Temperature influences on the growth, digestibility, and carbohydrate compositions of two tropical grasses, Panicum maximum var Trichoglume and Setaria sphacelata, and two cultivars of the temperate grass Lolium perenne

1971 ◽  
Vol 22 (4) ◽  
pp. 563 ◽  
Author(s):  
JR Wilson ◽  
CW Ford
Keyword(s):  
Lolium Perenne ◽  
Cold Water ◽  
Starch Content ◽  
Soluble Sugars ◽  
Water Soluble ◽  
Panicum Maximum ◽  
Lower Growth ◽  
Tropical Grasses ◽  
Structural Cell

Two tropical grasses, Panicunl maximum var. trichoglume and Setaria spliacelata cv. Nandi were compared with two cultivars of Lolium perenne, S.24 and Kangaroo Valley, grown in controlled environment at day/night temperatures of 15.6/10, 21 .1/15.6,26.7/21.1, and 32.2/26.7�C. The plants were harvested when still vegetative. The tropical grasses grew faster than the L. perenne cultivars at all but the lowest temperatures, and had a generally higher relative water content. The in vitro dry matter digestibility of the L. pereiine cultivars appeared inherently higher than that of the tropical grasses, but for each species digestibility Mtas modified by the environment and was highest at the lower growth temperatures. The tropical grasses were lower in alcohol- and cold water-soluble sugars, and higher in starch and structural (cell wall) carbohydrates than the L. perenne cultivars. For all grasses, as temperature increased from 15.6/10 to 26.7/21.1�C the concentration of the structural carbohydrates increased whilst that of the total non-structural carbohydrates changed relatively little. A further increase in temperature to 32.2/26.7� resulted in a marked decline in the concentration of simple sugars and structural carbohydrates whilst polymeric sugars increased. At this latter temperature the starch content in Setaria and Panicurn increased to as high as 16 and 13% of dry weight respectively. The differences in digestibility appeared to be associated with the differences in the proportions of the different carbohydrate fractions.

scholarly journals Chemical composition and in vitro ruminal degradation of hay and silage from tropical grasses

2014 ◽  
Vol 94 (4) ◽  
pp. 705-715 ◽  
Author(s):  
J. I. Arroquy ◽  
M. V. Cornacchione ◽  
D. Colombatto ◽  
C. Kunst
Keyword(s):  
Chemical Composition ◽  
Dry Matter ◽  
Gas Production ◽  
Water Soluble ◽  
Grass Species ◽  
Soluble Carbohydrate ◽  
Panicum Maximum ◽  
Tropical Grasses ◽  
Ruminal Degradation

Arroquy, J. I., Cornacchione, M. V., Colombatto, D. and Kunst, Jr., C. 2014. Chemical composition and in vitro ruminal degradation of hay and silage from tropical grasses. Can. J. Anim. Sci. 94: 705–715. This study was conducted to evaluate the effects of preservation type on chemical composition and in vitro ruminal degradation of warm-season grasses (WSG). Treatments consisted of two factors (6×2): the first factor was tropical grasses: Cenchrus ciliaris (cv. Biloela, and cv. Molopo), Chloris gayana (cv. Callide and cv. Finecut), Panicum maximum, and Brachiaria bryzanta; and the second factor was preservation type (hay vs. silage). Cell wall, hemicellulose, cellulose, and water-soluble carbohydrate (P<0.05) concentrations were different among WSG. In general, hay or silage altered fiber content compared with fresh. For instance, hemicellulose and cellulose contents were lower in silage than in hay and fresh grass (P<0.05). Gas production rates were higher in silage from 0 to 24 h of fermentation, except at 4 h of incubation. After 24 h, gas production (GP) rate was similar for both preservation types, whilst potential GP was similar between preservation types. However, silage had decreased lag time compared with hay (P<0.01). Silage had greater dry matter disappearance than hay (P<0.05), and gas production yield was similar for grass species and preservation type. Our results indicate that WSG conserved as silage showed beneficial changes in chemical composition and dry matter degradation compared with hay.

