The root-nodule bacteria as factors in clover establishment in the red basaltic soils of the Lismore district, NSW. I. A survey of "Native" strains.

1954 ◽  
Vol 5 (1) ◽  
pp. 55 ◽  
Author(s):  
JM Vincent
Keyword(s):  
Root Nodule ◽  
New South ◽  
Field Observations ◽  
Nodule Bacteria ◽  
Root Nodule Bacteria ◽  
Seed Inoculation ◽  
South Wales ◽  
Basaltic Soils ◽  
Native Strains ◽  
Medicago Species

Tests of 84 cultures of root-nodule bacteria isolated from four species of clover growing in the Lismore district of New South Wales (three soil types, 18 localities) showed them to be generally effective in association with white and red clovers, but almost always parasitic on subterranean and crimson clovers. Successful seed inoculation with beneficial strains of rhizobia is, therefore, essential to the introduction of subterranean and crimson clovers in these soils. Field observations have also demonstrated the need to inoculate seed of Medicago species, cowpea, and vetch.

The root-nodule bacteria as factors in clover establishment in the red basaltic soils of the Lismore district, NSW. II. Survival and success of inocula in laboratory trials.

1954 ◽  
Vol 5 (1) ◽  
pp. 61 ◽  
Author(s):  
JM Vincent ◽  
LM Waters
Keyword(s):  
Root Nodule ◽  
Nodule Formation ◽  
Nodule Bacteria ◽  
Root Nodule Bacteria ◽  
Reasonable Degree ◽  
Seed Inoculation ◽  
Serological Identification ◽  
Small Rise ◽  
Basaltic Soils ◽  
Native Strains

Plant growth, nature of nodulation, and serological identification of cultures isolated from nodules have been used to determine the success achieved with inocula of clover rhizobia. The Lismore krasnozems offer difficulties in the way of successful seed inoculation. Milk and phosphate supplements with the inoculum do not obviate the difficulty, but heavier rates of inoculation give a reasonable degree of nodulation even with untreated soils. The unfavourable nature of these soils is reflected in their relatively low rhizobial population and the failure of added cells to persist or multiply at a rate comparable with other soils and conditions. Raising pH to c. 7 by the addition of Ca(OH)2 or CaCO3 markedly improves the soil as a medium for survival and multiplication of clover rhizobia. Even a small rise in pH gives appreciable improvement. Strains of root-nodule bacteria differ markedly in their ability to compete with each other for growth and nodule formation on a host. The relative performance of strains can be affected by such factors as the nature of the soil and the presence and nature of the host. Competition for nodulation need not be related in any simple way to competition for growth. Competition by :"native" strains can be an important factor contributing to success or failure with added inoculum.

The effects of high temperatures on certain root-nodule bacteria

1967 ◽  
Vol 18 (2) ◽  
pp. 299 ◽  
Author(s):  
J Wilkins
Keyword(s):  
New England ◽  
High Temperatures ◽  
Root Nodule ◽  
New South ◽  
New South Wales ◽  
Nodule Bacteria ◽  
Natural Conditions ◽  
Root Nodule Bacteria ◽  
South Wales ◽  
Native Strains

Rhizobia present in soils from western New South Wales and from the New England Tableland were subjected to a series of high temperature survival tests. Rhizobia present in air-dry soils survived temperatures higher than would be experienced under natural conditions. In moist soils the tolerance of medic rhizobia to high temperatures was much lower. Strains of Acacia, Lotus and Psoralea rhizobia from western New South Wales survived higher temperatures than strains from the New England Tableland, but the tolerance to high temperatures of medic rhizobia did not vary with source. This suggests an ecological adaptation of the three native strains not found in the introduced Medicago rhizobium.

scholarly journals Stress Tolerance and Symbiotic and Phylogenic Features of Root Nodule Bacteria Associated with Medicago Species in Different Bioclimatic Regions of Tunisia

2011 ◽  
Vol 26 (1) ◽  
pp. 36-45 ◽  
Author(s):  
Salem Djedidi ◽  
Tadashi Yokoyama ◽  
Naoko Ohkama-Ohtsu ◽  
Chandra Prasad Risal ◽  
Chedly Abdelly ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Root Nodule ◽  
Nodule Bacteria ◽  
Root Nodule Bacteria ◽  
Medicago Species

Seed inoculation with effective root-nodule bacteria enhances revegetation success

2005 ◽  
Vol 42 (4) ◽  
pp. 740-751 ◽  
Author(s):  
PETER H. THRALL ◽  
DAVID A. MILLSOM ◽  
ALISON C. JEAVONS ◽  
MEIGAN WAAYERS ◽  
GEOFFREY R. HARVEY ◽  
...  
Keyword(s):  
Root Nodule ◽  
Nodule Bacteria ◽  
Root Nodule Bacteria ◽  
Seed Inoculation

Ecological studies of root-nodule bacteria introduced into field environments. 3. Persistence of Rhizobium trifolii in association with white cover at high elevations

1972 ◽  
Vol 12 (57) ◽  
pp. 407 ◽  
Author(s):  
J Brockwell ◽  
WG Bryant ◽  
RR Gault
Keyword(s):  
White Clover ◽  
Root Nodule ◽  
New South ◽  
Single Step ◽  
Rhizobium Trifolii ◽  
Nodule Bacteria ◽  
Legume Seed ◽  
South Wales ◽  
Inoculant Strain ◽  
High Elevations

Eight strains of Rhizobium trifolii were tested for ability to persist in association with white clover (Trifolium repens) sown at three sites at different elevations in the Snowy Mountains region of New South Wales. The standard clover inoculant strain, TA1, persisted poorly. Five strains of Tasmanian origin (representing two distinct serotypes), all highly effective in nitrogen fixation in combination with white clover, were recovered from the soils at high frequency over a period of three years. A single-step technique of pelleting and seed inoculation was slightly but consistently superior to the more complicated method that has been employed routinely for inoculation of legume seed used in revegetation undertakings in the Snowy Mountains.

Ecological studies of root-nodule bacteria introduced into field environments. I. A survey of field performance of clover inoculants by gel immune diffusion serology

1968 ◽  
Vol 19 (5) ◽  
pp. 739 ◽  
Author(s):  
WF Dudman ◽  
J Brockwell
Keyword(s):  
Root Nodule ◽  
Natural Populations ◽  
Field Performance ◽  
The Other ◽  
Rhizobium Trifolii ◽  
Ecological Studies ◽  
Nodule Bacteria ◽  
Root Nodule Bacteria ◽  
Seed Inoculation ◽  
Clover Seed

A gel immune diffusion procedure for identifying strains of rhizobia has been used to follow the persistence of two strains of Rhizobium trifolii introduced simultaneously into soil by clover seed inoculation. Isolations of rhizobia were obtained from nodulated clover at intervals between 3 and 42 months after sowing. Of 456 isolates examined, 53.3% were recognized as inoculum strains but one strain, TA1, was recovered more than 11 times as frequently as the other, UNZ29. At one place, the inoculum was recovered 30 months after sowing but at another it had disappeared after 18 months. There was a significant trend for the proportion of recoverable inoculum strains to diminish with Iapse of time between sowing and isolation. Even in places where natural populations of Rh. Trifolii were small or undetectable, there was no evidence to suggest that the strains introduced as seed inoculant were likely to become permanently established as a large proportion of the nodule bacteria in the soil.

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