Liver and kidney function analysis on mice given ethyl acetate fraction of Garcinia mangostana as potential colorectal cancer therapeutic agent

2019 ◽  
Author(s):  
Andika Trya ◽  
M. Wien Wienarno ◽  
Kamarza Mulia ◽  
M. Idzhar Arridzal ◽  
Erni Hernawati Purwaningsih ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Ethyl Acetate ◽  
Kidney Function ◽  
Therapeutic Agent ◽  
Function Analysis ◽  
Ethyl Acetate Fraction ◽  
Garcinia Mangostana ◽  
Liver And Kidney

AKTIVITAS ANTIBAKTERI DAN ANALISIS KLT-BIOAUTOGRAFI DARI FRAKSI DAUN MANGGIS (Garcinia mangostana L.)

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 525
Author(s):  
Ayu Natasya Paputungan ◽  
Widya Astuty Lolo ◽  
Imam Jayanto
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Garcinia Mangostana ◽  
Methanol Fraction ◽  
Tlc Bioautography ◽  
Fractionation Method

Mangosteen leaves have flavonoid compounds, tannins, and saponins that can be efficacious as antibacterial. The aim of this study was to determine the fraction of mangosteen leaves having an antibacterial effect and knowing the class of compounds identified as having antibacterial activity after TLC- Bioautography testing was carried out. The samples were extracted using 96% maceratarion method and fractioned using liquid-liquid fractionation method with methanol, n-hexane and ethyl acetate solvents, antibacterial activity using agar diffusion method (Kirby and Bauer) with 3 concetrations namely 10%, 20% and 30%. Thin Layer Chromatography (TLC) uses n-hexane and chloroform solvens. TLC-Bioautography uses contact bioautography methods. The resultd showed that mangosteen leaves in methanol fraction with a concentration of 30% had a very large inhibitory activity again Staphylococcus aureus and ethyl acetate fraction with a concentration of 30% had the gratest antibacterial activity against  Escherichia coli. The results of the TLC- Bioautography study showed that the flavonoids compounds after spraying with AlCl3 and the mangosteen leaf Biosutography test had inhibitory zone activity against the bacteria Staphylococcus aureus dan Escherichia coli. Keywords: Mangosteen Leaves. Antibacterial, TLC Bioautography.  ABSTRAK Daun manggis mempunyai senyawa flavonoid, tanin, dan saponin yang dapat berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui fraksi daun manggis memiliki efek antibakteri dan mengetahui golongan senyawa yang teridentifikasi memiliki aktivitas antibakteri setelah dilakukan pengujian KLT Bioautografi. Sampel diektrak dengan metode maserasi dengan pelarut 96% dan difraksinasi dengan metode  fraksinasi cair-cair dengan pelarut metanol, n-heksan dan etil asetat, aktivitas antibakteri menggunakan metode difusi agar (Kirby and Bauer) dengan 3 kosentrasi yaitu 10%, 20% dan 30%. Kromatografi Lapis Tipis (KLT) menggunakan pelarut n-heksan dan klorofom. KLT-Bioautografi menggunakan metode bioautografi kontak. Hasil penelitian menunjukan daun manggis pada fraksi metanol  dengan kosentrasi 30% memiliki aktivitas zona hambat ppaling besar terhadapat bakteri Staphylococcus aureus dan fraksi etil asetat dengan kosentrasi 30% memiliki aktivitas antibakteri paling besar terhadap  Escherichia coli. Hasil penelitian KLT-Bioautografi menunjukan golongan senyawa flavonoid setelah disemprotkan dengan AlCl3 dan uji Bioautografi daun manggis memiliki aktivitas zona hambat terhadap bakteri Staphylococcus aureus dan Escherichia coli. Kata kunci : Daun Manggis, Antibakteri, KLT- Bioautografi.

scholarly journals Acute Oral Toxicity Testing of Ethyl Acetate Fraction from Garcinia mangostana Linn Extract in Sprague-Dawley Rats

