Fabrication of optical multilayer for two-color phase plate in super-resolution microscope

2014 ◽  
Vol 85 (7) ◽  
pp. 073701
Author(s):  
Yoshinori Iketaki ◽  
Katsuichi Kitagawa ◽  
Kohjiro Hidaka ◽  
Naoki Kato ◽  
Akira Hirabayashi ◽  
...  
Keyword(s):  
Super Resolution ◽  
Phase Plate

Super-resolution in focusing system by three-zone pure phase plate

2009 ◽  
Author(s):  
Xiumin Gao ◽  
Qiufang Zhan ◽  
Jinsong Li ◽  
Jian Wang ◽  
Songlin Zhuang
Keyword(s):  
Super Resolution ◽  
Phase Plate ◽  
Pure Phase

Theoretical analysis of lateral resolution given by annular super-resolution phase plate

2017 ◽  
Author(s):  
Hiroshi Kumagai ◽  
Yoshinori Iketaki ◽  
Koumei Nagai ◽  
Nador Bokor
Keyword(s):  
Theoretical Analysis ◽  
Super Resolution ◽  
Lateral Resolution ◽  
Phase Plate

Characteristics of an Electrostatic Phase Plate

1972 ◽  
Vol 30 ◽  
pp. 618-619
Author(s):  
William Krakow ◽  
Benjamin Siegel
Keyword(s):  
Phase Contrast ◽  
Objective Lens ◽  
Phase Plate ◽  
Net Charge ◽  
Phase Plates ◽  
Beam Currents ◽  
Contrast Image ◽  
Bright Phase ◽  
Additional Phase Shift ◽  
Additional Phase

Unwin has used a metallized non-conducting thread in the back focal plane of the objective lens that stops out a portion of the unscattered beam, takes on a localized positive charge and thus produces an additional phase shift to give a different transfer function of the lens. Under the particular conditions Unwin used, the phase contrast image was shifted to bright phase contrast for optimum focus.We have investigated the characteristics of this type of electrostatic phase plate, both analytically and experimentally, as functions of the magnitude of charge and defocus. Phase plates have been constructed by using Wollaston wire to mount 0.25μ diameter platinum wires across apertures ranging from 50 to 200μ diameter and vapor depositing SiO and gold on the mounted wires to give them the desired charging characteristics. The net charge was varied by adjusting only the bias on the Wehnelt shield of the gun, and hence the beam currents and effective size of the source.

Super-Resolution Microscopy in Studying the Structure and Function of the Cell Nucleus

Acta Naturae ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 42-51
Author(s):  
S. S. Ryabichko ◽  
◽  
A. N. Ibragimov ◽  
L. A. Lebedeva ◽  
E. N. Kozlov ◽  
...  
Keyword(s):  
Cell Nucleus ◽  
Super Resolution ◽  
Structure And Function ◽  
And Function ◽  
Super Resolution Microscopy
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