scholarly journals A valve-free 2D concentration gradient generator

RSC Advances ◽  
2017 ◽  
Vol 7 (45) ◽  
pp. 27833-27839 ◽  
Author(s):  
Jingxuan Tian ◽  
Yibo Gao ◽  
Bingpu Zhou ◽  
Wenbin Cao ◽  
Xiaoxiao Wu ◽  
...  
Keyword(s):  
Concentration Gradient ◽  
Flow Path ◽  
3D Flow ◽  
Gradient Generator

Our designed chip with a criss-cross 3D flow path realizes a valve-free 2D concentration gradient generator.

scholarly journals A Microfluidic Spheroid Culture Device with a Concentration Gradient Generator for High-Throughput Screening of Drug Efficacy

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3355 ◽  
Author(s):  
Wanyoung Lim ◽  
Sungsu Park
Keyword(s):  
High Throughput ◽  
Concentration Gradient ◽  
High Throughput Screening ◽  
3D Cell Culture ◽  
Drug Efficacy ◽  
Cancer Drug ◽  
Spheroid Culture ◽  
Gradient Generator ◽  
Micro Channels

Three-dimensional (3D) cell culture is considered more clinically relevant in mimicking the structural and physiological conditions of tumors in vivo compared to two-dimensional cell cultures. In recent years, high-throughput screening (HTS) in 3D cell arrays has been extensively used for drug discovery because of its usability and applicability. Herein, we developed a microfluidic spheroid culture device (μFSCD) with a concentration gradient generator (CGG) that enabled cells to form spheroids and grow in the presence of cancer drug gradients. The device is composed of concave microwells with several serpentine micro-channels which generate a concentration gradient. Once the colon cancer cells (HCT116) formed a single spheroid (approximately 120 μm in diameter) in each microwell, spheroids were perfused in the presence of the cancer drug gradient irinotecan for three days. The number of spheroids, roundness, and cell viability, were inversely proportional to the drug concentration. These results suggest that the μFSCD with a CGG has the potential to become an HTS platform for screening the efficacy of cancer drugs.

A radial microfluidic concentration gradient generator with high-density channels for cell apoptosis assay

Lab on a Chip ◽  
2011 ◽  
Vol 11 (19) ◽  
pp. 3305 ◽  
Author(s):  
Chun-Guang Yang ◽  
Ying-Fan Wu ◽  
Zhang-Run Xu ◽  
Jian-Hua Wang
Keyword(s):  
Concentration Gradient ◽  
Cell Apoptosis ◽  
High Density ◽  
Gradient Generator ◽  
Apoptosis Assay

Multichannel Synchronous Hydrodynamic Gating Coupling with Concentration Gradient Generator for High-Throughput Probing Dynamic Signaling of Single Cells

2020 ◽  
Vol 92 (17) ◽  
pp. 12062-12070
Author(s):  
Yiran Guo ◽  
Zhaolong Gao ◽  
Yinan Liu ◽  
Shunji Li ◽  
Jinchi Zhu ◽  
...  
Keyword(s):  
High Throughput ◽  
Concentration Gradient ◽  
Single Cells ◽  
Gradient Generator

Concentration gradient generator for H460 lung cancer cell sensitivity to resist the cytotoxic action of curcumin in microenvironmental pH conditions

RSC Advances ◽  
2016 ◽  
Vol 6 (109) ◽  
pp. 107310-107316
Author(s):  
Chunfei Hu ◽  
Yu-Sheng Lin ◽  
Hongmei Chen ◽  
Jingjing Liu ◽  
Fuqiang Nie
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Concentration Gradient ◽  
Cytotoxic Action ◽  
Lung Cancer Cell ◽  
Cell Sensitivity ◽  
Gradient Generator ◽  
Microenvironmental Ph ◽  
Ph Conditions

We proposed and demonstrated a concentration gradient generator (CGG) to resist H460 lung cancer cells using curcumin in microenvironmental pH conditions.

scholarly journals High-glucose 3D INS-1 cell model combined with a microfluidic circular concentration gradient generator for high throughput screening of drugs against type 2 diabetes

RSC Advances ◽  
2018 ◽  
Vol 8 (45) ◽  
pp. 25409-25416 ◽  
Author(s):  
Yong Luo ◽  
Xiuli Zhang ◽  
Yujiao Li ◽  
Jiu Deng ◽  
Xiaorui Li ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Pharmaceutical Industry ◽  
High Throughput ◽  
Concentration Gradient ◽  
High Glucose ◽  
High Throughput Screening ◽  
Cell Model ◽  
Gradient Generator

In vitro models for screening of drugs against type 2 diabetes are crucial for the pharmaceutical industry.

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