scholarly journals Persistence of orally administered Megasphaera elsdenii and Ruminococcus bromii in the rumen of beef cattle fed a high grain (barley) diet

2012 ◽  
Vol 52 (5) ◽  
pp. 297 ◽  
Author(s):  
Athol V. Klieve ◽  
Stuart R. McLennan ◽  
Diane Ouwerkerk

When cattle are fed grain, acidotic ruminal conditions and decreased efficiency in starch utilisation can result from the rapid production and accumulation of lactic acid in the rumen. The efficacy of drenching cattle with Megasphaera elsdenii and Ruminococcus bromii to improve animal performance was investigated. A feedlot trial was undertaken with 80 Bos indicus crossbred steers (initial liveweight 347.1 (s.d. 31.7) kg) in 10 pens in a randomised complete block design. An empty-pen-buffer was maintained between treated (inoculated) and untreated (control) groups to avoid transfer of inoculant bacteria to the control steers. Inoculated steers were orally drenched with M. elsdenii YE34 and R. bromii YE282, and populations increased rapidly over 3–14 days. The steers were fed for a total of 70 days with commercial, barley-based, feedlot rations. High growth rates (1.91 kg per day) were achieved throughout the experiment in both the inoculated and control steers. Intakes averaged 21.3 g dry matter (DM) per kg liveweight per day. There was probably no acidosis achieved in this trial following challenge (i.e. no change in pH occurred). There were no differences in any production or carcass measurements between the control and inoculated steers overall. However, the control group acquired dense ruminal populations of M. elsdenii by Day 14, while R. bromii populations established at high densities within the first 2 weeks but then declined and were undetectable by Day 50. R. bromii appears to be only transiently dominant, and once its dominance waned, it appeared that Ruminobacter spp. established in the rumen. Ruminobacter spp. became dominant between 14 and 28 days in all the steers examined and persisted through to the end of the study. These Ruminobacter spp. may be of future interest in the development of probiotics for grain-fed cattle.

2019 ◽  
Vol 8 (4) ◽  
pp. 257-263
Author(s):  
Arezoo Haseli ◽  
Ashraf Ghiasi ◽  
Mozhgan Hashemzadeh

Introduction: Prolonged labor is a common birth complication that is associated with some negative maternal and fetal effects. The aims of this study were 1) to evaluate the effect of effleurage abdominal massage and 2) to assess the effects size of breathing techniques with massage on the length of labor. Methods: This study was a randomized trial with concealed allocation, assessor blinding for some outcomes and intent-to-treat analysis. Primiparous women (n=117) age 18-35 years who were randomly assigned to three groups; abdominal massage (n=37), abdominal massage with breathing technique (n=38) and control (n=42). Although it was randomized block design with the allocation ratio 1:1:1 but soon after the sample was withdrawn in labor, another was replaced. Experimental groups’ participants received a 30-min effleurage abdominal massage during the active and transitional phases of labor. Particular breathing techniques in each stage of labor were done. Data were analyzed using SPSS ver.13. Results: Duration of the active phase was 244.89(83.30) min in the massage, 254(68.55) min in massage with breathing and 312.07(67.17) min in control group, which was significantly different between the massage and control groups (P<0.001, Min Diff; -67.18), as well as massage with breathing and control groups (P=0.003, Min Diff; -9.63). The Scheffe test showed no significant difference between the two experimental groups. Conclusion: Effleurage abdominal massages decrease length of active phase on labor, but the learning of breathing techniques in labor couldn’t enhance this effect of massage, so it is likely that breathing exercises may be considered during pregnancy.


