Guanidination procedure increases standardised ileal digestibilities of nitrogen and amino acids in rapeseed meal, soybean meal and peas fed to growing pigs

2013 ◽  
Vol 53 (3) ◽  
pp. 265 ◽  
Author(s):  
M. Eklund ◽  
W. R. Caine ◽  
W. C. Sauer ◽  
G. S. Huang ◽  
G. Diebold ◽  
...  
Keyword(s):  
Amino Acids ◽  
Soybean Meal ◽  
Crude Protein ◽  
Latin Square ◽  
Experimental Period ◽  
Rapeseed Meal ◽  
Antinutritional Factors ◽  
Growing Pigs ◽  
Equal Weight ◽  
Feed Ingredient

A digestibility study was conducted to assess the effect of using the guanidination procedure to convert lysine to homoarginine in dietary sources of protein on standardised ileal digestibilities of nitrogen and amino acids in rapeseed meal, soybean meal or peas fed to growing pigs. Six barrows (German Landrace × Pietrain) with an initial bodyweight of 19 ± 1.8 kg, fitted with ileal T-cannulas, were fed one of three cornstarch-based diets that contained 180 g crude protein/kg (as-fed basis) from rapeseed meal, soybean meal or peas for an experimental period of 10 days according to a double 3 by 3 Latin square design. On Day 9 of each experimental period, the pigs were fed diets containing the guanidinated protein sources, which corresponded to their respective unguanidinated diets. In the guanidinated assay diets, 50% of the dietary source of protein was replaced, on an equal weight basis, with the guanidinated assay feed ingredient. Using the guanidination procedure increased the content of nitrogen in rapeseed meal, soybean meal, and peas by 17, 34, and 8 g/kg DM, respectively. With the exception of lysine in rapeseed meal, using the guanidination procedure increased the standardised ileal digestibilities of most amino acids in rapeseed meal, soybean meal and peas (P < 0.05). Lower standardised ileal digestibililty of lysine in guanidinated compared with unguanidinated rapeseed meal indicates that guanidination was not as uniform compared with the other sources of dietary protein. In conclusion, increases in standardised ileal digestibilities of amino acids after using the guanidination procedure in the aforementioned feed ingredients may be due to solubilisation of protein and degradation of antinutritional factors such as fibre, trypsin inhibitors and tannins.

scholarly journals PSI-12 Effect of a carbohydrase mixture on apparent and standardized ileal digestibility of amino acids in dry extruded-expelled soybean meal fed to growing pigs

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 292-293
Author(s):  
Jichen Song ◽  
Ahmed Aljuobori ◽  
C Martin Nyachoti ◽  
Gustavo A Mejicanos
Keyword(s):  
Soybean Meal ◽  
Mixed Model ◽  
Latin Square ◽  
Experimental Period ◽  
Sole Source ◽  
Antinutritional Factors ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Mean Values ◽  
Ileal Digestibility

Abstract Heat treatment could effectively decrease the antinutritional factors in soybean during meal processing. One such progress is the combination of extrusion with expelling, which produces the dry extruded-expelled soybean meal (DESBM), and there is a great interest in exploring its utilization as a dietary ingredient for swine. Thus, eight ileal-cannulated barrows (initial BW = 30 ± 1.3 kg) were used to determine the ileal amino acid (AA) digestibility of DESBM fed to growing pigs with or without multi-carbohydrase (MC) supplementation. Pigs were randomly assigned to 1 of 4 treatments in a replicated 4 × 4 Latin square design to give eight observations per treatment. The experimental diets were formulated to contain one of the two batches of DESBM (DESBM-1 and DESBM-2) as the sole source of protein. All diets contained titanium dioxide (0.3%) as an indigestible marker to calculate nutrient digestibility. Each experimental period lasted for seven days, and ileal digesta samples were collected on d 6 and d 7. The standardized ileal digestibility (SID) of AA were calculated using published mean values to estimate ileal endogenous AA losses. Data were analyzed using the mixed model procedures of SAS, and the final model had treatment, batch, and the interaction between treatment and batch as the main effects. In general, the SID of AA in DESBM-1 and DESBM-2 were not different, such as Ile, Lys, Met, Thr, and Val, which averaged 88.97%, 89.77%, 89.08%, 84.38%, and 86.85% respectively. There were no effects of MC supplementation on AID and SID of AA digestibility in DESBM except for the AID of Trp (P &lt; 0.05). Also, there were no interaction effects of MC supplementation and batch on AA digestibility in DESBM. In conclusion, enzyme supplementation did not improve the AID and SID of AA in DESBM fed to growing pigs.