scholarly journals Formation of adaptive responses of grapes to the action of abiotic stressors of the winter period

2021 ◽  
Vol 34 ◽  
pp. 01013
Author(s):  
Natalia Nenko ◽  
Galina Kiseleva ◽  
Irina Ilina ◽  
Viktoria Sokolova ◽  
Natalia Zaporozhets
Keyword(s):  
Starch Content ◽  
Soluble Sugars ◽  
Water Soluble ◽  
Winter Period ◽  
Adaptive Responses ◽  
Adaptive Resistance ◽  
Adaptive Processes ◽  
Different Origins ◽  
Grape Varieties ◽  
Adaptive Abilities

The purpose of this work is to study the formation of adaptive reactions of grapes to the action of stressors of the winter period, by physiological and biochemical parameters, to identify varieties that have increased adaptive abilities for use in breeding. Objects of research are grape varieties of different origins: Kristall (control), Dostoynyi, Krasnostop AZOS, Vostorg, Aligote, Zarif. It was revealed that the increased resistance to winter stress conditions in the varieties Kristall, Dostoyny is achieved by a decrease in the water content of the buds by 9.09-10.40 %. In the varieties Vostorg, Dostoyny, Krasnostop AZOS adaptive resistance is achieved by increasing the starch content in the tissues of the shoots by 2.81-5.50 times in the pre-winter period. In varieties Krasnostop AZOS, Vostorg an important contribution to the formation of adaptive processes was made by water-soluble sugars, the content of which increased 2.82 and 2.89 times as a result of starch hydrolysis. An increase in the activity of peroxidase (2.49-2.75 times) indicated the instability of varieties Zarif, Aligote. Varieties Vostorg, Dostoynyi, Krasnostop AZOS have increased adaptive abilities in comparison with other studied varieties and are recommended for use in the breeding process.

scholarly journals Assessment of the Quality and Evaluation of the Antioxidant Potential of a Novel Sri Lankan Ayurvedic Polyherbal Formulation

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
H. P. Wakkumbura ◽  
W. M. D. Wickramaarachchi ◽  
L. D. A. M. Arawwawala ◽  
J. A. Liyanage ◽  
R. P. V. J. Rajapakse
Keyword(s):  
Cold Water ◽  
Antioxidant Properties ◽  
Water Soluble ◽  
Hot Water ◽  
Antioxidant Potential ◽  
In Vitro Assays ◽  
Phytochemical Screening ◽  
Anti Inflammatory ◽  
Water Extractable

Background. In Sri Lanka, a Polyherbal Ayurvedic Formulation (PHAF), which consists of powders of seven medicinal plants, is being trialed for use as an anti-inflammatory drug. In general, anti-inflammatory drugs have good antioxidant properties. Therefore, in the present study, an attempt was made to assess the quality and evaluate the antioxidant potential of PHAF. Methods. Physicochemical parameters such as ash content, extractable matter, phytochemical screening for secondary metabolites, levels of heavy metals, and microbes were determined according to standard protocols. Antioxidant activity was evaluated using five in vitro assays: total polyphenolic content (TPC), total flavonoid content (TFC), ORAC (oxygen radical absorbance capacity), DPPH (1,1-diphenyl-2-picryl-hydrazyl), and ABTS (2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt. Results. PHAF contained 5.6 ± 0.2% of moisture, 6.5 ± 0.1% of total ash, 1.4 ± 0.1% of water soluble ash, 0.9 ± 0.0% of acid insoluble ash, 7.7 ± 0.2% of hot water extractable matter, 3.9 ± 0.1% of cold water extractable matter, 10.5 ± 4.1% of hot-ethanol extractable matter, and 8.4 ± 0.2% of cold-ethanol extractable matter. Phytochemical screening revealed the presence of phenolic compounds, tannins, flavonoids, coumarins, and saponins in both aqueous and ethanolic extracts of the drug. TPC, TFC, ORAC, DPPH, and ABTS of aqueous and ethanol extracts of PHAF were 103.65 ± 4.94 and 327.07 ± 9.65 mg gallic acid equivalents/g extract, 76.6 ± 5.83 and 224.6 ± 8.42 mg quercetin equivalents/g of extract, 481.11 ± 17.30 and 1481.44 ± 30.20 mg trolox equivalents/g of extract, 79.50 ± 4.42 and 227.17 ± 6.16 mg trolox equivalents/g of extract, and 198.20 ± 4.55 and 577.08 ± 5.48 mg trolox equivalents/g of extract, respectively. Conclusion. Ethanolic extract of PHAF is better than aqueous extract in terms of antioxidant properties.

