Methionine supplementation of milk proteins for preruminant lambs. II.* Effect on wool growth, liveweight gain and body composition

1975 ◽  
Vol 26 (4) ◽  
pp. 681 ◽  
Author(s):  
DM Walker

Twenty-six crossbred lambs in two equal groups, aged 2-5 days, were given a low protein diet based on cows' milk, with or without a supplement of DL-methionine, for an experimental period of 21 days. Intake of gross energy ranged from 518 to 1276 kJ/day per kg0.73 Nitrogen and sulphur retentions were estimated by balance methods and nitrogen retention was also ascertained by comparative slaughter. In the group supplemented with methionine the apparent digestibility of sulphur increased significantly, but there were no significant differences between the groups in the apparent digestibilities of dry matter and nitrogen or in gross energy. Retention of nitrogen and sulphur in the body tissues and in the wool increased significantly. There was a significant increase in wool growth and in the sulphur content of wool, but not in the nitrogen content. Methionine supplementation had no significant effect on liveweight gain. The gain in empty liveweight of the supplemented lambs comprised significantly more protein and significantly less fat than that of the unsupplemented lambs. *Part I, Aust. J. Agric. Res. 26: 673 (1975)

2008 ◽  
Vol 88 (2) ◽  
pp. 205-212 ◽  
Author(s):  
G. Lopez ◽  
S. Leeson

In commercial nutrition and in research studies, metabolizable energy (ME) is the standard measure of energy used in describing energy requirements and diets for poultry. The provision of dietary energy will influence the intake of all other nutrients. Broilers exhibit an outstanding ability to control their energy intake by adjusting their feed intake as diet energy concentration changes. There is still considerable debate on the accuracy, precision and usefulness of different procedures used for determining ME values of diets and ingredients. ME intake is generally partitioned into energy retained (ER) in body tissues (mainly as fat and protein) and as heat production (HP): ME = HP + ER. There are few reported estimates of HP and its components, fasting heat production (FHP), heat production due to physical activity and the thermic effect of feeding (TEF). Requirements for maintenance (MEm), including major components of FHP and physical activity, are established at around 155 kcal kg BW0.60. We recentlyreported that maintenance requirements for young broilers based on kg BW0.75 were 8% lower than the values estimated using kg BW0.60, and that BW raised to the exponent 0.60, was a more precise estimator. Gross energy retained in the body as fat (TERF) and protein (TERP), together contribute most of the total energy retained (TER) in the body. Efficiency of ME utilization above maintenance varies from 70 to 84% for lipid deposition in adult birds and between 37 and 85% in growing birds. Key words: Energy, broiler, metabolic rate, energy retention


1975 ◽  
Vol 26 (4) ◽  
pp. 673 ◽  
Author(s):  
DM Walker ◽  
RD Kirk

Forty-seven crossbred lambs, aged 2–5 days at the start of the experiment, were used in three experiments. In experiment 1 a supplement of DL-methionine significantly increased the nitrogen balances of lambs given low or medium protein milk replacers based on cows' milk proteins. In experiment 2 the optimum level of supplementation of a low protein diet with DL-methionine was determined. Although the nitrogen balances were significantly increased by the supplement, the actual nitrogen balances at equivalent intakes of gross energy and nitrogen were less than those in experiment 1. The low protein diets in the two experiments differed only in the source of carbohydrate: lactose in cxpcrimcnt 1; lactose + glucose in experiment 2. In the final experiment 24 lambs in a 2 x 2 factorial design were fed on low protein diets containing casein as the source of protein, and lactose or glucose as the sole source of carbohydrate, with or without a supplement of DL-methionine. There was no significant effect of the source of carbohydrate on nitrogen balance.


2017 ◽  
Vol 9 (2) ◽  
pp. 98 ◽  
Author(s):  
Afifa Khalida ◽  
Agustono Agustono ◽  
Widya Paramita

