scholarly journals A transparent cell-culture microchamber with a variably controlled concentration gradient generator and flow field rectifier

2008 ◽  
Vol 2 (2) ◽  
pp. 024105 ◽  
Author(s):  
Ji-Yen Cheng ◽  
Meng-Hua Yen ◽  
Ching-Te Kuo ◽  
Tai-Horng Young
Keyword(s):  
Cell Culture ◽  
Flow Field ◽  
Concentration Gradient ◽  
Gradient Generator ◽  
Transparent Cell

scholarly journals A Microfluidic Spheroid Culture Device with a Concentration Gradient Generator for High-Throughput Screening of Drug Efficacy

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3355 ◽  
Author(s):  
Wanyoung Lim ◽  
Sungsu Park
Keyword(s):  
High Throughput ◽  
Concentration Gradient ◽  
High Throughput Screening ◽  
3D Cell Culture ◽  
Drug Efficacy ◽  
Cancer Drug ◽  
Spheroid Culture ◽  
Gradient Generator ◽  
Micro Channels

Three-dimensional (3D) cell culture is considered more clinically relevant in mimicking the structural and physiological conditions of tumors in vivo compared to two-dimensional cell cultures. In recent years, high-throughput screening (HTS) in 3D cell arrays has been extensively used for drug discovery because of its usability and applicability. Herein, we developed a microfluidic spheroid culture device (μFSCD) with a concentration gradient generator (CGG) that enabled cells to form spheroids and grow in the presence of cancer drug gradients. The device is composed of concave microwells with several serpentine micro-channels which generate a concentration gradient. Once the colon cancer cells (HCT116) formed a single spheroid (approximately 120 μm in diameter) in each microwell, spheroids were perfused in the presence of the cancer drug gradient irinotecan for three days. The number of spheroids, roundness, and cell viability, were inversely proportional to the drug concentration. These results suggest that the μFSCD with a CGG has the potential to become an HTS platform for screening the efficacy of cancer drugs.

A novel microfluidic platform with stable concentration gradient for on chip cell culture and screening assays

Lab on a Chip ◽  
2013 ◽  
Vol 13 (18) ◽  
pp. 3714 ◽  
Author(s):  
Bi-Yi Xu ◽  
Shan-Wen Hu ◽  
Guang-Sheng Qian ◽  
Jing-Juan Xu ◽  
Hong-Yuan Chen
Keyword(s):  
Cell Culture ◽  
Concentration Gradient ◽  
Microfluidic Platform ◽  
Screening Assays ◽  
On Chip

A radial microfluidic concentration gradient generator with high-density channels for cell apoptosis assay

Lab on a Chip ◽  
2011 ◽  
Vol 11 (19) ◽  
pp. 3305 ◽  
Author(s):  
Chun-Guang Yang ◽  
Ying-Fan Wu ◽  
Zhang-Run Xu ◽  
Jian-Hua Wang
Keyword(s):  
Concentration Gradient ◽  
Cell Apoptosis ◽  
High Density ◽  
Gradient Generator ◽  
Apoptosis Assay

Research on the Pollution Diffusion Regularity near Sewage Outlet Areas in Yuncheng Reach of the Fen River

2011 ◽  
Vol 383-390 ◽  
pp. 2430-2436
Author(s):  
Jian Hua Hou ◽  
Min Quan Feng ◽  
Xiao Peng Xing ◽  
Zhen Hua Hou
Keyword(s):  
Water Quality ◽  
Flow Field ◽  
Water Depth ◽  
Concentration Gradient ◽  
River Flow ◽  
Measured Data ◽  
Quality Model

The purpose of this paper is to find the pollution diffusion regularity near sewage outlet area of Yuncheng reach of the Fen River. A 2-D water hydrodynamic and quality model was used to simulate flow field, the water quality and contamination dispersion. The parameters of the model were calibrated with measured data of the water depth, flow and water quality in Yuncheng reach of the Fen River. According to the simulated result, the total area of pollution belt with 19 sewage outlets is 8.89km2 in normal year. And 3.89% of the reach has a worse water quality than V class in standard. The percentage of V and Ⅳ Class of water is 69.17% and 26.94%.In dry year, the total area of pollution belt with 19 sewage outlets is 8.89km2.The percentage of inferior V, V and Ⅳ Class of water is 27.80%, 69.46% and 2.74%. It was shown by the simulated results that the concentration gradient decreases with increasing distance to the outlets and the dilution and dispersion of pollutants was enhanced by a greater river flow.

Multichannel Synchronous Hydrodynamic Gating Coupling with Concentration Gradient Generator for High-Throughput Probing Dynamic Signaling of Single Cells

2020 ◽  
Vol 92 (17) ◽  
pp. 12062-12070
Author(s):  
Yiran Guo ◽  
Zhaolong Gao ◽  
Yinan Liu ◽  
Shunji Li ◽  
Jinchi Zhu ◽  
...  
Keyword(s):  
High Throughput ◽  
Concentration Gradient ◽  
Single Cells ◽  
Gradient Generator

scholarly journals A valve-free 2D concentration gradient generator

RSC Advances ◽  
2017 ◽  
Vol 7 (45) ◽  
pp. 27833-27839 ◽  
Author(s):  
Jingxuan Tian ◽  
Yibo Gao ◽  
Bingpu Zhou ◽  
Wenbin Cao ◽  
Xiaoxiao Wu ◽  
...  
Keyword(s):  
Concentration Gradient ◽  
Flow Path ◽  
3D Flow ◽  
Gradient Generator

Our designed chip with a criss-cross 3D flow path realizes a valve-free 2D concentration gradient generator.

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