2020 ◽  
Vol 10 (5) ◽  
pp. 155-159
Author(s):  
T.F. Domatskaya ◽  
A.N. Domatsky ◽  
A.N. Domatsky

The study objective is to study the effectiveness of lactic acid in varroatosis and its impact on the development of bee colonies under conditions of the South of Tyumen region. The acaricidal effectiveness of lactic acid was studied in October 2019 using one method in two experiments in 30 broodless bee colonies kept in two apiaries, where bee colonies were divided into 2 groups (experimental - 10 colonies and control groups of 5 colonies each). For treatment of bee colonies from the experimental group, frames with bees were removed from the hives in turn, which were sprayed with a 15.0% aqueous solution of lactic acid in the volume of 5 ml on each side of the honeycomb. Bee colonies were treated twice with an interval of 5 days. The bees in the control group were not treated. Dead mites were registered on a daily basis in all groups within 5 days after each treatment, for these reasons laminated cardboard sheets were placed on the bottom of the hives, which then were taken out and the number of fallen ectoparasites was recorded. Studies on the impact of lactic acid on the development of bee colonies were conducted in 20 brood bee colonies in the period from May to July 2020. It has been found that the effectiveness of treatment of experimental bee colonies with the drug was 90.0 ± 2.1% (apiary No 1) and 7.8 ± 1.9% (apiary No 2). At the same time, the maximum death of mites in colonies was observed during the first day after treatment. In the control groups, the number of mites decreased by 10.9 ± 0.8% and 11.6 ± 1.0%, respectively. It has been established that the treatment of bees with an aqueous solution of lactic acid in the above concentration, dose and multiplicity did not have a negative impact on the development of bee colonies, their strength and food supply, and no deaths of bees and queens were detected during the observation period.


2007 ◽  
Vol 19 (1) ◽  
pp. 151
Author(s):  
K. Nagano ◽  
J. Todoroki ◽  
M. Imamura ◽  
C. Kubota

We investigated the safety of somatic cell-cloned Holstein cows and the analogy (growth and milk performance) of the cloned cows and their progeny. Individually, the cloned cows were produced using the same fibroblasts of a Holstein cow as the donor cells. All cows were fed in accordance with NRC (1999) standards during the experimental period. Three cloned cows (clone group), their 3 progeny (progeny group), and 3 cows produced with artificial insemination (AI, control group) were reared under general group feeding. Viability, fertility, milk yield,and blood characteristics in clone, progeny, and control groups were examined. Statistical analyses were carried out by Student&apos;s t-test. The birth weight in the clone group (55.2 &plusmn; 6.3 kg (mean &plusmn; SD)) was heavier than that of the control group (44.8 &plusmn; 5.6 kg (P &gt; 0.05)); it was also heavier than that of an upper limit (51.0 kg) and standard value (40.0 &plusmn; 5.8 kg) of Holstein heifers in Japan. However, no significant change in the amount of dry matter intake per kg body weight/day was observed between the clone group (0.97 &plusmn; 0.06 kg) and the control group (0.9 &plusmn; 0.02 kg). As to blood characteristics in the raising phase, the number of red blood cells in the clone group (684.8 &plusmn; 16.8 &times; 104 &micro;L) was decreased slightly (P &lt; 0.01) compared with that in the control group (823.3 &plusmn; 26.1 &times; 104 &micro;L). However, there were no differences in other blood test items between clone and control groups. Furthermore, we found that there were no differences in estrous cycles and plasma progesterone concentrations between clone and control groups. All cloned and control cows that had been inseminated became pregnant and delivered normally. During the period from 21 days before expected date of confinement to 10 days after parturition, relative to the blood characteristics, the number of blood cells in the clone group (499.9 &plusmn; 77.5 &times; 104 &micro;L) tended to be lower (P &lt; 0.01), when compared to that in the control group (711.2 &plusmn; 39.9 &times; 104 &micro;L); no differences were observed in plasma estrogen and progesterone concentrations between clone and control groups throughout the measurement period. In the first lactation period, there were no differences in total amount of milk produced in the first 305 days of lactation, milk percentage, milk protein rate, and solids-not-fat between clone and control groups. The total amount of milk produced in the first 365 days in cloned cows was almost equal to that of the original cow who provided donor cells. In 3 progeny of the cloned cows, the average birth weight was not different from that in the control group and the standard growth value. In the raising period, there were no differences in changes of the weight and height of a body and of the dry matter intake per day, and in the blood characteristics between progeny and control groups. Conceivably, our results suggest that the cloned Holstein cows and their progeny have normal growth, reproductive, and lactation characteristics, although the birth weights of the cloned cows were larger than those of control cows.