Amino acid and energy digestibility in different sources of rice bran for growing pigs

2005 ◽  
Vol 85 (3) ◽  
pp. 355-363 ◽  
Author(s):  
C. Kaufmann ◽  
W. C. Sauer ◽  
M. Cervantes ◽  
Y. Zhang ◽  
J. He ◽  
...  
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Rice Bran ◽  
Soybean Meal ◽  
Latin Square ◽  
Basal Diet ◽  
Experimental Period ◽  
Growing Pigs ◽  
Adaptation Period ◽  
The Individual

Studies were carried out to determine the digestibilities of amino acids (AA) and energy in five sources of rice bran (RB). The sources of RB included two that were not further processed, a defatted, an extruded, and an atypical source of RB consisting mainly of starchy endosperm referred to as polish. Six barrows, average initial body weight 36.5 kg, were fitted with a simple T-cannula at the distal ileum, and fed six diets according to a 6 × 6 Latin square design. The five RB-containing diets contained 53.8% corn, 19.9% soybean meal, and 23.1% RB. The basal diet contained 71.0% corn and 26.3% soybean meal. The dietary allowance was provided at a rate of 4% (wt/wt) of the individual body weight determined at the initiation of each experimental period. Each experimental period comprised 11 d. Following a 7-d adaptation period, faeces were collected for 48 h and ileal digesta for 24 h in two 12-h periods. Chromic oxide was used as the digestibility marker. There was considerable variation in AA digestibilities among the RB samples. By aid of the difference method under the dietary conditions specified, the apparent ileal digestibilities of lysine, methionine, threonine, and tryptophan ranged from 62.6 to 82.2, 61.4 to 76.0, 58.4 to 82.8, and 58.8 to 82.6%, respectively. The digestibilities were lowest in the defatted source of RB and highest in polish. Not including polish of which the total tract energy digestibility was 88.2%, the digestibilities of the other samples ranged from 60.5 to 65.8%. A small proportion of the variation in AA digestibility can be attributed to the content of crude protein, fat and ash in RB. Key words: Amino acids, digestibility, energy, pigs, rice bran

Effect of fermentation of soybean meal with varying protein solubility on ileal digestibility of nutrients in growing pigs

2016 ◽  
Vol 56 (12) ◽  
pp. 2023
Author(s):  
Santi D. Upadhaya ◽  
Je-hoon Ryu ◽  
Kyung-il Kang ◽  
Seong-Jun Cho ◽  
In Ho Kim
Keyword(s):  
Amino Acids ◽  
Soybean Meal ◽  
Crude Protein ◽  
Dry Matter ◽  
Protein Solubility ◽  
Latin Square ◽  
Growing Pigs ◽  
Ileal Digestibility ◽  
Fermented Soybean Meal ◽  
Fermented Soybean

Apparent ileal digestibility and standardised ileal digestibility of energy, dry matter, nitrogen and amino acids were evaluated in five cannulated [(Landrace × Yorkshire) × Duroc)] barrows with an initial bodyweight of 27.1 ± 0.58 kg in a 5 × 5 Latin square design. Pigs were fed unfermented soybean meal (USM) as control and fermented soybean meal A, B and C (FSMA, FSMB and FSMC, respectively), with 52.4%, 62.0% and 71.8% protein solubility, respectively, and a nitrogen-free diet. The nitrogen-free diet was used to determine basal endogenous losses of crude protein and amino acids. The apparent ileal digestibility of dry matter and nitrogen was greater (P < 0.05) in FMSC and tended to improve in FSMA and FSMB compared with the control. The apparent ileal digestibility of isoleucine, phenylalanine and valine was greater in all fermented soybean meal whereas that of lysine was greater (P < 0.05) in FSMC compared with USM. Likewise, the apparent ileal digestibility of aspartic acid and proline was greater (P < 0.05) in FSMA and FSMC. The standardised ileal digestibility of crude protein and lysine was higher (P < 0.05) in FSMC and tended to improve in FSMA and FSMB whereas the standardised ileal digestibility of isoleucine was higher (P < 0.05) in FSMA, FSMB and FSMC compared with USM. In conclusion, fermentation of soybean meal showed better digestibility compared with USM regardless of its protein solubility values.