                                                                   AbstrakAsam amino adalah komponen terkecil yang menyusun protein. Sejumlah asam amino akan dihubungkan satu sama lain melalui perantara ikatan peptida untuk membentuk protein. Asam amino telah dibagi menjadi dua; yaitu asam amino esensial dan asam amino non-esensial. Asam amino esensial adalah asam amino yang tidak dapat disintesis oleh tubuh ikan; karena itu asam amino esensial harus ada dalam pakan. Lisin adalah salah satu dari sepuluh asam amino esensial, fungsi lisin adalah untuk pertumbuhan dan perbaikan jaringan tubuh. Penelitian ini bertujuan untuk mengetahui pengaruh lisin dalam pakan komersial terhadap retensi protein dan retensi energi ikan bawal air tawar. Metode yang digunakan adalah desain eksperimen rancangan acak lengkap. Perlakuan yang digunakan adalah kadar lisin yang berbeda, yaitu P0 (0%), P1 (0,6%), P2 (1,2%), P3 (2,4%), P4 (4,8%) dan perlakuan  diulang 4 kali. Parameter utama yang diamati adalah retensi protein dan retensi energi pada air tawar bawal. Parameter yang diukur didukung oleh parameter kualitas air. Analisis data menggunakan Analisis Varian (ANOVA) dan untuk menentukan perlakuan terbaik digunakan Duncan's multiple range test. Hasil penelitian menunjukkan perbedaan yang signifikan (p <0,05) dalam retensi protein dan energi adalah daging ikan bawal air tawar. Retensi protein dan retensi energi dalam pengobatan P1 (0,6% lisin), P2 (1,2% lisin), P3 (2,4% lisin) dan P4 (4,8% lisin) berbeda secara signifikan dengan perlakuan P0 (kontrol). Kualitas air selama 40 hari perlakuan ditetapkan pada suhu 27-300 C, pH 7,5-8,5, amonia 4 mg / l dan oksigen terlarut 4 mg / l.                                                                 AbstractAmino acids are the smallest components that compose proteins. A number of amino acids will be conducted to one another through the intermediary of peptide bonds to form proteins. Amino acids have divided into two; those are essential amino acids and non-essential amino acids. The essential amino acids are amino acids that can not be synthesized by the body of fish; therefore the essential amino acids must be in feed. Lysine is one of the ten essentials amino acids , the function of lysine are for the growth and repair body tissues. This research was aimed to determine the effect of lysine in commercial feed on protein retention and energy retention of freshwater Bawal.The method used is experiment with a completely randomized design as an experimental design. The treatment used is different lysine levels , namely P0 ( 0 % ) , P1 ( 0.6 % ) , P2 ( 1.2 % ) , P3 ( 2.4 % ) , P4 (4.8 % ) and treatment was repeated 4 times. The main parameters were observed protein retention and energy retention on Bawal fresh water. Parameters measured were supported by water quality parameters. Analysis of the data using Analysis of Varian ( ANOVA ) and to determine the best treatment used Duncan 's multiple range test. The results showed the significant differences ( p < 0.05 ) in protein retention and energy were freshwater pomfret fish meat. Retention of protein and energy retention in treatment P1 ( 0.6 % lysine ) , P2 ( 1.2 % lysine ) , P3 ( 2.4 % lysine ) and P4 ( 4.8 % lysine ) was significantly different with treatment P0 ( control ). The quality of water for 40 days treatment was set to temperature 27-300 C, pH 7.5-8.5, ammonia 4 mg/l and dissolved oxygen 4 mg/l.


1987 ◽  
Vol 38 (6) ◽  
pp. 1061
Author(s):  
DM Walker ◽  
SJ Al-Ali

Twenty-seven preruminant male crossbred lambs, aged 1-2 days at the start of the experiment, were used. Three lambs were sacrificed on day 1 and selected bones taken for analysis. Twenty-four lambs, in groups of three, were bottle-fed on low-phosphorus milk replacers in a 2 x 4 factorial experiment. The sole source of protein in the diets was whole beef blood, supplemented with L-isoleucine and DL-methionine, to supply either 10 or 25% of the total dietary energy as protein. At each protein level the diets were supplemented with CaCO3 to provide four ratios of Ca:P (0.5:1, 1:1, 2:1 and 10:1). The daily intake of gross energy was controlled at 880 kJ-0.73. Faeces and urine were collected separately each day and bulked during the last seven days of the experiment for the estimation of N, Ca and P. Serum Ca and P concentrations were determined at weekly intervals. The lambs were sacrificed at the end of the experimental period of 21 days and selected bones were taken for analysis. The concentrations of bone ash, Ca and P all decreased significantly when compared with values determined at the start of the experiment. The lambs given the high-protein diets showed significantly greater losses of bone ash, Ca and P, and had lower serum P, but higher serum Ca concentrations, than lambs fed on the low-protein milk replacers, irrespective of the dietary Ca:P ratios. Phosphorus excretions during the last seven days of the experiment were as follows: faecal P (day-1); low-protein group (n = 12), 3.1 � 0.5 mg kg-1; high-protein group (n = 11) , 4.1 � 0.5 mg kg-1; all lambs (n = 23), 3.6 � 0.3 mg kg-1. Urinary P (day-1): low-protein group (n = 12), 0.92 � 0.22 mg kg-1; high-protein group (n = 11) , 0.61 � 0.03 mg kg-1; all lambs (n = 23), 0.77 � 0.12 mg kg-1. Faecal and urinary P excretion was unaffected by variation in the dietary Ca:P ratio.