2010 ◽  
Vol 80 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Pei-Min Chao ◽  
Wan-Hsuan Chen ◽  
Chun-Huei Liao ◽  
Huey-Mei Shaw

Conjugated linoleic acid (CLA) is a collective term for the positional and geometric isomers of a conjugated diene of linoleic acid (C18:2, n-6). The aims of the present study were to evaluate whether levels of hepatic α-tocopherol, α-tocopherol transfer protein (α-TTP), and antioxidant enzymes in mice were affected by a CLA-supplemented diet. C57BL/6 J mice were divided into the CLA and control groups, which were fed, respectively, a 5 % fat diet with or without 1 g/100 g of CLA (1:1 mixture of cis-9, trans-11 and trans-10, cis-12) for four weeks. α-Tocopherol levels in plasma and liver were significantly higher in the CLA group than in the control group. Liver α-TTP levels were also significantly increased in the CLA group, the α-TTP/β-actin ratio being 2.5-fold higher than that in control mice (p<0.01). Thiobarbituric acid-reactive substances were significantly decreased in the CLA group (p<0.01). There were no significant differences between the two groups in levels of three antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). The accumulation of liver α-tocopherol seen with the CLA diet can be attributed to the antioxidant potential of CLA and the ability of α-TTP induction. The lack of changes in antioxidant enzyme protein levels and the reduced lipid peroxidation in the liver of CLA mice are due to α-tocopherol accumulation.


1993 ◽  
Vol 30 (2) ◽  
pp. 227-230 ◽  
Author(s):  
Andrew Mccance ◽  
David Roberts-Harry ◽  
Martyn Sherriff ◽  
Michael Mars ◽  
William J.B. Houston

The study models of a group of adult Sri Lankan patients with clefts of the secondary palate were investigated. Tooth-size and arch-dimension comparisons were made with a comparable control group. Significant differences were found between the cleft and control groups in tooth sizes, chord lengths, and arch widths. The cleft group dimensions were generally smaller than those of the control group. Overjets were larger in the cleft group.


Author(s):  
Hasanul Arifin Zul And Masitowarni Siregar

This thesis is focused on the investigation of the effect of applying animal cartoon pictures on students’ achievement in writing narrative text. This study aims to find whether applying animal cartoon pictures significantly affect the students’ writing achievement or not. The data in this study were obtained by administering a written test. The population was the 2015/2016 first year (grade XI) of SMA Swasta Nusantara Lubuk Pakam and 66 students were taken as the sample by using random sampling. The sample was divided into two groups, experimental and control groups. The experimental group was taught by applying animal cartoon pictures while the control group without animal cartoon pictures (x = lecturing). The data were taken the scores from the pre-test and post-test to both experimental and control groups. These data were analyzed by using t-test. The result of computing the t-test obviously showed that t-observed is higher than t-table (5.21 >1,67) with the degree of freedom 64 (df =N-2) at the level significance 0,05 one tail test. It showed that the application of animal cartoon pictures significantly affected the students of SMA Swasta Nusantara Lubuk Pakam achievement in writing narrative text.


Author(s):  
Meryanti Napitupulu And Anni Holila Pulungan

This study was conducted as an attempt to discover the effect of applying Demonstration Method on students’ achievement in speaking skill. It was an experimental research. The subject was students of Grade XII, Vocational High School (Sekolah Menengah Kejuruan: SMK), which consisted of 79 students. The research was divided into two groups: experimental and control groups. The instrument used to collect the data was speaking test. To obtain the reliability of the test, the writer applied Kuder Richardson 21 formula. The result of the reliability was 0.7, and it was found that the test was reliable. The data were analyzed by using t-test formula. The analysis showed that the scores of the students in the experimental group were significantly higher than the scores of the students in the control group at the level of significant m = 0.05 with the degree of freedom (df) 77, t-observed value 8.9 > t-table value 1.99. The findings indicate that using Demonstration Method significantly affected the students’ achievement in speaking skill. So, English teachers are suggested to use Demonstration Method in order to improve students’ achievement in speaking skill.