scholarly journals PSIV-21 Pellet processing reduces variability of corn soybean meal-based diet digestibility in growing pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 175-175
Author(s):  
Élisabeth Chassé ◽  
Frédéric Guay ◽  
Marie-Pierre Létourneau-Montminy
Keyword(s):  
Amino Acids ◽  
Soybean Meal ◽  
Crude Protein ◽  
Dry Matter ◽  
Crossover Design ◽  
Growing Pigs ◽  
Distal Ileum ◽  
Ileal Digestibility ◽  
Pelleted Diet ◽  
The One

Abstract This study was conducted to determine the effect of pelleting on the digestibility of corn-soybean meal-based diet in growing pigs. Two trials with 6 pigs cannulated at the distal ileum were conducted. In each trial, pigs were assigned to each treatment following a crossover design. In each experiment, the same diet, composed of corn and soybean meal with 10% wheat from two different feed mills, was served in pellet or mash form. Pelleting allowed an increase in digestibility in one of the trials. Apparent ileal digestibility (AID) of dry matter (DM), crude protein (CP) and digestible energy (DE) were improved with pelleting by 8, 12 and 9% (P&lt; 0.01). The AID of amino acids (AA) was also improved (P&lt; 0.05). Apparent total tract digestibility (ATTD) was increased by pelleting in DM, CP and DE by 5, 7 and 6% respectively (P&lt; 0.01). The digestibility of the mash diet in experiment 1 was lower than in the pelleted diet in the experiment 1 and both diets in experiment 2 as shown by the interaction Pelleting X Trial which was significant for the AID and ATTD of DM, CP and DE (P&lt; 0.01). Therefore, in experiment 1, pelleting allows to improve the digestibility of diet to the same level as in experiment 2. The AID of CP was higher by 37% in the mash diet from the second experiment compared to the one in the first experiment. Even though the same ingredients were chosen in the two experiments, this shows the variability in digestibility existing between different feed mills and ingredient sources. This difference was not observed in pelleted diets. The results obtained in these two trials show that pelleting can reduce the variability of digestibility and then give a good digestibility of diets even if the ingredients are of different quality or sources.

Effect of dietary fibre level on amino acid digestibility in young pigs

1994 ◽  
Vol 74 (2) ◽  
pp. 327-333 ◽  
Author(s):  
S. Li ◽  
W. C. Sauer ◽  
R. T. Hardin
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Soybean Meal ◽  
Dietary Fibre ◽  
Crude Protein ◽  
Corn Starch ◽  
Experimental Period ◽  
Period Change ◽  
Young Pigs ◽  
Average Body

Studies were carried out with 12 pigs (Yorkshire × Landrace) to determine the effect of dietary fibre level on amino acid digestibility. The pigs were weaned at 21 d of age and fitted with a simple T-cannula at the distal ileum at 23 or 24 d of age. The pigs were fed four isonitrogenous corn starch-based soybean meal diets (22.5 % crude protein) containing 4.3, 7.3, 10.3 and 13.3% Solkafloc according to a balanced two-period change-over design. The pigs were fed four times daily, equal amounts, at 6-h intervals. The diets were supplied at a rate of 5% of the average body weight which was determined at the initiation of the first (8.8 kg) and second (11.7 kg) experimental period. Each experimental period comprised 10 d. Feces were collected for 48 h on days 6 and 7 and ileal digesta for 24 h during days 8, 9 and 10. Chromic oxide was used as digestibility marker. The dietary inclusion of Solkafloc, up to 13.3 %, did not affect (P > 0.05) the apparent ileal as well as the fecal digestibilities of crude protein and amino acids. In conclusion, starter pigs can tolerate up to 13.3% Solkafloc, equivalent to 16.8% neutral-detergent fibre, in corn starch-based soybean meal diets without a depression in amino acid digestibility. Key words: Young pigs, fibre, amino acids, digestibility

scholarly journals Effect of a Multi-Carbohydrase and Phytase Complex on the Ileal and Total Tract Digestibility of Nutrients in Cannulated Growing Pigs

Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1434 ◽  
Author(s):  
Jia-Cheng Yang ◽  
Li Wang ◽  
Ya-Kuan Huang ◽  
Lei Zhang ◽  
Rui Ma ◽  
...  
Keyword(s):  
Organic Matter ◽  
Soybean Meal ◽  
Crude Protein ◽  
Latin Square ◽  
Dietary Supplementation ◽  
Growing Pigs ◽  
Distal Ileum ◽  
Ileal Digestibility ◽  
Gross Energy ◽  
Corn Distillers

