scholarly journals Synchronous optical and electrical detection of biomolecules traversing through solid-state nanopores

2010 ◽  
Vol 81 (1) ◽  
pp. 014301 ◽  
Author(s):  
Gautam V. Soni ◽  
Alon Singer ◽  
Zhiliang Yu ◽  
Yingjie Sun ◽  
Ben McNally ◽  
...  
Keyword(s):  
Solid State ◽  
Electrical Detection ◽  
Detection Of Biomolecules

scholarly journals An all-solid-state heterojunction oxide transistor for the rapid detection of biomolecules and SARS-CoV-2 spike S1 protein

2021 ◽  
Author(s):  
Yen-Hung Lin ◽  
Yang Han ◽  
Abhinav Sharma ◽  
Wejdan S. AlGhamdi ◽  
Chien-Hao Liu ◽  
...  
Keyword(s):  
Solid State ◽  
Real Time ◽  
High Sensitivity ◽  
Cost Effective ◽  
High Electron ◽  
Real Time Detection ◽  
Bioanalytical Applications ◽  
Detection Of Biomolecules ◽  
Dimensional Electron Gas ◽  
Sensitivity Specificity

AbstractSolid-state transistor sensors that can detect biomolecules in real time are highly attractive for emerging bioanalytical applications. However, combining cost-effective manufacturing with high sensitivity, specificity and fast sensing response, remains challenging. Here we develop low-temperature solution-processed In2O3/ZnO heterojunction transistors featuring a geometrically engineered tri-channel architecture for rapid real-time detection of different biomolecules. The sensor combines a high electron mobility channel, attributed to the quasi-two-dimensional electron gas (q2DEG) at the buried In2O3/ZnO heterointerface, in close proximity to a sensing surface featuring tethered analyte receptors. The unusual tri-channel design enables strong coupling between the buried q2DEG and the minute electronic perturbations occurring during receptor-analyte interactions allowing for robust, real-time detection of biomolecules down to attomolar (aM) concentrations. By functionalizing the tri-channel surface with SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) antibody receptors, we demonstrate real-time detection of the SARS-CoV-2 spike S1 protein down to attomolar concentrations in under two minutes.

scholarly journals Microfluidic Systems Applied in Solid-State Nanopore Sensors

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 332 ◽  
Author(s):  
Jiye Fu ◽  
Linlin Wu ◽  
Yi Qiao ◽  
Jing Tu ◽  
Zuhong Lu
Keyword(s):  
Experimental Study ◽  
Solid State ◽  
Microfluidic System ◽  
Detection Methods ◽  
Electrical Detection ◽  
Microfluidic Systems ◽  
Novel Device ◽  
Device Structures ◽  
Nanopore Sensors

Microfluidic system, as a kind of miniature integrated operating platform, has been applied to solid-state nanopore sensors after many years of experimental study. In the process of introducing microfluidic into solid-state nanopore sensors, many novel device structures are designed due to the abundance of analytes and the diversity of detection methods. Here we review the fundamental setup of nanopore-based microfluidic systems and the developments and advancements that have been taking place in the field. The microfluidic systems with a multichannel strategy to elevate the throughput and efficiency of nanopore sensors are then presented. Multifunctional detection represented by optical-electrical detection, which is realized by microfluidic integration, is also described. A high integration microfluidic system with nanopore is further discussed, which shows the prototype of commercialization.

Solid-state nanopores for biosensing with submolecular resolution

2012 ◽  
Vol 40 (4) ◽  
pp. 624-628 ◽  
Author(s):  
Azadeh Bahrami ◽  
Fatma Doğan ◽  
Deanpen Japrung ◽  
Tim Albrecht
Keyword(s):  
Solid State ◽  
Dna Sequencing ◽  
Single Molecule ◽  
Dna Interactions ◽  
Patch Clamp Technique ◽  
New Class ◽  
Protein Dna Interactions ◽  
Transmembrane Pores ◽  
Recent Developments ◽  
Detection Of Biomolecules

Biological cell membranes contain various types of ion channels and transmembrane pores in the 1–100 nm range, which are vital for cellular function. Individual channels can be probed electrically, as demonstrated by Neher and Sakmann in 1976 using the patch-clamp technique [Neher and Sakmann (1976) Nature 260, 799–802]. Since the 1990s, this work has inspired the use of protein or solid-state nanopores as inexpensive and ultrafast sensors for the detection of biomolecules, including DNA, RNA and proteins, but with particular focus on DNA sequencing. Solid-state nanopores in particular have the advantage that the pore size can be tailored to the analyte in question and that they can be modified using semi-conductor processing technology. This establishes solid-state nanopores as a new class of single-molecule biosensor devices, in some cases with submolecular resolution. In the present review, we discuss a few of the most important recent developments in this field and how they might be applied to studying protein–protein and protein–DNA interactions or in the context of ultra-fast DNA sequencing.

Rapid, multiplexed detection of biomolecules using electrically distinct hydrogel beads

Lab on a Chip ◽  
2020 ◽  
Vol 20 (13) ◽  
pp. 2274-2283
Author(s):  
Thomas W. Cowell ◽  
Enrique Valera ◽  
Aaron Jankelow ◽  
Joonhyuck Park ◽  
Alex W. Schrader ◽  
...  
Keyword(s):  
Electrical Detection ◽  
Multiplexed Detection ◽  
Hydrogel Beads ◽  
Coulter Counting ◽  
Detection Of Biomolecules

Electrically distinct microparticles enable rapid, versatile, and multiplexed electrical detection of biomolecules using Coulter counting-based detection.

Controlled gating and electrical detection of single 50S ribosomal subunits through a solid-state nanopore in a microfluidic chip

2011 ◽  
Vol 29 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Mikhail I. Rudenko ◽  
Matthew R. Holmes ◽  
Dmitri N. Ermolenko ◽  
Evan J. Lunt ◽  
Sarah Gerhardt ◽  
...  
Keyword(s):  
Solid State ◽  
Microfluidic Chip ◽  
Electrical Detection ◽  
Ribosomal Subunits
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