2016 ◽  
Vol 1 (1) ◽  
pp. 22
Author(s):  
Nazli Zainuddin ◽  
Nurul Azira Mohd Shah ◽  
Rosdan Salim

Introduction: The role of virgin coconut oil in the treatment of allergic rhinitis is controversial. Thus, the aim of the present study is to determine the effects of virgin coconut oil ingestion, in addition to standard medications, on allergic rhinitis. We also studied the side effects of consumption of virgin coconut oil. Methods: Fifty two subjects were equally divided into test and control groups. All subjects received a daily dose of 10mg of loratadine for 28 days. The test group was given 10ml of virgin coconut oil three times a day in addition to loratadine. The symptoms of allergic rhinitis were scored at the beginning and end of the study. Results:, the symptom score were divided into nasal and non-nasal symptom scores. Sneezing score showed a significant difference, however the score was more in control group than test group, indicating that improvement in symptom was more in control group. The rest of the nasal symptom and non-nasal symptom score showed no significant difference between test and control groups. Approximately 58% of the test subjects developed side effects from consumption of virgin coconut oil, mainly gastrointestinal side effects. Conclusion: In the present study, ingestion of virgin coconut oil does not improve the overall and individual symptoms of allergic rhinitis, furthermore it has side effects.


1984 ◽  
Vol 58 (1) ◽  
pp. 23-30
Author(s):  
Donald S. Martin ◽  
Ming-Shiunn Huang

The actor/observer effect was examined by Storms in a 1973 study which manipulated perceptual orientation using video recordings. Storms' study was complex and some of his results equivocal. The present study attempted to recreate the perceptual reorientation effect using a simplified experimental design and an initial difference between actors and observers which was the reverse of the original effect. Female undergraduates performed a motor co-ordination task as actors while watched by observers. Each person made attributions for the actor's behaviour before and after watching a video recording of the performance. For a control group the video recording was of an unrelated variety show excerpt. Actors' initial attributions were less situational than observers'. Both actors and observers became more situational after the video replay but this effect occurred in both experimental and control groups. It was suggested the passage of time between first and second recording of attributions could account for the findings and care should be taken when interpreting Storms' (1973) study and others which did not adequately control for temporal effects.


2019 ◽  
Vol 1 (2) ◽  
pp. 47-55
Author(s):  
Syahredi Syaiful Adnani ◽  
Hafni Bachtiar

In the last few decades, the incidence of caesarean section is increasing in the world, especially in Indonesia. One of the way to treat tissue scar is through biologic and synthetic dressing where nowadays, amnion has been used as biologic dressing frequently. This study was conducted to determine the effect of the use of fresh amniotic membrane on wound incision Caesarean section compared with Caesarean section incision wound covered using regular gauze bandages and fixated with plaster in RS. Dr. Reksodiwiryo Padang. The design of this study is an experimen-tal study with Post test design with control group design. Sampling was done using a formula consecutive sampling two different test samples obtained an average of 72 people for each group. The analysis used include univariate and bivariate analyzes. The average wound healing time the difference was statistically significant (p value <0.05) in the treatment and control groups. There was highly significant difference in the proportion of local infection on day 3 between the treatment and control groups (p value <0.05). There were very significant differences in the proportion of local allergic reactions at day 3, and 5 between the treatment and control group (p <0.05). There are significant differences in terms of the cost of care per day between treatment and control groups (p <0.05). From this study, the average wound healing time has a very significant difference.Keywords: Fresh Amniotic Membrane, Wound Cesarean Section, Wound Healing


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