The current study evaluated the influence of a multi-carbohydrase and phytase complex (MCPC) on the ileal and total tract digestibility of nutrients in growing pigs. A total of eight barrows (initial BW = 30.7 ± 1.1 kg) were surgically fitted with a T-cannula at the distal ileum and randomly allotted to four groups. The experiment was conducted according to a 4 × 4 Latin square design, each period lasting 10 days. Pigs were fed four experimental diets, which consisted of two basal diets (BD1, low phytate; BD2, high phytate) with or without MCPC containing at least 1800 U xylanase, 6600 U α-arabinofuranosidase, 1244 U β-glucanase, and 1000 U phytase per/kg corn–soybean meal with 15% corn distillers based diet. The high phytate diet reduced (p < 0.05) the apparent ileal digestibility (AID) of crude protein by 1.4% and the apparent total tract digestibility (ATTD) of organic matter, crude protein, and gross energy by 1.7, 2.3, and 1.9%, respectively, and tended to decrease (p = 0.10) the ATTD of Ca by 17.3%, relative to the low phytate diet. The dietary supplementation of the MCPC increased (p < 0.05) the AID of phosphorus (P) and calcium (Ca) by 34.2% and 31.1% for BD1 and 26.7% and 41.3% for BD2, respectively, and increased (p < 0.05) ATTD of crude fat, P, and Ca by 1.4%, 45.6%, and 9.6% for BD1 and 3.1%, 66.0%, and 52.7% for BD2, respectively. The MCPC supplementation did not significantly increase the AID and (or) ATTD of crude protein, organic matter, and starch. In conclusion, the dietary supplementation of the MCPC could improve the AID of P and Ca and the ATTD of crude fat, P, and Ca.

Sunflower expellers have greater ileal digestibility of amino acids than sunflower meal, but there are only minor variations among different sources of sunflower meal when fed to growing pigs

2021 ◽  
Author(s):  
Jimena A Ibagon ◽  
Su A Lee ◽  
Hans H Stein
Keyword(s):  
Amino Acids ◽  
Crude Protein ◽  
Nutritional Value ◽  
Latin Square ◽  
Growing Pigs ◽  
The Other ◽  
Sunflower Meal ◽  
Ileal Digestibility ◽  
Different Sources ◽  
The U.S

Abstract The objective was to test the hypothesis that there is no effect of origin or processing procedure on the standardized ileal digestibility (SID) of amino acids (AA) and crude protein (CP) in sunflower co-products. Six sources of sunflower meal (SFM) and 1 source of sunflower expellers (SFE) were obtained from Ukraine, Italy, Hungary, and the U.S. Each source of SFM or SFE was the only source of CP and AA in 1 diet and an N-free diet was also used for a total of 8 diets. Eight barrows (body weight: 28.5 ± 2.4 kg) had a T-cannula installed in the distal ileum and were allotted to 1 of the 8 diets using an 8 × 8 Latin square design with 8 periods. The Lys:CP ratio in the 6 sources of SFM ranged from 3.10 to 3.96% with CP concentrations ranging from 27.34 to 36.75%. Crude protein in SFE was 26.87% and the Lys:CP ratio was 3.51%. Concentrations of acid hydrolyzed ether extract in the 6 sources of SFM ranged from 0.60 to 3.11%, but SFE contained 8.77%. Results indicated that the SID of CP was lower (P &lt; 0.05) in SFM from Hungary compared with all other sources of SFM except for 1 of the sources from Ukraine. There were no differences in the SID of Lys, Met, and Trp among sources of SFM, but for most of the remaining indispensable AA, the SFM from Hungary had less (P &lt; 0.05) SID than the other sources. However, only few differences in SID of indispensable AA were observed among the other sources of SFM, but the SID of CP and all AA except Trp was greater (P &lt; 0.05) in SFE compared with SFM. In conclusion, there were only few differences in the SID of the first limiting AA among SFM obtained from Ukraine, Hungary, Italy, and the U.S., but the SID of CP and AA was greater in SFE than in SFM indicating that processing of sunflower seeds influence the nutritional value.

scholarly journals The direct and difference procedures result in similar estimates for amino acid digestibility in feed ingredients fed to growing pigs

2020 ◽  
Vol 98 (8) ◽  
Author(s):  
Maryane S F Oliveira ◽  
John K Htoo ◽  
Hans H Stein
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Soybean Meal ◽  
Crude Protein ◽  
Sole Source ◽  
Growing Pigs ◽  
Cereal Grains ◽  
Ileal Digestibility ◽  
Wheat Middlings ◽  
The Difference