2016 ◽  
Vol 10 (3) ◽  
pp. 261-264 ◽  
Author(s):  
F. Rahmayanti ◽  
D.F. Suniarti ◽  
Z.A. Mas`ud ◽  
B.M. Bachtiar ◽  
Y.S. Wimardhani ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Toxicity Testing ◽  
Ethyl Acetate Fraction ◽  
Acute Oral Toxicity ◽  
Sprague Dawley ◽  
Oral Toxicity ◽  
Garcinia Mangostana ◽  
Sprague Dawley Rats

scholarly journals Assessment of Self-Nanoemulsifying Drug Delivery System (SNEDDS) of Ethyl Acetate Fraction from Mangosteen (Garcinia mangostana L.,) Peels to Eschericia coli, Pseudomonas aeroginosa, and Proteus mirabilis

2019 ◽  
Vol 24 (3) ◽  
pp. 189 ◽  
Author(s):  
Liza Pratiwi
Keyword(s):  
Drug Delivery ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Drug Delivery System ◽  
Delivery System ◽  
Proteus Mirabilis ◽  
Ethyl Acetate Fraction ◽  
Garcinia Mangostana ◽  
Diabetic Ulcers ◽  
Ethanol Extracts

Mangosteen peels have antibacterial activity. SNEDDS has many advantages in developing a drug delivery system to increase the penetration of active compounds. The purpose of this study was to compare the effectiveness of the antibacterial SNEDDS of ethyl acetate fraction from mangosteen peels and ethyl acetate fraction of mangosteen peels without SNEDDS preparation as an antibacterial against Eschericia coli, Pseudomonas aeroginosa, and Proteus mirabilis, that cause diabetic ulcers. This research began with maceration. The thick ethanol extracts were continued and fractionation was carried out with ethyl acetate solvents then was formulated into SNEDDS. The measurement of the antibacterial activity with the bacterial growth inhibition parameters of SNEDDS preparations extracted from ethyl acetate fraction of mangosteen peels was compared with ethyl acetate fraction of mangosteen peels without SNEDDS preparation. Data were analyzed using independent sample T-Test. The results showed the SNEDDS preparation of ethyl acetate fraction from mangosteen peel had activity against both types of bacteria causing diabetic ulcers, but it had no activity against Proteus mirabilis. The results of statistical analysis showed that there were significant differences in the activity of SNEDDS ethyl acetate fraction of mangosteen peels and ethyl acetate fraction of mangosteen peels without SNEDDS in Eschericia coli and Pseudomonas aeruginosa.

scholarly journals Hepatoprotective, nephroprotective, anti-amylase, and antiglucosidase effects of Ziziphus spina-christi (L.) against carbon tetrachloride-induced toxicity in rats

2021 ◽  
Vol 18 (4) ◽  
pp. 781-790
Author(s):  
Amal Ahmed Mohammed Al-Ghamdi ◽  
Manal El-Zohri ◽  
Abdelaaty A. Shahat
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Histopathological Examination ◽  
Ethyl Acetate Fraction ◽  
Group Vi ◽  
Group V ◽  
Aqueous Fraction ◽  
Protein Levels ◽  
Group I ◽  
Liver And Kidney

Purpose: To explore the hepatoprotective, nephroprotective, anti-amylase, and anti-glucosidase effects of the medicinal plant Ziziphus spina-christi (L.). Methods: Ziziphus spina-christi (L.) methanol extract (ZS-1) and its ethyl-acetate (ZS-2), n-butanol (ZS3), and aqueous (ZS-4) fractions were evaluated for their hepatoprotective, anti-amylase, and antiglucosidase activities. Adult male Wister rats were divided into 11 groups (I- XI) with 6 mice per group. Group I was normal control, while the treatment groups were as follows: group II, CCl4; group III, Silymarin + CCl4; group IV, Ziziphus spina-christi total methanol extract (ZS-1), 100 mg/kg) + CCl4; group V, ZS-1 (200 mg/kg) + CCl4; group VI, ethyl acetate fraction (ZS-2), 100 mg/kg + CCl4; group VII: ZS-2 (200 mg/kg) + CCl4; group VIII, butanol fraction (ZS-3), 100 mg/kg) + CCl4; group IX, ZS-3 (200 mg/kg) + CCl4; group X, aqueous fraction (ZS-4), 100 mg/kg) + CCl4; group XI: ZS-4 (200 mg/kg) + CCl4. Silymarin was used as the standard. Biomarkers of liver and kidney toxicity and histopathological changes were evaluated. Results: Liver and kidney malondialdehyde (MDA), non-protein sulfhydryls (NP-SH) and total protein levels were elevated in CCl4-treated rats; however, ZS-1 and ZS-4 of Z. spina-christi significantly reduced these levels. ZS-2 and ZS-3 did not significantly improve the studied parameters. These results were confirmed by results from histopathological examination. ZS-1 and ZS-2 showed mild inhibitory activities against α-amylase and α-glucosidase (54 and 43 % at 100 µg/ml, respectively). Conclusion: The results indicate that ZS-1 and ZS-4 samples displayed dose-dependent hepatoprotective and nephroprotective effects, whereas ZS-2 and ZS-3 samples did not exhibit these effects. Similarly, α-amylase and α-glucosidase enzymes are considerably inhibited by ZS-1 and ZS-2.