1975 ◽  
Vol 85 (1) ◽  
pp. 185-187 ◽  
Author(s):  
T. Fujihara ◽  
I. Tasaki

There is evidence that a large volume of saliva containing a significant quantity of urea is poured into the rumen, and also that blood urea is diffused into the rumen through the ruminal epithelium (Houpt, 1959; Weston & Hogan, 1967). Egan (1965) reported that, with sheep fed a low protein cereal hay, cellulose digestion in the rumen was increased by abomasal urea infusion. Furthermore, the utilization of recycled urea nitrogen has been investigated in sheep (McIntyre, 1971) and cattle (Vercoe, 1969) using the intraruminal and intravenous infusion techniques. The objective of the present experiment was to determine whether or not the abomasal administration of urea to adult goats fed a low quality hay would influence the nitrogen retention in the body.


1980 ◽  
Vol 20 (104) ◽  
pp. 308 ◽  
Author(s):  
BA McGregor ◽  
JW McLaughlin

The influence of the dietary concentration of protein and energy on the growth of Merino wether weaners in pens, was studied in a factorial experiment. There were two digestible energy concentrations, (12.4 and 14.2 MJ DE kg-1 DM) x five protein concentrations (9-21% CP), three replicates (each of one animal). Diets were offered ad libitum, and nitrogen and energy retention were determined by the comparative slaughter technique. Initially, the mean weight of all treatment groups was 15.5 kg liveweight (12 weeks of age), and the experiment concluded 14 weeks later. Daily dry matter intake, liveweight gain, wool growth and total body nitrogen retention increased linearly as dietary crude protein concentration increased. Higher energy concentrations reduced dry matter intake and liveweight gain, and increased wool growth. Approximately 21 % of the digestible energy intake was retained. Regressions relating carcase and non-carcase composition to compartment weight, are presented. The fastest and most efficient gain in liveweight occurred with the ration containing 20% CP, at the lower energy concentration. It is suggested that for high levels of production with Merino weaners, rations should contain at least 18% CP.


1987 ◽  
Vol 45 (2) ◽  
pp. 191-203 ◽  
Author(s):  
E. Charmley ◽  
C. Thomas

ABSTRACTA sward of perennial ryegrass was either directly ensiled at 181 g dry matter (DM) per kg fresh weight with formic acid applied at 3·6 1/t fresh material or wilted for 65 h to achieve a DM concentration of 407 g DM per kg. Physical and respiration loss were measured in the field and losses during ensiling were estimated from buried bags. The silages were offered to 58 British Friesian steers, initially 3 months of age and 101 kg live weight (LW). Ten steers were allocated to an initial slaughter group and the remainder were offered the two silages either ad libitum or at two restricted levels of feeding. These animals were slaughtered after a mean period of 112 days to estimate the increments in the components of the body.Loss of DM in the field increased from 4·3 to 86·6 g/kg on wilting and proportionately 0·75 of this additional loss was due to respiration. The fermentation characteristics of the silages were broadly similar except that the concentration of ethanol was higher in the unwilted than in the wilted silage (57·6 v. 5·6 g/kg DM). Losses of DM during ensiling were slightly higher with the unwilted (104·4 g/kg ensiled) than with the wilted (80·1 g/kg) material. Losses of nitrogen were relatively higher and energy losses lower than was the loss of DM.Wilting significantly depressed the digestibility of gross energy from 0·651 to 0·631 (P < 0·001) and at the ad libitum level steers ate proportionately 006 more wilted silage (P > 0·05) resulting in similar intakes of digestible energy. At the ad libitum level of feeding steers given wilted silage gained more LW (+55 g/day) and empty-body weight (+64 g/day) than those given unwilted silage (P < 0·05). However, steers given wilted silage contained less fat and energy in the empty body at slaughter than those given unwilted silage. Thus, wilting did not influence the efficiency of utilization of digestible energy for energy retention nor the efficiency of utilization of dietary protein for protein gain. Since efficiencies by the animal were similar and also the increased field loss on wilting was partly compensated for by a reduced silo loss, it is concluded that the proportion of energy and protein in the standing crop retained by the steers is unaffected by wilting (65 and 64 kJ/MJ and 125 and 138 g/kg N for unwilted and wilted systems respectively). Further work is needed to establish the effect of wilting for a shorter period than in the current trial by increasing the drying rate of herbage.


