Viscosity measurements on micron-size scale using optical tweezers

2005 ◽  
Vol 76 (11) ◽  
pp. 115105 ◽  
Author(s):  
G. Pesce ◽  
A. Sasso ◽  
S. Fusco
Lab on a Chip ◽  
2009 ◽  
Vol 9 (14) ◽  
pp. 2059 ◽  
Author(s):  
Stephen Keen ◽  
Alison Yao ◽  
Jonathan Leach ◽  
Roberto Di Leonardo ◽  
Chris Saunter ◽  
...  

Author(s):  
Weronika Lamperska ◽  
Sławomir Drobczyński ◽  
Michał Nawrot ◽  
Piotr Wasylczyk ◽  
Jan Masajada

Manipulation of micro- and nano-sized objects with optical tweezers is a well established, albeit still evolving technique. While many objects can be trapped directly with focused laser beam(s), for some applications indirect manipulation with tweezers-operated tools is preferred. We introduce a simple, versatile micro-tool operated with holographic optical tweezers. The 40 µm long dumbbell-shaped tool, fabricated with two-photon laser 3D photolithography has two beads for efficient optical trapping and a probing spike on one end. We demonstrate fluids viscosity measurements and vibration detection as examples of possible applications.


Soft Matter ◽  
2017 ◽  
Vol 13 (47) ◽  
pp. 9060-9070 ◽  
Author(s):  
Daryl Sivakumaran ◽  
Eva Mueller ◽  
Todd Hoare

Highly monodisperse and hydrolytically degradable thermoresponsive microgels on the tens-to-hundreds of micron size scale have been fabricated based on simultaneous on-chip mixing and emulsification of aldehyde and hydrazide-functionalized poly(N-isopropylacrylamide) precursor polymers.


2014 ◽  
Vol 534 ◽  
pp. 012059 ◽  
Author(s):  
G Dwivedi ◽  
A Gupta ◽  
M Shukla ◽  
S Kanaujia ◽  
S Yede ◽  
...  

1993 ◽  
Vol 123 (6) ◽  
pp. 1671-1685 ◽  
Author(s):  
A S Belmont ◽  
Y Zhai ◽  
A Thilenius

We have used a combination of immunogold staining, optical sectioning light microscopy, intermediate voltage electron microscopy, and EM tomography to examine the distribution of lamin B over the nuclear envelope of CHO cells. Apparent inconsistencies between previously published light and electron microscopy studies of nuclear lamin staining were resolved. At light microscopy resolution, an apparent open fibrillar network is visualized. Colocalization of lamin B and nuclear pores demonstrates that these apparent fibrils, separated by roughly 0.5 micron, are anti-correlated with the surface distribution of nuclear pores; pore clusters lie between or adjacent to regions of heavy lamin B staining. Examination at higher, EM resolution reveals that this apparent lamin B network does not correspond to an actual network of widely spaced, discrete bundles of lamin filaments. Rather it reflects a quantitative variation in lamin staining over a roughly 0.5-micron size scale, superimposed on a more continuous but still complex distribution of lamin filaments, spatially heterogeneous on a 0.1-0.2-micron size scale. Interestingly, lamin B staining at this higher resolution is highly correlated to the underlying chromatin distribution. Heavy concentrations of lamin B directly "cap" the surface of envelope associated, large-scale chromatin domains.


2004 ◽  
Vol 51 (3) ◽  
pp. 409-414 ◽  
Author(s):  
P. Jordan ◽  
J. Leach ◽  
M. J. Padgett ◽  
J. Cooper ◽  
G. Sinclair
Keyword(s):  

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