Evaluation of a combined two-color phase plate forming three-dimensional dark holes in super resolution microscopy

2020 ◽  
Author(s):  
Koumei Nagai ◽  
Maruyama Takashi ◽  
Akira Kodaira ◽  
Hiroshi Kumagai ◽  
Nandor Bokor ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Super Resolution ◽  
Phase Plate ◽  
Super Resolution Microscopy

Cubic spline-based depth-dependent localization of mitochondria–endoplasmic reticulum contacts by three-dimensional light-sheet super-resolution microscopy

The Analyst ◽  
2021 ◽  
Author(s):  
Yucheng Sun ◽  
Seungah Lee ◽  
Seong Ho Kang
Keyword(s):  
Endoplasmic Reticulum ◽  
Calcium Homeostasis ◽  
Three Dimensional ◽  
Super Resolution ◽  
Light Sheet ◽  
Cubic Spline ◽  
Contact Distance ◽  
Super Resolution Microscopy

The contact distance between mitochondria (Mito) and endoplasmic reticulum (ER) has received considerable attention owing to their crucial function in maintaining lipid and calcium homeostasis. Herein, cubic spline algorithm-based depth-dependent...

scholarly journals Small-Molecule Labeling of Live Cell Surfaces for Three-Dimensional Super-Resolution Microscopy

2014 ◽  
Vol 136 (40) ◽  
pp. 14003-14006 ◽  
Author(s):  
Marissa K. Lee ◽  
Prabin Rai ◽  
Jarrod Williams ◽  
Robert J. Twieg ◽  
W. E. Moerner
Keyword(s):  
Small Molecule ◽  
Three Dimensional ◽  
Super Resolution ◽  
Live Cell ◽  
Cell Surfaces ◽  
Super Resolution Microscopy

scholarly journals A bisected pupil for studying single-molecule orientational dynamics and its application to three-dimensional super-resolution microscopy

2014 ◽  
Vol 104 (19) ◽  
pp. 193701 ◽  
Author(s):  
Adam S. Backer ◽  
Mikael P. Backlund ◽  
Alexander R. von Diezmann ◽  
Steffen J. Sahl ◽  
W. E. Moerner
Keyword(s):  
Single Molecule ◽  
Three Dimensional ◽  
Super Resolution ◽  
Orientational Dynamics ◽  
Super Resolution Microscopy

scholarly journals New mechanism of fibronectin fibril assembly revealed by live imaging and super-resolution microscopy

2020 ◽  
Author(s):  
Darshika Tomer ◽  
Sudipto Munshi ◽  
Brianna E. Alexander ◽  
Brenda French ◽  
Pavan Vedula ◽  
...  
Keyword(s):  
Cell Fate ◽  
Three Dimensional ◽  
Super Resolution ◽  
Live Imaging ◽  
Modular Architecture ◽  
Ecm Remodeling ◽  
Linear Arrays ◽  
Cell Fate Decisions ◽  
Super Resolution Microscopy

AbstractThe regulation of cell fate decisions, morphogenesis, and responses to injury are intimately linked to the process of Fn1 fibrillogenesis. Live imaging and super-resolution microscopy revealed that Fn1 fibrils are not continuous. Instead, Fn1 fibrils arise from nanodomains containing multiple Fn1 dimers. As they move toward cell center, Fn1 nanodomains become organized into linear arrays with a spacing of 130 nm between the nanodomains, with little Fn1 in between; Fn1 nanodomain arrays are resistant to deoxycholate treatment demonstrating that these beaded assemblies are indeed mature Fn1 fibrils. FUD, a bacterial peptide that disrupts Fn1 fibrillogenesis, does not disrupt nanodomain formation; instead, it interferes with the organization of nanodomains into arrays. The nanodomain composition of Fn1 fibrils is observed in multiple contexts: in three-dimensional ECM in vivo, on substrata of different composition and stiffness, and is retained in the absence of cells. The modular architecture of Fn1 fibrils bears important implications for mechanisms of ECM remodeling and signal transduction.

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