normally lacking in cereals, and are gluten-free. Bioactive peptides of canaryseeds produced by in vitro gastrointestinal digestion have shown antioxidant, antidiabetic, and antihypertensive activity. The seeds contain other constituents with health promoting effects, including unsaturated fatty acids, minerals, and phytochemicals. Anti-nutritional components in the seeds are comparable to other cereal grains. Because of their beneficial health effects, canaryseeds should be regarded as a healthy food and have immense potential as a functional food and ingredient. Further research is required to determine additional bioactive peptide activity and capacity, as well as differences between the yellow and brown cultivars.

scholarly journals Inhibitory effects of sulphur compounds, copper and tungsten on nitrate reduction by mixed rumen micro-organisms

1989 ◽  
Vol 61 (3) ◽  
pp. 741-748 ◽  
Author(s):  
Junichi Takahashi ◽  
Nobuyuki Johchi ◽  
Hiroshi Fujita
Keyword(s):  
Nitrate Reduction ◽  
Rumen Fluid ◽  
Sodium Sulphate ◽  
Microbial Reduction ◽  
Inhibitory Effects ◽  
Sulphur Compounds ◽  
The Media ◽  
Micro Organisms ◽  
And Tungsten

1. The inhibitory effects of inorganic and organic sulphur-containing compounds, copper and tungsten on nitrate reduction by mixed rumen micro-organisms were investigated in two in vitro studies.2. Coarsely strained rumen fluid from nitrate-adapted (Expt 1) or non-adapted (Expt 2) Suffolk Down wethers maintained on lucerne (Medicago sativa) cubes was used as an inoculum. In Expt 1, anaerobic incubation was carried out for 24 h for each medium supplemented with 10 mM-sodium nitrate and the following chemicals: 0, 1, 2, 3, 5, 8 and 10 mM-sodium sulphide, 1 and 10 mM-sodium sulphite, 1 and 10 mM-sodium sulphate, 1 and 10 mM-L-cysteine, 1 and 10 mM-DL-methionine, 1 mM-sodium tungstate and I mM-copper sulphate. In Expt 2, 1 and 10 mM-Na2S, 1 and 10 mM-L-cysteine, 1 mM-Na2WO4, and 1 mM-CuSO4were added to incubation media to test for chemical inhibition of microbial reduction of nitrate.3. In Expt 1, the amount of nitrite formed decreased with increasing concentration of sulphide-S added. The additions of L-cysteine, W and Cu suppressed nitrite formation in media from both nitrate-adapted and non-adapted sheep.4. In contrast to the effects of sulphide, L-cysteine and W counteracted, to some degree, nitrate-induced reduction of volatile fatty acid (VFA) production. Addition of Cu to the media resulted in a further depression of VFA production.

Protein supplementation of cows grazing limited amounts of paspalum (Paspalum dilatatum Poir.)-dominant irrigated pasture in mid-lactation

2000 ◽  
Vol 40 (7) ◽  
pp. 923 ◽  
Author(s):  
W. J. Wales ◽  
D. W. Dellow ◽  
P. T. Doyle
Keyword(s):  
Milk Production ◽  
Cottonseed Meal ◽  
Milk Fat ◽  
Rumen Fluid ◽  
Protein Supplementation ◽  
Canola Meal ◽  
Paspalum Dilatatum ◽  
Protein Supplements ◽  
Cereal Grain

