A novel microfluidic platform with stable concentration gradient for on chip cell culture and screening assays

Lab on a Chip ◽  
2013 ◽  
Vol 13 (18) ◽  
pp. 3714 ◽  
Author(s):  
Bi-Yi Xu ◽  
Shan-Wen Hu ◽  
Guang-Sheng Qian ◽  
Jing-Juan Xu ◽  
Hong-Yuan Chen
Keyword(s):  
Cell Culture ◽  
Concentration Gradient ◽  
Microfluidic Platform ◽  
Screening Assays ◽  
On Chip

scholarly journals On-chip MIC by Combining Concentration Gradient Generator and Flanged Chamber Arrays

Micromachines ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 207 ◽  
Author(s):  
Xiao-Yan Zhang ◽  
Zhe-Yu Li ◽  
Kose Ueno ◽  
Hiroaki Misawa ◽  
Nan-Qi Ren ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Concentration Gradient ◽  
Microfluidic System ◽  
Honeycomb Structure ◽  
Inhibition Test ◽  
Microfluidic Platform ◽  
Initial Concentration ◽  
Bacteria Growth ◽  
Skilled Operator ◽  
On Chip

Minimum inhibition concentration (MIC) of antibiotic is an effective value to ascertain the agent and minimum dosage of inhibiting bacterial growth. However, current techniques to determine MIC are labor intensive and time-consuming, and require skilled operator and high initial concentration of bacteria. To simplify the operation and reduce the time of inhibition test, we developed a microfluidic system, containing a concentration generator and sub-micro-liter chambers, for rapid bacterial growth and inhibition test. To improve the mixing effect, a micropillar array in honeycomb-structure channels is designed, so the steady concentration gradient of amoxicillin can be generated. The flanged chambers are used to culture bacteria under the condition of continuous flow and the medium of chambers is refreshed constantly, which could supply the sufficient nutrient for bacteria growth and take away the metabolite. Based on the microfluidic platform, the bacterial growth with antibiotic inhibition on chip can be quantitatively measured and MIC can be obtained within six hours using low initial concentration of bacteria. Overall, this microfluidic platform has the potential to provide rapidness and effectiveness to screen bacteria and determine MIC of corresponding antibiotics in clinical therapies.

An automated centrifugal microfluidic assay for whole blood fractionation and isolation of multiple cell populations using an aqueous two-phase system

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Byeong-Ui Moon ◽  
Liviu Clime ◽  
Daniel Brassard ◽  
Alex Boutin ◽  
Jamal Daoud ◽  
...  
Keyword(s):  
Human Blood ◽  
Whole Blood ◽  
Cell Populations ◽  
Phase System ◽  
Two Phase ◽  
Microfluidic Platform ◽  
Aqueous Two Phase System ◽  
Separation Layers ◽  
Multiple Cell ◽  
On Chip

This paper describes an advanced on-chip whole human blood fractionation and cell isolation process combining an aqueous two-phase system to create complex separation layers with a centrifugal microfluidic platform to control and automate the assay.

Microfluidic Gas Sensing with Living Microbial Cells Confined in a Microaquarium

2013 ◽  
Vol 543 ◽  
pp. 431-434 ◽  
Author(s):  
Kazunari Ozasa ◽  
Jee Soo Lee ◽  
Simon Song ◽  
Masahiko Hara ◽  
Mizuo Maeda
Keyword(s):  
Real Time ◽  
Concentration Gradient ◽  
Gas Sensing ◽  
Bacterial Chemotaxis ◽  
Optical Microscope ◽  
Sensor Chip ◽  
Microfluidic Channels ◽  
Microbial Cells ◽  
Gas Molecules ◽  
On Chip

We investigated on-chip cytotoxicity gas sensing using the bacterial chemotaxis of Euglena confined in a microaquarium. The sensor chip made from PDMS had one microaquarium and two microfluidic channels passing aside of the microaquarium. The chemotactic microbial cells were confined in the microaquarium, whereas two gases (one sample and one reference) flowed in the two isolated microchannels. Gas molecules move from the microchannels into the microaquarium by permeation through porous PDMS wall, and dissolve into the water in the microaquarium, where Euglena cells are swimming. The chemotactic movements of Euglena were observed with an optical microscope and measured as traces in real time. By injecting CO2 and air into each microchannel separately, the Euglena cells in the microaquarium moved to air side, escaping from CO2. This observation showed that the concentration gradient of CO2 was produced in the water in the microaquarium. The CO2-avoiding movement of Euglena was increased largely at a CO2 concentration of 40%, and then moderately increased above 60%. Some Euglena cells stopped swimming at the air side of the microaquarium and remained there even after CO2 has been removed, which can be used as the indicator of CO2 history.

