Oligodots: Structurally Defined Fluorescent Nanoprobes for Multiscale Dual-Color Imaging in Vitro and in Vivo

2020 ◽  
Vol 12 (9) ◽  
pp. 10183-10192
Author(s):  
Fatemeh Ostadhossein ◽  
Dinabandhu Sar ◽  
Indu Tripathi ◽  
Julio Soares ◽  
Edward E. Remsen ◽  
...  
Keyword(s):  
Color Imaging ◽  
Dual Color

scholarly journals CaRuby-Nano: a novel high affinity calcium probe for dual color imaging

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Mayeul Collot ◽  
Christian D Wilms ◽  
Asma Bentkhayet ◽  
Païkan Marcaggi ◽  
Kiri Couchman ◽  
...  
Keyword(s):  
Cell Biology ◽  
High Sensitivity ◽  
High Signal ◽  
Color Imaging ◽  
In Vivo Experiments ◽  
Dual Color ◽  
Calcium Indicator ◽  
Neuroscience Research

The great demand for long-wavelength and high signal-to-noise Ca2+ indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.

scholarly journals Dual Color Imaging from a Single BF2-Azadipyrromethene Fluorophore Demonstrated in vivo for Lymph Node Identification

2020 ◽  
Author(s):  
Niamh Curtin ◽  
Dan Wu ◽  
Ronan A. Cahill ◽  
Anwesha Sarkar ◽  
Pól Mac Aonghusa ◽  
...  
Keyword(s):  
Lymph Node ◽  
Fluorescence Imaging ◽  
Color Imaging ◽  
Lymph Node Mapping ◽  
Guided Surgery ◽  
Dual Color ◽  
Fluorescence Guided Surgery ◽  
Clinical Systems

<p>Dual color fluorescence imaging has been achieved in the highly complex biomedical scenario of lymph node mapping. Emissions at 700 and 800 nm can be achieved from a single fluorophore by establishing molecular and aggregate forms. Fluorophore was compatible with clinical systems for fluorescence guided surgery and no toxicity was observed in high dosage testing.</p>

scholarly journals Real-time In vivo Dual-color Imaging of Intracapillary Cancer Cell and Nucleus Deformation and Migration

2005 ◽  
Vol 65 (10) ◽  
pp. 4246-4252 ◽  
Author(s):  
Kensuke Yamauchi ◽  
Meng Yang ◽  
Ping Jiang ◽  
Norio Yamamoto ◽  
Mingxu Xu ◽  
...  
Keyword(s):  
Real Time ◽  
Cancer Cell ◽  
Color Imaging ◽  
Dual Color ◽  
And Migration

scholarly journals Mini-III RNase-based dual-color system for in vivo mRNA tracking

Development ◽  
2020 ◽  
Vol 147 (22) ◽  
pp. dev190728
Author(s):  
Lin Zhang ◽  
Luxi Chen ◽  
Jing Chen ◽  
Weimin Shen ◽  
Anming Meng
Keyword(s):  
Fluorescent Protein ◽  
Binding Activity ◽  
Nuclear Localization Sequence ◽  
Binding Property ◽  
Zebrafish Embryos ◽  
Target Mrnas ◽  
Dual Color ◽  
Color System

ABSTRACTMini-III RNase (mR3), a member of RNase III endonuclease family, can bind to and cleave double-stranded RNAs (dsRNAs). Inactive mR3 protein without the α5β-α6 loop loses the dsRNA cleavage activity, but retains dsRNA binding activity. Here, we establish an inactive mR3-based non-engineered mR3/dsRNA system for RNA tracking in zebrafish embryos. In vitro binding experiments show that inactive Staphylococcus epidermidis mR3 (dSmR3) protein possesses the highest binding affinity with dsRNAs among mR3s from other related species, and its binding property is retained in zebrafish embryos. Combined with a fluorescein-labeled antisense RNA probe recognizing the target mRNAs, dSmR3 tagged with a nuclear localization sequence and a fluorescent protein could allow visualization of the dynamics of endogenous target mRNAs. The dSmR3/antisense probe dual-color system provides a new approach for tracking non-engineered RNAs in real-time, which will help understand how endogenous RNAs dynamically move during embryonic development.

scholarly journals Cellular Dynamics Visualized in Live Cells in Vitro and in Vivo by Differential Dual-Color Nuclear-Cytoplasmic Fluorescent-Protein Expression