scholarly journals Ethanol Extract, Ethyl Acetate Extract, Ethyl Acetate Fraction, and n-Heksan Fraction Mangosteen Peels (Garcinia mangostana L.) As Source of Bioactive Substance Free-Radical Scavengers

2016 ◽  
Vol 1 (2) ◽  
pp. 71
Author(s):  
Liza Pratiwi ◽  
Achmad Fudholi ◽  
Ronny Martien ◽  
Suwidjiyo Pramono
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Garcinia Mangostana ◽  
Ic50 Value ◽  
Dpph Method ◽  
Peel Extract

<span>Mangosteen peels (Garcinia mangostana L.) is well known as an excellent source of antioxidative compounds. The name of mangosteen is xanthone. Antioxidant of mangosteen  peels can be extracted by ethanol, etyl acetate and can be fractinated by etyl acetate and n-hexane. The aim of this research was to compare the antioxidant activity of the peel extract by ethanol and etyl acetate and fractinated by etyl acetate and n-hexane. Extract and fraction exhibited higher scavenging activity of DPPH. </span><span lang="EN">The purpose of this study was to compare antioxidant activity of ethanol extract, ethyl acetate extract, fraction of ethyl acetate and n-hexane fraction.</span><span> The</span><span lang="EN"> antioxidant activity </span><span>test </span><span lang="EN">using DPPH method with UV-Vis spectrophotometer</span><span>. Ethanol extract shown IC50 value 5,03 µg/mL, ethyl acetate extract shown IC50 value 41,56 µg/mL. Ethyl acetate fraction shown IC50 value 2,78 µg/mL, and n-hexane fraction with IC50 22,33 µg/mL. It means peel extract and fraction by mangosteen peels has very strong antioxidant activity and ethyl acetate fraction that its antioxidant activity higher that the other solvent.</span>

Assessment of Antimicrobial Activity and total Polyphenol Content of Rosa multiflora and Rosa wichuraiana

2019 ◽  
Vol 10 (04) ◽  
pp. 646-650
Author(s):  
Hyeusoo Kim ◽  
Kyeong Won Yun
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Antimicrobial Agents ◽  
Ethyl Acetate Fraction ◽  
Rosa Multiflora ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Water Fraction ◽  
Total Polyphenol Content ◽  
Rosa Species

The fruit of Rosa multiflora has been used as traditional herbal medicine in Asian countries. The present investigation was undertaken to study the antimicrobial activity and total polyphenol content of hexane, ether, ethyl acetate, water fraction of methanol extract of fruit and flower from Rosa multiflora and Rosa wichuraiana. Antimicrobial activity of the mentioned fractions against 3 gram-positive and 4 gram-negative bacteria using disk diffussion method. The measurement of minimal inhibition concentration (MIC) showed that the ethyl acetate fraction of the two Rosa species is the most effective against the tested bacteria. The total polyphenol content of ethyl acetate fraction of the two Rosa species is higher than the other fractions. The results indicate the antimicrobial activity was related with the total polyphenol content and the fruit and flower of the two Rosa species can be considered as a natural source of antimicrobial agents.

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