Two experiments were undertaken in summer to investigate production responses by dairy cows offered paspalum (Paspalum dilatatum Poir.)-dominant irrigated pasture to the inclusion of protein supplements with different rumen degradable protein characteristics in cereal grain-based concentrates. In experiment 1, cows in mid lactation were offered limited amounts of herbage, at an allowance of 28 kg DM/cow. day together with 8.0 kg DM/cow. day of a pelleted supplement containing: (i) mainly barley and wheat; (ii) barley, wheat and canola meal; or (iii) barley, wheat and cottonseed meal for 35 days. The 3 supplements were formulated to have similar in vitro DM digestibilities. In experiment 2 aspects of rumen function were measured in 9 rumen fistulated lactating cows, individually fed indoors on paspalum-dominant herbage harvested daily with a finger bar mower. Cows were offered the same amount of supplements for 28 days. Pasture intakes were similar to those of the grazing cows in experiment 1. In experiment 1, there were no effects (P>0.05) of dietary treatment on herbage intake (10.2 kg DM/cow. day) or selection of nutrients from herbage. The concentration of crude protein in the total diet consumed was lower (P<0.05) for the barley + wheat treatment (137 g/kg DM), compared with the barley + wheat + canola meal and barley + wheat + cottonseed meal treatments, which were 179 and 183 g/kg DM, respectively. In vitro DM digestibility of the feed consumed by cows was similar across the treatments, and all diets contained greater than 400 g neutral detergent fibre/kg DM, indicating there were no limitations due to dietary fibre. There were no significant effects (P>0.05) of dietary treatments on milk production (21.8 kg/cow. day), milk fat concentration (34.1 g/kg) or milk protein concentration (29.8 g/kg). Urea-N was higher (P<0.01) in the milk of cows fed the barley + wheat + canola meal and the barley + wheat + cottonseed meal treatments compared with the barley + wheat treatment. In experiment 2, rumen fluid pH was higher in cows consuming barley + wheat pellets than in cows consuming barley + wheat + canola meal on most sampling occasions. Rumen ammonia-N concentration was lowest in the barley + wheat treatment and highest in the barley + wheat + canola meal treatment. When cows grazing paspalum-dominant irrigated pasture were supplemented with 8 kg DM of a cereal-based supplement, metabolisable protein was calculated to be present in sufficient quantities to support at least 22 kg milk/cow. day and, hence, was not limiting milk production. It is concluded that protein supplements offered to cows in mid-lactation, grazing irrigated pastures in summer, are unlikely to give a milk production response beyond that achieved with cereal grain.

Comparison of starch degradation in cereal grains by incubation in vitro with rumen fluid and boiling with α-amylase solution

2007 ◽  
Vol 2007 ◽  
pp. 23-23
Author(s):  
C. M. Guedes ◽  
M. M. Rodrigues ◽  
A. Oliveira ◽  
A. L. Lourenço ◽  
S. R. Silva ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Dairy Cow ◽  
Rumen Fluid ◽  
New Method ◽  
Cereal Grains ◽  
Starch Degradation ◽  
Available Energy

Cereal grains are the most common sources of readily available energy for dairy cows. Several in vitro and in situ studies have measured starch degradability of cereal grains. However, most of these studies compared few samples and grain species and used only one method. Compared with protein, much less information is available on this topic. Such information would allow a more efficient use of energy and protein in dairy cow diets. The objective of this study was to determine starch degradation of 32 samples of 7 cereal grains using in vitro incubation with rumen fluid and a new method based on a boiling extraction with an enzymatic (thermo stable α-amylase) solution.

Virginiamycin to protect sheep fed wheat, barley or oats from grain poisoning under simulated drought feeding conditions

1995 ◽  
Vol 46 (2) ◽  
pp. 393 ◽  
Author(s):  
SI Godfrey ◽  
JB Rowe ◽  
GR Thorniley ◽  
MD Boyce ◽  
EJ Speijers
Keyword(s):  
Rumen Fluid ◽  
Cereal Grains ◽  
Wheat Grain ◽  
Blood Samples ◽  
Rumen Ph ◽  
Acid Accumulation ◽  
Simulated Drought ◽  
Fermentable Carbohydrates ◽  
Wheat Chaff

Lactic acidosis can occur when large amounts of cereal grains are given to sheep without adequate adaptation. Virginiamycin has been shown to reduce lactic acid accumulation in vitro and in the rumen of sheep following ingestion of readily fermentable carbohydrates. The aim of this experiment was to determine if virginiamycin would prevent acidosis in hungry sheep given wheat, barley or oat grain without any adaptation. Merino wethers, penned individually and given wheat chaff 300 g/day for 9 days, were offered 2.1 kg of wheat, barley or oat grain, with or without virginiamycin. Rumen fluid was collected before and 6 and 24 h after grain feeding. Blood samples were obtained before and 24 and 48 h after grain feeding. 24 h after grain feeding, rumen pH was lower (P0.001) and rumen L-lactate higher (P0.05) in sheep given wheat without than with virginiamycin. D- and L-lactate were produced in equal proportions in sheep given grain plus virginiamycin, while sheep fed on grain without virginiamycin produced more of the L-lactate isomer 24 h after grain feeding. There was no effect of virginiamycin on grain intake, although intake of sheep given oat grain was lower (P0.01) than intake of sheep given wheat or barley. The number of sheep withdrawn from the experiment after grain feeding was lower (P0.05) when wheat was given to sheep with virginiamycin compared with untreated wheat grain. Feeding virginiamycin with grain did not reduce grain intake, but reduced the severity of acidosis in sheep given wheat grain.