Temperature influences on the in vitro digestibility and soluble carbohydrate accumulation of tropical and temperate grasses

1973 ◽  
Vol 24 (2) ◽  
pp. 187 ◽  
Author(s):  
JR Wilson ◽  
CW Ford
Keyword(s):  
Soluble Carbohydrates ◽  
Soluble Carbohydrate ◽  
In Vitro Digestibility ◽  
Controlled Environment ◽  
Growth Stages ◽  
Panicum Maximum ◽  
Carbohydrate Accumulation ◽  
Tropical Grasses ◽  
Stage Of Development

Thirteen tropical or subtropical and 11 temperate grasses were grown in controlled environment under daylnight temperatures of 21/13, 27/19, and 32/24�C. Each plant was harvested 2 days after the fifth leaf on the main stem reached maximum length; other plants of Panicum maximum var. trichoglume and Lolium perenne cv. S.24 grown under the same conditions were also harvested 4, 8, 12, and 18 days after this stage of development. There was a consistent decline in the in vitro digestibility of most of the temperate grasses with increase in temperature from 21/13 to 32/24�C. This decline averaged about 5 digestibility units and was associated with a fall in the percentage of soluble carbohydrates. In contrast, temperature had little effect on the digestibility of most of the tropical grasses. However, in later harvests of Panicum the digestibility at 27/19 and 32/24� was consistently lower than at 21/13�. This was attributed to the more advanced growth stages at the higher temperatures. The temperate grasses accumulated much higher concentrations of soluble carbohydrate than the tropical grasses.

scholarly journals Chemical Composition and in Vitro Digestibility of Five Heavily Fertilized Tropical Grasses in Puerto Rico

1969 ◽  
Vol 59 (3) ◽  
pp. 186-198
Author(s):  
J. A. Arroyo-Aguilú ◽  
S. Tessema ◽  
R. E. McDowell ◽  
P. J. Van Soest ◽  
A. Ramírez ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Chemical Composition ◽  
Crude Protein ◽  
Pennisetum Purpureum ◽  
Grass Species ◽  
In Vitro Digestibility ◽  
Panicum Maximum ◽  
Tropical Grasses ◽  
Brachiaria Ruziziensis

Samples from five tropical grass species: Guinea (Panicum maximum), Pangola (Digitaria decumbens), Congo (Brachiaria ruziziensis), Merker (Pennisetum purpureum), and Star (Cynodon nlemfuensis), were harvested during nine weeks at ages from 7 to 63 days in southwestern Puerto Rico. The grasses were fertilized at the rate of 4480 kg/ha per year with a 15-5-10 fertilizer. The crude protein content of all grasses exceeded 10 percent up through 28 days of regrowth. Grasses declined in crude protein from a mean of 18.1 percent at 7 days to 5.6 percent at 63 days. A narrower range was observed between grasses from 42 to 63 days. The five tropical grasses possessed high contents of structural carbohydrates, principally cellulose and lignin, which increased with age, except in Pangola grass. Silica did not change uniformly with age in any of the grasses while hemicellulose was characterized by marked fluctuations. Digestibility values were lower at all stages of growth than in temperate forages of similar ages. Cellulose was negatively correlated with in vitro digestibility in all grasses except Pangola. Lignin appeared to be the predominant factor in determining digestibility. The relationship of silica to digestibility varied between species, being positive in some (Guinea and Congo) and negative in others (Star, Pangola, and Merker). The grasses may be ranked as follows with regard to their chemical composition and digestibility: Merker > Congo > Star > Guinea > Pangola. Pangola grass, though lowest in in vitro digestibility, declined least with advancing age, thus maintaining a more constant quality for a longer period of time.