2004 ◽  
Vol 64 (12) ◽  
pp. 4251-4256 ◽  
Author(s):  
Norio Yamamoto ◽  
Ping Jiang ◽  
Meng Yang ◽  
Mingxu Xu ◽  
Kensuke Yamauchi ◽  
...  
Keyword(s):  
Protein Expression ◽  
Fluorescent Protein ◽  
Live Cells ◽  
Cellular Dynamics ◽  
Dual Color

scholarly journals Identifying hetero-protein complexes in the nuclear envelope

2019 ◽  
Author(s):  
J. Hennen ◽  
K.H. Hur ◽  
J. Kohler ◽  
S.R. Karuka ◽  
I. Angert ◽  
...  
Keyword(s):  
Nuclear Envelope ◽  
Protein Complexes ◽  
Living Cells ◽  
Model Systems ◽  
Linc Complex ◽  
Oligomeric State ◽  
Subcellular Compartment ◽  
Dual Color

AbstractThe nucleus is delineated by the nuclear envelope (NE), which is a double membrane barrier composed of the inner and outer nuclear membranes as well as a ~40 nm wide lumen. In addition to its barrier function, the NE acts as a critical signaling node for a variety of cellular processes which are mediated by protein complexes within this subcellular compartment. While fluorescence fluctuation spectroscopy (FFS) is a powerful tool for characterizing protein complexes in living cells, it was recently demonstrated that conventional FFS methods are not suitable for applications in the NE because of the presence of slow nuclear membrane undulations. We previously addressed this challenge by developing time-shifted mean-segmented Q (tsMSQ) analysis and applied it to successfully characterize protein homo-oligomerization in the NE. However, many NE complexes, such as the linker of the nucleoskeleton and cytoskeleton (LINC) complex, are formed by heterotypic interactions, which single-color tsMSQ is unable to characterize. Here, we describe the development of dual-color (DC) tsMSQ to analyze NE hetero-protein complexes built from proteins that carry two spectrally distinct fluorescent labels. Experiments performed on model systems demonstrate that DC tsMSQ properly identifies hetero-protein complexes and their stoichiometry in the NE by accounting for spectral crosstalk and local volume fluctuations. Finally, we applied DC tsMSQ to study the assembly of the LINC complex, a hetero-protein complex composed of Klarsicht/ANC-1/SYNE homology (KASH) and Sad1/UNC-84 (SUN) proteins, in the NE of living cells. Using DC tsMSQ, we demonstrate the ability of the SUN protein SUN2 and the KASH protein nesprin-2 to form a hetero-complex in vivo. Our results are consistent with previously published in vitro studies and demonstrate the utility of the DC tsMSQ technique for characterizing NE hetero-protein complexes.Statement of SignificanceProtein complexes found within the nuclear envelope (NE) play a vital role in regulating cellular functions ranging from gene expression to cellular movement. However, the assembly state of these complexes within their native environment remains poorly understood, which is compounded by a general lack of fluorescence techniques suitable for quantifying the oligomeric state of NE protein complexes. This study aims at addressing this issue by introducing dual-color time-shifted mean-segmented Q analysis as a fluorescence fluctuation method specifically designed to identify the average oligomeric state of hetero-protein complexes within the NE of living cells.

scholarly journals Dual Color Imaging from a Single BF2-Azadipyrromethene Fluorophore Demonstrated in vivo for Lymph Node Identification

2021 ◽  
Vol 18 (7) ◽  
pp. 1541-1553
Author(s):  
Niamh Curtin ◽  
Dan Wu ◽  
Ronan Cahill ◽  
Anwesha Sarkar ◽  
Pól Mac Aonghusa ◽  
...  
Keyword(s):  
Lymph Node ◽  
Color Imaging ◽  
Dual Color

scholarly journals Dual Color Imaging from a Single BF2-Azadipyrromethene Fluorophore Demonstrated in vivo for Lymph Node Identification

2020 ◽  
Author(s):  
Niamh Curtin ◽  
Dan Wu ◽  
Ronan A. Cahill ◽  
Anwesha Sarkar ◽  
Pól Mac Aonghusa ◽  
...  
Keyword(s):  
Lymph Node ◽  
Fluorescence Imaging ◽  
Color Imaging ◽  
Lymph Node Mapping ◽  
Guided Surgery ◽  
Dual Color ◽  
Fluorescence Guided Surgery ◽  
Clinical Systems

<p>Dual color fluorescence imaging has been achieved in the highly complex biomedical scenario of lymph node mapping. Emissions at 700 and 800 nm can be achieved from a single fluorophore by establishing molecular and aggregate forms. Fluorophore was compatible with clinical systems for fluorescence guided surgery and no toxicity was observed in high dosage testing.</p>

Sign in / Sign up

Export Citation Format

Share Document