New Design Method for a Phase Plate in Super-Resolution Fluorescence Microscopy

2014 ◽  
Vol 68 (3) ◽  
pp. 353-361 ◽  
Author(s):  
Nandor Bokor ◽  
Yoshinori Iketaki
Keyword(s):  
Fluorescence Microscopy ◽  
Design Method ◽  
Super Resolution ◽  
Phase Plate

scholarly journals Advanced Static and Dynamic Fluorescence Microscopy Techniques to Investigate Drug Delivery Systems

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 861
Author(s):  
Jacopo Cardellini ◽  
Arianna Balestri ◽  
Costanza Montis ◽  
Debora Berti
Keyword(s):  
Drug Delivery ◽  
Fluorescence Microscopy ◽  
Drug Delivery Systems ◽  
Laser Scanning ◽  
Super Resolution ◽  
Laser Scanning Confocal Microscopy ◽  
Delivery Systems ◽  
Scanning Confocal Microscopy ◽  
Biological Phenomena

In the past decade(s), fluorescence microscopy and laser scanning confocal microscopy (LSCM) have been widely employed to investigate biological and biomimetic systems for pharmaceutical applications, to determine the localization of drugs in tissues or entire organisms or the extent of their cellular uptake (in vitro). However, the diffraction limit of light, which limits the resolution to hundreds of nanometers, has for long time restricted the extent and quality of information and insight achievable through these techniques. The advent of super-resolution microscopic techniques, recognized with the 2014 Nobel prize in Chemistry, revolutionized the field thanks to the possibility to achieve nanometric resolution, i.e., the typical scale length of chemical and biological phenomena. Since then, fluorescence microscopy-related techniques have acquired renewed interest for the scientific community, both from the perspective of instrument/techniques development and from the perspective of the advanced scientific applications. In this contribution we will review the application of these techniques to the field of drug delivery, discussing how the latest advancements of static and dynamic methodologies have tremendously expanded the experimental opportunities for the characterization of drug delivery systems and for the understanding of their behaviour in biologically relevant environments.

Fabrication of optical multilayer for two-color phase plate in super-resolution microscope

2014 ◽  
Vol 85 (7) ◽  
pp. 073701
Author(s):  
Yoshinori Iketaki ◽  
Katsuichi Kitagawa ◽  
Kohjiro Hidaka ◽  
Naoki Kato ◽  
Akira Hirabayashi ◽  
...  
Keyword(s):  
Super Resolution ◽  
Phase Plate

scholarly journals Facile method to stain the bacterial cell surface for super-resolution fluorescence microscopy

The Analyst ◽  
2014 ◽  
Vol 139 (12) ◽  
pp. 3174-3178 ◽  
Author(s):  
Ian L. Gunsolus ◽  
Dehong Hu ◽  
Cosmin Mihai ◽  
Samuel E. Lohse ◽  
Chang-soo Lee ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Surface ◽  
Bacterial Cell ◽  
Super Resolution ◽  
Bacterial Cell Surface

Super-resolution FRET measurements

Nanoscale ◽  
2021 ◽  
Author(s):  
Fernando D Stefani ◽  
Alan M. M. Szalai ◽  
Cecilia Zaza
Keyword(s):  
Energy Transfer ◽  
Fluorescence Microscopy ◽  
Biological Systems ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Super Resolution ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Dynamic Architecture ◽  
Förster Resonance

Super-resolution fluorescence microscopy and Förster Resonance Energy Transfer (FRET) form a well-established family of techniques that has provided unique tools to study the dynamic architecture and functionality of biological systems,...

scholarly journals Motion Analysis of Live Objects by Super-Resolution Fluorescence Microscopy

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Chunyan Yao ◽  
Jianwei Zhang ◽  
Guang Wu ◽  
Houxiang Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Motion Analysis ◽  
Cell Cancer ◽  
Super Resolution ◽  
Biological Engineering ◽  
Related Field ◽  
Cellular Analysis ◽  
Detection And Tracking ◽  
Motion Behavior ◽  
Analysis Of Motion

Motion analysis plays an important role in studing activities or behaviors of live objects in medicine, biotechnology, chemistry, physics, spectroscopy, nanotechnology, enzymology, and biological engineering. This paper briefly reviews the developments in this area mostly in the recent three years, especially for cellular analysis in fluorescence microscopy. The topic has received much attention with the increasing demands in biomedical applications. The tasks of motion analysis include detection and tracking of objects, as well as analysis of motion behavior, living activity, events, motion statistics, and so forth. In the last decades, hundreds of papers have been published in this research topic. They cover a wide area, such as investigation of cell, cancer, virus, sperm, microbe, karyogram, and so forth. These contributions are summarized in this review. Developed methods and practical examples are also introduced. The review is useful to people in the related field for easy referral of the state of the art.

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