scholarly journals PSVI-13 Effects of Saccharomyces cerevisiae and Aspergillus-based enzyme extracts on rumen fermentation, fiber digestibility, and plasma metabolome of beef steers fed red clover/orchard hay

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 434-434
Author(s):  
Ibukun M Ogunade ◽  
Devan M Compart ◽  
Andres A Pech-Cervantes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Synergistic Effects ◽  
Plasma Concentrations ◽  
Red Clover ◽  
Rumen Fermentation ◽  
Beef Steers ◽  
Fiber Digestibility

Abstract Saccharomyces cerevisiae (SC) and Aspergillus-based enzyme extracts (ABE) are respectively fed to improve gut health and forage digestibility in ruminants. Also, cells of SC contain polyamines which can regulate the immune function. This study evaluated the synergistic effects of SC and ABE on rumen fermentation, plasma polyamine concentrations, and in vitro fiber digestibility of beef steers fed red clover/orchard hay. Eight rumen-cannulated Holstein steers were assigned to 4 treatments arranged in a 4 × 4 Latin square design with four 21-d periods. Dietary treatments were (1) CON (basal diet without additive), (2) SC (CON + 15 g/d of live SC; PMI, Arden Hills, MN, (3) ABE (5 g of ABE; PMI, Arden Hills, MN, USA) and (4) SC+ABE (15 g/d of live SC + 5 g of ABE). On d 21, blood samples were collected before morning feeding for analysis of plasma polyamines (spermines, spermidine, and putrescine). Rumen fluid samples were collected 3, 6, and 9 hours after feeding for analysis of VFA and in vitro dry matter and fiber digestibility. The model included the effects of treatment, period, and random effects of cow and square. Compared with CON, neither SC nor ABE had effects (P &gt; 0.10) on ruminal VFA profile. In contrast, SC+ABE increased (P ≤ 0.05) the ruminal concentrations of acetate, butyrate, valerate and total VFA concentrations. In vitro dry matter and fiber digestibilities were increased (P &lt; 0.05) by SC+ABE, but not by SC or ABE alone. Both SC and SC+ABE increased (P &lt; 0.05) plasma concentrations of spermidine and spermine. These results suggest that SC supplementation with or without ABE increased plasma concentrations of polyamines; however, SC and ABE synergistically improved the rumen fermentation and in vitro fiber and DM digestibility.

Inhibitory effects of bioaccessible anthocyanins and procyanidins from apple, red grape, cinnamon on α-amylase, α-glucosidase and lipase

2020 ◽  
pp. 1-9
Author(s):  
Pınar Ercan ◽  
Sedef Nehir El
Keyword(s):  
Inhibitory Activity ◽  
Digestive Enzymes ◽  
Positive Control ◽  
Anthocyanin Content ◽  
Inhibitory Effects ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Before And After ◽  
Red Grape

Abstract. The goals of this study were to determine and evaluate the bioaccessibility of total anthocyanin and procyanidin in apple (Amasya, Malus communis), red grape (Papazkarası, Vitis vinifera) and cinnamon (Cassia, Cinnamomum) using an in vitro static digestion system based on human gastrointestinal physiologically relevant conditions. Also, in vitro inhibitory effects of these foods on lipid (lipase) and carbohydrate digestive enzymes (α-amylase and α-glucosidase) were performed with before and after digested samples using acarbose and methylumbelliferyl oleate (4MUO) as the positive control. While the highest total anthocyanin content was found in red grape (164 ± 2.51 mg/100 g), the highest procyanidin content was found in cinnamon (6432 ± 177.31 mg/100 g) (p < 0.05). The anthocyanin bioaccessibilities were found as 10.2 ± 1%, 8.23 ± 0.64%, and 8.73 ± 0.70% in apple, red grape, and cinnamon, respectively. The procyanidin bioaccessibilities of apple, red grape, and cinnamon were found as 17.57 ± 0.71%, 14.08 ± 0.74% and 18.75 ± 1.49%, respectively. The analyzed apple, red grape and cinnamon showed the inhibitory activity against α-glucosidase (IC50 544 ± 21.94, 445 ± 15.67, 1592 ± 17.58 μg/mL, respectively), α-amylase (IC50 38.4 ± 7.26, 56.1 ± 3.60, 3.54 ± 0.86 μg/mL, respectively), and lipase (IC50 52.7 ± 2.05, 581 ± 54.14, 49.6 ± 2.72 μg/mL), respectively. According to our results apple, red grape and cinnamon have potential to inhibit of lipase, α-amylase and α-glucosidase digestive enzymes.

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