Abstract An experiment was conducted to test the hypothesis that values for standardized ileal digestibility (SID) of amino acids (AA) in corn, wheat, and wheat middlings obtained using the direct procedure are not different from values obtained using the difference procedure. Sixteen ileal-cannulated barrows (initial BW: 69.5 ± 5.0 kg) were allotted to a replicated 8 × 4 Youden Square design with 8 diets and 4 periods. Each period consisted of 5 d of adaptation to the diet and 2 d of collection of ileal digesta. Four diets were based on soybean meal (SBM), corn, wheat, or wheat middlings as the only AA-containing ingredients. Three additional diets were based on a mixture of SBM and corn, SBM and wheat, or SBM and wheat middlings, and an N-free diet was also used. The apparent ileal digestibility (AID) and the SID of crude protein (CP) and AA for the 4 diets containing SBM, corn, wheat, or wheat middlings as the sole source of AA were calculated using the direct procedure. The AID and SID of CP and AA for the 3 mixed diets containing SBM and corn, wheat, or wheat middlings were also calculated and the contribution of digestible AA from SBM was subtracted from the AID or SID values for the diets. The AID or SID of AA in corn, wheat, and wheat middlings were subsequently calculated by difference. Results indicated that the AID values for a few AA were lower (P &lt; 0.05) if the direct procedure was used instead of the difference procedure, regardless of ingredient. The AID of Trp was greater in corn and wheat middlings, and the SID of Trp in corn and wheat middlings tended to be greater, if the direct procedure rather than the difference procedure was used, but that was not the case for wheat (interaction, P &lt; 0.05 and P &lt; 0.10, respectively). However, for all other indispensable AA, and for most of the dispensable AA, the SID of AA in corn, wheat, and wheat middlings was not different between the difference procedure and the direct procedure. Therefore, values for SID of AA in cereal grains and fiber-rich ingredients may be determined using either the direct or the difference procedure.

Double-low rapeseed meal for pigs: ileal apparent digestibility of amino acids in diets containing various proportions of rapeseed meal, fish meal and soya-bean meal

1987 ◽  
Vol 44 (3) ◽  
pp. 415-420 ◽  
Author(s):  
I. G. Partridge ◽  
A. G. Low ◽  
J. J. Matte
Keyword(s):  
Amino Acids ◽  
Fish Meal ◽  
Latin Square ◽  
Rapeseed Meal ◽  
Soya Bean ◽  
Growing Pigs ◽  
Main Trial ◽  
Protein Supplements ◽  
Bean Meal ◽  
Soya Bean Meal

ABSTRACTIn order to determine the relative nutritional value of rapeseed meal (var. Tandem) in comparison with soya-bean meal and fish meal, seven barley-based diets were formulated to provide 13·1 MJ digestible energy and 8·8 g total lysine per kg. They contained soya-bean meal (S), rapeseed meal (R) or fish meal (F) alone, or equal proportions (on a total nitrogen basis) of soya and rapeseed, soya and fish meal, rapeseed and fish meal or soya, rapeseed and fish meal. The study involved 14 pigs growing from 40 to 90 kg, each fitted with a T cannula in the terminal ileum. Dietary treatments were applied during 7-day periods according to a 7 × 7 Latin-square design, replicated twice. The pigs were fed at 12-h intervals. Ileal digesta were collected during two 12-h periods from each pig after adaptation to each diet. Nutrient apparent digestibilities were measured using chromium III oxide as a marker; for diets S, R and F, respectively, values were: dry matter, 0·58, 0·51 and 0·66 (s.e. 0·014); N, 0·72, 0·67 and 0·73 (s.e. 0·011); lysine, 0·79, 0·72 and 0·83 (s.e. 0·010), with a similar relationship between diets for most other amino acids. In general, values for diets containing combinations of the protein supplements were intermediate between those for diets containing the respective single supplements. Following the main trial, seven pigs were given a semi-purified diet containing rapeseed meal as the only protein source; this gave amino acid digestibility values similar to or slightly higher than diet R. Diet R, which contained 375 g rapeseed meal per kg, presented no palatability problem. Rapeseed meal of the quality used in this trial could contribute a substantial proportion of the protein supplement for growing pigs. Its value should be enhanced by formulating diets according to the ileal digestibility of the limiting amino acids.

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