1971 ◽  
Vol 77 (3) ◽  
pp. 363-369 ◽  
Author(s):  
D. M. Walker ◽  
B. W. Norton

SUMMARYNineteen male cross-bred lambs, aged between 2 and 5 days, were allotted to each of three dietary treatments for an experimental period of 3 weeks. The protein contents of the diets (on a dry-matter basis) were 12·0% (diet A), 28·5% (diet B) and 45·5% (diet C). The diets were fed as artificial milks that contained 15% total solids, and the energy intakes of groups of lambs within each dietary treatment varied from below maintenance to ad lib.Metabolizable energy (ME) as a percentage of the gross energy was constant for all diets, at all levels of energy intake, at 94·3 ± 0·3%. ME intake was linearly related to energy retention above maintenance with all diets. There was a significant linear decrease in the net efficiency of ME utilization as the protein content of the diet increased. However, the individual regressions did not differ significantly from the common regression coefficient of 0·686 ± 0·018, i.e. a net efficiency of 68·6 ± 1·8%. The individual coefficients were 0·740, 0·679 and 0·634 for diets A, B and C respectively.The ME requirement for energy equilibrium (maintenance) of lambs given diet B (100·4± 11·7 kcal/kg0·73 day) was significantly lower than that of lambs given diet A (126·4 ± 14·3) or diet C (119·2 ± 15·6). The energy costs of fat and protein deposition, calculated by multiple regression analysis of the pooled data, were 11·1 kcal ME per g fat and 8·5 kcal ME per g protein respectively.


1933 ◽  
Vol 23 (1) ◽  
pp. 97-107 ◽  
Author(s):  
A. H. H. Fraser ◽  
J. A. Fraser Roberts

Summary1. Two lots of twenty sheep each were fed indoors on rations known to produce satisfactory growth. The calorific value of the two diets was the same. One lot received 52 per cent, more digestible protein than the other. The diet of the low protein group was protein deficient according to the calculations of Wood.2. The sheep were shorn at the beginning of the experiment and again at its conclusion. Fibre measurements were made on samples taken at the same two periods from the same body area. The fleece and fibres grown during the experimental period were compared in each case with the fleece and fibres grown during the pre-experimental period. The fibre measurements included fineness, length and fibre weight.


1987 ◽  
Vol 38 (6) ◽  
pp. 1061
Author(s):  
DM Walker ◽  
SJ Al-Ali

Twenty-seven preruminant male crossbred lambs, aged 1-2 days at the start of the experiment, were used. Three lambs were sacrificed on day 1 and selected bones taken for analysis. Twenty-four lambs, in groups of three, were bottle-fed on low-phosphorus milk replacers in a 2 x 4 factorial experiment. The sole source of protein in the diets was whole beef blood, supplemented with L-isoleucine and DL-methionine, to supply either 10 or 25% of the total dietary energy as protein. At each protein level the diets were supplemented with CaCO3 to provide four ratios of Ca:P (0.5:1, 1:1, 2:1 and 10:1). The daily intake of gross energy was controlled at 880 kJ-0.73. Faeces and urine were collected separately each day and bulked during the last seven days of the experiment for the estimation of N, Ca and P. Serum Ca and P concentrations were determined at weekly intervals. The lambs were sacrificed at the end of the experimental period of 21 days and selected bones were taken for analysis. The concentrations of bone ash, Ca and P all decreased significantly when compared with values determined at the start of the experiment. The lambs given the high-protein diets showed significantly greater losses of bone ash, Ca and P, and had lower serum P, but higher serum Ca concentrations, than lambs fed on the low-protein milk replacers, irrespective of the dietary Ca:P ratios. Phosphorus excretions during the last seven days of the experiment were as follows: faecal P (day-1); low-protein group (n = 12), 3.1 � 0.5 mg kg-1; high-protein group (n = 11) , 4.1 � 0.5 mg kg-1; all lambs (n = 23), 3.6 � 0.3 mg kg-1. Urinary P (day-1): low-protein group (n = 12), 0.92 � 0.22 mg kg-1; high-protein group (n = 11) , 0.61 � 0.03 mg kg-1; all lambs (n = 23), 0.77 � 0.12 mg kg-1. Faecal and urinary P excretion was unaffected by variation in the dietary Ca:P ratio.


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