Microfluidic production of degradable thermoresponsive poly(N-isopropylacrylamide)-based microgels

Soft Matter ◽  
2017 ◽  
Vol 13 (47) ◽  
pp. 9060-9070 ◽  
Author(s):  
Daryl Sivakumaran ◽  
Eva Mueller ◽  
Todd Hoare
Keyword(s):  
Size Scale ◽  
Precursor Polymers ◽  
Micron Size ◽  
On Chip

Highly monodisperse and hydrolytically degradable thermoresponsive microgels on the tens-to-hundreds of micron size scale have been fabricated based on simultaneous on-chip mixing and emulsification of aldehyde and hydrazide-functionalized poly(N-isopropylacrylamide) precursor polymers.

Viscosity measurements on micron-size scale using optical tweezers

2005 ◽  
Vol 76 (11) ◽  
pp. 115105 ◽  
Author(s):  
G. Pesce ◽  
A. Sasso ◽  
S. Fusco
Keyword(s):  
Optical Tweezers ◽  
Size Scale ◽  
Viscosity Measurements ◽  
Micron Size

scholarly journals Lamin B distribution and association with peripheral chromatin revealed by optical sectioning and electron microscopy tomography.

1993 ◽  
Vol 123 (6) ◽  
pp. 1671-1685 ◽  
Author(s):  
A S Belmont ◽  
Y Zhai ◽  
A Thilenius
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Large Scale ◽  
Light And Electron Microscopy ◽  
Optical Sectioning ◽  
Nuclear Pores ◽  
Immunogold Staining ◽  
Lamin B ◽  
Size Scale ◽  
Micron Size

We have used a combination of immunogold staining, optical sectioning light microscopy, intermediate voltage electron microscopy, and EM tomography to examine the distribution of lamin B over the nuclear envelope of CHO cells. Apparent inconsistencies between previously published light and electron microscopy studies of nuclear lamin staining were resolved. At light microscopy resolution, an apparent open fibrillar network is visualized. Colocalization of lamin B and nuclear pores demonstrates that these apparent fibrils, separated by roughly 0.5 micron, are anti-correlated with the surface distribution of nuclear pores; pore clusters lie between or adjacent to regions of heavy lamin B staining. Examination at higher, EM resolution reveals that this apparent lamin B network does not correspond to an actual network of widely spaced, discrete bundles of lamin filaments. Rather it reflects a quantitative variation in lamin staining over a roughly 0.5-micron size scale, superimposed on a more continuous but still complex distribution of lamin filaments, spatially heterogeneous on a 0.1-0.2-micron size scale. Interestingly, lamin B staining at this higher resolution is highly correlated to the underlying chromatin distribution. Heavy concentrations of lamin B directly "cap" the surface of envelope associated, large-scale chromatin domains.

Gut microbiome a promising target for management of respiratory diseases

2020 ◽  
Vol 477 (14) ◽  
pp. 2679-2696
Author(s):  
Riddhi Trivedi ◽  
Kalyani Barve
Keyword(s):  
Respiratory Diseases ◽  
New Technology ◽  
Bacterial Flora ◽  
Systemic Circulation ◽  
The Body ◽  
Lung Pathology ◽  
Promising Target ◽  
Current Therapies ◽  
Intestinal Microbial Flora ◽  
On Chip

The intestinal microbial flora has risen to be one of the important etiological factors in the development of diseases like colorectal cancer, obesity, diabetes, inflammatory bowel disease, anxiety and Parkinson's. The emergence of the association between bacterial flora and lungs led to the discovery of the gut–lung axis. Dysbiosis of several species of colonic bacteria such as Firmicutes and Bacteroidetes and transfer of these bacteria from gut to lungs via lymphatic and systemic circulation are associated with several respiratory diseases such as lung cancer, asthma, tuberculosis, cystic fibrosis, etc. Current therapies for dysbiosis include use of probiotics, prebiotics and synbiotics to restore the balance between various species of beneficial bacteria. Various approaches like nanotechnology and microencapsulation have been explored to increase the permeability and viability of probiotics in the body. The need of the day is comprehensive study of mechanisms behind dysbiosis, translocation of microbiota from gut to lung through various channels and new technology for evaluating treatment to correct this dysbiosis which in turn can be used to manage various respiratory diseases. Microfluidics and organ on chip model are emerging technologies that can satisfy these needs. This review gives an overview of colonic commensals in lung pathology and novel systems that help in alleviating symptoms of lung diseases. We have also hypothesized new models to help in understanding bacterial pathways involved in the gut–lung axis as well as act as a futuristic approach in finding treatment of respiratory diseases caused by dysbiosis.

Review of Microfluidic Devices for On-Chip Chemical Sensing

2016 ◽  
Vol 136 (6) ◽  
pp. 244-249
Author(s):  
Takahiro Watanabe ◽  
Fumihiro Sassa ◽  
Yoshitaka Yoshizumi ◽  
Hiroaki Suzuki
Keyword(s):  
Chemical Sensing ◽  
Microfluidic Devices ◽  
On Chip
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