scholarly journals Droplet-assisted electrospray phase separation using an integrated silicon microfluidic platform

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Yan Zhang ◽  
Sungho Kim ◽  
Weihua Shi ◽  
Yaoyao Zhao ◽  
Insu Park ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Phase Separation ◽  
Electrospray Ionization ◽  
Mass Spectrometry Analysis ◽  
Microfluidic Channels ◽  
Droplet Generator ◽  
Microfluidic Platform ◽  
Spectrometry Analysis ◽  
Temporal Phase ◽  
On Chip

We report on a silicon microfluidic platform that enables integration of transparent μm-scale microfluidic channels, an on-chip pL-volume droplet generator, and a nano-electrospray ionization emitter that enables spatial and temporal phase separation for mass spectrometry analysis.

scholarly journals Clarifying intact 3D tissues on a microfluidic chip for high-throughput structural analysis

2016 ◽  
Vol 113 (52) ◽  
pp. 14915-14920 ◽  
Author(s):  
Yih Yang Chen ◽  
Pamuditha N. Silva ◽  
Abdullah Muhammad Syed ◽  
Shrey Sindhwani ◽  
Jonathan V. Rocheleau ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Three Dimensional ◽  
Microfluidic System ◽  
High Throughput Drug Screening ◽  
Image Analysis Algorithm ◽  
Screening Assays ◽  
On Chip ◽  
Tissue Models

On-chip imaging of intact three-dimensional tissues within microfluidic devices is fundamentally hindered by intratissue optical scattering, which impedes their use as tissue models for high-throughput screening assays. Here, we engineered a microfluidic system that preserves and converts tissues into optically transparent structures in less than 1 d, which is 20× faster than current passive clearing approaches. Accelerated clearing was achieved because the microfluidic system enhanced the exchange of interstitial fluids by 567-fold, which increased the rate of removal of optically scattering lipid molecules from the cross-linked tissue. Our enhanced clearing process allowed us to fluorescently image and map the segregation and compartmentalization of different cells during the formation of tumor spheroids, and to track the degradation of vasculature over time within extracted murine pancreatic islets in static culture, which may have implications on the efficacy of beta-cell transplantation treatments for type 1 diabetes. We further developed an image analysis algorithm that automates the analysis of the vasculature connectivity, volume, and cellular spatial distribution of the intact tissue. Our technique allows whole tissue analysis in microfluidic systems, and has implications in the development of organ-on-a-chip systems, high-throughput drug screening devices, and in regenerative medicine.

A microfluidic platform utilizing anchored water-in-oil-in-water double emulsions to create a niche for analyzing single non-adherent cells

Lab on a Chip ◽  
2019 ◽  
Vol 19 (3) ◽  
pp. 422-431 ◽  
Author(s):  
Bo Cai ◽  
Tian-Tian Ji ◽  
Ning Wang ◽  
Xin-Bo Li ◽  
Rong-Xiang He ◽  
...  
Keyword(s):  
Adherent Cells ◽  
Microfluidic Platform ◽  
Double Emulsions ◽  
Oil In Water ◽  
On Chip

Water-in-oil-in-water double emulsions (W/O/W DEs) are generated to encapsulate non-adherent cells and anchored in an array on-chip for in situ assays.

scholarly journals Editorial for the Special Issue on Organs-on-Chips

Micromachines ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 369 ◽  
Author(s):  
Yu-suke Torisawa ◽  
Yi-Chung Tung
Keyword(s):  
Cell Culture ◽  
Special Issue ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Recent Advances ◽  
Functional Units ◽  
On Chip ◽  
Microsystems Technology

Recent advances in microsystems technology and cell culture techniques have led to the development of organ-on-chip microdevices to model functional units of organs [...]

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