Viscosity measurements on micron-size scale using optical tweezers

2005 ◽  
Vol 76 (11) ◽  
pp. 115105 ◽  
Author(s):  
G. Pesce ◽  
A. Sasso ◽  
S. Fusco
Keyword(s):  
Optical Tweezers ◽  
Size Scale ◽  
Viscosity Measurements ◽  
Micron Size

scholarly journals Multipoint viscosity measurements in microfluidic channels using optical tweezers

Lab on a Chip ◽  
2009 ◽  
Vol 9 (14) ◽  
pp. 2059 ◽  
Author(s):  
Stephen Keen ◽  
Alison Yao ◽  
Jonathan Leach ◽  
Roberto Di Leonardo ◽  
Chris Saunter ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Microfluidic Channels ◽  
Viscosity Measurements

scholarly journals Micro-Dumbbells—A Versatile Tool for Optical Tweezers

2018 ◽  
Author(s):  
Weronika Lamperska ◽  
Sławomir Drobczyński ◽  
Michał Nawrot ◽  
Piotr Wasylczyk ◽  
Jan Masajada
Keyword(s):  
Laser Beam ◽  
Optical Tweezers ◽  
Optical Trapping ◽  
Viscosity Measurements ◽  
Two Photon ◽  
Vibration Detection ◽  
Holographic Optical Tweezers ◽  
Versatile Tool ◽  
Micro Tool

Manipulation of micro- and nano-sized objects with optical tweezers is a well established, albeit still evolving technique. While many objects can be trapped directly with focused laser beam(s), for some applications indirect manipulation with tweezers-operated tools is preferred. We introduce a simple, versatile micro-tool operated with holographic optical tweezers. The 40 µm long dumbbell-shaped tool, fabricated with two-photon laser 3D photolithography has two beads for efficient optical trapping and a probing spike on one end. We demonstrate fluids viscosity measurements and vibration detection as examples of possible applications.

Microfluidic production of degradable thermoresponsive poly(N-isopropylacrylamide)-based microgels

Soft Matter ◽  
2017 ◽  
Vol 13 (47) ◽  
pp. 9060-9070 ◽  
Author(s):  
Daryl Sivakumaran ◽  
Eva Mueller ◽  
Todd Hoare
Keyword(s):  
Size Scale ◽  
Precursor Polymers ◽  
Micron Size ◽  
On Chip

Highly monodisperse and hydrolytically degradable thermoresponsive microgels on the tens-to-hundreds of micron size scale have been fabricated based on simultaneous on-chip mixing and emulsification of aldehyde and hydrazide-functionalized poly(N-isopropylacrylamide) precursor polymers.

scholarly journals Trapping sub-micron Size Particles in Holographic Optical Tweezers

2014 ◽  
Vol 534 ◽  
pp. 012059 ◽  
Author(s):  
G Dwivedi ◽  
A Gupta ◽  
M Shukla ◽  
S Kanaujia ◽  
S Yede ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Holographic Optical Tweezers ◽  
Micron Size

scholarly journals Lamin B distribution and association with peripheral chromatin revealed by optical sectioning and electron microscopy tomography.

1993 ◽  
Vol 123 (6) ◽  
pp. 1671-1685 ◽  
Author(s):  
A S Belmont ◽  
Y Zhai ◽  
A Thilenius
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Large Scale ◽  
Light And Electron Microscopy ◽  
Optical Sectioning ◽  
Nuclear Pores ◽  
Immunogold Staining ◽  
Lamin B ◽  
Size Scale ◽  
Micron Size

We have used a combination of immunogold staining, optical sectioning light microscopy, intermediate voltage electron microscopy, and EM tomography to examine the distribution of lamin B over the nuclear envelope of CHO cells. Apparent inconsistencies between previously published light and electron microscopy studies of nuclear lamin staining were resolved. At light microscopy resolution, an apparent open fibrillar network is visualized. Colocalization of lamin B and nuclear pores demonstrates that these apparent fibrils, separated by roughly 0.5 micron, are anti-correlated with the surface distribution of nuclear pores; pore clusters lie between or adjacent to regions of heavy lamin B staining. Examination at higher, EM resolution reveals that this apparent lamin B network does not correspond to an actual network of widely spaced, discrete bundles of lamin filaments. Rather it reflects a quantitative variation in lamin staining over a roughly 0.5-micron size scale, superimposed on a more continuous but still complex distribution of lamin filaments, spatially heterogeneous on a 0.1-0.2-micron size scale. Interestingly, lamin B staining at this higher resolution is highly correlated to the underlying chromatin distribution. Heavy concentrations of lamin B directly "cap" the surface of envelope associated, large-scale chromatin domains.

Defining the trapping limits of holographical optical tweezers

2004 ◽  
Vol 51 (3) ◽  
pp. 409-414 ◽  
Author(s):  
P. Jordan ◽  
J. Leach ◽  
M. J. Padgett ◽  
J. Cooper ◽  
G. Sinclair
Keyword(s):  
Optical Tweezers
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