scholarly journals Perspectives on liquid biopsy for label‐free detection of “circulating tumor cells” through intelligent lab‐on‐chips

View ◽  
2020 ◽  
Vol 1 (3) ◽  
pp. 20200034 ◽  
Author(s):  
Lisa Miccio ◽  
Flora Cimmino ◽  
Ivana Kurelac ◽  
Massimiliano M. Villone ◽  
Vittorio Bianco ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Liquid Biopsy ◽  
Label Free ◽  
Label Free Detection

scholarly journals Cover Picture: Perspectives on liquid biopsy for label‐free detection of “circulating tumor cells” through intelligent lab‐on‐chips (View 3/2020)

View ◽  
2020 ◽  
Vol 1 (3) ◽  
Author(s):  
Lisa Miccio ◽  
Flora Cimmino ◽  
Ivana Kurelac ◽  
Massimiliano M. Villone ◽  
Vittorio Bianco ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Liquid Biopsy ◽  
Label Free ◽  
Label Free Detection

Direct Plasmon-Enhanced Electrochemistry for Enabling Ultrasensitive and Label-Free Detection of Circulating Tumor Cells in Blood

2019 ◽  
Vol 91 (7) ◽  
pp. 4413-4420 ◽  
Author(s):  
Shan-Shan Wang ◽  
Xiao-Ping Zhao ◽  
Fei-Fei Liu ◽  
Muhammad Rizwan Younis ◽  
Xing-Hua Xia ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Label Free ◽  
Label Free Detection

scholarly journals Development of a Microfluidic-Based Optical Sensing Device for Label-Free Detection of Circulating Tumor Cells (CTCs) Through Their Lactic Acid Metabolism

Sensors ◽  
2015 ◽  
Vol 15 (3) ◽  
pp. 6789-6806 ◽  
Author(s):  
Tzu-Keng Chiu ◽  
Kin-Fong Lei ◽  
Chia-Hsun Hsieh ◽  
Hung-Bo Hsiao ◽  
Hung-Ming Wang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Acid Metabolism ◽  
Optical Sensing ◽  
Label Free ◽  
Label Free Detection ◽  
Lactic Acid Metabolism

scholarly journals VTX‐1 Liquid Biopsy System for Fully‐Automated and Label‐Free Isolation of Circulating Tumor Cells with Automated Enumeration by BioView Platform

2018 ◽  
Vol 93 (12) ◽  
pp. 1240-1245 ◽  
Author(s):  
Elodie Sollier‐Christen ◽  
Corinne Renier ◽  
Tal Kaplan ◽  
Elad Kfir ◽  
Steve C. Crouse
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Liquid Biopsy ◽  
Label Free

scholarly journals Label-free detection of rare circulating tumor cells by image analysis and machine learning

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shen Wang ◽  
Yuyuan Zhou ◽  
Xiaochen Qin ◽  
Suresh Nair ◽  
Xiaolei Huang ◽  
...  
Keyword(s):  
Machine Learning ◽  
Image Analysis ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Label Free ◽  
Label Free Detection

scholarly journals High Clinical Value of Liquid Biopsy to Detect Circulating Tumor Cells and Tumor Exosomes in Pancreatic Ductal Adenocarcinoma Patients Eligible for Up-Front Surgery

Cancers ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1656 ◽  
Author(s):  
Etienne Buscail ◽  
Catherine Alix-Panabières ◽  
Pascaline Quincy ◽  
Thomas Cauvin ◽  
Alexandre Chauvet ◽  
...  
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Liquid Biopsy ◽  
Neoadjuvant Treatment ◽  
Digital Pcr ◽  
Portal Blood ◽  
Ductal Adenocarcinoma ◽  
Clinical Value ◽  
Liquid Biopsies

Purpose: Expediting the diagnosis of pancreatic ductal adenocarcinoma (PDAC) would benefit care management, especially for the start of treatments requiring histological evidence. This study evaluated the combined diagnostic performance of circulating biomarkers obtained by peripheral and portal blood liquid biopsy in patients with resectable PDAC. Experimental design: Liquid biopsies were performed in a prospective translational clinical trial (PANC-CTC #NCT03032913) including 22 patients with resectable PDAC and 28 noncancer controls from February to November 2017. Circulating tumor cells (CTCs) were detected using the CellSearch® method or after RosetteSep® enrichment combined with CRISPR/Cas9-improved KRAS mutant alleles quantification by droplet digital PCR. CD63 bead-coupled Glypican-1 (GPC1)-positive exosomes were quantified by flow cytometry. Results: Liquid biopsies were positive in 7/22 (32%), 13/22 (59%), and 14/22 (64%) patients with CellSearch® or RosetteSep®-based CTC detection or GPC1-positive exosomes, respectively, in peripheral and/or portal blood. Liquid biopsy performance was improved in portal blood only with CellSearch®, reaching 45% of PDAC identification (5/11) versus 10% (2/22) in peripheral blood. Importantly, combining CTC and GPC1-positive-exosome detection displayed 100% of sensitivity and 80% of specificity, with a negative predictive value of 100%. High levels of GPC1+-exosomes and/or CTC presence were significantly correlated with progression-free survival and with overall survival when CTC clusters were found. Conclusion: This study is the first to evaluate combined CTC and exosome detection to diagnose resectable pancreatic cancers. Liquid biopsy combining several biomarkers could provide a rapid, reliable, noninvasive decision-making tool in early, potentially curable pancreatic cancer. Moreover, the prognostic value could select patients eligible for neoadjuvant treatment before surgery. This exploratory study deserves further validation.

scholarly journals 56P Single circulating tumor cells RNA profiling by label-free enrichment and active single cell selection on an integrated fluidic system

2016 ◽  
Vol 27 ◽  
pp. ix15-ix16
Author(s):  
Y.F. Lee ◽  
N. Ramalingam ◽  
L. Szpankowski ◽  
A. Leyrat ◽  
N.D. Angeles ◽  
...  
Keyword(s):  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cell Selection ◽  
Label Free ◽  
Rna Profiling ◽  
Free Enrichment
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