Controlling microarray DNA hybridization efficiency by probe-surface distance and external surface electrostatics

2015 ◽  
Author(s):  
K. Qamhieh ◽  
B. Montgomery Pettitt
Keyword(s):  
Dna Hybridization ◽  
External Surface ◽  
Probe Surface ◽  
Surface Distance ◽  
Hybridization Efficiency ◽  
Microarray Dna

scholarly journals Correlation between microarray DNA hybridization efficiency and the position of short capture probe on the target nucleic acid

BioTechniques ◽  
2005 ◽  
Vol 39 (1) ◽  
pp. 89-96 ◽  
Author(s):  
Régis Peytavi ◽  
Liu-ying Tang ◽  
Frédéric R. Raymond ◽  
Karel Boissinot ◽  
Luc Bissonnette ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Hybridization ◽  
Capture Probe ◽  
Target Nucleic Acid ◽  
Hybridization Efficiency ◽  
Microarray Dna

Optimization of DNA Hybridization Efficiency by pH-Driven Nanomechanical Bending

Langmuir ◽  
2012 ◽  
Vol 28 (15) ◽  
pp. 6494-6501 ◽  
Author(s):  
Jiayun Zhang ◽  
Hans Peter Lang ◽  
Genki Yoshikawa ◽  
Christoph Gerber
Keyword(s):  
Dna Hybridization ◽  
Hybridization Efficiency

DNA Hybridization Efficiency on Concave Surface Nano-Structure in Hemispherical Janus Nanocups

Langmuir ◽  
2014 ◽  
Vol 30 (5) ◽  
pp. 1272-1280 ◽  
Author(s):  
Hyonchol Kim ◽  
Hideyuki Terazono ◽  
Hiroyuki Takei ◽  
Kenji Yasuda
Keyword(s):  
Dna Hybridization ◽  
Concave Surface ◽  
Nano Structure ◽  
Hybridization Efficiency

scholarly journals DNA-tethered Polymersome Clusters as Nanotheranostic Platform

2021 ◽  
Vol 75 (4) ◽  
pp. 296-299
Author(s):  
Claire E. Meyer ◽  
Cora-Ann Schoenenberger ◽  
Juan Liu ◽  
Ioana Craciun ◽  
Cornelia G. Palivan
Keyword(s):  
Dna Hybridization ◽  
Molecular Level ◽  
External Surface ◽  
Cluster Formation ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Diagnosis And Treatment ◽  
Active Compounds ◽  
Complementary Dna ◽  
Dna Strands

Nanotheranostics combine the use of nanomaterials and biologically active compounds to achieve diagnosis and treatment at the same time. To date, severe limitations compromise the use of nanotheranostic systems as potent nanomaterials are often incompatible with potent biomolecules. Herein we emphasize how a novel type of polymersome clusters loaded with active molecules can be optimized to obtain an efficient nanotheranostic platform. Polymersomes loaded with enzymes and specific dyes, respectively and exposing complementary DNA strands at their external surface formed clusters by means of DNA hybridization. We describe factors at the molecular level and other conditions that need to be optimized at each step of the cluster formation to favor theranostic efficiency.

scholarly journals Enhancement of target-DNA hybridization efficiency by pre-hybridization on sequence-orientated micro-arrayed probes

2008 ◽  
Vol 39 (3) ◽  
pp. 187-193 ◽  
Author(s):  
Dan-Kai Yang ◽  
Jie-Len Huang ◽  
Chia-Chun Chen ◽  
Hung-Ju Su ◽  
Jui-Chuang Wu
Keyword(s):  
Dna Hybridization ◽  
Target Dna ◽  
Hybridization Efficiency

scholarly journals The Implications of Fragmented Genomic DNA Size Range on the Hybridization Efficiency in NanoGene Assay

Sensors ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 2646 ◽  
Author(s):  
Xiaofang Wang ◽  
Beelee Chua ◽  
Ahjeong Son
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Dna Hybridization ◽  
Genomic Dna ◽  
Size Range ◽  
Optimum Size ◽  
Hybridization Efficiency ◽  
Dna Size

DNA hybridization-based assays are well known for their ability to detect and quantify specific bacteria. Assays that employ DNA hybridization include a NanoGene assay, fluorescence in situ hybridization, and microarrays. Involved in DNA hybridization, fragmentation of genomic DNA (gDNA) is necessary to increase the accessibility of the probe DNA to the target gDNA. However, there has been no thorough and systematic characterization of different fragmented gDNA sizes and their effects on hybridization efficiency. An optimum fragmented size range of gDNA for the NanoGene assay is hypothesized in this study. Bacterial gDNA is fragmented via sonication into different size ranges prior to the NanoGene assay. The optimum size range of gDNA is determined via the comparison of respective hybridization efficiencies (in the form of quantification capabilities). Different incubation durations are also investigated. Finally, the quantification capability of the fragmented (at optimum size range) and unfragmented gDNA is compared.

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