Influence of fertilizer treatment on apple fruit composition and physiology. 1. Influence on cell size and cell number.

1961 ◽  
Vol 12 (4) ◽  
pp. 600 ◽  
Author(s):  
DS Letham

Cell size and cell number in Sturmer Pippin apple fruits from trees receiving different fertilizer treatments have been determined. N fruit contained larger cells than U, P, NP, or NPK fruit of the same weight (K = 2 lb sulphate of ammonia, P = 4 lb superphosphate, and K = 1 lb sulphate of potash per tree per year: U = no fertilizer). Fruit from the latter four treatments did not show any consistent differences in cell size. In fruit of the average size for each treatment, differences in cell number per fruit were present,, N fruit containing fewer and larger cells than fruit from all other treatments. Cell number of NPK fruit was greater than that of U fruit. Mean cell volumes for fruit from the various treatments were significantly correlated with the incidences of internal breakdown in storage in each of three years. IS fruit of varieties Cox's Orange Pippin and Dunn's Favourite contained larger cells than U or SPK fruit of the same size. Delicious SPK fruit contained smaller cells than U or IS fruit), the cell sizes of which did not differ.

2021 ◽  
Vol 7 (6) ◽  
pp. 473
Author(s):  
Adel Kamel Madbouly

This study aimed to green synthesize nanosilver (AgNPs) using black tea extract and use it as a nanopreservative to increase the shelf life of stored apple fruits. Ultraviolet visible absorption (UV–vis) analysis of AgNPs recorded two λ max values at 260 and 452 nm. Transmission electron microscope and dynamic light scattering analyses showed that AgNPs are spherical in shape and have an average size of 20 and 170.6 nm, respectively, with a zeta potential of −20.06 mV. An in vitro assay confirmed the antifungal potential of AgNPs against M. fructigena when applied at 200 mg/L and preincubated for 4 days, reducing the radial growth by 96.1%. At the same dose and preincubation period, AgNPs caused a significant reduction in the diameter and fresh weight of brown rotted lesions in apple fruits artificially coinoculated with the pathogen by 77.4% and 84.4%, respectively. AgNPs caused the leakage of proteins and DNA from M. fructigena conidia and did not express cytotoxicity against the human HaCaT cell lines. Accordingly, green synthesized AgNPs are eco-friendly and economical and do not pose harm to human health; thus, they could be used as an effective nanopreservative in apple fruit stores to reduce the incidence of brown rot disease.


1951 ◽  
Vol 4 (2) ◽  
pp. 75 ◽  
Author(s):  
Joan M Bain ◽  
RN Robertson

The problem of fruit size in the Australian apple variety Granny Smith was examined in relation to mean cell size and mean cell number. Cell size gradients in the fruit and changes in cell shape and packing during development were noted.


1952 ◽  
Vol 5 (3) ◽  
pp. 315 ◽  
Author(s):  
D Martin ◽  
TL Lewis

Cell size, total and protein nitrogen, and preclimacteric respiration have been studied for light and heavy crop fruit of certain Tasmanian-grown apple varieties. Differences in size of fruit from light and heavy crops have been shown to be due mainly to differences in cell size rather than in cell number. Respiration per cell, protein nitrogen per cell, and cell volume were closely intercorrelated but respiration per unit protein is greater in light crop fruit than in heavy crop.


1951 ◽  
Vol 4 (2) ◽  
pp. 92 ◽  
Author(s):  
RN Robertson ◽  
JF Turner

The relationship of respiratory activity and quantities of the chemical constituents to fruit size, cell size, and cell number in apple fruits of the variety Granny Smith were examined.


Diabetologia ◽  
2021 ◽  
Author(s):  
Hironobu Sasaki ◽  
Yoshifumi Saisho ◽  
Jun Inaishi ◽  
Yuusuke Watanabe ◽  
Tami Tsuchiya ◽  
...  

Abstract Aims/hypothesis Type 2 diabetes is characterised by reduced beta cell mass (BCM). However, it remains uncertain whether the reduction in BCM in type 2 diabetes is due to a decrease in size or number of beta cells. Our aim was to examine the impact of beta cell size and number on islet morphology in humans with and without type 2 diabetes. Methods Pancreas samples were obtained from 64 Japanese adults with (n = 26) and without (n = 38) type 2 diabetes who underwent pancreatectomy. Using pancreatic tissues stained for insulin, we estimated beta cell size based on beta cell diameter. Beta cell number was estimated from the product of fractional beta cell area and pancreas volume divided by beta cell size. The associations of beta cell size and number with islet morphology and metabolic status were examined. Results Both beta cell size (548.7 ± 58.5 vs 606.7 ± 65.0 μm3, p < 0.01) and number (5.10 × 108 ± 2.35 × 108 vs 8.16 × 108 ± 4.27 × 108, p < 0.01) were decreased in participants with type 2 diabetes compared with those without diabetes, with the relative reduction in beta cell number (37%) being greater than for beta cell size (10%). Beta cell number but not size was positively correlated with BCM in participants with and without type 2 diabetes (r = 0.97 and r = 0.98, both p < 0.01) and negatively correlated with HbA1c (r = −0.45, p < 0.01). Conclusions/interpretation Both beta cell size and number were reduced in participants with type 2 diabetes, with the relative reduction in beta cell number being greater. Decrease in beta cell number appears to be a major contributor to reduced BCM in type 2 diabetes. Graphical abstract


2012 ◽  
Vol 63 (4) ◽  
pp. 463-470 ◽  
Author(s):  
Zdravka Sever ◽  
Dario Ivić ◽  
Tomislav Kos ◽  
Tihomir Miličević

AbstractSeveral species of the genus Fusarium can cause apple fruit to rot while stored. Since Fusarium taxonomy is very complex and has constantly been revised and updated over the last years, the aim of this study was to identify Fusarium species from rotten apples, based on combined morphological characteristics and molecular data.We identified 32 Fusarium isolates from rotten apple fruit of cultivars Golden Delicious, Jonagold, Idared, and Pink Lady, stored in Ultra Low Oxygen (ULO) conditions. Fusarium rot was detected in 9.4 % to 33.2 % of naturally infected apples, depending on the cultivar. The symptoms were similar in all four cultivars: a soft circular brown necrosis of different extent, with or without visible sporulation. Fusarium species were identified by the morphology of cultures grown on potato-dextrose agar (PDA) and carnation leaf agar (CLA). Twenty one isolates were identified as Fusarium avenaceum and confirmed as such with polymerase chain reaction (PCR) using specific primer pair FA-ITSF and FA-ITSR. F. pseudograminearum,F. semitectum, F. crookwellense, and F. compactum were identified by morphological characteristics. F.avenaceum can produce several mycotoxins and its dominance in Fusarium rot points to the risk of mycotoxin contamination of apple fruit juices and other products for human consumption. Pathogenicity tests showed typical symptoms of Fusarium rot in most of the inoculated wounded apple fruits. In this respect Fusarium avenaceum, as the dominant cause of Fusarium rot in stored apple fruits is a typical wound parasite.


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