Microbial activity in grass-fed in-vitro continuous cultures in response to infusion of graded levels of soluble sugars

2001 ◽  
Vol 2001 ◽  
pp. 29-29
Author(s):  
M.R.F. Lee ◽  
D.K. Leemans ◽  
D.R. Davies ◽  
R.J. Merry ◽  
J. M. Moorby ◽  
...  
Keyword(s):  
Microbial Population ◽  
Soluble Sugars ◽  
Nitrogen Supply ◽  
Water Soluble ◽  
Soluble Carbohydrate ◽  
Microbial Protein ◽  
Continuous Cultures ◽  
Water Soluble Carbohydrate ◽  
N Use

Novel lines of high sugar ryegrass have been shown to increase the efficiency of N use and milk production in zero-grazed cattle (Miller et al., 1999). An experiment was carried out to determine whether this was in part due to an increase in the efficiency of microbial protein synthesis in response to the amount and availability of water soluble carbohydrate (WSC) increasing the balance between energy and nitrogen supply to the rumen microbial population.

scholarly journals Marine Microalgae Biomolecules and Their Adhesion Capacity to Salmonella enterica sv. Typhimurium

2020 ◽  
Vol 10 (7) ◽  
pp. 2239
Author(s):  
Tatiane Winkler Marques Machado ◽  
Jenifer Mota Rodrigues ◽  
Tatiana Rojo Moro ◽  
Maria Eugênia Rabello Duarte ◽  
Miguel Daniel Noseda
Keyword(s):  
Cold Water ◽  
2D Nmr ◽  
Water Soluble ◽  
In Vitro Assays ◽  
Pavlova Lutheri ◽  
Alkaline Extract ◽  
Nmr Techniques ◽  
Insoluble Precipitate ◽  
Adhesion Process

Different molecules have been tested as analog receptors due to their capacity to bind bacteria and prevent cell adhesion. By using in vitro assays, the present study characterized the aqueous and alkaline extracts from microalgae Pavlova lutheri and Pavlova gyrans and evaluated the capacity of these extracts to adhere to enterobacteria (Salmonella Typhimurium). The aqueous and alkaline extracts of both species were fractionated via freeze-thawing, giving rise to soluble and insoluble (precipitate) fractions in cold water. The obtained fractions were studied using thermogravimetric, methylation analyses, and using 1D and 2D NMR techniques. The cold-water-soluble fractions obtained from the aqueous extracts were mainly composed of highly branched (1→3),(1→6)-β-glucans, whereas the cold-water-precipitate fractions were constituted by (1→3)-β-glucans. The alkaline extract fractions showed similar compositions with a high protein content, and the presence of glycosides (sulfoquinovosylglycerol (SQG), digalactosylglycerol (DGG)), and free fatty acids. The linear (1→3)-β-glucans and the alkaline extract fractions showed an adhesion capacity toward Salmonella. The chemical composition of the active fractions suggested that the presence of three-linked β-glucose units, as well as microalgal proteins and glycosides, could be important in the adhesion process. Therefore, these microalgal species possess a high potential to serve as a source of anti-adhesive compounds.

scholarly journals Climate, nitrogen and grass. 6. Comparison of yield and chemical composition of some temperate and tropical grass species grown at different temperatures.

1975 ◽  
Vol 23 (1) ◽  
pp. 69-82 ◽  
Author(s):  
B. Deinum ◽  
J.G.P. Dirven
Keyword(s):  
Cell Wall ◽  
Lolium Perenne ◽  
Festuca Arundinacea ◽  
N Fertilization ◽  
Grass Species ◽  
Soluble Carbohydrates ◽  
In Vitro Digestibility ◽  
Tropical Grasses ◽  
Brachiaria Ruziziensis

