scholarly journals Label-free isolation of circulating tumor cells in microfluidic devices: Current research and perspectives

2013 ◽  
Vol 7 (1) ◽  
pp. 011810 ◽  
Author(s):  
Igor Cima ◽  
Chay Wen Yee ◽  
Florina S. Iliescu ◽  
Wai Min Phyo ◽  
Kiat Hon Lim ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Devices ◽  
Label Free

scholarly journals 56P Single circulating tumor cells RNA profiling by label-free enrichment and active single cell selection on an integrated fluidic system

2016 ◽  
Vol 27 ◽  
pp. ix15-ix16
Author(s):  
Y.F. Lee ◽  
N. Ramalingam ◽  
L. Szpankowski ◽  
A. Leyrat ◽  
N.D. Angeles ◽  
...  
Keyword(s):  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cell Selection ◽  
Label Free ◽  
Rna Profiling ◽  
Free Enrichment

scholarly journals Label-free enumeration, collection and downstream cytological and cytogenetic analysis of circulating tumor cells

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Manjima Dhar ◽  
Edward Pao ◽  
Corinne Renier ◽  
Derek E. Go ◽  
James Che ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cytogenetic Analysis ◽  
Label Free

A Chemical Method for Specific Capture of Circulating Tumor Cells Using Label-Free Polyphenol-Functionalized Films

2018 ◽  
Vol 30 (13) ◽  
pp. 4372-4382 ◽  
Author(s):  
Liwei Yang ◽  
He Sun ◽  
Wenning Jiang ◽  
Ting Xu ◽  
Bing Song ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Chemical Method ◽  
Label Free ◽  
Functionalized Films

scholarly journals Perspectives on liquid biopsy for label‐free detection of “circulating tumor cells” through intelligent lab‐on‐chips

View ◽  
2020 ◽  
Vol 1 (3) ◽  
pp. 20200034 ◽  
Author(s):  
Lisa Miccio ◽  
Flora Cimmino ◽  
Ivana Kurelac ◽  
Massimiliano M. Villone ◽  
Vittorio Bianco ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Liquid Biopsy ◽  
Label Free ◽  
Label Free Detection

scholarly journals Nanotechnology-Assisted Isolation and Analysis of Circulating Tumor Cells on Microfluidic Devices

Micromachines ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 774 ◽  
Author(s):  
Jie Cheng ◽  
Yang Liu ◽  
Yang Zhao ◽  
Lina Zhang ◽  
Lingqian Zhang ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Liquid Biopsy ◽  
Cancer Metastasis ◽  
Human Peripheral Blood ◽  
Microfluidic Devices ◽  
Detection Sensitivity ◽  
Cell Capture ◽  
Primary Tumors ◽  
Downstream Analysis

Circulating tumor cells (CTCs), a type of cancer cell that spreads from primary tumors into human peripheral blood and are considered as a new biomarker of cancer liquid biopsy. It provides the direction for understanding the biology of cancer metastasis and progression. Isolation and analysis of CTCs offer the possibility for early cancer detection and dynamic prognosis monitoring. The extremely low quantity and high heterogeneity of CTCs are the major challenges for the application of CTCs in liquid biopsy. There have been significant research endeavors to develop efficient and reliable approaches to CTC isolation and analysis in the past few decades. With the advancement of microfabrication and nanomaterials, a variety of approaches have now emerged for CTC isolation and analysis on microfluidic platforms combined with nanotechnology. These new approaches show advantages in terms of cell capture efficiency, purity, detection sensitivity and specificity. This review focuses on recent progress in the field of nanotechnology-assisted microfluidics for CTC isolation and detection. Firstly, CTC isolation approaches using nanomaterial-based microfluidic devices are summarized and discussed. The different strategies for CTC release from the devices are specifically outlined. In addition, existing nanotechnology-assisted methods for CTC downstream analysis are summarized. Some perspectives are discussed on the challenges of current methods for CTC studies and promising research directions.

scholarly journals Dysmetabolic Circulating Tumor Cells Are Prognostic in Metastatic Breast Cancer

Cancers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1005 ◽  
Author(s):  
Giulia Brisotto ◽  
Eva Biscontin ◽  
Elisabetta Rossi ◽  
Michela Bulfoni ◽  
Aigars Piruska ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Metastatic Breast ◽  
Therapy Response ◽  
Label Free ◽  
Breast Cancer Patients ◽  
Combining Data ◽  
Definition Of

Circulating tumor cells (CTCs) belong to a heterogeneous pool of rare cells, and a unequivocal phenotypic definition of CTC is lacking. Here, we present a definition of metabolically-altered CTC (MBA-CTCs) as CD45-negative cells with an increased extracellular acidification rate, detected with a single-cell droplet microfluidic technique. We tested the prognostic value of MBA-CTCs in 31 metastatic breast cancer patients before starting a new systemic therapy (T0) and 3–4 weeks after (T1), comparing results with a parallel FDA-approved CellSearch (CS) approach. An increased level of MBA-CTCs was associated with: i) a shorter median PFS pre-therapy (123 days vs. 306; p < 0.0001) and during therapy (139 vs. 266 days; p = 0.0009); ii) a worse OS pre-therapy (p = 0.0003, 82% survival vs. 20%) and during therapy (p = 0.0301, 67% survival vs. 38%); iii) good agreement with therapy response (kappa = 0.685). The trend of MBA-CTCs over time (combining data at T0 and T1) added information with respect to separate evaluation of T0 and T1. The combined results of the two assays (MBA and CS) increased stratification accuracy, while correlation between MBA and CS was not significant, suggesting that the two assays are detecting different CTC subsets. In conclusion, this study suggests that MBA allows detection of both EpCAM-negative and EpCAM-positive, viable and label-free CTCs, which provide clinical information apparently equivalent and complementary to CS. A further validation of proposed method and cut-offs is needed in a larger, separate study.

Magnetic particles assisted capture and release of rare circulating tumor cells using wavy-herringbone structured microfluidic devices

Lab on a Chip ◽  
2017 ◽  
Vol 17 (19) ◽  
pp. 3291-3299 ◽  
Author(s):  
Wentao Shi ◽  
Shunqiang Wang ◽  
Ahmad Maarouf ◽  
Christopher G. Uhl ◽  
Ran He ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Magnetic Particles ◽  
Microfluidic Devices ◽  
Rare Tumor ◽  
Capture And Release

Wavy-herringbone structured microfluidic devices promote capture and collection of rare tumor cells assisted by magnetic particles.

scholarly journals Slanted spiral microfluidics for the ultra-fast, label-free isolation of circulating tumor cells

Lab on a Chip ◽  
2014 ◽  
Vol 14 (1) ◽  
pp. 128-137 ◽  
Author(s):  
Majid Ebrahimi Warkiani ◽  
Guofeng Guan ◽  
Khoo Bee Luan ◽  
Wong Cheng Lee ◽  
Ali Asgar S. Bhagat ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Label Free
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