For part 5 see NAR 43, 148. 6. Lolium perenne, Festuca arundinacea and 2 tropical grasses, Axonopus compressus and Brachiaria ruziziensis, were grown in pots under day/night temperatures of 19 deg /12 deg , 24 deg /18 deg , 28 deg /23 deg and 34 deg /30 deg C and regrowth was cut after 18 and 35 days. The stemmy Brachiaria was much more productive than the others which remained vegetative. The optimum production temperature was 24 deg or less for the temperate grasses but >30 deg for the others. Yield was greatest after 35 days and with high N fertilization. Lolium had the highest average content of N, soluble carbohydrates and digestibility in vitro and Brachiaria the lowest. Digestibility declined with increase in temperature and age, most for Brachiaria. These effects were caused by an increase in content of cell-wall constituents and a decrease in their digestibility. N fertilization had no effect on digestibility. Results support the hypothesis that the low digestibility of tropical grasses is mainly due to the high temperature during growth but may be improved appreciably if stem formation is suppressed. ADDITIONAL ABSTRACT: Lolium perenne, Festuca arundinacea, Axonopus compressus and Brachiaria ruziziensis were grown in pots in a glasshouse under day/night temperatures of 19/12, 24/18, 28/23 or 34/30 deg C with intermediate or high N and regrowth was harvested after 18 and 35 days. Reproductive B. ruziziensis was much more productive than the other vegetative species. Optimum day temperature for production was 24 deg or lower for temperate grasses but >30 deg for tropical species. L. perenne had the highest contents of N and soluble carbohydrates and in vitro digestibility and B. ruziziensis the lowest, differences being smallest at the low temperature. There was a consistent decline in digestibility of all species with increase in temperature and age which was correlated with an increase in cell wall constituents but was not related to amount of N applied. [For Part 5 see HbA 43, 1557] (Abstract retrieved from CAB Abstracts by CABI’s permission)

Controlled Release of Metformin Hydrochloride I. In vitro Release from Physical Mixture Containing Xanthan Gum as Hydrophilic Rate Retarding Polymer

1970 ◽  
Vol 4 (1) ◽  
Author(s):  
Mashkura Ashrafi ◽  
Jakir Ahmed Chowdhury ◽  
Md Selim Reza
Keyword(s):  
Controlled Release ◽  
Xanthan Gum ◽  
In Vitro Release ◽  
Correlation Coefficients ◽  
High Ratio ◽  
Water Soluble ◽  
Metformin Hydrochloride ◽  
Model Drug ◽  
Very High

Capsules of different formulations were prepared by using a hydrophilic polymer, xanthan gum and a filler Ludipress. Metformin hydrochloride, which is an anti-diabetic agent, was used as a model drug here with the aim to formulate sustained release capsules. In the first 6 formulations, metformin hydrochloride and xanthan gum were used in different ratio. Later, Ludipress was added to the formulations in a percentage of 8% to 41%. The total procedure was carried out by physical mixing of the ingredients and filling in capsule shells of size ‘1’. As metformin hydrochloride is a highly water soluble drug, the dissolution test was done in 250 ml distilled water in a thermal shaker (Memmert) with a shaking speed of 50 rpm at 370C &plusmn 0.50C for 6 hours. After the dissolution, the data were treated with different kinetic models. The results found from the graphs and data show that the formulations follow the Higuchian release pattern as they showed correlation coefficients greater than 0.99 and the sustaining effect of the formulations was very high when the xanthan gum was used in a very high ratio with the drug. It was also investigated that the Ludipress extended the sustaining effect of the formulation to some extent. But after a certain period, Ludipress did not show any significant effect as the pores made by the xanthan gum network were already blocked. It is found here that when the metformin hydrochloride and the xanthan gum ratio was 1:1, showed a high percentage of drug release, i.e. 91.80% of drug was released after 6 hours. But With a xanthan gum and metformin hydrochloride ratio of 6:1, a very slow release of the drug was obtained. Only 66.68% of the drug was released after 6 hours. The percent loading in this case was 14%. Again, when Ludipress was used in high ratio, it was found to retard the release rate more prominently. Key words: Metformin Hydrochloride, Xanthan Gum, Controlled release capsule Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

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